Amino acid dipepetide frequency for Sphingobium sp. RAC03

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.994AlaAla: 17.994 ± 0.179	1.113AlaCys: 1.113 ± 0.029	8.219AlaAsp: 8.219 ± 0.089	6.704AlaGlu: 6.704 ± 0.09	4.234AlaPhe: 4.234 ± 0.058	10.548AlaGly: 10.548 ± 0.109	2.433AlaHis: 2.433 ± 0.048	7.118AlaIle: 7.118 ± 0.082	4.189AlaLys: 4.189 ± 0.077	14.106AlaLeu: 14.106 ± 0.148	4.283AlaMet: 4.283 ± 0.061	3.162AlaAsn: 3.162 ± 0.055	6.34AlaPro: 6.34 ± 0.083	5.244AlaGln: 5.244 ± 0.077	9.517AlaArg: 9.517 ± 0.127	6.392AlaSer: 6.392 ± 0.088	6.753AlaThr: 6.753 ± 0.074	8.25AlaVal: 8.25 ± 0.077	1.567AlaTrp: 1.567 ± 0.034	2.679AlaTyr: 2.679 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	1.078CysAla: 1.078 ± 0.031	0.117CysCys: 0.117 ± 0.01	0.583CysAsp: 0.583 ± 0.024	0.353CysGlu: 0.353 ± 0.016	0.276CysPhe: 0.276 ± 0.015	0.858CysGly: 0.858 ± 0.03	0.215CysHis: 0.215 ± 0.013	0.395CysIle: 0.395 ± 0.019	0.192CysLys: 0.192 ± 0.013	0.705CysLeu: 0.705 ± 0.024	0.155CysMet: 0.155 ± 0.012	0.22CysAsn: 0.22 ± 0.013	0.464CysPro: 0.464 ± 0.021	0.228CysGln: 0.228 ± 0.014	0.59CysArg: 0.59 ± 0.022	0.452CysSer: 0.452 ± 0.02	0.426CysThr: 0.426 ± 0.021	0.565CysVal: 0.565 ± 0.023	0.131CysTrp: 0.131 ± 0.01	0.185CysTyr: 0.185 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	8.211AspAla: 8.211 ± 0.078	0.506AspCys: 0.506 ± 0.02	3.634AspAsp: 3.634 ± 0.071	3.164AspGlu: 3.164 ± 0.059	2.25AspPhe: 2.25 ± 0.05	6.11AspGly: 6.11 ± 0.092	1.493AspHis: 1.493 ± 0.036	3.368AspIle: 3.368 ± 0.054	1.897AspLys: 1.897 ± 0.049	6.03AspLeu: 6.03 ± 0.065	1.744AspMet: 1.744 ± 0.042	1.524AspAsn: 1.524 ± 0.033	3.769AspPro: 3.769 ± 0.057	2.072AspGln: 2.072 ± 0.041	5.033AspArg: 5.033 ± 0.079	2.597AspSer: 2.597 ± 0.046	2.701AspThr: 2.701 ± 0.059	4.18AspVal: 4.18 ± 0.061	1.158AspTrp: 1.158 ± 0.033	1.792AspTyr: 1.792 ± 0.035	0.0AspXaa: 0.0 ± 0.0
Glu	7.159GluAla: 7.159 ± 0.087	0.267GluCys: 0.267 ± 0.014	2.615GluAsp: 2.615 ± 0.05	2.763GluGlu: 2.763 ± 0.058	1.317GluPhe: 1.317 ± 0.033	4.383GluGly: 4.383 ± 0.058	0.941GluHis: 0.941 ± 0.028	2.825GluIle: 2.825 ± 0.046	1.928GluLys: 1.928 ± 0.044	4.378GluLeu: 4.378 ± 0.063	1.406GluMet: 1.406 ± 0.036	1.251GluAsn: 1.251 ± 0.03	2.341GluPro: 2.341 ± 0.043	2.169GluGln: 2.169 ± 0.045	4.383GluArg: 4.383 ± 0.069	2.175GluSer: 2.175 ± 0.037	2.981GluThr: 2.981 ± 0.055	3.119GluVal: 3.119 ± 0.057	0.724GluTrp: 0.724 ± 0.024	0.892GluTyr: 0.892 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	4.791PheAla: 4.791 ± 0.062	0.333PheCys: 0.333 ± 0.018	2.786PheAsp: 2.786 ± 0.05	1.842PheGlu: 1.842 ± 0.033	1.224PhePhe: 1.224 ± 0.035	3.45PheGly: 3.45 ± 0.065	0.758PheHis: 0.758 ± 0.023	1.467PheIle: 1.467 ± 0.037	0.864PheLys: 0.864 ± 0.027	3.036PheLeu: 3.036 ± 0.054	0.774PheMet: 0.774 ± 0.025	1.02PheAsn: 1.02 ± 0.03	1.492PhePro: 1.492 ± 0.035	0.936PheGln: 0.936 ± 0.029	2.208PheArg: 2.208 ± 0.042	2.009PheSer: 2.009 ± 0.038	2.075PheThr: 2.075 ± 0.043	2.381PheVal: 2.381 ± 0.046	0.513PheTrp: 0.513 ± 0.02	0.892PheTyr: 0.892 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	9.755GlyAla: 9.755 ± 0.118	0.823GlyCys: 0.823 ± 0.026	5.229GlyAsp: 5.229 ± 0.087	4.476GlyGlu: 4.476 ± 0.062	3.566GlyPhe: 3.566 ± 0.051	8.011GlyGly: 8.011 ± 0.146	1.897GlyHis: 1.897 ± 0.044	4.495GlyIle: 4.495 ± 0.064	3.39GlyLys: 3.39 ± 0.059	8.523GlyLeu: 8.523 ± 0.079	2.477GlyMet: 2.477 ± 0.048	2.297GlyAsn: 2.297 ± 0.083	3.561GlyPro: 3.561 ± 0.053	3.347GlyGln: 3.347 ± 0.046	6.339GlyArg: 6.339 ± 0.075	4.702GlySer: 4.702 ± 0.069	4.843GlyThr: 4.843 ± 0.081	6.233GlyVal: 6.233 ± 0.073	1.659GlyTrp: 1.659 ± 0.039	2.381GlyTyr: 2.381 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	2.347HisAla: 2.347 ± 0.048	0.243HisCys: 0.243 ± 0.013	1.312HisAsp: 1.312 ± 0.032	0.934HisGlu: 0.934 ± 0.029	0.837HisPhe: 0.837 ± 0.024	2.03HisGly: 2.03 ± 0.044	0.633HisHis: 0.633 ± 0.026	1.094HisIle: 1.094 ± 0.03	0.496HisLys: 0.496 ± 0.018	1.927HisLeu: 1.927 ± 0.047	0.535HisMet: 0.535 ± 0.021	0.461HisAsn: 0.461 ± 0.021	1.298HisPro: 1.298 ± 0.035	0.633HisGln: 0.633 ± 0.022	1.479HisArg: 1.479 ± 0.032	0.949HisSer: 0.949 ± 0.027	0.599HisThr: 0.599 ± 0.021	1.613HisVal: 1.613 ± 0.038	0.384HisTrp: 0.384 ± 0.017	0.635HisTyr: 0.635 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	8.285IleAla: 8.285 ± 0.087	0.455IleCys: 0.455 ± 0.019	4.326IleAsp: 4.326 ± 0.057	3.412IleGlu: 3.412 ± 0.055	1.763IlePhe: 1.763 ± 0.038	5.275IleGly: 5.275 ± 0.075	0.956IleHis: 0.956 ± 0.026	2.467IleIle: 2.467 ± 0.046	1.339IleLys: 1.339 ± 0.037	4.452IleLeu: 4.452 ± 0.07	1.066IleMet: 1.066 ± 0.029	1.408IleAsn: 1.408 ± 0.034	2.285IlePro: 2.285 ± 0.044	1.202IleGln: 1.202 ± 0.029	3.227IleArg: 3.227 ± 0.047	2.749IleSer: 2.749 ± 0.05	2.558IleThr: 2.558 ± 0.051	4.091IleVal: 4.091 ± 0.052	0.618IleTrp: 0.618 ± 0.022	1.013IleTyr: 1.013 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	4.291LysAla: 4.291 ± 0.069	0.171LysCys: 0.171 ± 0.011	1.742LysAsp: 1.742 ± 0.039	1.179LysGlu: 1.179 ± 0.034	0.851LysPhe: 0.851 ± 0.029	2.837LysGly: 2.837 ± 0.053	0.502LysHis: 0.502 ± 0.023	1.576LysIle: 1.576 ± 0.041	1.061LysLys: 1.061 ± 0.04	3.159LysLeu: 3.159 ± 0.051	0.813LysMet: 0.813 ± 0.025	0.72LysAsn: 0.72 ± 0.028	2.027LysPro: 2.027 ± 0.046	0.951LysGln: 0.951 ± 0.028	2.26LysArg: 2.26 ± 0.044	1.626LysSer: 1.626 ± 0.035	1.701LysThr: 1.701 ± 0.033	2.16LysVal: 2.16 ± 0.049	0.384LysTrp: 0.384 ± 0.016	0.587LysTyr: 0.587 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	13.39LeuAla: 13.39 ± 0.119	0.901LeuCys: 0.901 ± 0.028	6.147LeuAsp: 6.147 ± 0.077	4.503LeuGlu: 4.503 ± 0.06	3.723LeuPhe: 3.723 ± 0.062	7.943LeuGly: 7.943 ± 0.088	1.921LeuHis: 1.921 ± 0.046	5.214LeuIle: 5.214 ± 0.073	2.889LeuLys: 2.889 ± 0.045	9.623LeuLeu: 9.623 ± 0.135	2.353LeuMet: 2.353 ± 0.047	2.403LeuAsn: 2.403 ± 0.047	5.685LeuPro: 5.685 ± 0.071	2.505LeuGln: 2.505 ± 0.048	6.732LeuArg: 6.732 ± 0.087	6.19LeuSer: 6.19 ± 0.084	5.704LeuThr: 5.704 ± 0.071	6.728LeuVal: 6.728 ± 0.086	1.339LeuTrp: 1.339 ± 0.035	2.075LeuTyr: 2.075 ± 0.046	0.0LeuXaa: 0.0 ± 0.0
Met	3.699MetAla: 3.699 ± 0.053	0.143MetCys: 0.143 ± 0.01	1.357MetAsp: 1.357 ± 0.033	1.154MetGlu: 1.154 ± 0.033	0.739MetPhe: 0.739 ± 0.023	2.144MetGly: 2.144 ± 0.042	0.444MetHis: 0.444 ± 0.02	1.479MetIle: 1.479 ± 0.037	0.905MetLys: 0.905 ± 0.028	2.78MetLeu: 2.78 ± 0.054	0.703MetMet: 0.703 ± 0.024	0.693MetAsn: 0.693 ± 0.022	1.58MetPro: 1.58 ± 0.04	0.865MetGln: 0.865 ± 0.026	1.946MetArg: 1.946 ± 0.039	1.45MetSer: 1.45 ± 0.036	2.024MetThr: 2.024 ± 0.037	1.828MetVal: 1.828 ± 0.038	0.225MetTrp: 0.225 ± 0.011	0.271MetTyr: 0.271 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.262AsnAla: 3.262 ± 0.06	0.223AsnCys: 0.223 ± 0.013	1.584AsnAsp: 1.584 ± 0.061	1.077AsnGlu: 1.077 ± 0.035	0.94AsnPhe: 0.94 ± 0.032	2.528AsnGly: 2.528 ± 0.049	0.494AsnHis: 0.494 ± 0.02	1.426AsnIle: 1.426 ± 0.038	0.682AsnLys: 0.682 ± 0.02	2.389AsnLeu: 2.389 ± 0.055	0.609AsnMet: 0.609 ± 0.022	0.745AsnAsn: 0.745 ± 0.026	1.767AsnPro: 1.767 ± 0.037	0.801AsnGln: 0.801 ± 0.025	1.875AsnArg: 1.875 ± 0.037	1.35AsnSer: 1.35 ± 0.039	0.938AsnThr: 0.938 ± 0.032	1.824AsnVal: 1.824 ± 0.043	0.451AsnTrp: 0.451 ± 0.02	0.679AsnTyr: 0.679 ± 0.026	0.0AsnXaa: 0.0 ± 0.0
Pro	6.67ProAla: 6.67 ± 0.09	0.337ProCys: 0.337 ± 0.015	4.169ProAsp: 4.169 ± 0.055	2.977ProGlu: 2.977 ± 0.051	1.979ProPhe: 1.979 ± 0.042	4.584ProGly: 4.584 ± 0.055	1.099ProHis: 1.099 ± 0.03	2.638ProIle: 2.638 ± 0.045	1.525ProLys: 1.525 ± 0.033	4.936ProLeu: 4.936 ± 0.072	1.401ProMet: 1.401 ± 0.033	1.293ProAsn: 1.293 ± 0.037	2.853ProPro: 2.853 ± 0.069	1.824ProGln: 1.824 ± 0.033	2.921ProArg: 2.921 ± 0.059	2.769ProSer: 2.769 ± 0.046	2.869ProThr: 2.869 ± 0.047	4.428ProVal: 4.428 ± 0.066	0.71ProTrp: 0.71 ± 0.024	1.084ProTyr: 1.084 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	4.691GlnAla: 4.691 ± 0.077	0.27GlnCys: 0.27 ± 0.015	1.741GlnAsp: 1.741 ± 0.034	1.431GlnGlu: 1.431 ± 0.035	1.087GlnPhe: 1.087 ± 0.028	2.809GlnGly: 2.809 ± 0.039	0.603GlnHis: 0.603 ± 0.021	1.94GlnIle: 1.94 ± 0.039	0.989GlnLys: 0.989 ± 0.031	3.124GlnLeu: 3.124 ± 0.054	0.961GlnMet: 0.961 ± 0.031	0.797GlnAsn: 0.797 ± 0.027	1.964GlnPro: 1.964 ± 0.045	1.274GlnGln: 1.274 ± 0.036	2.674GlnArg: 2.674 ± 0.054	1.89GlnSer: 1.89 ± 0.042	1.753GlnThr: 1.753 ± 0.034	2.376GlnVal: 2.376 ± 0.045	0.54GlnTrp: 0.54 ± 0.019	0.652GlnTyr: 0.652 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	8.791ArgAla: 8.791 ± 0.104	0.542ArgCys: 0.542 ± 0.023	4.479ArgAsp: 4.479 ± 0.062	3.466ArgGlu: 3.466 ± 0.057	3.004ArgPhe: 3.004 ± 0.052	4.915ArgGly: 4.915 ± 0.063	1.756ArgHis: 1.756 ± 0.037	4.176ArgIle: 4.176 ± 0.056	2.146ArgLys: 2.146 ± 0.038	8.024ArgLeu: 8.024 ± 0.109	1.946ArgMet: 1.946 ± 0.039	1.893ArgAsn: 1.893 ± 0.047	3.64ArgPro: 3.64 ± 0.052	2.583ArgGln: 2.583 ± 0.048	5.485ArgArg: 5.485 ± 0.083	3.61ArgSer: 3.61 ± 0.049	3.594ArgThr: 3.594 ± 0.049	4.611ArgVal: 4.611 ± 0.064	1.223ArgTrp: 1.223 ± 0.028	2.025ArgTyr: 2.025 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	6.411SerAla: 6.411 ± 0.077	0.445SerCys: 0.445 ± 0.019	3.267SerAsp: 3.267 ± 0.057	2.359SerGlu: 2.359 ± 0.046	2.112SerPhe: 2.112 ± 0.045	5.321SerGly: 5.321 ± 0.065	1.005SerHis: 1.005 ± 0.027	2.818SerIle: 2.818 ± 0.05	1.498SerLys: 1.498 ± 0.039	5.177SerLeu: 5.177 ± 0.068	1.263SerMet: 1.263 ± 0.035	1.421SerAsn: 1.421 ± 0.041	2.835SerPro: 2.835 ± 0.047	1.554SerGln: 1.554 ± 0.04	3.499SerArg: 3.499 ± 0.056	2.703SerSer: 2.703 ± 0.056	2.59SerThr: 2.59 ± 0.048	3.72SerVal: 3.72 ± 0.057	0.803SerTrp: 0.803 ± 0.026	1.443SerTyr: 1.443 ± 0.031	0.0SerXaa: 0.0 ± 0.0
Thr	6.249ThrAla: 6.249 ± 0.077	0.408ThrCys: 0.408 ± 0.019	3.105ThrAsp: 3.105 ± 0.054	2.156ThrGlu: 2.156 ± 0.046	1.733ThrPhe: 1.733 ± 0.04	5.41ThrGly: 5.41 ± 0.085	1.023ThrHis: 1.023 ± 0.032	3.128ThrIle: 3.128 ± 0.056	1.421ThrLys: 1.421 ± 0.034	6.054ThrLeu: 6.054 ± 0.072	1.302ThrMet: 1.302 ± 0.035	1.322ThrAsn: 1.322 ± 0.038	3.621ThrPro: 3.621 ± 0.061	1.721ThrGln: 1.721 ± 0.037	3.479ThrArg: 3.479 ± 0.056	2.664ThrSer: 2.664 ± 0.047	2.685ThrThr: 2.685 ± 0.056	4.057ThrVal: 4.057 ± 0.059	0.584ThrTrp: 0.584 ± 0.022	1.182ThrTyr: 1.182 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	9.614ValAla: 9.614 ± 0.091	0.514ValCys: 0.514 ± 0.021	4.485ValAsp: 4.485 ± 0.06	4.397ValGlu: 4.397 ± 0.06	1.837ValPhe: 1.837 ± 0.042	5.628ValGly: 5.628 ± 0.073	1.36ValHis: 1.36 ± 0.038	3.672ValIle: 3.672 ± 0.06	2.106ValLys: 2.106 ± 0.044	5.705ValLeu: 5.705 ± 0.082	1.749ValMet: 1.749 ± 0.036	1.926ValAsn: 1.926 ± 0.043	3.764ValPro: 3.764 ± 0.057	2.274ValGln: 2.274 ± 0.041	5.025ValArg: 5.025 ± 0.065	3.828ValSer: 3.828 ± 0.059	4.562ValThr: 4.562 ± 0.061	4.942ValVal: 4.942 ± 0.076	0.806ValTrp: 0.806 ± 0.027	1.363ValTyr: 1.363 ± 0.03	0.0ValXaa: 0.0 ± 0.0
Trp	1.422TrpAla: 1.422 ± 0.037	0.121TrpCys: 0.121 ± 0.009	0.809TrpAsp: 0.809 ± 0.028	0.573TrpGlu: 0.573 ± 0.022	0.505TrpPhe: 0.505 ± 0.021	1.006TrpGly: 1.006 ± 0.029	0.367TrpHis: 0.367 ± 0.016	0.716TrpIle: 0.716 ± 0.023	0.442TrpLys: 0.442 ± 0.017	1.685TrpLeu: 1.685 ± 0.041	0.405TrpMet: 0.405 ± 0.018	0.48TrpAsn: 0.48 ± 0.02	0.731TrpPro: 0.731 ± 0.025	0.62TrpGln: 0.62 ± 0.025	1.333TrpArg: 1.333 ± 0.034	0.896TrpSer: 0.896 ± 0.029	0.923TrpThr: 0.923 ± 0.032	0.854TrpVal: 0.854 ± 0.029	0.249TrpTrp: 0.249 ± 0.013	0.288TrpTyr: 0.288 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.737TyrAla: 2.737 ± 0.049	0.255TyrCys: 0.255 ± 0.015	1.645TyrAsp: 1.645 ± 0.035	1.097TyrGlu: 1.097 ± 0.028	0.875TyrPhe: 0.875 ± 0.03	2.216TyrGly: 2.216 ± 0.051	0.539TyrHis: 0.539 ± 0.023	0.937TyrIle: 0.937 ± 0.029	0.641TyrLys: 0.641 ± 0.021	2.153TyrLeu: 2.153 ± 0.046	0.484TyrMet: 0.484 ± 0.021	0.608TyrAsn: 0.608 ± 0.028	1.046TyrPro: 1.046 ± 0.029	0.764TyrGln: 0.764 ± 0.024	1.905TyrArg: 1.905 ± 0.045	1.242TyrSer: 1.242 ± 0.036	1.024TyrThr: 1.024 ± 0.031	1.597TyrVal: 1.597 ± 0.034	0.355TyrTrp: 0.355 ± 0.016	0.652TyrTyr: 0.652 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4109 proteins (1290787 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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