Opisthorchis viverrini
Average proteome isoelectric point is 7.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 16307 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A075A5H0|A0A075A5H0_9TREM Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_02786 PE=4 SV=1
MM1 pKa = 7.07 VAVCGVSPSMVVRR14 pKa = 11.84 QPNVSLFEE22 pKa = 4.23 GSQNLDD28 pKa = 2.98 VGTVVIDD35 pKa = 3.62 EE36 pKa = 4.85 RR37 pKa = 11.84 CFTWEE42 pKa = 3.78 GQSRR46 pKa = 11.84 QFSVPYY52 pKa = 9.78 QQITLHH58 pKa = 6.88 AISKK62 pKa = 9.42 DD63 pKa = 3.34 PVMAAGDD70 pKa = 3.67 QPAPEE75 pKa = 4.04 NTFPHH80 pKa = 6.17 PHH82 pKa = 6.74 LLVMIDD88 pKa = 3.25 GDD90 pKa = 3.99 RR91 pKa = 11.84 PYY93 pKa = 11.17 SPPHH97 pKa = 6.5 PNGQIAGEE105 pKa = 3.97 PMAIDD110 pKa = 3.98 GAEE113 pKa = 4.06 HH114 pKa = 7.22 SDD116 pKa = 3.21 TDD118 pKa = 3.96 GSNEE122 pKa = 4.08 SQEE125 pKa = 4.67 DD126 pKa = 3.51 ATSQGRR132 pKa = 11.84 ASDD135 pKa = 4.22 CPGEE139 pKa = 4.2 TTILRR144 pKa = 11.84 FVPQDD149 pKa = 3.54 PNALDD154 pKa = 3.26 TMYY157 pKa = 11.18 AALAEE162 pKa = 4.34 CQALNPDD169 pKa = 3.64 STDD172 pKa = 3.17 NSSTSDD178 pKa = 3.63 VEE180 pKa = 5.0 DD181 pKa = 5.55 DD182 pKa = 4.53 GDD184 pKa = 4.43 DD185 pKa = 3.8 FCFLPDD191 pKa = 4.1 IDD193 pKa = 5.69 SLDD196 pKa = 3.57 SDD198 pKa = 4.59 QYY200 pKa = 12.01 ADD202 pKa = 3.99 DD203 pKa = 3.87
Molecular weight: 21.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.688
IPC2_protein 3.668
IPC_protein 3.694
Toseland 3.465
ProMoST 3.834
Dawson 3.694
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.516
Grimsley 3.363
Solomon 3.681
Lehninger 3.643
Nozaki 3.808
DTASelect 4.088
Thurlkill 3.528
EMBOSS 3.656
Sillero 3.821
Patrickios 1.1
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.745
Protein with the highest isoelectric point:
>tr|A0A074ZIQ9|A0A074ZIQ9_9TREM Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_05731 PE=4 SV=1
KKK2 pKa = 10.54 LTRR5 pKa = 11.84 LLKKK9 pKa = 9.5 LRR11 pKa = 11.84 QPTTGCTLPLRR22 pKa = 11.84 VHHH25 pKa = 6.24 LLNDDD30 pKa = 3.33 HH31 pKa = 6.71 SSFKKK36 pKa = 10.01 QRR38 pKa = 11.84 IFNPMISRR46 pKa = 11.84 NLVLRR51 pKa = 11.84 RR52 pKa = 11.84 RR53 pKa = 11.84 FRR55 pKa = 11.84 KKK57 pKa = 8.49 IGPRR61 pKa = 11.84 QFTRR65 pKa = 11.84 FAGRR69 pKa = 11.84 SPIAVSPVTGRR80 pKa = 11.84 PLVSARR86 pKa = 11.84
Molecular weight: 10.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.41
IPC2_protein 10.877
IPC_protein 12.471
Toseland 12.647
ProMoST 13.144
Dawson 12.647
Bjellqvist 12.632
Wikipedia 13.115
Rodwell 12.252
Grimsley 12.676
Solomon 13.144
Lehninger 13.042
Nozaki 12.647
DTASelect 12.632
Thurlkill 12.647
EMBOSS 13.144
Sillero 12.647
Patrickios 11.974
IPC_peptide 13.144
IPC2_peptide 12.135
IPC2.peptide.svr19 9.122
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
16307
0
16307
7070076
30
10941
433.6
48.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.774 ± 0.016
2.182 ± 0.012
5.143 ± 0.022
5.729 ± 0.021
3.764 ± 0.014
5.554 ± 0.022
2.81 ± 0.009
4.619 ± 0.013
4.457 ± 0.019
9.814 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.041 ± 0.008
4.086 ± 0.011
6.018 ± 0.021
4.328 ± 0.012
6.664 ± 0.016
9.394 ± 0.033
6.424 ± 0.019
6.36 ± 0.016
1.207 ± 0.006
2.621 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here