Caulobacter phage Sansa
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 115 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K1LLY3|A0A0K1LLY3_9CAUD Uncharacterized protein OS=Caulobacter phage Sansa OX=1675600 GN=CPT_Sansa118 PE=4 SV=1
MM1 pKa = 8.16 PINKK5 pKa = 9.34 HH6 pKa = 4.85 YY7 pKa = 10.71 AVPGQPAVIVYY18 pKa = 10.42 KK19 pKa = 10.85 DD20 pKa = 3.8 GVPSARR26 pKa = 11.84 WPLDD30 pKa = 3.59 AGPGAHH36 pKa = 6.8 ACAAEE41 pKa = 3.99 WAQQYY46 pKa = 10.28 GGEE49 pKa = 4.14 VHH51 pKa = 6.99 EE52 pKa = 4.73 YY53 pKa = 10.64 DD54 pKa = 4.5 GSLEE58 pKa = 4.71 NSDD61 pKa = 3.31 AWHH64 pKa = 6.68 YY65 pKa = 11.26 DD66 pKa = 2.48 ADD68 pKa = 3.83 ARR70 pKa = 11.84 QLRR73 pKa = 11.84 DD74 pKa = 3.55 GNGLPVASDD83 pKa = 3.34 VGPADD88 pKa = 3.21 ARR90 pKa = 11.84 TILSMQRR97 pKa = 11.84 ALFEE101 pKa = 4.29 MVDD104 pKa = 3.48 TVPRR108 pKa = 11.84 TGAVADD114 pKa = 4.83 AYY116 pKa = 10.88 NDD118 pKa = 3.67 CARR121 pKa = 11.84 ISLAGLRR128 pKa = 11.84 GEE130 pKa = 4.22 MCPAEE135 pKa = 4.48 DD136 pKa = 4.66 AAAEE140 pKa = 4.15 DD141 pKa = 4.6 PEE143 pKa = 4.29 AEE145 pKa = 4.33 RR146 pKa = 11.84 IGEE149 pKa = 4.1 VLTRR153 pKa = 11.84 FLSYY157 pKa = 10.0 EE158 pKa = 3.67 AQAFHH163 pKa = 7.62 EE164 pKa = 4.66 DD165 pKa = 3.85 TPIDD169 pKa = 3.98 LADD172 pKa = 3.67 LCTFIGEE179 pKa = 4.17 VRR181 pKa = 11.84 QDD183 pKa = 3.48 LKK185 pKa = 11.15 RR186 pKa = 11.84 AMLSRR191 pKa = 11.84 DD192 pKa = 3.5 LATLPTAAEE201 pKa = 3.85 LRR203 pKa = 11.84 IHH205 pKa = 6.83 IKK207 pKa = 10.59 NGGVTDD213 pKa = 4.12 VEE215 pKa = 4.57 ASTEE219 pKa = 4.24 LKK221 pKa = 10.72 GLTIVTLDD229 pKa = 3.43 YY230 pKa = 11.64 DD231 pKa = 3.72 NDD233 pKa = 3.31 FTARR237 pKa = 11.84 GLLHH241 pKa = 6.27 MVNAAPDD248 pKa = 3.85 GEE250 pKa = 4.43 EE251 pKa = 3.93 EE252 pKa = 4.15 NFRR255 pKa = 11.84 PVYY258 pKa = 10.37 VVEE261 pKa = 3.93 QTPMVRR267 pKa = 11.84 ALPPILDD274 pKa = 4.21 AEE276 pKa = 4.67 DD277 pKa = 4.06 DD278 pKa = 3.68 QFDD281 pKa = 4.17 AMFEE285 pKa = 3.94 EE286 pKa = 5.21 DD287 pKa = 3.45
Molecular weight: 31.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.286
IPC2_protein 4.215
IPC_protein 4.202
Toseland 4.012
ProMoST 4.304
Dawson 4.164
Bjellqvist 4.329
Wikipedia 4.062
Rodwell 4.024
Grimsley 3.923
Solomon 4.164
Lehninger 4.126
Nozaki 4.279
DTASelect 4.482
Thurlkill 4.037
EMBOSS 4.075
Sillero 4.317
Patrickios 3.554
IPC_peptide 4.164
IPC2_peptide 4.304
IPC2.peptide.svr19 4.237
Protein with the highest isoelectric point:
>tr|A0A0K1LMP2|A0A0K1LMP2_9CAUD Uncharacterized protein OS=Caulobacter phage Sansa OX=1675600 GN=CPT_Sansa64 PE=4 SV=1
MM1 pKa = 6.93 TRR3 pKa = 11.84 VSGRR7 pKa = 11.84 YY8 pKa = 8.37 PRR10 pKa = 11.84 IPCVVFGCKK19 pKa = 9.74 CGATCYY25 pKa = 10.01 PPGVEE30 pKa = 4.31 IICPKK35 pKa = 10.17 HH36 pKa = 5.56 YY37 pKa = 10.62 RR38 pKa = 11.84 LVDD41 pKa = 3.43 KK42 pKa = 10.71 SLKK45 pKa = 9.53 RR46 pKa = 11.84 LRR48 pKa = 11.84 RR49 pKa = 11.84 EE50 pKa = 3.25 IRR52 pKa = 11.84 RR53 pKa = 11.84 AFIKK57 pKa = 10.56 SGAGQSPSRR66 pKa = 11.84 WIFEE70 pKa = 4.15 DD71 pKa = 3.74 LLWRR75 pKa = 11.84 MIVEE79 pKa = 4.39 QATHH83 pKa = 6.57 RR84 pKa = 11.84 AAGII88 pKa = 3.65
Molecular weight: 10.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.342
IPC2_protein 9.502
IPC_protein 10.043
Toseland 10.35
ProMoST 10.043
Dawson 10.511
Bjellqvist 10.233
Wikipedia 10.687
Rodwell 10.745
Grimsley 10.555
Solomon 10.584
Lehninger 10.555
Nozaki 10.438
DTASelect 10.204
Thurlkill 10.379
EMBOSS 10.76
Sillero 10.452
Patrickios 10.496
IPC_peptide 10.584
IPC2_peptide 9.589
IPC2.peptide.svr19 8.369
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
115
0
115
24848
47
1801
216.1
23.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.572 ± 0.532
0.893 ± 0.095
6.495 ± 0.199
6.395 ± 0.24
3.107 ± 0.113
7.956 ± 0.252
1.783 ± 0.138
4.648 ± 0.165
4.419 ± 0.188
8.395 ± 0.197
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.535 ± 0.129
3.042 ± 0.177
5.497 ± 0.348
3.69 ± 0.254
6.991 ± 0.238
5.32 ± 0.225
5.377 ± 0.244
6.89 ± 0.246
1.767 ± 0.123
2.226 ± 0.113
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here