Lake Sarah-associated circular virus-9

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126GA58|A0A126GA58_9VIRU Coat protein OS=Lake Sarah-associated circular virus-9 OX=1685786 PE=4 SV=1
MM1 pKa = 7.36SRR3 pKa = 11.84QLRR6 pKa = 11.84HH7 pKa = 6.04FCFTLNNYY15 pKa = 8.59VEE17 pKa = 4.67EE18 pKa = 4.05EE19 pKa = 4.62DD20 pKa = 3.65IPRR23 pKa = 11.84LQGFFEE29 pKa = 4.22QEE31 pKa = 3.16AKK33 pKa = 10.05YY34 pKa = 9.55WIIGRR39 pKa = 11.84EE40 pKa = 4.2VGDD43 pKa = 3.42SGTPHH48 pKa = 6.05LQGYY52 pKa = 10.45ASLQRR57 pKa = 11.84RR58 pKa = 11.84HH59 pKa = 5.44TFNVVRR65 pKa = 11.84DD66 pKa = 3.95KK67 pKa = 11.38LGSRR71 pKa = 11.84CHH73 pKa = 7.04IEE75 pKa = 3.96GARR78 pKa = 11.84GSARR82 pKa = 11.84QNRR85 pKa = 11.84EE86 pKa = 3.7YY87 pKa = 10.65CSKK90 pKa = 10.22GGNYY94 pKa = 9.3IEE96 pKa = 4.91GGSINEE102 pKa = 4.23GAASGKK108 pKa = 10.3SRR110 pKa = 11.84DD111 pKa = 3.56EE112 pKa = 4.19LGRR115 pKa = 11.84SFMAAVEE122 pKa = 4.31LGNSGVVEE130 pKa = 4.19YY131 pKa = 11.22ANSFPGAYY139 pKa = 8.97IFSGSNMLRR148 pKa = 11.84NALALKK154 pKa = 10.19PPIEE158 pKa = 4.29RR159 pKa = 11.84PNIKK163 pKa = 9.63VQWIYY168 pKa = 11.46GEE170 pKa = 4.19PGVGKK175 pKa = 10.39SRR177 pKa = 11.84LAHH180 pKa = 6.04SSLPEE185 pKa = 4.14AYY187 pKa = 10.18VKK189 pKa = 10.49EE190 pKa = 4.48PRR192 pKa = 11.84TKK194 pKa = 8.11WWNGYY199 pKa = 7.28MCEE202 pKa = 4.39KK203 pKa = 10.7NVIIDD208 pKa = 3.67DD209 pKa = 4.53FGPNGIDD216 pKa = 3.32INHH219 pKa = 7.46LLRR222 pKa = 11.84WFDD225 pKa = 3.45RR226 pKa = 11.84YY227 pKa = 10.77KK228 pKa = 11.0CLVEE232 pKa = 4.4NKK234 pKa = 10.12GGMIALYY241 pKa = 10.2ADD243 pKa = 3.44NFIITSNFHH252 pKa = 6.26PDD254 pKa = 3.09EE255 pKa = 4.51VFSFAGVPNVQLPALMRR272 pKa = 11.84RR273 pKa = 11.84IEE275 pKa = 4.18LVHH278 pKa = 6.1MNN280 pKa = 3.9

Molecular weight: 31.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126GA58|A0A126GA58_9VIRU Coat protein OS=Lake Sarah-associated circular virus-9 OX=1685786 PE=4 SV=1
MM1 pKa = 7.45ARR3 pKa = 11.84YY4 pKa = 7.72NQKK7 pKa = 9.97RR8 pKa = 11.84KK9 pKa = 9.77RR10 pKa = 11.84SSGPFRR16 pKa = 11.84SFKK19 pKa = 10.19KK20 pKa = 10.17RR21 pKa = 11.84RR22 pKa = 11.84FARR25 pKa = 11.84KK26 pKa = 8.93KK27 pKa = 10.64ARR29 pKa = 11.84TSYY32 pKa = 10.26INSVNGRR39 pKa = 11.84VTSTPFFMGRR49 pKa = 11.84KK50 pKa = 7.6MRR52 pKa = 11.84PKK54 pKa = 10.17KK55 pKa = 10.35YY56 pKa = 10.54RR57 pKa = 11.84NILWNATMGSEE68 pKa = 5.16KK69 pKa = 10.99YY70 pKa = 10.36NLNNTIALTVSTPASLVNYY89 pKa = 8.38TYY91 pKa = 11.29VNQQMFVGLDD101 pKa = 3.96DD102 pKa = 3.27ITKK105 pKa = 8.22YY106 pKa = 9.66TNSNGPNPTATLGKK120 pKa = 7.96VTMRR124 pKa = 11.84GGYY127 pKa = 9.89SKK129 pKa = 10.23FTLSSKK135 pKa = 10.64DD136 pKa = 3.74DD137 pKa = 3.53EE138 pKa = 5.01VIDD141 pKa = 3.74YY142 pKa = 10.19RR143 pKa = 11.84LYY145 pKa = 10.97AIWVKK150 pKa = 10.7PNGVPPGAGALGKK163 pKa = 10.69SIDD166 pKa = 3.84IAHH169 pKa = 6.76SATDD173 pKa = 3.42TSNEE177 pKa = 3.88SVRR180 pKa = 11.84ILKK183 pKa = 9.17QWSGLLDD190 pKa = 4.0RR191 pKa = 11.84SQSASFFFRR200 pKa = 11.84AKK202 pKa = 9.95IKK204 pKa = 10.77QYY206 pKa = 9.6DD207 pKa = 3.67TTMFTAGTDD216 pKa = 3.29CMYY219 pKa = 10.42WYY221 pKa = 10.36LGVGNTIDD229 pKa = 3.36ATAVSVSYY237 pKa = 10.32IQSFNCSMTMDD248 pKa = 3.73VTAA251 pKa = 4.94

Molecular weight: 28.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

531

251

280

265.5

29.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.78 ± 0.247

1.318 ± 0.329

4.143 ± 0.402

4.52 ± 2.095

4.896 ± 0.073

8.475 ± 1.072

1.695 ± 0.817

5.65 ± 0.547

5.838 ± 1.091

6.215 ± 0.652

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.202 ± 0.493

6.968 ± 0.123

4.143 ± 0.351

2.825 ± 0.023

7.156 ± 0.242

8.286 ± 1.055

5.461 ± 2.584

5.838 ± 0.087

1.695 ± 0.064

4.896 ± 0.429
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski