Rhizophagus irregularis
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 25730 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I1GTH8|A0A2I1GTH8_9GLOM HMG box domain-containing protein (Fragment) OS=Rhizophagus irregularis OX=588596 GN=RhiirA4_283456 PE=4 SV=1
MM1 pKa = 7.55 EE2 pKa = 6.14 FIKK5 pKa = 10.7 VDD7 pKa = 3.58 PRR9 pKa = 11.84 KK10 pKa = 10.08 VLYY13 pKa = 9.9 SQYY16 pKa = 10.77 SNRR19 pKa = 11.84 PRR21 pKa = 11.84 FRR23 pKa = 11.84 DD24 pKa = 3.47 DD25 pKa = 3.89 EE26 pKa = 4.98 LIEE29 pKa = 4.1 DD30 pKa = 4.78 TIDD33 pKa = 3.35 QLVSGEE39 pKa = 4.13 ISPKK43 pKa = 9.65 NIEE46 pKa = 4.54 HH47 pKa = 6.85 IRR49 pKa = 11.84 VCTFKK54 pKa = 11.33 GKK56 pKa = 7.98 MHH58 pKa = 6.55 SLDD61 pKa = 3.23 NRR63 pKa = 11.84 RR64 pKa = 11.84 LYY66 pKa = 11.08 AFRR69 pKa = 11.84 EE70 pKa = 4.52 AIKK73 pKa = 10.15 RR74 pKa = 11.84 GCSFRR79 pKa = 11.84 TVPAIRR85 pKa = 11.84 SYY87 pKa = 11.42 EE88 pKa = 3.85 FDD90 pKa = 3.17 EE91 pKa = 5.32 LKK93 pKa = 10.78 RR94 pKa = 11.84 KK95 pKa = 6.99 MTSPPSPDD103 pKa = 3.02 YY104 pKa = 11.3 SVIKK108 pKa = 10.11 VRR110 pKa = 11.84 RR111 pKa = 11.84 DD112 pKa = 2.84 IYY114 pKa = 10.74 IPEE117 pKa = 4.67 DD118 pKa = 4.26 LYY120 pKa = 11.62 DD121 pKa = 6.09 DD122 pKa = 6.22 DD123 pKa = 7.64 DD124 pKa = 7.6 DD125 pKa = 7.65 DD126 pKa = 7.7 DD127 pKa = 7.43 DD128 pKa = 7.59 DD129 pKa = 7.63 DD130 pKa = 7.51 DD131 pKa = 6.79 DD132 pKa = 6.79 DD133 pKa = 6.23 EE134 pKa = 6.08 YY135 pKa = 11.82 DD136 pKa = 4.14 EE137 pKa = 5.96 YY138 pKa = 11.67 DD139 pKa = 4.47 DD140 pKa = 6.05 EE141 pKa = 5.56 YY142 pKa = 11.67 DD143 pKa = 3.82 EE144 pKa = 4.53 YY145 pKa = 11.63 DD146 pKa = 3.36 EE147 pKa = 5.51 YY148 pKa = 11.51 EE149 pKa = 4.7 EE150 pKa = 5.12 EE151 pKa = 6.17 DD152 pKa = 3.84 EE153 pKa = 6.24 DD154 pKa = 6.69 DD155 pKa = 4.39 DD156 pKa = 6.47 DD157 pKa = 5.92 EE158 pKa = 6.75 YY159 pKa = 11.75 DD160 pKa = 4.41 DD161 pKa = 6.03 EE162 pKa = 6.17 SDD164 pKa = 3.78 EE165 pKa = 6.34 DD166 pKa = 3.7 EE167 pKa = 5.21 DD168 pKa = 5.28 EE169 pKa = 4.44 YY170 pKa = 11.81 DD171 pKa = 3.51 EE172 pKa = 5.31 EE173 pKa = 5.42 YY174 pKa = 11.1 YY175 pKa = 10.95 GYY177 pKa = 10.73 DD178 pKa = 4.15 YY179 pKa = 11.52 DD180 pKa = 6.45 DD181 pKa = 5.21 EE182 pKa = 7.57 DD183 pKa = 6.09 DD184 pKa = 5.89 DD185 pKa = 6.09 EE186 pKa = 6.77 YY187 pKa = 11.77 DD188 pKa = 3.56 DD189 pKa = 6.13 DD190 pKa = 6.17 EE191 pKa = 6.07 YY192 pKa = 11.78 DD193 pKa = 3.03 EE194 pKa = 5.51 YY195 pKa = 11.84 YY196 pKa = 10.71 DD197 pKa = 4.4 YY198 pKa = 11.32 DD199 pKa = 3.48 ILNSFGRR206 pKa = 11.84 LYY208 pKa = 10.53 II209 pKa = 4.05
Molecular weight: 25.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.684
IPC2_protein 3.681
IPC_protein 3.719
Toseland 3.49
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.656
Rodwell 3.541
Grimsley 3.401
Solomon 3.706
Lehninger 3.668
Nozaki 3.821
DTASelect 4.088
Thurlkill 3.541
EMBOSS 3.668
Sillero 3.846
Patrickios 1.38
IPC_peptide 3.706
IPC2_peptide 3.821
IPC2.peptide.svr19 3.748
Protein with the highest isoelectric point:
>tr|A0A2I1G0Q6|A0A2I1G0Q6_9GLOM Uncharacterized protein OS=Rhizophagus irregularis OX=588596 GN=RhiirA4_453545 PE=4 SV=1
LL1 pKa = 6.51 NQRR4 pKa = 11.84 ILNQRR9 pKa = 11.84 VLNQRR14 pKa = 11.84 ILQRR18 pKa = 11.84 ILNQIFLQRR27 pKa = 11.84 ILNQIFLQRR36 pKa = 11.84 VLNQIFLQRR45 pKa = 11.84 ILNQRR50 pKa = 11.84 FLQRR54 pKa = 11.84 ILNQRR59 pKa = 11.84 FLQRR63 pKa = 11.84 ILNQIFLQRR72 pKa = 11.84 ILNQRR77 pKa = 11.84 FLQRR81 pKa = 11.84 ILNQRR86 pKa = 11.84 FLQRR90 pKa = 11.84 ILNQIFLQRR99 pKa = 11.84 ILNQRR104 pKa = 11.84 FLQRR108 pKa = 11.84 ILNQRR113 pKa = 11.84 FLQRR117 pKa = 11.84 ILNQIFLQRR126 pKa = 11.84 ILNQRR131 pKa = 11.84 FLQRR135 pKa = 11.84 ILNQRR140 pKa = 11.84 FLQRR144 pKa = 11.84 ILNQRR149 pKa = 11.84 AVLLIPRR156 pKa = 11.84 MEE158 pKa = 4.08 NRR160 pKa = 11.84 VTQMILYY167 pKa = 8.21 SKK169 pKa = 10.67 CC170 pKa = 3.2
Molecular weight: 21.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.47
IPC2_protein 11.111
IPC_protein 12.632
Toseland 12.793
ProMoST 13.29
Dawson 12.793
Bjellqvist 12.793
Wikipedia 13.276
Rodwell 12.31
Grimsley 12.837
Solomon 13.29
Lehninger 13.203
Nozaki 12.793
DTASelect 12.793
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.032
IPC_peptide 13.305
IPC2_peptide 12.296
IPC2.peptide.svr19 9.16
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
25730
0
25730
8183250
49
5487
318.0
36.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.413 ± 0.013
1.546 ± 0.008
5.829 ± 0.012
6.976 ± 0.017
4.691 ± 0.012
4.457 ± 0.013
2.17 ± 0.008
8.148 ± 0.017
7.978 ± 0.022
9.072 ± 0.018
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.975 ± 0.005
7.117 ± 0.016
4.078 ± 0.016
3.771 ± 0.012
4.456 ± 0.013
7.85 ± 0.018
5.347 ± 0.011
5.002 ± 0.012
1.174 ± 0.005
3.947 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here