Amino acid dipepetide frequency for Erwinia phage PEp14

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.89AlaAla: 17.89 ± 1.638
0.414AlaCys: 0.414 ± 0.177
6.929AlaAsp: 6.929 ± 0.502
8.273AlaGlu: 8.273 ± 0.908
3.464AlaPhe: 3.464 ± 0.352
11.789AlaGly: 11.789 ± 1.489
1.965AlaHis: 1.965 ± 0.329
4.86AlaIle: 4.86 ± 0.537
5.791AlaLys: 5.791 ± 0.601
7.911AlaLeu: 7.911 ± 0.659
3.464AlaMet: 3.464 ± 0.452
3.878AlaAsn: 3.878 ± 0.539
5.222AlaPro: 5.222 ± 0.616
5.843AlaGln: 5.843 ± 0.673
8.583AlaArg: 8.583 ± 1.049
5.636AlaSer: 5.636 ± 0.524
5.791AlaThr: 5.791 ± 0.489
7.187AlaVal: 7.187 ± 0.654
1.499AlaTrp: 1.499 ± 0.301
2.792AlaTyr: 2.792 ± 0.358
0.0AlaXaa: 0.0 ± 0.0
Cys
0.569CysAla: 0.569 ± 0.22
0.052CysCys: 0.052 ± 0.053
0.31CysAsp: 0.31 ± 0.166
0.465CysGlu: 0.465 ± 0.166
0.052CysPhe: 0.052 ± 0.06
0.362CysGly: 0.362 ± 0.171
0.103CysHis: 0.103 ± 0.082
0.155CysIle: 0.155 ± 0.105
0.207CysLys: 0.207 ± 0.1
0.569CysLeu: 0.569 ± 0.228
0.103CysMet: 0.103 ± 0.085
0.31CysAsn: 0.31 ± 0.146
0.465CysPro: 0.465 ± 0.215
0.155CysGln: 0.155 ± 0.089
0.62CysArg: 0.62 ± 0.258
0.362CysSer: 0.362 ± 0.177
0.155CysThr: 0.155 ± 0.094
0.569CysVal: 0.569 ± 0.219
0.362CysTrp: 0.362 ± 0.171
0.259CysTyr: 0.259 ± 0.127
0.0CysXaa: 0.0 ± 0.0
Asp
7.653AspAla: 7.653 ± 0.689
0.517AspCys: 0.517 ± 0.206
4.55AspAsp: 4.55 ± 0.541
4.343AspGlu: 4.343 ± 0.587
2.896AspPhe: 2.896 ± 0.365
6.153AspGly: 6.153 ± 0.503
0.879AspHis: 0.879 ± 0.214
2.585AspIle: 2.585 ± 0.357
2.74AspLys: 2.74 ± 0.37
4.602AspLeu: 4.602 ± 0.404
1.448AspMet: 1.448 ± 0.269
1.965AspAsn: 1.965 ± 0.337
2.947AspPro: 2.947 ± 0.385
2.172AspGln: 2.172 ± 0.315
4.343AspArg: 4.343 ± 0.666
2.12AspSer: 2.12 ± 0.318
3.206AspThr: 3.206 ± 0.451
3.878AspVal: 3.878 ± 0.446
1.086AspTrp: 1.086 ± 0.243
2.172AspTyr: 2.172 ± 0.318
0.0AspXaa: 0.0 ± 0.0
Glu
7.601GluAla: 7.601 ± 0.709
0.31GluCys: 0.31 ± 0.165
3.516GluAsp: 3.516 ± 0.552
5.584GluGlu: 5.584 ± 1.162
1.551GluPhe: 1.551 ± 0.276
4.86GluGly: 4.86 ± 0.601
1.448GluHis: 1.448 ± 0.26
2.999GluIle: 2.999 ± 0.346
3.102GluLys: 3.102 ± 0.426
6.515GluLeu: 6.515 ± 0.666
1.499GluMet: 1.499 ± 0.248
2.378GluAsn: 2.378 ± 0.325
4.137GluPro: 4.137 ± 0.601
4.55GluGln: 4.55 ± 0.68
5.119GluArg: 5.119 ± 0.771
2.585GluSer: 2.585 ± 0.359
3.206GluThr: 3.206 ± 0.477
4.447GluVal: 4.447 ± 0.406
1.138GluTrp: 1.138 ± 0.215
1.448GluTyr: 1.448 ± 0.33
0.0GluXaa: 0.0 ± 0.0
Phe
3.93PheAla: 3.93 ± 0.425
0.207PheCys: 0.207 ± 0.105
2.534PheAsp: 2.534 ± 0.408
2.327PheGlu: 2.327 ± 0.405
0.672PhePhe: 0.672 ± 0.179
2.74PheGly: 2.74 ± 0.398
0.776PheHis: 0.776 ± 0.217
1.448PheIle: 1.448 ± 0.276
1.086PheLys: 1.086 ± 0.181
1.965PheLeu: 1.965 ± 0.332
0.62PheMet: 0.62 ± 0.236
1.758PheAsn: 1.758 ± 0.341
1.086PhePro: 1.086 ± 0.25
0.931PheGln: 0.931 ± 0.154
1.499PheArg: 1.499 ± 0.262
2.068PheSer: 2.068 ± 0.385
2.172PheThr: 2.172 ± 0.391
2.43PheVal: 2.43 ± 0.321
0.517PheTrp: 0.517 ± 0.223
0.879PheTyr: 0.879 ± 0.202
0.0PheXaa: 0.0 ± 0.0
Gly
8.325GlyAla: 8.325 ± 1.132
0.362GlyCys: 0.362 ± 0.228
5.171GlyAsp: 5.171 ± 0.561
5.119GlyGlu: 5.119 ± 0.428
2.223GlyPhe: 2.223 ± 0.309
7.704GlyGly: 7.704 ± 0.933
1.344GlyHis: 1.344 ± 0.205
3.981GlyIle: 3.981 ± 0.378
5.016GlyLys: 5.016 ± 0.369
4.86GlyLeu: 4.86 ± 0.493
3.102GlyMet: 3.102 ± 0.499
3.309GlyAsn: 3.309 ± 0.506
2.378GlyPro: 2.378 ± 0.35
3.206GlyGln: 3.206 ± 0.435
5.274GlyArg: 5.274 ± 0.618
5.067GlySer: 5.067 ± 0.678
5.636GlyThr: 5.636 ± 0.689
4.447GlyVal: 4.447 ± 0.517
2.223GlyTrp: 2.223 ± 0.308
2.689GlyTyr: 2.689 ± 0.432
0.0GlyXaa: 0.0 ± 0.0
His
1.603HisAla: 1.603 ± 0.298
0.155HisCys: 0.155 ± 0.1
1.034HisAsp: 1.034 ± 0.215
1.396HisGlu: 1.396 ± 0.284
1.241HisPhe: 1.241 ± 0.309
1.81HisGly: 1.81 ± 0.415
0.362HisHis: 0.362 ± 0.13
0.931HisIle: 0.931 ± 0.204
0.155HisLys: 0.155 ± 0.077
0.931HisLeu: 0.931 ± 0.201
0.414HisMet: 0.414 ± 0.192
0.776HisAsn: 0.776 ± 0.209
0.879HisPro: 0.879 ± 0.213
0.879HisGln: 0.879 ± 0.191
1.344HisArg: 1.344 ± 0.285
1.241HisSer: 1.241 ± 0.244
0.879HisThr: 0.879 ± 0.258
1.034HisVal: 1.034 ± 0.209
0.31HisTrp: 0.31 ± 0.138
0.62HisTyr: 0.62 ± 0.152
0.0HisXaa: 0.0 ± 0.0
Ile
3.516IleAla: 3.516 ± 0.424
0.414IleCys: 0.414 ± 0.171
2.637IleAsp: 2.637 ± 0.525
4.55IleGlu: 4.55 ± 0.433
1.086IlePhe: 1.086 ± 0.277
2.689IleGly: 2.689 ± 0.363
0.879IleHis: 0.879 ± 0.189
1.603IleIle: 1.603 ± 0.21
1.551IleLys: 1.551 ± 0.328
2.43IleLeu: 2.43 ± 0.377
1.138IleMet: 1.138 ± 0.239
2.223IleAsn: 2.223 ± 0.333
2.275IlePro: 2.275 ± 0.408
1.396IleGln: 1.396 ± 0.317
2.689IleArg: 2.689 ± 0.344
2.327IleSer: 2.327 ± 0.297
2.585IleThr: 2.585 ± 0.355
2.172IleVal: 2.172 ± 0.384
0.362IleTrp: 0.362 ± 0.129
1.138IleTyr: 1.138 ± 0.276
0.0IleXaa: 0.0 ± 0.0
Lys
7.704LysAla: 7.704 ± 0.913
0.259LysCys: 0.259 ± 0.128
2.068LysAsp: 2.068 ± 0.328
2.223LysGlu: 2.223 ± 0.334
1.448LysPhe: 1.448 ± 0.227
3.413LysGly: 3.413 ± 0.403
0.724LysHis: 0.724 ± 0.219
1.499LysIle: 1.499 ± 0.335
2.792LysLys: 2.792 ± 0.415
3.619LysLeu: 3.619 ± 0.399
1.655LysMet: 1.655 ± 0.317
1.655LysAsn: 1.655 ± 0.257
3.568LysPro: 3.568 ± 0.424
1.913LysGln: 1.913 ± 0.307
3.568LysArg: 3.568 ± 0.435
1.706LysSer: 1.706 ± 0.295
2.999LysThr: 2.999 ± 0.358
3.464LysVal: 3.464 ± 0.375
0.724LysTrp: 0.724 ± 0.222
1.086LysTyr: 1.086 ± 0.241
0.0LysXaa: 0.0 ± 0.0
Leu
8.17LeuAla: 8.17 ± 0.782
0.465LeuCys: 0.465 ± 0.175
4.757LeuAsp: 4.757 ± 0.564
3.981LeuGlu: 3.981 ± 0.567
2.999LeuPhe: 2.999 ± 0.369
5.119LeuGly: 5.119 ± 0.388
1.448LeuHis: 1.448 ± 0.224
2.534LeuIle: 2.534 ± 0.44
3.981LeuLys: 3.981 ± 0.384
5.481LeuLeu: 5.481 ± 0.772
2.482LeuMet: 2.482 ± 0.381
3.568LeuAsn: 3.568 ± 0.392
4.705LeuPro: 4.705 ± 0.685
2.275LeuGln: 2.275 ± 0.374
5.016LeuArg: 5.016 ± 0.475
5.016LeuSer: 5.016 ± 0.454
4.964LeuThr: 4.964 ± 0.743
4.188LeuVal: 4.188 ± 0.506
1.189LeuTrp: 1.189 ± 0.38
2.017LeuTyr: 2.017 ± 0.298
0.0LeuXaa: 0.0 ± 0.0
Met
3.309MetAla: 3.309 ± 0.338
0.155MetCys: 0.155 ± 0.1
1.655MetAsp: 1.655 ± 0.323
1.499MetGlu: 1.499 ± 0.279
0.982MetPhe: 0.982 ± 0.171
1.758MetGly: 1.758 ± 0.284
0.569MetHis: 0.569 ± 0.161
0.879MetIle: 0.879 ± 0.209
1.499MetLys: 1.499 ± 0.275
2.017MetLeu: 2.017 ± 0.265
1.034MetMet: 1.034 ± 0.302
1.138MetAsn: 1.138 ± 0.222
1.758MetPro: 1.758 ± 0.355
1.81MetGln: 1.81 ± 0.288
1.861MetArg: 1.861 ± 0.262
2.327MetSer: 2.327 ± 0.377
1.396MetThr: 1.396 ± 0.253
1.448MetVal: 1.448 ± 0.275
0.569MetTrp: 0.569 ± 0.17
0.414MetTyr: 0.414 ± 0.122
0.0MetXaa: 0.0 ± 0.0
Asn
3.981AsnAla: 3.981 ± 0.428
0.259AsnCys: 0.259 ± 0.143
2.637AsnAsp: 2.637 ± 0.345
2.896AsnGlu: 2.896 ± 0.383
1.034AsnPhe: 1.034 ± 0.205
3.981AsnGly: 3.981 ± 0.633
0.62AsnHis: 0.62 ± 0.191
1.034AsnIle: 1.034 ± 0.216
1.551AsnLys: 1.551 ± 0.256
2.689AsnLeu: 2.689 ± 0.481
1.189AsnMet: 1.189 ± 0.245
1.189AsnAsn: 1.189 ± 0.285
2.327AsnPro: 2.327 ± 0.3
1.81AsnGln: 1.81 ± 0.4
1.913AsnArg: 1.913 ± 0.342
2.43AsnSer: 2.43 ± 0.351
2.689AsnThr: 2.689 ± 0.354
2.689AsnVal: 2.689 ± 0.416
0.569AsnTrp: 0.569 ± 0.231
0.879AsnTyr: 0.879 ± 0.179
0.0AsnXaa: 0.0 ± 0.0
Pro
7.911ProAla: 7.911 ± 0.945
0.31ProCys: 0.31 ± 0.144
3.619ProAsp: 3.619 ± 0.423
4.705ProGlu: 4.705 ± 0.677
1.706ProPhe: 1.706 ± 0.345
3.413ProGly: 3.413 ± 0.451
1.189ProHis: 1.189 ± 0.272
1.913ProIle: 1.913 ± 0.344
2.689ProLys: 2.689 ± 0.297
3.051ProLeu: 3.051 ± 0.38
1.086ProMet: 1.086 ± 0.246
1.603ProAsn: 1.603 ± 0.309
1.913ProPro: 1.913 ± 0.452
2.378ProGln: 2.378 ± 0.362
2.12ProArg: 2.12 ± 0.366
3.102ProSer: 3.102 ± 0.364
2.999ProThr: 2.999 ± 0.386
4.033ProVal: 4.033 ± 0.489
0.465ProTrp: 0.465 ± 0.174
0.931ProTyr: 0.931 ± 0.24
0.0ProXaa: 0.0 ± 0.0
Gln
5.222GlnAla: 5.222 ± 0.728
0.155GlnCys: 0.155 ± 0.102
2.74GlnAsp: 2.74 ± 0.468
3.257GlnGlu: 3.257 ± 0.585
1.603GlnPhe: 1.603 ± 0.367
2.585GlnGly: 2.585 ± 0.336
0.827GlnHis: 0.827 ± 0.21
1.396GlnIle: 1.396 ± 0.185
2.017GlnLys: 2.017 ± 0.291
5.739GlnLeu: 5.739 ± 0.645
1.603GlnMet: 1.603 ± 0.281
1.396GlnAsn: 1.396 ± 0.239
2.068GlnPro: 2.068 ± 0.315
4.654GlnGln: 4.654 ± 0.911
3.257GlnArg: 3.257 ± 0.35
2.999GlnSer: 2.999 ± 0.351
2.482GlnThr: 2.482 ± 0.3
1.758GlnVal: 1.758 ± 0.346
1.241GlnTrp: 1.241 ± 0.254
1.189GlnTyr: 1.189 ± 0.282
0.0GlnXaa: 0.0 ± 0.0
Arg
6.36ArgAla: 6.36 ± 0.683
0.517ArgCys: 0.517 ± 0.241
4.86ArgAsp: 4.86 ± 0.671
4.395ArgGlu: 4.395 ± 0.648
2.689ArgPhe: 2.689 ± 0.321
4.55ArgGly: 4.55 ± 0.423
1.189ArgHis: 1.189 ± 0.26
3.154ArgIle: 3.154 ± 0.395
2.947ArgLys: 2.947 ± 0.515
6.101ArgLeu: 6.101 ± 0.506
1.758ArgMet: 1.758 ± 0.236
3.051ArgAsn: 3.051 ± 0.344
3.257ArgPro: 3.257 ± 0.45
3.981ArgGln: 3.981 ± 0.428
3.826ArgArg: 3.826 ± 0.464
3.309ArgSer: 3.309 ± 0.418
3.413ArgThr: 3.413 ± 0.405
3.981ArgVal: 3.981 ± 0.375
1.241ArgTrp: 1.241 ± 0.258
2.223ArgTyr: 2.223 ± 0.363
0.0ArgXaa: 0.0 ± 0.0
Ser
6.515SerAla: 6.515 ± 0.642
0.259SerCys: 0.259 ± 0.125
3.257SerAsp: 3.257 ± 0.353
3.619SerGlu: 3.619 ± 0.512
1.396SerPhe: 1.396 ± 0.269
5.791SerGly: 5.791 ± 0.534
0.982SerHis: 0.982 ± 0.248
2.43SerIle: 2.43 ± 0.314
2.43SerLys: 2.43 ± 0.432
4.137SerLeu: 4.137 ± 0.407
1.551SerMet: 1.551 ± 0.261
1.913SerAsn: 1.913 ± 0.35
2.637SerPro: 2.637 ± 0.369
2.275SerGln: 2.275 ± 0.362
3.878SerArg: 3.878 ± 0.479
3.051SerSer: 3.051 ± 0.463
3.309SerThr: 3.309 ± 0.421
3.309SerVal: 3.309 ± 0.515
1.086SerTrp: 1.086 ± 0.277
1.344SerTyr: 1.344 ± 0.361
0.0SerXaa: 0.0 ± 0.0
Thr
7.239ThrAla: 7.239 ± 0.732
0.259ThrCys: 0.259 ± 0.131
4.033ThrAsp: 4.033 ± 0.509
3.568ThrGlu: 3.568 ± 0.447
1.913ThrPhe: 1.913 ± 0.324
4.964ThrGly: 4.964 ± 0.664
0.931ThrHis: 0.931 ± 0.265
2.482ThrIle: 2.482 ± 0.419
2.999ThrLys: 2.999 ± 0.362
3.619ThrLeu: 3.619 ± 0.472
1.241ThrMet: 1.241 ± 0.255
2.12ThrAsn: 2.12 ± 0.307
3.671ThrPro: 3.671 ± 0.41
2.43ThrGln: 2.43 ± 0.305
3.516ThrArg: 3.516 ± 0.42
3.568ThrSer: 3.568 ± 0.386
3.723ThrThr: 3.723 ± 0.414
4.55ThrVal: 4.55 ± 0.585
0.879ThrTrp: 0.879 ± 0.231
0.724ThrTyr: 0.724 ± 0.286
0.0ThrXaa: 0.0 ± 0.0
Val
7.497ValAla: 7.497 ± 0.589
0.672ValCys: 0.672 ± 0.227
3.206ValAsp: 3.206 ± 0.517
3.619ValGlu: 3.619 ± 0.423
0.931ValPhe: 0.931 ± 0.206
4.757ValGly: 4.757 ± 0.477
0.931ValHis: 0.931 ± 0.267
2.275ValIle: 2.275 ± 0.387
3.723ValLys: 3.723 ± 0.458
4.757ValLeu: 4.757 ± 0.785
1.448ValMet: 1.448 ± 0.212
2.327ValAsn: 2.327 ± 0.398
3.413ValPro: 3.413 ± 0.577
3.154ValGln: 3.154 ± 0.381
4.55ValArg: 4.55 ± 0.418
3.671ValSer: 3.671 ± 0.479
4.343ValThr: 4.343 ± 0.5
4.188ValVal: 4.188 ± 0.52
1.189ValTrp: 1.189 ± 0.249
1.499ValTyr: 1.499 ± 0.296
0.0ValXaa: 0.0 ± 0.0
Trp
1.655TrpAla: 1.655 ± 0.246
0.052TrpCys: 0.052 ± 0.05
1.138TrpAsp: 1.138 ± 0.158
0.724TrpGlu: 0.724 ± 0.21
0.776TrpPhe: 0.776 ± 0.188
1.086TrpGly: 1.086 ± 0.253
0.517TrpHis: 0.517 ± 0.171
0.724TrpIle: 0.724 ± 0.217
0.724TrpLys: 0.724 ± 0.165
1.758TrpLeu: 1.758 ± 0.31
0.362TrpMet: 0.362 ± 0.161
0.776TrpAsn: 0.776 ± 0.269
0.982TrpPro: 0.982 ± 0.261
1.086TrpGln: 1.086 ± 0.238
1.551TrpArg: 1.551 ± 0.358
1.034TrpSer: 1.034 ± 0.17
0.62TrpThr: 0.62 ± 0.253
0.931TrpVal: 0.931 ± 0.26
0.31TrpTrp: 0.31 ± 0.14
0.62TrpTyr: 0.62 ± 0.287
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.534TyrAla: 2.534 ± 0.289
0.414TyrCys: 0.414 ± 0.197
1.655TyrAsp: 1.655 ± 0.351
1.448TyrGlu: 1.448 ± 0.294
0.982TyrPhe: 0.982 ± 0.237
1.913TyrGly: 1.913 ± 0.298
0.31TyrHis: 0.31 ± 0.104
0.776TyrIle: 0.776 ± 0.182
1.086TyrLys: 1.086 ± 0.25
1.603TyrLeu: 1.603 ± 0.416
0.827TyrMet: 0.827 ± 0.186
1.034TyrAsn: 1.034 ± 0.228
1.448TyrPro: 1.448 ± 0.222
1.293TyrGln: 1.293 ± 0.189
2.275TyrArg: 2.275 ± 0.284
1.551TyrSer: 1.551 ± 0.279
1.861TyrThr: 1.861 ± 0.359
1.448TyrVal: 1.448 ± 0.387
0.465TyrTrp: 0.465 ± 0.146
0.517TyrTyr: 0.517 ± 0.215
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 64 proteins (19341 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski