Amino acid dipepetide frequency for Escherichia phage Pollock

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.446AlaAla: 6.446 ± 0.941
0.525AlaCys: 0.525 ± 0.184
4.488AlaAsp: 4.488 ± 0.48
4.87AlaGlu: 4.87 ± 0.771
3.533AlaPhe: 3.533 ± 0.369
4.87AlaGly: 4.87 ± 0.444
0.955AlaHis: 0.955 ± 0.239
5.252AlaIle: 5.252 ± 0.58
5.205AlaLys: 5.205 ± 0.572
7.258AlaLeu: 7.258 ± 0.416
1.576AlaMet: 1.576 ± 0.242
4.011AlaAsn: 4.011 ± 0.662
1.767AlaPro: 1.767 ± 0.265
3.486AlaGln: 3.486 ± 0.599
3.008AlaArg: 3.008 ± 0.519
4.297AlaSer: 4.297 ± 0.398
4.154AlaThr: 4.154 ± 0.495
5.061AlaVal: 5.061 ± 0.76
0.812AlaTrp: 0.812 ± 0.191
3.199AlaTyr: 3.199 ± 0.378
0.0AlaXaa: 0.0 ± 0.0
Cys
0.477CysAla: 0.477 ± 0.154
0.286CysCys: 0.286 ± 0.134
0.525CysAsp: 0.525 ± 0.164
0.43CysGlu: 0.43 ± 0.149
0.43CysPhe: 0.43 ± 0.162
0.525CysGly: 0.525 ± 0.194
0.286CysHis: 0.286 ± 0.127
1.241CysIle: 1.241 ± 0.331
0.716CysLys: 0.716 ± 0.216
1.098CysLeu: 1.098 ± 0.315
0.143CysMet: 0.143 ± 0.093
0.382CysAsn: 0.382 ± 0.144
0.525CysPro: 0.525 ± 0.196
0.143CysGln: 0.143 ± 0.106
0.286CysArg: 0.286 ± 0.126
0.525CysSer: 0.525 ± 0.176
1.098CysThr: 1.098 ± 0.258
0.525CysVal: 0.525 ± 0.18
0.048CysTrp: 0.048 ± 0.051
0.334CysTyr: 0.334 ± 0.147
0.0CysXaa: 0.0 ± 0.0
Asp
4.727AspAla: 4.727 ± 0.596
0.477AspCys: 0.477 ± 0.153
3.056AspAsp: 3.056 ± 0.514
4.345AspGlu: 4.345 ± 0.567
1.623AspPhe: 1.623 ± 0.272
3.915AspGly: 3.915 ± 0.512
0.716AspHis: 0.716 ± 0.147
4.059AspIle: 4.059 ± 0.492
4.345AspLys: 4.345 ± 0.41
5.634AspLeu: 5.634 ± 0.451
1.289AspMet: 1.289 ± 0.191
2.722AspAsn: 2.722 ± 0.399
1.814AspPro: 1.814 ± 0.297
1.003AspGln: 1.003 ± 0.232
2.005AspArg: 2.005 ± 0.24
3.295AspSer: 3.295 ± 0.323
4.059AspThr: 4.059 ± 0.451
3.056AspVal: 3.056 ± 0.362
0.716AspTrp: 0.716 ± 0.208
2.817AspTyr: 2.817 ± 0.462
0.0AspXaa: 0.0 ± 0.0
Glu
5.539GluAla: 5.539 ± 1.09
0.668GluCys: 0.668 ± 0.232
3.008GluAsp: 3.008 ± 0.452
6.398GluGlu: 6.398 ± 0.708
2.674GluPhe: 2.674 ± 0.34
3.39GluGly: 3.39 ± 0.357
1.003GluHis: 1.003 ± 0.212
5.539GluIle: 5.539 ± 0.44
4.202GluLys: 4.202 ± 0.458
7.592GluLeu: 7.592 ± 0.654
2.149GluMet: 2.149 ± 0.339
3.056GluAsn: 3.056 ± 0.444
2.817GluPro: 2.817 ± 0.411
3.772GluGln: 3.772 ± 0.611
2.817GluArg: 2.817 ± 0.388
4.441GluSer: 4.441 ± 0.552
3.82GluThr: 3.82 ± 0.446
4.488GluVal: 4.488 ± 0.575
1.003GluTrp: 1.003 ± 0.242
2.292GluTyr: 2.292 ± 0.317
0.0GluXaa: 0.0 ± 0.0
Phe
2.34PheAla: 2.34 ± 0.318
0.525PheCys: 0.525 ± 0.182
2.005PheAsp: 2.005 ± 0.252
2.244PheGlu: 2.244 ± 0.347
0.907PhePhe: 0.907 ± 0.271
3.056PheGly: 3.056 ± 0.36
0.43PheHis: 0.43 ± 0.175
2.769PheIle: 2.769 ± 0.405
2.769PheLys: 2.769 ± 0.366
3.104PheLeu: 3.104 ± 0.375
0.955PheMet: 0.955 ± 0.229
2.531PheAsn: 2.531 ± 0.368
1.003PhePro: 1.003 ± 0.245
1.337PheGln: 1.337 ± 0.297
1.814PheArg: 1.814 ± 0.246
2.244PheSer: 2.244 ± 0.321
2.769PheThr: 2.769 ± 0.295
2.244PheVal: 2.244 ± 0.292
0.43PheTrp: 0.43 ± 0.153
1.623PheTyr: 1.623 ± 0.294
0.0PheXaa: 0.0 ± 0.0
Gly
4.345GlyAla: 4.345 ± 0.561
0.43GlyCys: 0.43 ± 0.181
3.056GlyAsp: 3.056 ± 0.409
3.677GlyGlu: 3.677 ± 0.531
2.292GlyPhe: 2.292 ± 0.277
2.626GlyGly: 2.626 ± 0.437
0.907GlyHis: 0.907 ± 0.185
4.918GlyIle: 4.918 ± 0.435
4.345GlyLys: 4.345 ± 0.422
5.157GlyLeu: 5.157 ± 0.562
1.623GlyMet: 1.623 ± 0.284
4.297GlyAsn: 4.297 ± 0.44
0.621GlyPro: 0.621 ± 0.156
2.292GlyGln: 2.292 ± 0.44
2.913GlyArg: 2.913 ± 0.426
4.154GlySer: 4.154 ± 0.574
4.488GlyThr: 4.488 ± 0.575
3.868GlyVal: 3.868 ± 0.476
0.859GlyTrp: 0.859 ± 0.218
2.674GlyTyr: 2.674 ± 0.365
0.0GlyXaa: 0.0 ± 0.0
His
0.859HisAla: 0.859 ± 0.173
0.621HisCys: 0.621 ± 0.194
1.098HisAsp: 1.098 ± 0.317
1.146HisGlu: 1.146 ± 0.255
0.621HisPhe: 0.621 ± 0.193
0.764HisGly: 0.764 ± 0.161
0.43HisHis: 0.43 ± 0.156
1.194HisIle: 1.194 ± 0.259
1.337HisLys: 1.337 ± 0.367
1.146HisLeu: 1.146 ± 0.189
0.334HisMet: 0.334 ± 0.137
0.716HisAsn: 0.716 ± 0.159
1.337HisPro: 1.337 ± 0.245
0.812HisGln: 0.812 ± 0.165
0.812HisArg: 0.812 ± 0.249
0.668HisSer: 0.668 ± 0.166
0.955HisThr: 0.955 ± 0.201
1.003HisVal: 1.003 ± 0.21
0.191HisTrp: 0.191 ± 0.095
0.764HisTyr: 0.764 ± 0.191
0.0HisXaa: 0.0 ± 0.0
Ile
6.398IleAla: 6.398 ± 0.673
0.764IleCys: 0.764 ± 0.223
4.727IleAsp: 4.727 ± 0.406
5.3IleGlu: 5.3 ± 0.45
2.626IlePhe: 2.626 ± 0.343
4.154IleGly: 4.154 ± 0.475
1.337IleHis: 1.337 ± 0.234
4.393IleIle: 4.393 ± 0.701
5.014IleLys: 5.014 ± 0.408
4.918IleLeu: 4.918 ± 0.566
1.432IleMet: 1.432 ± 0.259
4.679IleAsn: 4.679 ± 0.506
3.295IlePro: 3.295 ± 0.422
3.056IleGln: 3.056 ± 0.35
3.151IleArg: 3.151 ± 0.399
4.966IleSer: 4.966 ± 0.336
4.775IleThr: 4.775 ± 0.478
3.724IleVal: 3.724 ± 0.628
0.716IleTrp: 0.716 ± 0.153
2.34IleTyr: 2.34 ± 0.411
0.0IleXaa: 0.0 ± 0.0
Lys
5.873LysAla: 5.873 ± 0.584
0.239LysCys: 0.239 ± 0.113
3.581LysAsp: 3.581 ± 0.493
5.778LysGlu: 5.778 ± 0.581
2.387LysPhe: 2.387 ± 0.446
3.295LysGly: 3.295 ± 0.365
1.337LysHis: 1.337 ± 0.291
4.011LysIle: 4.011 ± 0.456
3.677LysLys: 3.677 ± 0.566
6.207LysLeu: 6.207 ± 0.649
2.244LysMet: 2.244 ± 0.36
3.629LysAsn: 3.629 ± 0.422
2.913LysPro: 2.913 ± 0.459
3.151LysGln: 3.151 ± 0.468
2.817LysArg: 2.817 ± 0.287
3.963LysSer: 3.963 ± 0.459
4.059LysThr: 4.059 ± 0.454
4.727LysVal: 4.727 ± 0.471
0.573LysTrp: 0.573 ± 0.161
2.531LysTyr: 2.531 ± 0.322
0.0LysXaa: 0.0 ± 0.0
Leu
6.924LeuAla: 6.924 ± 0.825
1.194LeuCys: 1.194 ± 0.299
5.061LeuAsp: 5.061 ± 0.446
5.205LeuGlu: 5.205 ± 0.505
2.817LeuPhe: 2.817 ± 0.326
5.061LeuGly: 5.061 ± 0.51
1.432LeuHis: 1.432 ± 0.275
6.112LeuIle: 6.112 ± 0.617
5.682LeuLys: 5.682 ± 0.595
7.258LeuLeu: 7.258 ± 0.682
2.435LeuMet: 2.435 ± 0.319
5.969LeuAsn: 5.969 ± 0.547
4.154LeuPro: 4.154 ± 0.518
3.39LeuGln: 3.39 ± 0.351
4.727LeuArg: 4.727 ± 0.516
6.303LeuSer: 6.303 ± 0.499
5.3LeuThr: 5.3 ± 0.434
5.205LeuVal: 5.205 ± 0.439
0.764LeuTrp: 0.764 ± 0.223
2.913LeuTyr: 2.913 ± 0.479
0.0LeuXaa: 0.0 ± 0.0
Met
1.767MetAla: 1.767 ± 0.247
0.286MetCys: 0.286 ± 0.113
1.719MetAsp: 1.719 ± 0.233
1.671MetGlu: 1.671 ± 0.291
0.716MetPhe: 0.716 ± 0.175
1.432MetGly: 1.432 ± 0.248
0.477MetHis: 0.477 ± 0.146
1.385MetIle: 1.385 ± 0.263
1.623MetLys: 1.623 ± 0.321
2.817MetLeu: 2.817 ± 0.387
0.621MetMet: 0.621 ± 0.171
1.767MetAsn: 1.767 ± 0.281
0.859MetPro: 0.859 ± 0.201
1.098MetGln: 1.098 ± 0.228
0.955MetArg: 0.955 ± 0.209
2.435MetSer: 2.435 ± 0.322
1.767MetThr: 1.767 ± 0.311
1.623MetVal: 1.623 ± 0.32
0.143MetTrp: 0.143 ± 0.093
1.003MetTyr: 1.003 ± 0.266
0.0MetXaa: 0.0 ± 0.0
Asn
4.488AsnAla: 4.488 ± 0.519
0.43AsnCys: 0.43 ± 0.164
2.196AsnAsp: 2.196 ± 0.296
4.393AsnGlu: 4.393 ± 0.438
1.767AsnPhe: 1.767 ± 0.268
4.297AsnGly: 4.297 ± 0.472
1.289AsnHis: 1.289 ± 0.227
4.154AsnIle: 4.154 ± 0.52
4.011AsnLys: 4.011 ± 0.443
4.87AsnLeu: 4.87 ± 0.436
1.528AsnMet: 1.528 ± 0.273
3.868AsnAsn: 3.868 ± 0.361
3.82AsnPro: 3.82 ± 0.543
2.674AsnGln: 2.674 ± 0.384
2.483AsnArg: 2.483 ± 0.366
3.629AsnSer: 3.629 ± 0.278
3.581AsnThr: 3.581 ± 0.468
3.724AsnVal: 3.724 ± 0.282
0.382AsnTrp: 0.382 ± 0.146
1.91AsnTyr: 1.91 ± 0.357
0.0AsnXaa: 0.0 ± 0.0
Pro
2.149ProAla: 2.149 ± 0.341
0.43ProCys: 0.43 ± 0.146
2.817ProAsp: 2.817 ± 0.408
3.772ProGlu: 3.772 ± 0.377
1.194ProPhe: 1.194 ± 0.3
2.292ProGly: 2.292 ± 0.401
0.477ProHis: 0.477 ± 0.131
2.626ProIle: 2.626 ± 0.319
2.435ProLys: 2.435 ± 0.472
2.483ProLeu: 2.483 ± 0.313
0.955ProMet: 0.955 ± 0.186
2.817ProAsn: 2.817 ± 0.35
0.764ProPro: 0.764 ± 0.236
1.194ProGln: 1.194 ± 0.277
1.241ProArg: 1.241 ± 0.223
2.722ProSer: 2.722 ± 0.314
3.247ProThr: 3.247 ± 0.415
3.199ProVal: 3.199 ± 0.377
0.334ProTrp: 0.334 ± 0.164
1.194ProTyr: 1.194 ± 0.25
0.0ProXaa: 0.0 ± 0.0
Gln
3.629GlnAla: 3.629 ± 0.576
0.239GlnCys: 0.239 ± 0.114
1.91GlnAsp: 1.91 ± 0.349
3.056GlnGlu: 3.056 ± 0.376
1.432GlnPhe: 1.432 ± 0.225
2.101GlnGly: 2.101 ± 0.38
0.668GlnHis: 0.668 ± 0.2
2.722GlnIle: 2.722 ± 0.32
3.151GlnLys: 3.151 ± 0.433
4.727GlnLeu: 4.727 ± 0.618
1.528GlnMet: 1.528 ± 0.275
2.531GlnAsn: 2.531 ± 0.408
1.194GlnPro: 1.194 ± 0.243
2.435GlnGln: 2.435 ± 0.62
1.767GlnArg: 1.767 ± 0.279
2.531GlnSer: 2.531 ± 0.484
2.34GlnThr: 2.34 ± 0.34
2.292GlnVal: 2.292 ± 0.315
0.477GlnTrp: 0.477 ± 0.132
2.34GlnTyr: 2.34 ± 0.385
0.0GlnXaa: 0.0 ± 0.0
Arg
3.247ArgAla: 3.247 ± 0.353
0.477ArgCys: 0.477 ± 0.195
2.674ArgAsp: 2.674 ± 0.397
2.483ArgGlu: 2.483 ± 0.561
1.862ArgPhe: 1.862 ± 0.311
2.149ArgGly: 2.149 ± 0.297
0.621ArgHis: 0.621 ± 0.207
3.724ArgIle: 3.724 ± 0.41
2.913ArgLys: 2.913 ± 0.349
4.154ArgLeu: 4.154 ± 0.397
1.194ArgMet: 1.194 ± 0.233
2.34ArgAsn: 2.34 ± 0.49
1.098ArgPro: 1.098 ± 0.276
2.149ArgGln: 2.149 ± 0.474
1.671ArgArg: 1.671 ± 0.28
1.91ArgSer: 1.91 ± 0.334
2.674ArgThr: 2.674 ± 0.382
2.435ArgVal: 2.435 ± 0.319
0.716ArgTrp: 0.716 ± 0.27
1.576ArgTyr: 1.576 ± 0.263
0.0ArgXaa: 0.0 ± 0.0
Ser
4.297SerAla: 4.297 ± 0.517
0.668SerCys: 0.668 ± 0.223
3.342SerAsp: 3.342 ± 0.499
4.202SerGlu: 4.202 ± 0.513
2.483SerPhe: 2.483 ± 0.339
4.727SerGly: 4.727 ± 0.547
0.764SerHis: 0.764 ± 0.205
4.345SerIle: 4.345 ± 0.456
4.011SerLys: 4.011 ± 0.459
5.969SerLeu: 5.969 ± 0.479
1.767SerMet: 1.767 ± 0.31
4.679SerAsn: 4.679 ± 0.5
2.817SerPro: 2.817 ± 0.433
3.342SerGln: 3.342 ± 0.458
2.531SerArg: 2.531 ± 0.565
3.868SerSer: 3.868 ± 0.613
3.868SerThr: 3.868 ± 0.447
4.25SerVal: 4.25 ± 0.463
0.621SerTrp: 0.621 ± 0.185
1.767SerTyr: 1.767 ± 0.335
0.0SerXaa: 0.0 ± 0.0
Thr
4.106ThrAla: 4.106 ± 0.424
0.812ThrCys: 0.812 ± 0.228
3.486ThrAsp: 3.486 ± 0.408
3.772ThrGlu: 3.772 ± 0.5
2.34ThrPhe: 2.34 ± 0.42
4.345ThrGly: 4.345 ± 0.461
1.098ThrHis: 1.098 ± 0.214
4.584ThrIle: 4.584 ± 0.666
3.963ThrLys: 3.963 ± 0.392
4.393ThrLeu: 4.393 ± 0.36
1.098ThrMet: 1.098 ± 0.253
3.295ThrAsn: 3.295 ± 0.446
3.295ThrPro: 3.295 ± 0.457
3.104ThrGln: 3.104 ± 0.374
2.483ThrArg: 2.483 ± 0.337
4.011ThrSer: 4.011 ± 0.453
3.724ThrThr: 3.724 ± 0.691
5.348ThrVal: 5.348 ± 0.361
0.764ThrTrp: 0.764 ± 0.183
2.531ThrTyr: 2.531 ± 0.434
0.0ThrXaa: 0.0 ± 0.0
Val
4.297ValAla: 4.297 ± 0.463
0.334ValCys: 0.334 ± 0.162
3.915ValAsp: 3.915 ± 0.396
4.202ValGlu: 4.202 ± 0.423
2.817ValPhe: 2.817 ± 0.48
3.915ValGly: 3.915 ± 0.406
1.528ValHis: 1.528 ± 0.289
4.536ValIle: 4.536 ± 0.443
4.536ValLys: 4.536 ± 0.474
4.775ValLeu: 4.775 ± 0.374
2.005ValMet: 2.005 ± 0.366
3.486ValAsn: 3.486 ± 0.457
2.626ValPro: 2.626 ± 0.368
2.435ValGln: 2.435 ± 0.293
2.96ValArg: 2.96 ± 0.409
4.87ValSer: 4.87 ± 0.43
3.677ValThr: 3.677 ± 0.431
4.202ValVal: 4.202 ± 0.575
0.573ValTrp: 0.573 ± 0.183
2.34ValTyr: 2.34 ± 0.363
0.0ValXaa: 0.0 ± 0.0
Trp
0.812TrpAla: 0.812 ± 0.203
0.191TrpCys: 0.191 ± 0.094
0.859TrpAsp: 0.859 ± 0.214
0.668TrpGlu: 0.668 ± 0.152
0.716TrpPhe: 0.716 ± 0.214
0.621TrpGly: 0.621 ± 0.17
0.191TrpHis: 0.191 ± 0.117
0.955TrpIle: 0.955 ± 0.182
0.764TrpLys: 0.764 ± 0.192
1.098TrpLeu: 1.098 ± 0.302
0.143TrpMet: 0.143 ± 0.081
0.525TrpAsn: 0.525 ± 0.147
0.286TrpPro: 0.286 ± 0.109
0.239TrpGln: 0.239 ± 0.105
0.43TrpArg: 0.43 ± 0.154
0.43TrpSer: 0.43 ± 0.162
0.43TrpThr: 0.43 ± 0.129
0.716TrpVal: 0.716 ± 0.201
0.191TrpTrp: 0.191 ± 0.099
0.477TrpTyr: 0.477 ± 0.161
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.053TyrAla: 2.053 ± 0.357
0.43TyrCys: 0.43 ± 0.197
2.149TyrAsp: 2.149 ± 0.436
3.056TyrGlu: 3.056 ± 0.344
1.862TyrPhe: 1.862 ± 0.256
2.005TyrGly: 2.005 ± 0.311
0.859TyrHis: 0.859 ± 0.261
3.247TyrIle: 3.247 ± 0.398
2.292TyrLys: 2.292 ± 0.301
3.247TyrLeu: 3.247 ± 0.359
1.098TyrMet: 1.098 ± 0.255
2.196TyrAsn: 2.196 ± 0.366
1.337TyrPro: 1.337 ± 0.328
2.005TyrGln: 2.005 ± 0.323
1.194TyrArg: 1.194 ± 0.247
3.056TyrSer: 3.056 ± 0.382
1.767TyrThr: 1.767 ± 0.259
2.34TyrVal: 2.34 ± 0.324
0.43TyrTrp: 0.43 ± 0.142
1.337TyrTyr: 1.337 ± 0.282
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 84 proteins (20944 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski