Amino acid dipepetide frequency for Rhodococcus sp. (strain AD45)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.004AlaAla: 17.004 ± 0.118	0.894AlaCys: 0.894 ± 0.024	7.693AlaAsp: 7.693 ± 0.073	7.387AlaGlu: 7.387 ± 0.072	3.57AlaPhe: 3.57 ± 0.043	10.708AlaGly: 10.708 ± 0.079	2.467AlaHis: 2.467 ± 0.034	5.45AlaIle: 5.45 ± 0.067	3.064AlaLys: 3.064 ± 0.046	12.884AlaLeu: 12.884 ± 0.094	2.735AlaMet: 2.735 ± 0.039	2.484AlaAsn: 2.484 ± 0.035	5.641AlaPro: 5.641 ± 0.074	3.855AlaGln: 3.855 ± 0.044	7.448AlaArg: 7.448 ± 0.066	6.73AlaSer: 6.73 ± 0.052	6.921AlaThr: 6.921 ± 0.048	11.199AlaVal: 11.199 ± 0.084	1.433AlaTrp: 1.433 ± 0.03	2.21AlaTyr: 2.21 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	0.997CysAla: 0.997 ± 0.023	0.093CysCys: 0.093 ± 0.007	0.486CysAsp: 0.486 ± 0.017	0.416CysGlu: 0.416 ± 0.014	0.214CysPhe: 0.214 ± 0.01	0.886CysGly: 0.886 ± 0.023	0.18CysHis: 0.18 ± 0.011	0.3CysIle: 0.3 ± 0.012	0.133CysLys: 0.133 ± 0.008	0.559CysLeu: 0.559 ± 0.016	0.135CysMet: 0.135 ± 0.008	0.178CysAsn: 0.178 ± 0.009	0.416CysPro: 0.416 ± 0.017	0.183CysGln: 0.183 ± 0.01	0.48CysArg: 0.48 ± 0.015	0.549CysSer: 0.549 ± 0.017	0.474CysThr: 0.474 ± 0.016	0.622CysVal: 0.622 ± 0.017	0.092CysTrp: 0.092 ± 0.007	0.162CysTyr: 0.162 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.402AspAla: 7.402 ± 0.063	0.421AspCys: 0.421 ± 0.016	3.659AspAsp: 3.659 ± 0.044	3.738AspGlu: 3.738 ± 0.046	1.84AspPhe: 1.84 ± 0.031	5.755AspGly: 5.755 ± 0.062	1.371AspHis: 1.371 ± 0.028	2.926AspIle: 2.926 ± 0.04	1.449AspLys: 1.449 ± 0.03	6.294AspLeu: 6.294 ± 0.061	1.046AspMet: 1.046 ± 0.02	1.53AspAsn: 1.53 ± 0.027	4.018AspPro: 4.018 ± 0.051	1.872AspGln: 1.872 ± 0.03	4.239AspArg: 4.239 ± 0.054	3.477AspSer: 3.477 ± 0.039	3.296AspThr: 3.296 ± 0.042	5.38AspVal: 5.38 ± 0.061	0.895AspTrp: 0.895 ± 0.025	1.304AspTyr: 1.304 ± 0.025	0.0AspXaa: 0.0 ± 0.0
Glu	5.829GluAla: 5.829 ± 0.062	0.376GluCys: 0.376 ± 0.013	2.629GluAsp: 2.629 ± 0.036	2.778GluGlu: 2.778 ± 0.041	2.059GluPhe: 2.059 ± 0.038	3.661GluGly: 3.661 ± 0.043	1.482GluHis: 1.482 ± 0.024	3.082GluIle: 3.082 ± 0.042	1.731GluLys: 1.731 ± 0.032	6.887GluLeu: 6.887 ± 0.081	1.145GluMet: 1.145 ± 0.024	1.592GluAsn: 1.592 ± 0.026	2.776GluPro: 2.776 ± 0.041	2.314GluGln: 2.314 ± 0.034	4.293GluArg: 4.293 ± 0.056	3.658GluSer: 3.658 ± 0.052	2.938GluThr: 2.938 ± 0.041	4.281GluVal: 4.281 ± 0.052	0.837GluTrp: 0.837 ± 0.018	1.267GluTyr: 1.267 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	3.969PheAla: 3.969 ± 0.05	0.291PheCys: 0.291 ± 0.011	2.653PheAsp: 2.653 ± 0.046	1.81PheGlu: 1.81 ± 0.034	1.039PhePhe: 1.039 ± 0.026	3.565PheGly: 3.565 ± 0.051	0.669PheHis: 0.669 ± 0.019	1.211PheIle: 1.211 ± 0.027	0.553PheLys: 0.553 ± 0.019	2.658PheLeu: 2.658 ± 0.047	0.529PheMet: 0.529 ± 0.017	0.759PheAsn: 0.759 ± 0.02	1.485PhePro: 1.485 ± 0.022	0.755PheGln: 0.755 ± 0.024	1.697PheArg: 1.697 ± 0.031	1.974PheSer: 1.974 ± 0.036	2.205PheThr: 2.205 ± 0.032	2.838PheVal: 2.838 ± 0.041	0.424PheTrp: 0.424 ± 0.015	0.684PheTyr: 0.684 ± 0.018	0.0PheXaa: 0.0 ± 0.0
Gly	9.57GlyAla: 9.57 ± 0.076	0.727GlyCys: 0.727 ± 0.021	4.81GlyAsp: 4.81 ± 0.05	4.549GlyGlu: 4.549 ± 0.055	3.206GlyPhe: 3.206 ± 0.043	7.621GlyGly: 7.621 ± 0.078	1.842GlyHis: 1.842 ± 0.032	4.846GlyIle: 4.846 ± 0.058	2.691GlyLys: 2.691 ± 0.042	8.466GlyLeu: 8.466 ± 0.081	2.051GlyMet: 2.051 ± 0.034	2.255GlyAsn: 2.255 ± 0.034	3.957GlyPro: 3.957 ± 0.05	2.591GlyGln: 2.591 ± 0.04	5.603GlyArg: 5.603 ± 0.055	5.964GlySer: 5.964 ± 0.057	5.575GlyThr: 5.575 ± 0.056	7.53GlyVal: 7.53 ± 0.067	1.489GlyTrp: 1.489 ± 0.029	2.279GlyTyr: 2.279 ± 0.038	0.0GlyXaa: 0.0 ± 0.0
His	2.195HisAla: 2.195 ± 0.036	0.214HisCys: 0.214 ± 0.011	1.268HisAsp: 1.268 ± 0.026	1.099HisGlu: 1.099 ± 0.025	0.687HisPhe: 0.687 ± 0.017	1.956HisGly: 1.956 ± 0.035	0.676HisHis: 0.676 ± 0.02	0.913HisIle: 0.913 ± 0.021	0.385HisLys: 0.385 ± 0.012	2.066HisLeu: 2.066 ± 0.034	0.383HisMet: 0.383 ± 0.014	0.486HisAsn: 0.486 ± 0.015	1.452HisPro: 1.452 ± 0.027	0.612HisGln: 0.612 ± 0.018	1.61HisArg: 1.61 ± 0.029	1.344HisSer: 1.344 ± 0.024	1.196HisThr: 1.196 ± 0.02	1.671HisVal: 1.671 ± 0.029	0.312HisTrp: 0.312 ± 0.012	0.49HisTyr: 0.49 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	6.993IleAla: 6.993 ± 0.073	0.427IleCys: 0.427 ± 0.018	3.69IleAsp: 3.69 ± 0.044	3.037IleGlu: 3.037 ± 0.04	1.287IlePhe: 1.287 ± 0.034	4.894IleGly: 4.894 ± 0.056	0.865IleHis: 0.865 ± 0.024	1.73IleIle: 1.73 ± 0.035	0.954IleLys: 0.954 ± 0.024	4.04IleLeu: 4.04 ± 0.061	0.75IleMet: 0.75 ± 0.023	1.189IleAsn: 1.189 ± 0.024	2.637IlePro: 2.637 ± 0.037	0.951IleGln: 0.951 ± 0.022	2.825IleArg: 2.825 ± 0.035	3.048IleSer: 3.048 ± 0.046	3.1IleThr: 3.1 ± 0.043	4.353IleVal: 4.353 ± 0.051	0.522IleTrp: 0.522 ± 0.017	0.832IleTyr: 0.832 ± 0.02	0.0IleXaa: 0.0 ± 0.0
Lys	2.963LysAla: 2.963 ± 0.049	0.131LysCys: 0.131 ± 0.009	1.224LysAsp: 1.224 ± 0.027	1.127LysGlu: 1.127 ± 0.027	0.809LysPhe: 0.809 ± 0.021	1.704LysGly: 1.704 ± 0.033	0.526LysHis: 0.526 ± 0.015	1.377LysIle: 1.377 ± 0.028	0.885LysLys: 0.885 ± 0.028	2.407LysLeu: 2.407 ± 0.04	0.577LysMet: 0.577 ± 0.019	0.704LysAsn: 0.704 ± 0.019	1.447LysPro: 1.447 ± 0.031	0.74LysGln: 0.74 ± 0.02	1.567LysArg: 1.567 ± 0.029	1.595LysSer: 1.595 ± 0.029	1.526LysThr: 1.526 ± 0.032	2.101LysVal: 2.101 ± 0.034	0.356LysTrp: 0.356 ± 0.015	0.604LysTyr: 0.604 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	13.018LeuAla: 13.018 ± 0.092	0.79LeuCys: 0.79 ± 0.022	6.876LeuAsp: 6.876 ± 0.081	5.225LeuGlu: 5.225 ± 0.061	2.88LeuPhe: 2.88 ± 0.045	9.075LeuGly: 9.075 ± 0.075	1.962LeuHis: 1.962 ± 0.03	4.633LeuIle: 4.633 ± 0.061	1.832LeuLys: 1.832 ± 0.037	9.328LeuLeu: 9.328 ± 0.1	1.812LeuMet: 1.812 ± 0.03	2.153LeuAsn: 2.153 ± 0.034	5.415LeuPro: 5.415 ± 0.055	2.325LeuGln: 2.325 ± 0.038	6.944LeuArg: 6.944 ± 0.071	6.331LeuSer: 6.331 ± 0.056	6.643LeuThr: 6.643 ± 0.061	8.558LeuVal: 8.558 ± 0.074	1.166LeuTrp: 1.166 ± 0.024	1.674LeuTyr: 1.674 ± 0.031	0.0LeuXaa: 0.0 ± 0.0
Met	2.388MetAla: 2.388 ± 0.032	0.188MetCys: 0.188 ± 0.009	0.967MetAsp: 0.967 ± 0.022	0.763MetGlu: 0.763 ± 0.022	0.702MetPhe: 0.702 ± 0.02	1.501MetGly: 1.501 ± 0.029	0.427MetHis: 0.427 ± 0.016	1.107MetIle: 1.107 ± 0.027	0.565MetLys: 0.565 ± 0.019	2.072MetLeu: 2.072 ± 0.034	0.475MetMet: 0.475 ± 0.015	0.616MetAsn: 0.616 ± 0.015	1.129MetPro: 1.129 ± 0.025	0.576MetGln: 0.576 ± 0.016	1.394MetArg: 1.394 ± 0.028	1.829MetSer: 1.829 ± 0.032	1.89MetThr: 1.89 ± 0.033	1.591MetVal: 1.591 ± 0.027	0.284MetTrp: 0.284 ± 0.012	0.392MetTyr: 0.392 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	2.786AsnAla: 2.786 ± 0.036	0.206AsnCys: 0.206 ± 0.011	1.25AsnAsp: 1.25 ± 0.025	1.149AsnGlu: 1.149 ± 0.026	0.76AsnPhe: 0.76 ± 0.02	2.198AsnGly: 2.198 ± 0.036	0.48AsnHis: 0.48 ± 0.016	1.146AsnIle: 1.146 ± 0.027	0.627AsnLys: 0.627 ± 0.018	2.304AsnLeu: 2.304 ± 0.033	0.521AsnMet: 0.521 ± 0.017	0.682AsnAsn: 0.682 ± 0.023	1.851AsnPro: 1.851 ± 0.029	0.714AsnGln: 0.714 ± 0.019	1.551AsnArg: 1.551 ± 0.025	1.604AsnSer: 1.604 ± 0.031	1.555AsnThr: 1.555 ± 0.026	1.875AsnVal: 1.875 ± 0.033	0.386AsnTrp: 0.386 ± 0.014	0.587AsnTyr: 0.587 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	6.184ProAla: 6.184 ± 0.07	0.247ProCys: 0.247 ± 0.013	3.849ProAsp: 3.849 ± 0.048	3.752ProGlu: 3.752 ± 0.045	1.595ProPhe: 1.595 ± 0.03	4.713ProGly: 4.713 ± 0.058	1.111ProHis: 1.111 ± 0.023	2.39ProIle: 2.39 ± 0.033	1.371ProLys: 1.371 ± 0.03	4.673ProLeu: 4.673 ± 0.057	1.084ProMet: 1.084 ± 0.022	1.326ProAsn: 1.326 ± 0.028	2.384ProPro: 2.384 ± 0.048	1.545ProGln: 1.545 ± 0.032	2.972ProArg: 2.972 ± 0.046	3.401ProSer: 3.401 ± 0.04	3.553ProThr: 3.553 ± 0.045	4.731ProVal: 4.731 ± 0.061	0.768ProTrp: 0.768 ± 0.021	1.091ProTyr: 1.091 ± 0.024	0.0ProXaa: 0.0 ± 0.0
Gln	3.251GlnAla: 3.251 ± 0.043	0.203GlnCys: 0.203 ± 0.01	1.246GlnAsp: 1.246 ± 0.025	1.288GlnGlu: 1.288 ± 0.028	1.059GlnPhe: 1.059 ± 0.021	1.947GlnGly: 1.947 ± 0.035	0.649GlnHis: 0.649 ± 0.017	1.67GlnIle: 1.67 ± 0.028	0.74GlnLys: 0.74 ± 0.02	3.309GlnLeu: 3.309 ± 0.04	0.665GlnMet: 0.665 ± 0.019	0.703GlnAsn: 0.703 ± 0.018	1.48GlnPro: 1.48 ± 0.031	1.191GlnGln: 1.191 ± 0.033	2.168GlnArg: 2.168 ± 0.038	1.778GlnSer: 1.778 ± 0.03	1.492GlnThr: 1.492 ± 0.026	2.546GlnVal: 2.546 ± 0.044	0.575GlnTrp: 0.575 ± 0.018	0.642GlnTyr: 0.642 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	7.07ArgAla: 7.07 ± 0.066	0.474ArgCys: 0.474 ± 0.017	3.747ArgAsp: 3.747 ± 0.042	3.97ArgGlu: 3.97 ± 0.047	2.138ArgPhe: 2.138 ± 0.035	4.929ArgGly: 4.929 ± 0.058	1.461ArgHis: 1.461 ± 0.028	3.603ArgIle: 3.603 ± 0.044	1.768ArgLys: 1.768 ± 0.032	6.268ArgLeu: 6.268 ± 0.079	1.684ArgMet: 1.684 ± 0.027	1.621ArgAsn: 1.621 ± 0.027	3.085ArgPro: 3.085 ± 0.041	1.9ArgGln: 1.9 ± 0.034	5.262ArgArg: 5.262 ± 0.06	4.382ArgSer: 4.382 ± 0.055	4.407ArgThr: 4.407 ± 0.053	5.152ArgVal: 5.152 ± 0.054	1.088ArgTrp: 1.088 ± 0.024	1.643ArgTyr: 1.643 ± 0.029	0.0ArgXaa: 0.0 ± 0.0
Ser	7.94SerAla: 7.94 ± 0.064	0.422SerCys: 0.422 ± 0.014	3.885SerAsp: 3.885 ± 0.048	3.43SerGlu: 3.43 ± 0.041	1.978SerPhe: 1.978 ± 0.03	6.484SerGly: 6.484 ± 0.065	1.12SerHis: 1.12 ± 0.024	3.12SerIle: 3.12 ± 0.041	1.481SerLys: 1.481 ± 0.032	5.698SerLeu: 5.698 ± 0.062	1.565SerMet: 1.565 ± 0.026	1.417SerAsn: 1.417 ± 0.026	3.211SerPro: 3.211 ± 0.044	1.61SerGln: 1.61 ± 0.027	3.848SerArg: 3.848 ± 0.049	4.072SerSer: 4.072 ± 0.057	4.299SerThr: 4.299 ± 0.051	5.665SerVal: 5.665 ± 0.059	0.976SerTrp: 0.976 ± 0.023	1.323SerTyr: 1.323 ± 0.027	0.0SerXaa: 0.0 ± 0.0
Thr	7.775ThrAla: 7.775 ± 0.062	0.399ThrCys: 0.399 ± 0.014	3.885ThrAsp: 3.885 ± 0.048	3.554ThrGlu: 3.554 ± 0.045	1.97ThrPhe: 1.97 ± 0.033	5.774ThrGly: 5.774 ± 0.056	1.242ThrHis: 1.242 ± 0.023	2.788ThrIle: 2.788 ± 0.041	1.418ThrLys: 1.418 ± 0.032	6.008ThrLeu: 6.008 ± 0.056	1.261ThrMet: 1.261 ± 0.024	1.334ThrAsn: 1.334 ± 0.033	3.845ThrPro: 3.845 ± 0.048	1.554ThrGln: 1.554 ± 0.03	3.59ThrArg: 3.59 ± 0.045	3.992ThrSer: 3.992 ± 0.041	4.38ThrThr: 4.38 ± 0.072	6.18ThrVal: 6.18 ± 0.06	0.874ThrTrp: 0.874 ± 0.022	1.379ThrTyr: 1.379 ± 0.027	0.0ThrXaa: 0.0 ± 0.0
Val	10.747ValAla: 10.747 ± 0.077	0.696ValCys: 0.696 ± 0.02	6.07ValAsp: 6.07 ± 0.064	4.818ValGlu: 4.818 ± 0.053	2.651ValPhe: 2.651 ± 0.04	7.231ValGly: 7.231 ± 0.068	1.792ValHis: 1.792 ± 0.029	4.283ValIle: 4.283 ± 0.057	1.883ValLys: 1.883 ± 0.035	8.987ValLeu: 8.987 ± 0.075	1.723ValMet: 1.723 ± 0.034	2.2ValAsn: 2.2 ± 0.032	4.654ValPro: 4.654 ± 0.056	2.099ValGln: 2.099 ± 0.034	5.491ValArg: 5.491 ± 0.053	5.432ValSer: 5.432 ± 0.058	5.631ValThr: 5.631 ± 0.054	8.794ValVal: 8.794 ± 0.092	1.059ValTrp: 1.059 ± 0.028	1.503ValTyr: 1.503 ± 0.029	0.0ValXaa: 0.0 ± 0.0
Trp	1.453TrpAla: 1.453 ± 0.026	0.155TrpCys: 0.155 ± 0.009	0.749TrpAsp: 0.749 ± 0.02	0.632TrpGlu: 0.632 ± 0.018	0.545TrpPhe: 0.545 ± 0.015	1.027TrpGly: 1.027 ± 0.023	0.311TrpHis: 0.311 ± 0.013	0.779TrpIle: 0.779 ± 0.024	0.414TrpLys: 0.414 ± 0.015	1.485TrpLeu: 1.485 ± 0.03	0.376TrpMet: 0.376 ± 0.011	0.486TrpAsn: 0.486 ± 0.016	0.683TrpPro: 0.683 ± 0.019	0.539TrpGln: 0.539 ± 0.016	1.011TrpArg: 1.011 ± 0.025	0.999TrpSer: 0.999 ± 0.023	0.938TrpThr: 0.938 ± 0.023	1.015TrpVal: 1.015 ± 0.023	0.311TrpTrp: 0.311 ± 0.013	0.306TrpTyr: 0.306 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.241TyrAla: 2.241 ± 0.037	0.197TyrCys: 0.197 ± 0.01	1.274TyrAsp: 1.274 ± 0.029	1.096TyrGlu: 1.096 ± 0.027	0.78TyrPhe: 0.78 ± 0.019	1.992TyrGly: 1.992 ± 0.027	0.384TyrHis: 0.384 ± 0.015	0.769TyrIle: 0.769 ± 0.022	0.428TyrLys: 0.428 ± 0.015	2.247TyrLeu: 2.247 ± 0.031	0.338TyrMet: 0.338 ± 0.012	0.517TyrAsn: 0.517 ± 0.019	1.174TyrPro: 1.174 ± 0.023	0.676TyrGln: 0.676 ± 0.02	1.66TyrArg: 1.66 ± 0.028	1.385TyrSer: 1.385 ± 0.026	1.182TyrThr: 1.182 ± 0.027	1.665TyrVal: 1.665 ± 0.034	0.367TyrTrp: 0.367 ± 0.014	0.441TyrTyr: 0.441 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6266 proteins (2061266 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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