Ctenophore-associated circular genome 3
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A141MJB5|A0A141MJB5_9VIRU ATP-dependent helicase Rep OS=Ctenophore-associated circular genome 3 OX=1778572 PE=3 SV=1
MM1 pKa = 7.57 VDD3 pKa = 3.12 SQGTRR8 pKa = 11.84 IVNYY12 pKa = 9.36 VFTWNNYY19 pKa = 7.47 TDD21 pKa = 3.78 DD22 pKa = 3.76 VKK24 pKa = 11.57 AFISNFANDD33 pKa = 3.33 YY34 pKa = 10.2 CKK36 pKa = 9.84 WLVYY40 pKa = 10.49 GEE42 pKa = 4.35 EE43 pKa = 4.3 VGEE46 pKa = 4.32 SGTPHH51 pKa = 6.57 LQGCLSLRR59 pKa = 11.84 VKK61 pKa = 10.64 KK62 pKa = 10.63 RR63 pKa = 11.84 ITQLRR68 pKa = 11.84 KK69 pKa = 10.08 LGFSCHH75 pKa = 6.19 LEE77 pKa = 3.98 AMKK80 pKa = 10.64 GSPKK84 pKa = 10.0 QAIAYY89 pKa = 7.33 CKK91 pKa = 10.02 KK92 pKa = 10.33 DD93 pKa = 3.35 GNVTEE98 pKa = 5.01 FGDD101 pKa = 3.52 VTRR104 pKa = 11.84 GQGEE108 pKa = 4.08 RR109 pKa = 11.84 TDD111 pKa = 3.79 LKK113 pKa = 10.71 RR114 pKa = 11.84 ACEE117 pKa = 4.27 LIKK120 pKa = 10.54 EE121 pKa = 4.38 GQGMKK126 pKa = 10.35 AVLEE130 pKa = 4.35 EE131 pKa = 4.39 CPGVYY136 pKa = 9.85 VRR138 pKa = 11.84 YY139 pKa = 10.16 SRR141 pKa = 11.84 GLRR144 pKa = 11.84 DD145 pKa = 3.33 LALAANIAYY154 pKa = 9.32 EE155 pKa = 4.11 HH156 pKa = 6.23 EE157 pKa = 4.41 TTRR160 pKa = 11.84 GIWVYY165 pKa = 10.79 GKK167 pKa = 10.27 SGVGKK172 pKa = 8.74 SHH174 pKa = 6.15 VVRR177 pKa = 11.84 ATYY180 pKa = 10.17 PDD182 pKa = 4.2 YY183 pKa = 10.92 FDD185 pKa = 5.07 KK186 pKa = 11.26 SQSKK190 pKa = 7.98 WWDD193 pKa = 3.56 GYY195 pKa = 10.17 DD196 pKa = 3.46 NEE198 pKa = 4.58 RR199 pKa = 11.84 VVVLDD204 pKa = 5.89 DD205 pKa = 5.42 LDD207 pKa = 4.36 SDD209 pKa = 4.81 CLGHH213 pKa = 6.5 YY214 pKa = 9.67 LKK216 pKa = 10.58 RR217 pKa = 11.84 WADD220 pKa = 3.47 KK221 pKa = 10.68 YY222 pKa = 10.87 SCTGEE227 pKa = 4.35 TKK229 pKa = 10.7 GGTVKK234 pKa = 10.4 LQHH237 pKa = 6.29 RR238 pKa = 11.84 VFVVTSNYY246 pKa = 10.01 LPWALHH252 pKa = 5.42 EE253 pKa = 4.24 NEE255 pKa = 4.57 MMAEE259 pKa = 4.54 AISRR263 pKa = 11.84 RR264 pKa = 11.84 FTIYY268 pKa = 10.12 HH269 pKa = 5.67 KK270 pKa = 10.64 RR271 pKa = 11.84 NRR273 pKa = 11.84 DD274 pKa = 3.36 EE275 pKa = 5.27 LLEE278 pKa = 4.19 EE279 pKa = 4.74 CEE281 pKa = 3.91 ILTYY285 pKa = 10.82 ADD287 pKa = 3.38 RR288 pKa = 11.84 HH289 pKa = 5.76 NIII292 pKa = 4.56
Molecular weight: 33.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.664
IPC2_protein 6.81
IPC_protein 6.854
Toseland 6.707
ProMoST 7.351
Dawson 7.454
Bjellqvist 7.571
Wikipedia 7.38
Rodwell 7.454
Grimsley 6.795
Solomon 7.497
Lehninger 7.512
Nozaki 7.849
DTASelect 7.658
Thurlkill 7.702
EMBOSS 7.732
Sillero 7.922
Patrickios 4.012
IPC_peptide 7.497
IPC2_peptide 7.19
IPC2.peptide.svr19 7.086
Protein with the highest isoelectric point:
>tr|A0A141MJB5|A0A141MJB5_9VIRU ATP-dependent helicase Rep OS=Ctenophore-associated circular genome 3 OX=1778572 PE=3 SV=1
MM1 pKa = 7.57 VDD3 pKa = 3.12 SQGTRR8 pKa = 11.84 IVNYY12 pKa = 9.36 VFTWNNYY19 pKa = 7.47 TDD21 pKa = 3.78 DD22 pKa = 3.76 VKK24 pKa = 11.57 AFISNFANDD33 pKa = 3.33 YY34 pKa = 10.2 CKK36 pKa = 9.84 WLVYY40 pKa = 10.49 GEE42 pKa = 4.35 EE43 pKa = 4.3 VGEE46 pKa = 4.32 SGTPHH51 pKa = 6.57 LQGCLSLRR59 pKa = 11.84 VKK61 pKa = 10.64 KK62 pKa = 10.63 RR63 pKa = 11.84 ITQLRR68 pKa = 11.84 KK69 pKa = 10.08 LGFSCHH75 pKa = 6.19 LEE77 pKa = 3.98 AMKK80 pKa = 10.64 GSPKK84 pKa = 10.0 QAIAYY89 pKa = 7.33 CKK91 pKa = 10.02 KK92 pKa = 10.33 DD93 pKa = 3.35 GNVTEE98 pKa = 5.01 FGDD101 pKa = 3.52 VTRR104 pKa = 11.84 GQGEE108 pKa = 4.08 RR109 pKa = 11.84 TDD111 pKa = 3.79 LKK113 pKa = 10.71 RR114 pKa = 11.84 ACEE117 pKa = 4.27 LIKK120 pKa = 10.54 EE121 pKa = 4.38 GQGMKK126 pKa = 10.35 AVLEE130 pKa = 4.35 EE131 pKa = 4.39 CPGVYY136 pKa = 9.85 VRR138 pKa = 11.84 YY139 pKa = 10.16 SRR141 pKa = 11.84 GLRR144 pKa = 11.84 DD145 pKa = 3.33 LALAANIAYY154 pKa = 9.32 EE155 pKa = 4.11 HH156 pKa = 6.23 EE157 pKa = 4.41 TTRR160 pKa = 11.84 GIWVYY165 pKa = 10.79 GKK167 pKa = 10.27 SGVGKK172 pKa = 8.74 SHH174 pKa = 6.15 VVRR177 pKa = 11.84 ATYY180 pKa = 10.17 PDD182 pKa = 4.2 YY183 pKa = 10.92 FDD185 pKa = 5.07 KK186 pKa = 11.26 SQSKK190 pKa = 7.98 WWDD193 pKa = 3.56 GYY195 pKa = 10.17 DD196 pKa = 3.46 NEE198 pKa = 4.58 RR199 pKa = 11.84 VVVLDD204 pKa = 5.89 DD205 pKa = 5.42 LDD207 pKa = 4.36 SDD209 pKa = 4.81 CLGHH213 pKa = 6.5 YY214 pKa = 9.67 LKK216 pKa = 10.58 RR217 pKa = 11.84 WADD220 pKa = 3.47 KK221 pKa = 10.68 YY222 pKa = 10.87 SCTGEE227 pKa = 4.35 TKK229 pKa = 10.7 GGTVKK234 pKa = 10.4 LQHH237 pKa = 6.29 RR238 pKa = 11.84 VFVVTSNYY246 pKa = 10.01 LPWALHH252 pKa = 5.42 EE253 pKa = 4.24 NEE255 pKa = 4.57 MMAEE259 pKa = 4.54 AISRR263 pKa = 11.84 RR264 pKa = 11.84 FTIYY268 pKa = 10.12 HH269 pKa = 5.67 KK270 pKa = 10.64 RR271 pKa = 11.84 NRR273 pKa = 11.84 DD274 pKa = 3.36 EE275 pKa = 5.27 LLEE278 pKa = 4.19 EE279 pKa = 4.74 CEE281 pKa = 3.91 ILTYY285 pKa = 10.82 ADD287 pKa = 3.38 RR288 pKa = 11.84 HH289 pKa = 5.76 NIII292 pKa = 4.56
Molecular weight: 33.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.664
IPC2_protein 6.81
IPC_protein 6.854
Toseland 6.707
ProMoST 7.351
Dawson 7.454
Bjellqvist 7.571
Wikipedia 7.38
Rodwell 7.454
Grimsley 6.795
Solomon 7.497
Lehninger 7.512
Nozaki 7.849
DTASelect 7.658
Thurlkill 7.702
EMBOSS 7.732
Sillero 7.922
Patrickios 4.012
IPC_peptide 7.497
IPC2_peptide 7.19
IPC2.peptide.svr19 7.086
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
292
292
292
292.0
33.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.822 ± 0.0
3.082 ± 0.0
6.507 ± 0.0
7.192 ± 0.0
2.74 ± 0.0
8.219 ± 0.0
3.082 ± 0.0
4.11 ± 0.0
7.192 ± 0.0
7.877 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.712 ± 0.0
4.11 ± 0.0
1.712 ± 0.0
2.74 ± 0.0
6.849 ± 0.0
5.137 ± 0.0
5.822 ± 0.0
7.877 ± 0.0
2.397 ± 0.0
5.822 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here