Citrobacter phage Moon
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 298 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A0YP50|A0A0A0YP50_9CAUD Uncharacterized protein OS=Citrobacter phage Moon OX=1540095 GN=CPT_Moon93 PE=4 SV=1
MM1 pKa = 7.49 TKK3 pKa = 9.64 TEE5 pKa = 4.43 IVDD8 pKa = 3.78 DD9 pKa = 4.33 LQLAGYY15 pKa = 7.33 FACVKK20 pKa = 10.42 DD21 pKa = 3.59 DD22 pKa = 4.65 RR23 pKa = 11.84 IWIEE27 pKa = 3.89 GTSEE31 pKa = 4.13 YY32 pKa = 10.92 GVDD35 pKa = 3.17 WVIEE39 pKa = 4.03 EE40 pKa = 5.81 DD41 pKa = 4.37 FDD43 pKa = 4.48 AWWLYY48 pKa = 10.78 EE49 pKa = 4.05 FTGKK53 pKa = 10.28 DD54 pKa = 3.94 YY55 pKa = 11.66 YY56 pKa = 11.51 SVDD59 pKa = 2.99 AFGNMDD65 pKa = 4.33 DD66 pKa = 5.1 ALNGANKK73 pKa = 10.21 LL74 pKa = 3.8
Molecular weight: 8.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.751
IPC2_protein 3.706
IPC_protein 3.668
Toseland 3.452
ProMoST 3.859
Dawson 3.668
Bjellqvist 3.834
Wikipedia 3.643
Rodwell 3.503
Grimsley 3.376
Solomon 3.656
Lehninger 3.605
Nozaki 3.808
DTASelect 4.037
Thurlkill 3.528
EMBOSS 3.643
Sillero 3.795
Patrickios 1.863
IPC_peptide 3.643
IPC2_peptide 3.757
IPC2.peptide.svr19 3.744
Protein with the highest isoelectric point:
>tr|A0A0A0YTH8|A0A0A0YTH8_9CAUD Uncharacterized protein OS=Citrobacter phage Moon OX=1540095 GN=CPT_Moon107 PE=4 SV=1
MM1 pKa = 7.75 TITTRR6 pKa = 11.84 ITVTKK11 pKa = 9.76 EE12 pKa = 2.84 ISLYY16 pKa = 9.46 ISYY19 pKa = 10.64 GRR21 pKa = 11.84 IHH23 pKa = 7.17 PRR25 pKa = 11.84 TARR28 pKa = 11.84 RR29 pKa = 11.84 WYY31 pKa = 10.43 LEE33 pKa = 3.84 AVCRR37 pKa = 11.84 EE38 pKa = 3.67 TGRR41 pKa = 11.84 RR42 pKa = 11.84 VNAKK46 pKa = 9.68 FVVRR50 pKa = 11.84 PTKK53 pKa = 10.14 KK54 pKa = 9.98 QIRR57 pKa = 11.84 KK58 pKa = 7.45 FKK60 pKa = 9.25 RR61 pKa = 11.84 WAIEE65 pKa = 3.58 RR66 pKa = 11.84 LRR68 pKa = 11.84 FSLYY72 pKa = 9.2 WDD74 pKa = 4.55 EE75 pKa = 4.28 II76 pKa = 4.08
Molecular weight: 9.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.257
IPC2_protein 10.101
IPC_protein 11.184
Toseland 11.242
ProMoST 11.506
Dawson 11.286
Bjellqvist 11.169
Wikipedia 11.652
Rodwell 11.286
Grimsley 11.33
Solomon 11.623
Lehninger 11.55
Nozaki 11.228
DTASelect 11.155
Thurlkill 11.242
EMBOSS 11.696
Sillero 11.257
Patrickios 11.023
IPC_peptide 11.637
IPC2_peptide 10.526
IPC2.peptide.svr19 8.722
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
298
0
298
54073
33
1265
181.5
20.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.709 ± 0.157
1.097 ± 0.077
6.521 ± 0.103
7.285 ± 0.161
4.409 ± 0.106
6.145 ± 0.151
1.909 ± 0.099
7.151 ± 0.128
7.503 ± 0.185
7.471 ± 0.125
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.996 ± 0.078
5.372 ± 0.101
3.449 ± 0.088
3.427 ± 0.094
4.594 ± 0.105
6.21 ± 0.141
5.644 ± 0.195
6.475 ± 0.124
1.476 ± 0.06
4.157 ± 0.124
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here