Maize white line mosaic Satellite virus (MWLMV) (Satellite maize white line mosaic virus)
Average proteome isoelectric point is 9.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P29151|COAT_MWLMV Coat protein OS=Maize white line mosaic Satellite virus OX=31503 PE=4 SV=1
MM1 pKa = 7.33 ATQLTTRR8 pKa = 11.84 ARR10 pKa = 11.84 RR11 pKa = 11.84 ATRR14 pKa = 11.84 VSRR17 pKa = 11.84 KK18 pKa = 9.53 GSQPASKK25 pKa = 10.36 QDD27 pKa = 3.21 VKK29 pKa = 11.23 QVVKK33 pKa = 10.84 SILGQSLEE41 pKa = 4.14 HH42 pKa = 6.68 KK43 pKa = 9.94 RR44 pKa = 11.84 ANLLLPPTVVNTTGNIYY61 pKa = 10.64 CLTQFVIEE69 pKa = 4.6 GDD71 pKa = 4.14 GISQRR76 pKa = 11.84 TGRR79 pKa = 11.84 VINLEE84 pKa = 3.93 QMVLRR89 pKa = 11.84 YY90 pKa = 9.57 RR91 pKa = 11.84 RR92 pKa = 11.84 TLDD95 pKa = 3.17 TTSANSGFLRR105 pKa = 11.84 YY106 pKa = 9.19 IVFLDD111 pKa = 3.63 TQNQGTLPAITDD123 pKa = 3.69 VLSSLDD129 pKa = 3.38 VSSGYY134 pKa = 10.2 EE135 pKa = 3.74 VLNAQQNRR143 pKa = 11.84 FKK145 pKa = 11.03 FLLDD149 pKa = 3.65 EE150 pKa = 4.45 VEE152 pKa = 4.53 SLCASATNLSKK163 pKa = 11.2 ASTLTFNQKK172 pKa = 9.22 VQVHH176 pKa = 5.56 YY177 pKa = 11.06 GGAADD182 pKa = 3.86 AATSNRR188 pKa = 11.84 RR189 pKa = 11.84 NAVFFLEE196 pKa = 5.03 LSDD199 pKa = 4.58 KK200 pKa = 10.94 VATGPQTRR208 pKa = 11.84 LGVQLKK214 pKa = 8.18 FTDD217 pKa = 3.34 AA218 pKa = 3.92
Molecular weight: 23.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.142
IPC2_protein 9.194
IPC_protein 9.282
Toseland 9.955
ProMoST 9.648
Dawson 10.16
Bjellqvist 9.838
Wikipedia 10.321
Rodwell 10.496
Grimsley 10.233
Solomon 10.204
Lehninger 10.175
Nozaki 9.984
DTASelect 9.823
Thurlkill 10.014
EMBOSS 10.365
Sillero 10.087
Patrickios 10.101
IPC_peptide 10.204
IPC2_peptide 8.536
IPC2.peptide.svr19 8.133
Protein with the highest isoelectric point:
>sp|P29151|COAT_MWLMV Coat protein OS=Maize white line mosaic Satellite virus OX=31503 PE=4 SV=1
MM1 pKa = 7.33 ATQLTTRR8 pKa = 11.84 ARR10 pKa = 11.84 RR11 pKa = 11.84 ATRR14 pKa = 11.84 VSRR17 pKa = 11.84 KK18 pKa = 9.53 GSQPASKK25 pKa = 10.36 QDD27 pKa = 3.21 VKK29 pKa = 11.23 QVVKK33 pKa = 10.84 SILGQSLEE41 pKa = 4.14 HH42 pKa = 6.68 KK43 pKa = 9.94 RR44 pKa = 11.84 ANLLLPPTVVNTTGNIYY61 pKa = 10.64 CLTQFVIEE69 pKa = 4.6 GDD71 pKa = 4.14 GISQRR76 pKa = 11.84 TGRR79 pKa = 11.84 VINLEE84 pKa = 3.93 QMVLRR89 pKa = 11.84 YY90 pKa = 9.57 RR91 pKa = 11.84 RR92 pKa = 11.84 TLDD95 pKa = 3.17 TTSANSGFLRR105 pKa = 11.84 YY106 pKa = 9.19 IVFLDD111 pKa = 3.63 TQNQGTLPAITDD123 pKa = 3.69 VLSSLDD129 pKa = 3.38 VSSGYY134 pKa = 10.2 EE135 pKa = 3.74 VLNAQQNRR143 pKa = 11.84 FKK145 pKa = 11.03 FLLDD149 pKa = 3.65 EE150 pKa = 4.45 VEE152 pKa = 4.53 SLCASATNLSKK163 pKa = 11.2 ASTLTFNQKK172 pKa = 9.22 VQVHH176 pKa = 5.56 YY177 pKa = 11.06 GGAADD182 pKa = 3.86 AATSNRR188 pKa = 11.84 RR189 pKa = 11.84 NAVFFLEE196 pKa = 5.03 LSDD199 pKa = 4.58 KK200 pKa = 10.94 VATGPQTRR208 pKa = 11.84 LGVQLKK214 pKa = 8.18 FTDD217 pKa = 3.34 AA218 pKa = 3.92
Molecular weight: 23.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.142
IPC2_protein 9.194
IPC_protein 9.282
Toseland 9.955
ProMoST 9.648
Dawson 10.16
Bjellqvist 9.838
Wikipedia 10.321
Rodwell 10.496
Grimsley 10.233
Solomon 10.204
Lehninger 10.175
Nozaki 9.984
DTASelect 9.823
Thurlkill 10.014
EMBOSS 10.365
Sillero 10.087
Patrickios 10.101
IPC_peptide 10.204
IPC2_peptide 8.536
IPC2.peptide.svr19 8.133
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
218
218
218
218.0
23.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.257 ± 0.0
0.917 ± 0.0
4.587 ± 0.0
3.211 ± 0.0
4.128 ± 0.0
5.963 ± 0.0
0.917 ± 0.0
3.211 ± 0.0
4.587 ± 0.0
11.468 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
0.917 ± 0.0
5.505 ± 0.0
2.294 ± 0.0
7.339 ± 0.0
7.339 ± 0.0
8.257 ± 0.0
10.092 ± 0.0
8.716 ± 0.0
0.0 ± 0.0
2.294 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here