Lepus americanus faeces associated microvirus SHP1 6472

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5CJ84|A0A2Z5CJ84_9VIRU Major capsid protein OS=Lepus americanus faeces associated microvirus SHP1 6472 OX=2219217 PE=3 SV=1
MM1 pKa = 7.4SGVFDD6 pKa = 3.7SLKK9 pKa = 10.89NVTNPINRR17 pKa = 11.84NTFDD21 pKa = 3.25WSHH24 pKa = 5.85VNNFTTGFGRR34 pKa = 11.84LTPVLCEE41 pKa = 3.75YY42 pKa = 10.6CPPNTSLRR50 pKa = 11.84IKK52 pKa = 9.76PQFGLRR58 pKa = 11.84FMPMMFPVQTRR69 pKa = 11.84IKK71 pKa = 10.12AYY73 pKa = 9.8LSFYY77 pKa = 10.01RR78 pKa = 11.84VPIRR82 pKa = 11.84TLWKK86 pKa = 10.24DD87 pKa = 3.3YY88 pKa = 10.96KK89 pKa = 11.03DD90 pKa = 3.65WVSSANDD97 pKa = 3.3QTSDD101 pKa = 4.5LEE103 pKa = 4.29PPYY106 pKa = 10.82LDD108 pKa = 5.44LPIDD112 pKa = 4.35SYY114 pKa = 11.36TSGYY118 pKa = 8.36MRR120 pKa = 11.84PSGLADD126 pKa = 3.38YY127 pKa = 11.03LGIPNDD133 pKa = 3.14ITFNDD138 pKa = 3.88SDD140 pKa = 4.11LSSWTISTVSQLNTSNTSSLVNTKK164 pKa = 10.24DD165 pKa = 3.32YY166 pKa = 11.55YY167 pKa = 11.28FFGVSNLMNPAINTGNGIFSYY188 pKa = 10.31EE189 pKa = 3.8LNYY192 pKa = 9.89QRR194 pKa = 11.84NISGYY199 pKa = 8.04PNSAYY204 pKa = 10.84VLATFSQPTFNFRR217 pKa = 11.84GTLKK221 pKa = 10.65FSFSTTQSSAEE232 pKa = 4.02AIINDD237 pKa = 3.49VKK239 pKa = 11.13KK240 pKa = 10.63NKK242 pKa = 8.28TWSICLSTFGQQAAQYY258 pKa = 10.29YY259 pKa = 9.91NYY261 pKa = 10.59TNINPILTVSSSMLSIEE278 pKa = 4.43NNTDD282 pKa = 2.97SDD284 pKa = 4.18STSKK288 pKa = 10.52PKK290 pKa = 10.59KK291 pKa = 9.97LVLTIPSFSFSVSSVPTNSGIVFQYY316 pKa = 10.42RR317 pKa = 11.84SLMLFWRR324 pKa = 11.84DD325 pKa = 2.92HH326 pKa = 6.65SYY328 pKa = 11.35NGSNNDD334 pKa = 3.18EE335 pKa = 4.1NSIKK339 pKa = 10.3EE340 pKa = 3.8FSITQSTNSNLSNQPYY356 pKa = 9.74YY357 pKa = 11.06SSDD360 pKa = 3.18LSAPDD365 pKa = 3.12AKK367 pKa = 11.06YY368 pKa = 10.47GIKK371 pKa = 10.43LSAYY375 pKa = 8.31PFRR378 pKa = 11.84AYY380 pKa = 9.9EE381 pKa = 3.95AVYY384 pKa = 10.26NAYY387 pKa = 9.4IRR389 pKa = 11.84NNKK392 pKa = 8.75NNPFYY397 pKa = 10.59IDD399 pKa = 4.86DD400 pKa = 3.79KK401 pKa = 11.06VSYY404 pKa = 10.35NKK406 pKa = 9.86WIPNDD411 pKa = 3.22NGGADD416 pKa = 3.03KK417 pKa = 10.95YY418 pKa = 11.09AYY420 pKa = 9.47QLHH423 pKa = 6.3HH424 pKa = 7.12SNWASDD430 pKa = 3.66QFTTAVPSPQQGQAPLVGITTYY452 pKa = 10.14TKK454 pKa = 10.21TVALEE459 pKa = 4.25NGHH462 pKa = 6.24SEE464 pKa = 4.43TTVGTALVDD473 pKa = 3.7EE474 pKa = 5.74DD475 pKa = 4.07GNKK478 pKa = 10.19YY479 pKa = 10.3DD480 pKa = 4.91VSYY483 pKa = 10.86EE484 pKa = 4.3SNGDD488 pKa = 3.38EE489 pKa = 4.69LKK491 pKa = 10.78DD492 pKa = 3.82VKK494 pKa = 10.31YY495 pKa = 9.14TLLNADD501 pKa = 3.62TPVRR505 pKa = 11.84PVSNLFDD512 pKa = 4.26LVTSGISINDD522 pKa = 3.28FRR524 pKa = 11.84NVNAYY529 pKa = 8.79QRR531 pKa = 11.84YY532 pKa = 9.51LEE534 pKa = 4.28LNQMRR539 pKa = 11.84GYY541 pKa = 9.64SYY543 pKa = 11.36KK544 pKa = 10.81DD545 pKa = 2.93IVEE548 pKa = 4.05GRR550 pKa = 11.84FDD552 pKa = 3.79VNVRR556 pKa = 11.84YY557 pKa = 10.13DD558 pKa = 3.95DD559 pKa = 5.05LNMPEE564 pKa = 4.32YY565 pKa = 10.43LGGMTRR571 pKa = 11.84DD572 pKa = 3.61VSISPVTQTQAITPGSWQEE591 pKa = 4.07GTYY594 pKa = 8.88STQLGAQAGNGTLFADD610 pKa = 5.06GEE612 pKa = 4.55NISCFCDD619 pKa = 3.1EE620 pKa = 4.33EE621 pKa = 5.43SFVIGILSVCPMPVYY636 pKa = 9.78TQVLPKK642 pKa = 10.4HH643 pKa = 5.64FLYY646 pKa = 10.35RR647 pKa = 11.84DD648 pKa = 3.44RR649 pKa = 11.84LDD651 pKa = 3.52YY652 pKa = 9.95FAPEE656 pKa = 3.75FDD658 pKa = 4.86HH659 pKa = 8.47IGFQPIPLKK668 pKa = 10.62EE669 pKa = 3.7IAPINTYY676 pKa = 8.36FTLGRR681 pKa = 11.84NHH683 pKa = 7.3LDD685 pKa = 3.18DD686 pKa = 3.57TFGYY690 pKa = 8.8QRR692 pKa = 11.84PWYY695 pKa = 8.6EE696 pKa = 3.89FCQKK700 pKa = 10.69LDD702 pKa = 3.52TAHH705 pKa = 7.12GLFKK709 pKa = 10.61TDD711 pKa = 4.46LRR713 pKa = 11.84NFLINRR719 pKa = 11.84VFGGVPSLNSAFTTMDD735 pKa = 3.58EE736 pKa = 4.19NDD738 pKa = 4.09LNDD741 pKa = 3.46VFAVTDD747 pKa = 3.59ISDD750 pKa = 4.51KK751 pKa = 10.88ILGAIWFDD759 pKa = 3.28ITAKK763 pKa = 10.72LPISRR768 pKa = 11.84VSVPRR773 pKa = 11.84LEE775 pKa = 4.1

Molecular weight: 87.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5CJ84|A0A2Z5CJ84_9VIRU Major capsid protein OS=Lepus americanus faeces associated microvirus SHP1 6472 OX=2219217 PE=3 SV=1
MM1 pKa = 7.13FCEE4 pKa = 4.23NPVILLNPKK13 pKa = 9.11LRR15 pKa = 11.84KK16 pKa = 8.61LTVHH20 pKa = 7.1CDD22 pKa = 3.55YY23 pKa = 10.44MHH25 pKa = 7.03SPLGDD30 pKa = 3.29YY31 pKa = 11.09SLLDD35 pKa = 3.17KK36 pKa = 10.98RR37 pKa = 11.84LFDD40 pKa = 4.11FRR42 pKa = 11.84RR43 pKa = 11.84VRR45 pKa = 11.84PKK47 pKa = 10.29ICDD50 pKa = 3.36KK51 pKa = 10.13LTKK54 pKa = 10.3DD55 pKa = 3.29NYY57 pKa = 10.9NEE59 pKa = 3.9YY60 pKa = 10.36FVANSSTGEE69 pKa = 4.11TFPLYY74 pKa = 10.39ILVPCGKK81 pKa = 10.23CPLCRR86 pKa = 11.84LTKK89 pKa = 10.36KK90 pKa = 10.64NEE92 pKa = 3.67LAFRR96 pKa = 11.84CVAEE100 pKa = 4.29TNRR103 pKa = 11.84FDD105 pKa = 3.94TLPLFITLTYY115 pKa = 11.04NNAALPDD122 pKa = 4.11CGVCKK127 pKa = 10.57ADD129 pKa = 3.2VQRR132 pKa = 11.84FLKK135 pKa = 10.41RR136 pKa = 11.84LRR138 pKa = 11.84ARR140 pKa = 11.84LDD142 pKa = 3.41YY143 pKa = 11.16EE144 pKa = 4.48KK145 pKa = 10.33IEE147 pKa = 4.17HH148 pKa = 6.14NLRR151 pKa = 11.84YY152 pKa = 9.99LCVSEE157 pKa = 4.15YY158 pKa = 10.66GKK160 pKa = 10.84SFGRR164 pKa = 11.84PHH166 pKa = 7.2YY167 pKa = 10.95HH168 pKa = 5.19MLLWNFPVSAFPNISAVVHH187 pKa = 6.18FIEE190 pKa = 5.12RR191 pKa = 11.84AWSVYY196 pKa = 10.09LLSVPCSKK204 pKa = 10.4RR205 pKa = 11.84VSLSRR210 pKa = 11.84AGEE213 pKa = 3.99LFRR216 pKa = 11.84FTSGRR221 pKa = 11.84RR222 pKa = 11.84QFDD225 pKa = 3.47AVSLGFVYY233 pKa = 10.13VLPLKK238 pKa = 10.55EE239 pKa = 4.28GGVDD243 pKa = 3.63YY244 pKa = 10.95VLKK247 pKa = 11.29YY248 pKa = 10.04MMKK251 pKa = 10.63DD252 pKa = 2.99KK253 pKa = 11.21DD254 pKa = 3.84AVPCGKK260 pKa = 9.85NPLFMLSSRR269 pKa = 11.84KK270 pKa = 9.81NGGIGSEE277 pKa = 4.4YY278 pKa = 10.04IRR280 pKa = 11.84SQRR283 pKa = 11.84DD284 pKa = 3.22YY285 pKa = 11.06FLSAKK290 pKa = 10.48NITTLSIVDD299 pKa = 3.76KK300 pKa = 10.18LVSGRR305 pKa = 11.84VVKK308 pKa = 9.62TKK310 pKa = 8.4ITPYY314 pKa = 10.02IKK316 pKa = 10.26SLLMPSPSRR325 pKa = 11.84KK326 pKa = 9.08YY327 pKa = 10.01RR328 pKa = 11.84RR329 pKa = 11.84KK330 pKa = 9.86EE331 pKa = 3.61YY332 pKa = 11.07NLMKK336 pKa = 10.12EE337 pKa = 4.97FCQKK341 pKa = 10.45LSKK344 pKa = 10.54FMNLCVKK351 pKa = 10.39FNEE354 pKa = 4.42LYY356 pKa = 10.75KK357 pKa = 10.53PIGYY361 pKa = 9.42SRR363 pKa = 11.84EE364 pKa = 4.18TMDD367 pKa = 4.18EE368 pKa = 4.18YY369 pKa = 11.67YY370 pKa = 10.83EE371 pKa = 4.55PFTDD375 pKa = 3.89IYY377 pKa = 11.15SRR379 pKa = 11.84PIWTEE384 pKa = 3.4TLRR387 pKa = 11.84KK388 pKa = 9.49CRR390 pKa = 11.84FLGYY394 pKa = 9.81VPEE397 pKa = 4.93RR398 pKa = 11.84EE399 pKa = 4.04VDD401 pKa = 5.03DD402 pKa = 4.87DD403 pKa = 4.55YY404 pKa = 12.32SWIKK408 pKa = 11.11DD409 pKa = 3.16KK410 pKa = 10.72DD411 pKa = 3.32TWNQFAYY418 pKa = 10.06EE419 pKa = 4.29CLDD422 pKa = 3.52EE423 pKa = 6.05LEE425 pKa = 4.33NLCIKK430 pKa = 10.47ILDD433 pKa = 3.84IPDD436 pKa = 3.05YY437 pKa = 10.8NAYY440 pKa = 10.22FDD442 pKa = 5.56ARR444 pKa = 11.84EE445 pKa = 3.75QYY447 pKa = 11.04LNEE450 pKa = 4.65RR451 pKa = 11.84GLQLEE456 pKa = 4.24YY457 pKa = 9.94EE458 pKa = 4.61YY459 pKa = 11.6CNMTPADD466 pKa = 3.92PHH468 pKa = 7.13VLADD472 pKa = 4.27KK473 pKa = 10.43IRR475 pKa = 11.84YY476 pKa = 8.97SMARR480 pKa = 11.84ALTKK484 pKa = 10.6EE485 pKa = 4.29KK486 pKa = 10.51II487 pKa = 3.62

Molecular weight: 57.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1626

364

775

542.0

61.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.027 ± 1.663

1.722 ± 0.714

6.335 ± 0.319

4.428 ± 0.754

4.859 ± 0.887

5.228 ± 0.488

1.107 ± 0.178

5.72 ± 0.562

5.781 ± 1.115

9.41 ± 1.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.03 ± 0.22

7.196 ± 0.921

4.613 ± 1.061

3.813 ± 1.105

4.797 ± 0.936

9.287 ± 1.147

5.904 ± 1.237

5.535 ± 0.359

1.046 ± 0.286

5.166 ± 1.278
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski