Heliothis virescens ascovirus 3e (HvAV-3e)
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 178 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A4KXF0|A4KXF0_HVAVE NAD-glutamate dehydrogenase OS=Heliothis virescens ascovirus 3e OX=260797 PE=4 SV=1
MM1 pKa = 7.47 NEE3 pKa = 3.51 AEE5 pKa = 5.13 LRR7 pKa = 11.84 VQYY10 pKa = 10.42 EE11 pKa = 4.27 SEE13 pKa = 4.51 DD14 pKa = 4.03 EE15 pKa = 4.52 ITLEE19 pKa = 3.87 WLKK22 pKa = 9.85 TLGVNDD28 pKa = 4.23 ADD30 pKa = 3.62 EE31 pKa = 5.47 CVAQCHH37 pKa = 5.32 VSHH40 pKa = 6.38 EE41 pKa = 4.14 QALARR46 pKa = 11.84 IKK48 pKa = 10.67 RR49 pKa = 11.84 AVDD52 pKa = 3.18 EE53 pKa = 5.46 CDD55 pKa = 3.52 DD56 pKa = 3.63 QSVIDD61 pKa = 4.2 NEE63 pKa = 4.49 VNRR66 pKa = 11.84 PPSADD71 pKa = 3.16 NVSYY75 pKa = 8.99 TAEE78 pKa = 3.96 EE79 pKa = 4.68 VYY81 pKa = 10.86 DD82 pKa = 4.01 AVSLIRR88 pKa = 11.84 DD89 pKa = 3.8 RR90 pKa = 11.84 GRR92 pKa = 11.84 MVNITNNYY100 pKa = 9.1 NFFGSPPRR108 pKa = 11.84 PSLVPPPPPPPPPYY122 pKa = 10.0 EE123 pKa = 3.82 QQ124 pKa = 3.68
Molecular weight: 13.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.268
IPC2_protein 4.304
IPC_protein 4.215
Toseland 4.05
ProMoST 4.342
Dawson 4.177
Bjellqvist 4.329
Wikipedia 4.05
Rodwell 4.05
Grimsley 3.961
Solomon 4.164
Lehninger 4.126
Nozaki 4.279
DTASelect 4.444
Thurlkill 4.062
EMBOSS 4.075
Sillero 4.329
Patrickios 3.35
IPC_peptide 4.164
IPC2_peptide 4.317
IPC2.peptide.svr19 4.237
Protein with the highest isoelectric point:
>tr|A4KXB7|A4KXB7_HVAVE Putative nucleic acid-binding protein OS=Heliothis virescens ascovirus 3e OX=260797 PE=4 SV=1
MM1 pKa = 7.73 ASRR4 pKa = 11.84 RR5 pKa = 11.84 KK6 pKa = 9.66 PSRR9 pKa = 11.84 LTAAQCEE16 pKa = 4.29 TFIRR20 pKa = 11.84 NNKK23 pKa = 8.58 AVSPLTNKK31 pKa = 10.24 PIDD34 pKa = 3.75 VYY36 pKa = 11.32 GRR38 pKa = 11.84 AAARR42 pKa = 11.84 FRR44 pKa = 11.84 RR45 pKa = 11.84 DD46 pKa = 3.7 CNLSPPPTKK55 pKa = 9.91 YY56 pKa = 9.46 TSTVCKK62 pKa = 10.3 KK63 pKa = 9.77 FLANKK68 pKa = 9.71 SVSPYY73 pKa = 9.63 SGRR76 pKa = 11.84 PIKK79 pKa = 10.58 RR80 pKa = 11.84 GAKK83 pKa = 9.77 LYY85 pKa = 11.04 NDD87 pKa = 3.81 LTKK90 pKa = 10.35 HH91 pKa = 5.73 CSGTRR96 pKa = 11.84 SSSPARR102 pKa = 11.84 SPARR106 pKa = 11.84 RR107 pKa = 11.84 RR108 pKa = 11.84 RR109 pKa = 11.84 VIRR112 pKa = 11.84 SPSPNRR118 pKa = 11.84 RR119 pKa = 11.84 SSSPRR124 pKa = 11.84 RR125 pKa = 11.84 SASPQRR131 pKa = 11.84 RR132 pKa = 11.84 RR133 pKa = 11.84 ASPQRR138 pKa = 11.84 RR139 pKa = 11.84 RR140 pKa = 11.84 ASPQRR145 pKa = 11.84 RR146 pKa = 11.84 RR147 pKa = 11.84 ASPDD151 pKa = 2.58 RR152 pKa = 11.84 SKK154 pKa = 10.64 PAKK157 pKa = 8.89 RR158 pKa = 11.84 TAANADD164 pKa = 3.61 TRR166 pKa = 11.84 PDD168 pKa = 3.15 LCATFSRR175 pKa = 11.84 NEE177 pKa = 4.28 SINPITGKK185 pKa = 10.6 KK186 pKa = 10.32 LIGTSPIRR194 pKa = 11.84 KK195 pKa = 8.42 AWHH198 pKa = 6.02 RR199 pKa = 11.84 MCAGTPNTRR208 pKa = 11.84 ATKK211 pKa = 10.57 CMAFDD216 pKa = 4.94 KK217 pKa = 10.78 NDD219 pKa = 3.44 KK220 pKa = 10.4 KK221 pKa = 11.18 NPFTGRR227 pKa = 11.84 SIRR230 pKa = 11.84 PEE232 pKa = 3.65 QPAYY236 pKa = 10.69 RR237 pKa = 11.84 MVYY240 pKa = 9.71 SMCHH244 pKa = 4.51 GVPYY248 pKa = 10.35 RR249 pKa = 11.84 SPKK252 pKa = 7.71 RR253 pKa = 11.84 TRR255 pKa = 11.84 RR256 pKa = 11.84 SPVRR260 pKa = 11.84 RR261 pKa = 11.84 SPSPRR266 pKa = 11.84 PYY268 pKa = 9.42 TATSVTRR275 pKa = 11.84 KK276 pKa = 8.58 YY277 pKa = 10.96 RR278 pKa = 11.84 RR279 pKa = 11.84 IKK281 pKa = 8.95 TPARR285 pKa = 11.84 SRR287 pKa = 11.84 SRR289 pKa = 11.84 SRR291 pKa = 11.84 SNSVGRR297 pKa = 11.84 RR298 pKa = 11.84 RR299 pKa = 11.84 TTAVKK304 pKa = 10.56 SRR306 pKa = 11.84 TKK308 pKa = 10.71 SPARR312 pKa = 11.84 RR313 pKa = 11.84 QSVARR318 pKa = 11.84 SRR320 pKa = 11.84 SRR322 pKa = 11.84 SKK324 pKa = 10.99 SPVRR328 pKa = 11.84 KK329 pKa = 5.63 TTRR332 pKa = 11.84 SRR334 pKa = 11.84 TKK336 pKa = 10.25 SPARR340 pKa = 11.84 RR341 pKa = 11.84 QSVARR346 pKa = 11.84 SRR348 pKa = 11.84 SRR350 pKa = 11.84 TKK352 pKa = 10.66 SPARR356 pKa = 11.84 RR357 pKa = 11.84 QSVARR362 pKa = 11.84 SRR364 pKa = 11.84 SRR366 pKa = 11.84 SKK368 pKa = 10.93 SPARR372 pKa = 11.84 RR373 pKa = 11.84 QSVARR378 pKa = 11.84 SRR380 pKa = 11.84 SRR382 pKa = 11.84 SKK384 pKa = 10.93 SPARR388 pKa = 11.84 RR389 pKa = 11.84 QSVAKK394 pKa = 10.05 SRR396 pKa = 11.84 SRR398 pKa = 11.84 SKK400 pKa = 10.95 SPARR404 pKa = 11.84 RR405 pKa = 11.84 QSVAMSRR412 pKa = 11.84 SRR414 pKa = 11.84 SRR416 pKa = 11.84 SRR418 pKa = 11.84 SRR420 pKa = 11.84 QPMTAMRR427 pKa = 11.84 RR428 pKa = 11.84 STSRR432 pKa = 11.84 ARR434 pKa = 11.84 SRR436 pKa = 11.84 SKK438 pKa = 10.59 SRR440 pKa = 11.84 SRR442 pKa = 11.84 KK443 pKa = 9.7 AMTASRR449 pKa = 11.84 SRR451 pKa = 11.84 SRR453 pKa = 11.84 SVSRR457 pKa = 11.84 YY458 pKa = 7.72 MINPNFVPTTTKK470 pKa = 10.14 KK471 pKa = 10.55 KK472 pKa = 9.82 RR473 pKa = 11.84 VSPVSRR479 pKa = 11.84 GRR481 pKa = 11.84 INNSRR486 pKa = 11.84 SRR488 pKa = 11.84 SASRR492 pKa = 11.84 ARR494 pKa = 11.84 SGGLSPYY501 pKa = 9.82 RR502 pKa = 11.84 GRR504 pKa = 11.84 VLLSPIPDD512 pKa = 3.73 GATPMSRR519 pKa = 11.84 SQLINIANNMNIAEE533 pKa = 4.24 LRR535 pKa = 11.84 HH536 pKa = 5.35 IVVSNGFQPVRR547 pKa = 11.84 VAQNTTQSQLLNIVKK562 pKa = 9.21 FQIRR566 pKa = 11.84 EE567 pKa = 4.25 GNLKK571 pKa = 9.24 WLPRR575 pKa = 11.84 NDD577 pKa = 3.38 QDD579 pKa = 3.62 VPTYY583 pKa = 8.27 YY584 pKa = 10.27 TSSRR588 pKa = 11.84 PFADD592 pKa = 3.04 RR593 pKa = 11.84 MKK595 pKa = 11.04 KK596 pKa = 9.43 NN597 pKa = 3.25
Molecular weight: 67.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.278
IPC2_protein 10.76
IPC_protein 12.164
Toseland 12.325
ProMoST 12.808
Dawson 12.325
Bjellqvist 12.31
Wikipedia 12.793
Rodwell 11.989
Grimsley 12.369
Solomon 12.822
Lehninger 12.72
Nozaki 12.325
DTASelect 12.31
Thurlkill 12.325
EMBOSS 12.822
Sillero 12.325
Patrickios 11.696
IPC_peptide 12.822
IPC2_peptide 11.813
IPC2.peptide.svr19 9.125
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
178
0
178
53577
53
1387
301.0
34.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.381 ± 0.162
2.684 ± 0.125
6.594 ± 0.126
5.168 ± 0.154
3.438 ± 0.116
4.789 ± 0.16
2.486 ± 0.086
5.417 ± 0.112
5.222 ± 0.155
8.27 ± 0.165
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.83 ± 0.06
5.411 ± 0.124
3.733 ± 0.123
2.815 ± 0.12
7.294 ± 0.227
8.031 ± 0.188
6.581 ± 0.136
7.813 ± 0.135
0.98 ± 0.067
4.061 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here