Sphingomonas adhaesiva

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3413 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A4I3W8|A0A2A4I3W8_9SPHN Uncharacterized protein OS=Sphingomonas adhaesiva OX=28212 GN=COA07_15910 PE=4 SV=1
MM1 pKa = 7.47SVYY4 pKa = 10.58LYY6 pKa = 10.26LFHH9 pKa = 7.47GRR11 pKa = 11.84ATPDD15 pKa = 3.18EE16 pKa = 4.87DD17 pKa = 3.93VEE19 pKa = 4.54SWGCEE24 pKa = 3.73GPAIGPLDD32 pKa = 4.12YY33 pKa = 11.54VHH35 pKa = 6.19TTYY38 pKa = 11.03GSEE41 pKa = 4.0VKK43 pKa = 10.26IRR45 pKa = 11.84GAEE48 pKa = 4.17AILEE52 pKa = 4.2KK53 pKa = 10.62FFPDD57 pKa = 3.88TEE59 pKa = 4.14IHH61 pKa = 5.8FHH63 pKa = 7.07DD64 pKa = 5.76GYY66 pKa = 11.22GEE68 pKa = 4.07HH69 pKa = 7.51AIQLAGDD76 pKa = 4.15CLPYY80 pKa = 10.44DD81 pKa = 3.64GKK83 pKa = 11.22FYY85 pKa = 11.0GDD87 pKa = 3.3WSICTGDD94 pKa = 4.12RR95 pKa = 11.84LCSPAASRR103 pKa = 11.84VKK105 pKa = 9.88PVCDD109 pKa = 3.72DD110 pKa = 3.98CGSDD114 pKa = 3.88NVTKK118 pKa = 10.66DD119 pKa = 4.13AVAAWDD125 pKa = 4.01EE126 pKa = 4.43PEE128 pKa = 4.18QAWVLLSTYY137 pKa = 11.03DD138 pKa = 3.18STTCQDD144 pKa = 4.07CEE146 pKa = 4.41RR147 pKa = 11.84EE148 pKa = 4.02SDD150 pKa = 5.93DD151 pKa = 3.37MLEE154 pKa = 3.59WRR156 pKa = 11.84TLDD159 pKa = 3.8GAPAPDD165 pKa = 4.56PDD167 pKa = 4.95APAIPANDD175 pKa = 4.31PAPPDD180 pKa = 3.56ADD182 pKa = 3.45AAA184 pKa = 4.2

Molecular weight: 20.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A4IAD9|A0A2A4IAD9_9SPHN Glucose-1-phosphate thymidylyltransferase OS=Sphingomonas adhaesiva OX=28212 GN=rfbA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3413

0

3413

1123567

29

6951

329.2

35.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.101 ± 0.077

0.698 ± 0.013

6.292 ± 0.04

4.709 ± 0.05

3.274 ± 0.029

9.167 ± 0.082

1.957 ± 0.026

4.564 ± 0.024

2.306 ± 0.031

9.672 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.3 ± 0.021

2.248 ± 0.034

5.436 ± 0.042

2.942 ± 0.022

8.063 ± 0.062

4.533 ± 0.034

5.604 ± 0.052

7.611 ± 0.033

1.42 ± 0.022

2.105 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski