Caulobacter phage Kronos
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A386KQ00|A0A386KQ00_9CAUD Uncharacterized protein OS=Caulobacter phage Kronos OX=2340873 PE=4 SV=1
MM1 pKa = 7.6 AKK3 pKa = 10.01 KK4 pKa = 9.44 EE5 pKa = 4.14 AQTQTVTIGGTVYY18 pKa = 10.41 PVRR21 pKa = 11.84 FDD23 pKa = 3.69 YY24 pKa = 11.34 GSLIAFCDD32 pKa = 3.78 EE33 pKa = 3.86 VGATIDD39 pKa = 3.52 TMQQALAALPLNRR52 pKa = 11.84 MSLVVWAGIVDD63 pKa = 3.79 WTYY66 pKa = 10.41 EE67 pKa = 3.95 TDD69 pKa = 3.84 DD70 pKa = 5.39 DD71 pKa = 4.3 EE72 pKa = 5.0 TGTPSMTPARR82 pKa = 11.84 VSRR85 pKa = 11.84 LLKK88 pKa = 10.97 GEE90 pKa = 4.37 TIEE93 pKa = 4.72 DD94 pKa = 3.52 AQAILAKK101 pKa = 10.59 CLDD104 pKa = 3.8 AFTASMAPKK113 pKa = 9.66 RR114 pKa = 11.84 KK115 pKa = 9.45 EE116 pKa = 3.59 PAEE119 pKa = 4.43 GDD121 pKa = 3.84 TASPPATPAMTSPGSTDD138 pKa = 2.74 SS139 pKa = 3.53
Molecular weight: 14.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.459
IPC2_protein 4.342
IPC_protein 4.253
Toseland 4.062
ProMoST 4.393
Dawson 4.228
Bjellqvist 4.38
Wikipedia 4.139
Rodwell 4.075
Grimsley 3.973
Solomon 4.215
Lehninger 4.164
Nozaki 4.329
DTASelect 4.546
Thurlkill 4.088
EMBOSS 4.151
Sillero 4.368
Patrickios 3.935
IPC_peptide 4.215
IPC2_peptide 4.342
IPC2.peptide.svr19 4.302
Protein with the highest isoelectric point:
>tr|A0A386KT89|A0A386KT89_9CAUD Uncharacterized protein OS=Caulobacter phage Kronos OX=2340873 PE=4 SV=1
MM1 pKa = 7.62 PGWNGTCKK9 pKa = 10.34 RR10 pKa = 11.84 YY11 pKa = 10.1 LFQVGTKK18 pKa = 10.19 CAGLPEE24 pKa = 4.79 AFQSGTKK31 pKa = 10.82 ANMEE35 pKa = 4.19 QKK37 pKa = 10.82 APAQPPRR44 pKa = 11.84 RR45 pKa = 11.84 HH46 pKa = 6.09 LSLIVKK52 pKa = 10.34 DD53 pKa = 4.1 EE54 pKa = 4.27 TQTPAARR61 pKa = 11.84 FAACLEE67 pKa = 4.51 AYY69 pKa = 10.58 SLTDD73 pKa = 3.73 DD74 pKa = 4.07 EE75 pKa = 5.21 AAALLGVSRR84 pKa = 11.84 PVVARR89 pKa = 11.84 LRR91 pKa = 11.84 LARR94 pKa = 11.84 RR95 pKa = 11.84 PASQDD100 pKa = 2.35 IAARR104 pKa = 11.84 AEE106 pKa = 4.04 LLFGIPAAHH115 pKa = 7.23 WALLPDD121 pKa = 3.58 EE122 pKa = 4.34 TVARR126 pKa = 11.84 IKK128 pKa = 10.88 ANRR131 pKa = 11.84 SALEE135 pKa = 3.56 RR136 pKa = 11.84 TARR139 pKa = 11.84 QRR141 pKa = 11.84 PTRR144 pKa = 11.84 KK145 pKa = 9.49 AAA147 pKa = 3.45
Molecular weight: 16.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 9.385
IPC_protein 9.78
Toseland 10.511
ProMoST 10.101
Dawson 10.599
Bjellqvist 10.277
Wikipedia 10.76
Rodwell 10.862
Grimsley 10.628
Solomon 10.687
Lehninger 10.672
Nozaki 10.511
DTASelect 10.248
Thurlkill 10.496
EMBOSS 10.906
Sillero 10.526
Patrickios 10.613
IPC_peptide 10.701
IPC2_peptide 9.297
IPC2.peptide.svr19 8.49
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
58
0
58
13475
51
1339
232.3
24.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.308 ± 0.556
0.994 ± 0.161
5.447 ± 0.17
5.521 ± 0.407
3.221 ± 0.147
8.757 ± 0.402
1.922 ± 0.216
3.391 ± 0.242
4.215 ± 0.402
8.364 ± 0.312
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.709 ± 0.218
2.894 ± 0.214
5.981 ± 0.363
3.555 ± 0.14
6.516 ± 0.485
4.757 ± 0.238
6.412 ± 0.415
7.391 ± 0.37
1.707 ± 0.117
1.937 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here