Hubei toti-like virus 15

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KFF5|A0A1L3KFF5_9VIRU Uncharacterized protein OS=Hubei toti-like virus 15 OX=1923303 PE=4 SV=1
MM1 pKa = 7.9ADD3 pKa = 3.08ATTPSVSPLTEE14 pKa = 4.02PQAEE18 pKa = 4.2NPTTSSRR25 pKa = 11.84EE26 pKa = 3.76DD27 pKa = 3.1GFASVRR33 pKa = 11.84NLQSWIKK40 pKa = 8.97TPLSIVGSNNAPLSAYY56 pKa = 7.28PTGFDD61 pKa = 3.21TASIDD66 pKa = 3.82GQKK69 pKa = 9.5KK70 pKa = 6.9QQYY73 pKa = 8.23STFSVLGASYY83 pKa = 11.02LSYY86 pKa = 11.17LCLRR90 pKa = 11.84YY91 pKa = 9.92SVISSLQQNTQTQTQGEE108 pKa = 4.4YY109 pKa = 10.66DD110 pKa = 3.69IVNPPPGAVAALTIAQQAAYY130 pKa = 9.67HH131 pKa = 5.82NKK133 pKa = 8.85EE134 pKa = 3.81LLYY137 pKa = 10.11TRR139 pKa = 11.84AHH141 pKa = 6.26AFVPTAAIARR151 pKa = 11.84KK152 pKa = 6.65MARR155 pKa = 11.84IIKK158 pKa = 8.27MGPATLSIPTVQSLQTTTPIDD179 pKa = 3.65LSEE182 pKa = 4.14QNIPTEE188 pKa = 4.28TNNAVAASIGTTSSSTTLRR207 pKa = 11.84SSSSTTSANSGPSDD221 pKa = 3.43HH222 pKa = 6.68ATGRR226 pKa = 11.84SINPQPDD233 pKa = 3.67DD234 pKa = 4.37PPCEE238 pKa = 4.62IISKK242 pKa = 10.25PEE244 pKa = 3.76VIPYY248 pKa = 9.84RR249 pKa = 11.84VVKK252 pKa = 9.76TSHH255 pKa = 6.71LAGSSEE261 pKa = 4.37VTSIPYY267 pKa = 9.85SEE269 pKa = 4.85IYY271 pKa = 10.21NAISASLSSEE281 pKa = 3.69ANTAIRR287 pKa = 11.84ATLKK291 pKa = 10.75SALVCEE297 pKa = 4.34ASTWSVLVKK306 pKa = 9.93TLSQEE311 pKa = 4.09SGSVCDD317 pKa = 3.43VAPLVRR323 pKa = 11.84LATLSYY329 pKa = 10.82ALSSTAASFDD339 pKa = 3.39TMPYY343 pKa = 10.53KK344 pKa = 10.79LFRR347 pKa = 11.84NMSVSYY353 pKa = 10.42QGTAIHH359 pKa = 6.91APDD362 pKa = 4.77PNLPHH367 pKa = 7.36SIIAMPLEE375 pKa = 4.23VFTTFMVGTAMTSPLPSIFLPSEE398 pKa = 3.85VDD400 pKa = 3.45KK401 pKa = 11.19GWVGIPISEE410 pKa = 3.9QDD412 pKa = 3.33ARR414 pKa = 11.84LPCIRR419 pKa = 11.84EE420 pKa = 4.13IITSHH425 pKa = 6.65ISSDD429 pKa = 3.57LTFGTITKK437 pKa = 9.25RR438 pKa = 11.84YY439 pKa = 8.27NVSAPGDD446 pKa = 3.32ITGQLLCNPASNMSYY461 pKa = 9.01VHH463 pKa = 6.65GPRR466 pKa = 11.84HH467 pKa = 6.03VILIIKK473 pKa = 10.06DD474 pKa = 3.24RR475 pKa = 11.84GSTNEE480 pKa = 3.94TDD482 pKa = 3.81SIQIGGLQVPVYY494 pKa = 10.52RR495 pKa = 11.84NRR497 pKa = 11.84TGQAPMITPANWTTEE512 pKa = 3.33WVRR515 pKa = 11.84YY516 pKa = 9.84YY517 pKa = 10.12NTANSAKK524 pKa = 9.96ILSNIQNAWNHH535 pKa = 5.41LTSKK539 pKa = 9.95TITLDD544 pKa = 3.26TAGRR548 pKa = 11.84ALSVAAEE555 pKa = 4.31LTLATPPGLYY565 pKa = 10.13LHH567 pKa = 7.02PNSNGIAYY575 pKa = 9.9DD576 pKa = 3.43YY577 pKa = 11.08DD578 pKa = 4.4RR579 pKa = 11.84PLSGAFVIHH588 pKa = 6.23VRR590 pKa = 11.84DD591 pKa = 3.7NNGTCKK597 pKa = 10.17LDD599 pKa = 3.42KK600 pKa = 9.58TDD602 pKa = 3.66YY603 pKa = 8.77MADD606 pKa = 3.69VITLTTPGSDD616 pKa = 3.2QTVKK620 pKa = 10.98KK621 pKa = 10.7VMAGINIASISPLHH635 pKa = 6.35LPPSGLVPTTYY646 pKa = 9.94TSSGPSVYY654 pKa = 10.53AANDD658 pKa = 3.59PDD660 pKa = 3.85LTKK663 pKa = 11.06ASDD666 pKa = 3.86TYY668 pKa = 10.85HH669 pKa = 6.62IPCSSTLKK677 pKa = 10.51RR678 pKa = 11.84LSVYY682 pKa = 10.51LGLINRR688 pKa = 11.84TSTPYY693 pKa = 10.62YY694 pKa = 9.62FASAEE699 pKa = 4.02ALSGWVHH706 pKa = 6.15MLAHH710 pKa = 6.75ANSANTAAMLQEE722 pKa = 3.95IDD724 pKa = 3.5MPARR728 pKa = 11.84DD729 pKa = 3.37WTGFNTDD736 pKa = 2.24WSEE739 pKa = 3.86NNRR742 pKa = 11.84FEE744 pKa = 5.29EE745 pKa = 4.18INSLKK750 pKa = 9.8RR751 pKa = 11.84TVSMDD756 pKa = 3.45MIKK759 pKa = 10.41HH760 pKa = 5.81FEE762 pKa = 4.13AEE764 pKa = 4.36SEE766 pKa = 4.27FEE768 pKa = 4.23SWDD771 pKa = 3.53SYY773 pKa = 11.83DD774 pKa = 4.27PDD776 pKa = 4.58WISTAYY782 pKa = 10.46GGIDD786 pKa = 3.34PFSNTMWWQQSPVPSHH802 pKa = 7.44FLLQWYY808 pKa = 8.78KK809 pKa = 10.58KK810 pKa = 9.68LKK812 pKa = 9.43TVSCPEE818 pKa = 3.9TRR820 pKa = 11.84PGQNPLLYY828 pKa = 10.98VNGRR832 pKa = 11.84KK833 pKa = 9.38KK834 pKa = 10.45FAVTMTISNKK844 pKa = 9.77PSWEE848 pKa = 4.03WIIASIDD855 pKa = 3.2THH857 pKa = 6.16HH858 pKa = 6.95RR859 pKa = 11.84HH860 pKa = 6.51PIVVDD865 pKa = 3.05TSYY868 pKa = 11.1RR869 pKa = 11.84QNRR872 pKa = 11.84ANAVLGSWIEE882 pKa = 3.86QTQYY886 pKa = 11.37QSLSASNARR895 pKa = 11.84GANQSFFEE903 pKa = 4.51TMAHH907 pKa = 5.36TEE909 pKa = 4.08SLKK912 pKa = 9.59VTFSGYY918 pKa = 9.75VGNRR922 pKa = 11.84QLSVVDD928 pKa = 3.85SNYY931 pKa = 10.68GSGNWTASKK940 pKa = 10.32PRR942 pKa = 11.84VSEE945 pKa = 4.17LQWPDD950 pKa = 3.15PPSAVEE956 pKa = 3.76QLSVSKK962 pKa = 10.72NLRR965 pKa = 11.84SKK967 pKa = 10.2PAEE970 pKa = 3.89PTAGKK975 pKa = 10.05SVLADD980 pKa = 3.52SPPTPTAAPPEE991 pKa = 4.45KK992 pKa = 10.04KK993 pKa = 9.74PSPEE997 pKa = 4.26APTKK1001 pKa = 9.43PQAVDD1006 pKa = 3.36TPVKK1010 pKa = 10.29NKK1012 pKa = 10.45ASTPPPPKK1020 pKa = 9.86TATTNPAPPPAPDD1033 pKa = 3.42VAEE1036 pKa = 4.41SASDD1040 pKa = 3.22

Molecular weight:
112.52 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KFF5|A0A1L3KFF5_9VIRU Uncharacterized protein OS=Hubei toti-like virus 15 OX=1923303 PE=4 SV=1
MM1 pKa = 7.61GSPVCSYY8 pKa = 10.82KK9 pKa = 10.56YY10 pKa = 9.95RR11 pKa = 11.84LPLVRR16 pKa = 11.84QALPGPINSEE26 pKa = 4.24PYY28 pKa = 9.97HH29 pKa = 6.12IHH31 pKa = 6.8LPDD34 pKa = 3.38SFYY37 pKa = 11.47SALKK41 pKa = 9.61EE42 pKa = 4.08PKK44 pKa = 9.22EE45 pKa = 3.91LRR47 pKa = 11.84SRR49 pKa = 11.84PRR51 pKa = 11.84NVEE54 pKa = 3.45SLRR57 pKa = 11.84RR58 pKa = 11.84YY59 pKa = 8.89WGTTPKK65 pKa = 9.03WWPVPDD71 pKa = 3.99EE72 pKa = 4.61TDD74 pKa = 3.98CAWPLYY80 pKa = 10.46LYY82 pKa = 10.12YY83 pKa = 10.22QALKK87 pKa = 10.53EE88 pKa = 4.04CGQMNVAQEE97 pKa = 3.94IYY99 pKa = 10.02YY100 pKa = 10.22FLSKK104 pKa = 10.95VMSLWNRR111 pKa = 11.84RR112 pKa = 11.84CRR114 pKa = 11.84RR115 pKa = 11.84TGMSEE120 pKa = 3.44PSLYY124 pKa = 10.53VEE126 pKa = 4.98DD127 pKa = 5.09YY128 pKa = 9.74IRR130 pKa = 11.84HH131 pKa = 5.07MLKK134 pKa = 9.98QLSAYY139 pKa = 9.02SFPRR143 pKa = 11.84IFSYY147 pKa = 11.28GPAFMLLCHH156 pKa = 6.78LHH158 pKa = 6.55ALNAKK163 pKa = 9.71APYY166 pKa = 9.28EE167 pKa = 4.01RR168 pKa = 11.84EE169 pKa = 4.15TIISEE174 pKa = 3.67ITDD177 pKa = 3.39WVSASLNGRR186 pKa = 11.84KK187 pKa = 9.12KK188 pKa = 9.78EE189 pKa = 3.68IHH191 pKa = 6.01MPTFKK196 pKa = 10.43RR197 pKa = 11.84VLKK200 pKa = 8.88EE201 pKa = 4.23TVSKK205 pKa = 7.91WQKK208 pKa = 8.16YY209 pKa = 5.6THH211 pKa = 7.36DD212 pKa = 3.72SLSYY216 pKa = 11.12EE217 pKa = 4.23EE218 pKa = 4.72FANDD222 pKa = 3.4PFRR225 pKa = 11.84WGTSGGAPAVNMHH238 pKa = 5.18GTKK241 pKa = 9.53IRR243 pKa = 11.84SKK245 pKa = 8.43WAWAISQKK253 pKa = 9.94IDD255 pKa = 3.4EE256 pKa = 4.98KK257 pKa = 10.67KK258 pKa = 10.89GYY260 pKa = 8.93MDD262 pKa = 5.2GVDD265 pKa = 3.7LNKK268 pKa = 10.12RR269 pKa = 11.84ACSAQTMARR278 pKa = 11.84IALKK282 pKa = 10.6EE283 pKa = 3.9EE284 pKa = 4.15SQKK287 pKa = 9.35TRR289 pKa = 11.84EE290 pKa = 4.41IITTPIASYY299 pKa = 10.76LRR301 pKa = 11.84QTYY304 pKa = 10.72LMYY307 pKa = 11.17LLGKK311 pKa = 10.31APIPSPISSPAWLPSFEE328 pKa = 4.22EE329 pKa = 5.24GNYY332 pKa = 9.75SWYY335 pKa = 10.9GCIDD339 pKa = 3.52GEE341 pKa = 4.59RR342 pKa = 11.84FDD344 pKa = 3.7HH345 pKa = 5.83TVPKK349 pKa = 10.22EE350 pKa = 4.01AIICLLDD357 pKa = 3.49EE358 pKa = 5.63LGTISPEE365 pKa = 3.65ARR367 pKa = 11.84KK368 pKa = 9.8VADD371 pKa = 3.97EE372 pKa = 4.49EE373 pKa = 4.27IKK375 pKa = 10.6HH376 pKa = 5.36IQNLKK381 pKa = 9.74IEE383 pKa = 3.98WSGRR387 pKa = 11.84VWKK390 pKa = 11.15YY391 pKa = 10.9EE392 pKa = 3.66GGLLSGWRR400 pKa = 11.84FTSVLGSIISLSAAEE415 pKa = 4.03YY416 pKa = 10.84VVTKK420 pKa = 10.07TNTKK424 pKa = 9.98GAVQYY429 pKa = 11.27GVMGDD434 pKa = 3.66DD435 pKa = 3.42VVFYY439 pKa = 11.07SNVASIPPEE448 pKa = 4.42DD449 pKa = 3.27MVKK452 pKa = 10.49YY453 pKa = 10.15YY454 pKa = 11.34NEE456 pKa = 3.9FGLRR460 pKa = 11.84ANIHH464 pKa = 5.43KK465 pKa = 9.35TSSGEE470 pKa = 3.63QGEE473 pKa = 4.43FLRR476 pKa = 11.84KK477 pKa = 9.25VRR479 pKa = 11.84SKK481 pKa = 10.59GGSWAFPALDD491 pKa = 4.33LKK493 pKa = 10.19TITHH497 pKa = 6.94AAPWITSFQFVQEE510 pKa = 4.51EE511 pKa = 4.38EE512 pKa = 4.89CATAWHH518 pKa = 6.56TLTSRR523 pKa = 11.84LLPHH527 pKa = 6.55ATDD530 pKa = 4.28PNRR533 pKa = 11.84IANWSQKK540 pKa = 10.81HH541 pKa = 5.91NISDD545 pKa = 4.13LNRR548 pKa = 11.84SFGRR552 pKa = 11.84SYY554 pKa = 11.42DD555 pKa = 3.14WQAWLKK561 pKa = 9.82TPMTAGGGGYY571 pKa = 9.4VEE573 pKa = 4.6NSSPSVWTTISKK585 pKa = 10.22QKK587 pKa = 10.15SAPHH591 pKa = 5.44LTPYY595 pKa = 10.42EE596 pKa = 4.1KK597 pKa = 10.52LGQLVGTLPSKK608 pKa = 10.14RR609 pKa = 11.84TIKK612 pKa = 10.56KK613 pKa = 9.44IVLEE617 pKa = 3.98EE618 pKa = 3.92HH619 pKa = 5.64QLAKK623 pKa = 10.19IRR625 pKa = 11.84LLEE628 pKa = 3.94EE629 pKa = 3.81RR630 pKa = 11.84TNVTEE635 pKa = 3.95HH636 pKa = 7.19LEE638 pKa = 4.27FIPRR642 pKa = 11.84FKK644 pKa = 10.18RR645 pKa = 11.84TANITKK651 pKa = 9.12TMWRR655 pKa = 11.84ILNNDD660 pKa = 3.09MSVSEE665 pKa = 5.38LNTQLVTHH673 pKa = 7.09LPHH676 pKa = 7.3RR677 pKa = 11.84MRR679 pKa = 11.84MYY681 pKa = 10.49SGARR685 pKa = 11.84LASLLSTASKK695 pKa = 9.32TISSIPSIVHH705 pKa = 5.44TKK707 pKa = 9.7EE708 pKa = 3.63AMPVASRR715 pKa = 11.84TLTMLTKK722 pKa = 10.42QLMARR727 pKa = 11.84RR728 pKa = 11.84SGIPSTMAKK737 pKa = 10.1PLATLFAINHH747 pKa = 5.66YY748 pKa = 9.24KK749 pKa = 9.66DD750 pKa = 3.41TVISYY755 pKa = 7.47GTWW758 pKa = 2.87

Molecular weight:
86.52 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1798

758

1040

899.0

99.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.398 ± 0.899

1.001 ± 0.119

3.782 ± 0.478

4.95 ± 0.883

2.392 ± 0.242

4.95 ± 0.293

2.447 ± 0.291

5.951 ± 0.075

5.117 ± 1.03

7.786 ± 0.673

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.336 ± 0.362

4.449 ± 0.651

7.341 ± 0.982

3.393 ± 0.313

4.561 ± 0.879

10.79 ± 1.078

8.621 ± 0.956

5.339 ± 0.377

2.392 ± 0.495

4.004 ± 0.392

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski