Halomarina oriensis

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Halobacteriaceae; Halomarina

Average proteome isoelectric point is 5.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3340 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P3RE93|A0A3P3RE93_9EURY Right-handed parallel beta-helix repeat-containing protein OS=Halomarina oriensis OX=671145 GN=EIK79_06035 PE=4 SV=1
MM1 pKa = 7.04TQDD4 pKa = 3.08NHH6 pKa = 5.91NTDD9 pKa = 3.37TTDD12 pKa = 3.86DD13 pKa = 3.83RR14 pKa = 11.84STDD17 pKa = 3.34GTRR20 pKa = 11.84SSWHH24 pKa = 6.09RR25 pKa = 11.84LSGVFEE31 pKa = 4.61DD32 pKa = 3.6SSRR35 pKa = 11.84RR36 pKa = 11.84EE37 pKa = 3.85FLRR40 pKa = 11.84AGAAIGAGGLALGLGNTATAAKK62 pKa = 10.38VEE64 pKa = 4.69EE65 pKa = 4.7ICNSDD70 pKa = 3.46YY71 pKa = 10.94GTIDD75 pKa = 3.25VADD78 pKa = 4.78GFTLMDD84 pKa = 4.14NRR86 pKa = 11.84WGNQNAHH93 pKa = 4.2QCIWYY98 pKa = 9.9NDD100 pKa = 3.96DD101 pKa = 3.3GSYY104 pKa = 11.06GYY106 pKa = 10.91DD107 pKa = 3.18FDD109 pKa = 5.73ASNTGGGINYY119 pKa = 9.21PEE121 pKa = 4.28VFIGTRR127 pKa = 11.84PWGASTGVVEE137 pKa = 6.68FPIQRR142 pKa = 11.84HH143 pKa = 5.52DD144 pKa = 3.34VDD146 pKa = 4.0EE147 pKa = 4.79FVMEE151 pKa = 4.3VDD153 pKa = 3.65ADD155 pKa = 4.12LDD157 pKa = 3.66ISGGEE162 pKa = 3.95WDD164 pKa = 4.08WAEE167 pKa = 4.21EE168 pKa = 3.91WWLMEE173 pKa = 4.74QPPTQQPEE181 pKa = 4.02THH183 pKa = 6.15QYY185 pKa = 11.07EE186 pKa = 4.69VMLLLDD192 pKa = 3.28WGGGHH197 pKa = 7.03DD198 pKa = 4.39HH199 pKa = 7.06GAVQDD204 pKa = 3.99PSAWTDD210 pKa = 3.08RR211 pKa = 11.84FGNTIDD217 pKa = 3.84LWTTYY222 pKa = 11.25DD223 pKa = 3.62SGGTDD228 pKa = 2.76AYY230 pKa = 10.75FYY232 pKa = 10.59IFRR235 pKa = 11.84IQGGHH240 pKa = 7.13DD241 pKa = 3.18GGKK244 pKa = 9.22IDD246 pKa = 3.45MAEE249 pKa = 3.67IVEE252 pKa = 4.24YY253 pKa = 10.65LSSNEE258 pKa = 3.83GVRR261 pKa = 11.84GDD263 pKa = 3.5LWLSGIEE270 pKa = 4.61LGNEE274 pKa = 3.66YY275 pKa = 9.66WAGAVGEE282 pKa = 4.42TTYY285 pKa = 10.22NTFNVTINGSTYY297 pKa = 10.55TSGTGDD303 pKa = 3.5GGSPPTDD310 pKa = 3.41TPTDD314 pKa = 3.78TPTDD318 pKa = 3.68TPGDD322 pKa = 3.57GLVINDD328 pKa = 3.83YY329 pKa = 11.26DD330 pKa = 4.87GDD332 pKa = 4.35PSWSSNTNDD341 pKa = 3.13LGQWCGAGSFANGSGTVSNGALVLEE366 pKa = 4.36YY367 pKa = 10.98DD368 pKa = 3.15NGGWYY373 pKa = 9.47QEE375 pKa = 4.04QLNQSVTDD383 pKa = 3.47YY384 pKa = 9.62STLVLRR390 pKa = 11.84VSGASGGEE398 pKa = 3.83EE399 pKa = 3.61SDD401 pKa = 3.25ITVEE405 pKa = 4.01MGGVSAPLADD415 pKa = 3.67VTSDD419 pKa = 4.02SIATNMSDD427 pKa = 4.38VAIDD431 pKa = 3.79LQAAGVDD438 pKa = 3.76RR439 pKa = 11.84SSSSLSLRR447 pKa = 11.84LNFWGGGSSTLQIEE461 pKa = 4.76EE462 pKa = 4.02IALVV466 pKa = 3.47

Molecular weight: 50.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P3RJY3|A0A3P3RJY3_9EURY GYD domain-containing protein OS=Halomarina oriensis OX=671145 GN=EIK79_03640 PE=4 SV=1
MM1 pKa = 7.56SEE3 pKa = 4.35RR4 pKa = 11.84EE5 pKa = 4.13SSVTRR10 pKa = 11.84PRR12 pKa = 11.84SDD14 pKa = 3.2SKK16 pKa = 10.63PLQWLFDD23 pKa = 4.18PSLLALCGVSVLLVAVGGLVQVSFGSYY50 pKa = 8.86QMSLTEE56 pKa = 3.57VWQAVFNPAVLFDD69 pKa = 4.66LRR71 pKa = 11.84WLSKK75 pKa = 10.61FILGDD80 pKa = 3.41SVMRR84 pKa = 11.84SITGFTGTLPEE95 pKa = 5.5LDD97 pKa = 3.45TSTTVVWNIRR107 pKa = 11.84LPRR110 pKa = 11.84VLLAVFVGMNLAVSGAIFQAVTRR133 pKa = 11.84NEE135 pKa = 3.94LASPFVLGISHH146 pKa = 7.18GAGLVILLTLVVFTGLTAWLPIVAALGGAVAFTIVYY182 pKa = 10.05AIAWKK187 pKa = 10.63NGTSPVRR194 pKa = 11.84LVLAGIIVATVFQSLQRR211 pKa = 11.84SLFFFATDD219 pKa = 2.64IGTAQTAMAWMTGSLTGVGWTQFRR243 pKa = 11.84IALPWTILVMALSIISARR261 pKa = 11.84QLNVLLLGQRR271 pKa = 11.84TAKK274 pKa = 10.1SLGMSVEE281 pKa = 3.99RR282 pKa = 11.84VRR284 pKa = 11.84FALSGVAVLAAAVSIAVAGVVSFVGLIVPHH314 pKa = 6.29LVRR317 pKa = 11.84TLVGNDD323 pKa = 3.21HH324 pKa = 6.84KK325 pKa = 11.28KK326 pKa = 11.02LIVGCLFTGPALMVVADD343 pKa = 3.74VGARR347 pKa = 11.84LGPSILIGAANQQLPVGVVTGLIGGPYY374 pKa = 9.99FLYY377 pKa = 10.82LMRR380 pKa = 11.84KK381 pKa = 8.13RR382 pKa = 11.84QQLGDD387 pKa = 3.51VV388 pKa = 3.84

Molecular weight: 41.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3340

0

3340

990830

32

1653

296.7

32.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.49 ± 0.047

0.846 ± 0.015

7.56 ± 0.045

7.822 ± 0.047

3.355 ± 0.029

7.917 ± 0.038

2.313 ± 0.021

5.064 ± 0.033

2.125 ± 0.021

8.864 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.818 ± 0.016

2.843 ± 0.027

4.649 ± 0.024

3.116 ± 0.025

6.387 ± 0.038

6.09 ± 0.032

7.338 ± 0.038

8.324 ± 0.038

1.204 ± 0.017

2.876 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski