Hubei toti-like virus 15
Average proteome isoelectric point is 7.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KFF5|A0A1L3KFF5_9VIRU Uncharacterized protein OS=Hubei toti-like virus 15 OX=1923303 PE=4 SV=1
MM1 pKa = 7.9 ADD3 pKa = 3.08 ATTPSVSPLTEE14 pKa = 4.02 PQAEE18 pKa = 4.2 NPTTSSRR25 pKa = 11.84 EE26 pKa = 3.76 DD27 pKa = 3.1 GFASVRR33 pKa = 11.84 NLQSWIKK40 pKa = 8.97 TPLSIVGSNNAPLSAYY56 pKa = 7.28 PTGFDD61 pKa = 3.21 TASIDD66 pKa = 3.82 GQKK69 pKa = 9.5 KK70 pKa = 6.9 QQYY73 pKa = 8.23 STFSVLGASYY83 pKa = 11.02 LSYY86 pKa = 11.17 LCLRR90 pKa = 11.84 YY91 pKa = 9.92 SVISSLQQNTQTQTQGEE108 pKa = 4.4 YY109 pKa = 10.66 DD110 pKa = 3.69 IVNPPPGAVAALTIAQQAAYY130 pKa = 9.67 HH131 pKa = 5.82 NKK133 pKa = 8.85 EE134 pKa = 3.81 LLYY137 pKa = 10.11 TRR139 pKa = 11.84 AHH141 pKa = 6.26 AFVPTAAIARR151 pKa = 11.84 KK152 pKa = 6.65 MARR155 pKa = 11.84 IIKK158 pKa = 8.27 MGPATLSIPTVQSLQTTTPIDD179 pKa = 3.65 LSEE182 pKa = 4.14 QNIPTEE188 pKa = 4.28 TNNAVAASIGTTSSSTTLRR207 pKa = 11.84 SSSSTTSANSGPSDD221 pKa = 3.43 HH222 pKa = 6.68 ATGRR226 pKa = 11.84 SINPQPDD233 pKa = 3.67 DD234 pKa = 4.37 PPCEE238 pKa = 4.62 IISKK242 pKa = 10.25 PEE244 pKa = 3.76 VIPYY248 pKa = 9.84 RR249 pKa = 11.84 VVKK252 pKa = 9.76 TSHH255 pKa = 6.71 LAGSSEE261 pKa = 4.37 VTSIPYY267 pKa = 9.85 SEE269 pKa = 4.85 IYY271 pKa = 10.21 NAISASLSSEE281 pKa = 3.69 ANTAIRR287 pKa = 11.84 ATLKK291 pKa = 10.75 SALVCEE297 pKa = 4.34 ASTWSVLVKK306 pKa = 9.93 TLSQEE311 pKa = 4.09 SGSVCDD317 pKa = 3.43 VAPLVRR323 pKa = 11.84 LATLSYY329 pKa = 10.82 ALSSTAASFDD339 pKa = 3.39 TMPYY343 pKa = 10.53 KK344 pKa = 10.79 LFRR347 pKa = 11.84 NMSVSYY353 pKa = 10.42 QGTAIHH359 pKa = 6.91 APDD362 pKa = 4.77 PNLPHH367 pKa = 7.36 SIIAMPLEE375 pKa = 4.23 VFTTFMVGTAMTSPLPSIFLPSEE398 pKa = 3.85 VDD400 pKa = 3.45 KK401 pKa = 11.19 GWVGIPISEE410 pKa = 3.9 QDD412 pKa = 3.33 ARR414 pKa = 11.84 LPCIRR419 pKa = 11.84 EE420 pKa = 4.13 IITSHH425 pKa = 6.65 ISSDD429 pKa = 3.57 LTFGTITKK437 pKa = 9.25 RR438 pKa = 11.84 YY439 pKa = 8.27 NVSAPGDD446 pKa = 3.32 ITGQLLCNPASNMSYY461 pKa = 9.01 VHH463 pKa = 6.65 GPRR466 pKa = 11.84 HH467 pKa = 6.03 VILIIKK473 pKa = 10.06 DD474 pKa = 3.24 RR475 pKa = 11.84 GSTNEE480 pKa = 3.94 TDD482 pKa = 3.81 SIQIGGLQVPVYY494 pKa = 10.52 RR495 pKa = 11.84 NRR497 pKa = 11.84 TGQAPMITPANWTTEE512 pKa = 3.33 WVRR515 pKa = 11.84 YY516 pKa = 9.84 YY517 pKa = 10.12 NTANSAKK524 pKa = 9.96 ILSNIQNAWNHH535 pKa = 5.41 LTSKK539 pKa = 9.95 TITLDD544 pKa = 3.26 TAGRR548 pKa = 11.84 ALSVAAEE555 pKa = 4.31 LTLATPPGLYY565 pKa = 10.13 LHH567 pKa = 7.02 PNSNGIAYY575 pKa = 9.9 DD576 pKa = 3.43 YY577 pKa = 11.08 DD578 pKa = 4.4 RR579 pKa = 11.84 PLSGAFVIHH588 pKa = 6.23 VRR590 pKa = 11.84 DD591 pKa = 3.7 NNGTCKK597 pKa = 10.17 LDD599 pKa = 3.42 KK600 pKa = 9.58 TDD602 pKa = 3.66 YY603 pKa = 8.77 MADD606 pKa = 3.69 VITLTTPGSDD616 pKa = 3.2 QTVKK620 pKa = 10.98 KK621 pKa = 10.7 VMAGINIASISPLHH635 pKa = 6.35 LPPSGLVPTTYY646 pKa = 9.94 TSSGPSVYY654 pKa = 10.53 AANDD658 pKa = 3.59 PDD660 pKa = 3.85 LTKK663 pKa = 11.06 ASDD666 pKa = 3.86 TYY668 pKa = 10.85 HH669 pKa = 6.62 IPCSSTLKK677 pKa = 10.51 RR678 pKa = 11.84 LSVYY682 pKa = 10.51 LGLINRR688 pKa = 11.84 TSTPYY693 pKa = 10.62 YY694 pKa = 9.62 FASAEE699 pKa = 4.02 ALSGWVHH706 pKa = 6.15 MLAHH710 pKa = 6.75 ANSANTAAMLQEE722 pKa = 3.95 IDD724 pKa = 3.5 MPARR728 pKa = 11.84 DD729 pKa = 3.37 WTGFNTDD736 pKa = 2.24 WSEE739 pKa = 3.86 NNRR742 pKa = 11.84 FEE744 pKa = 5.29 EE745 pKa = 4.18 INSLKK750 pKa = 9.8 RR751 pKa = 11.84 TVSMDD756 pKa = 3.45 MIKK759 pKa = 10.41 HH760 pKa = 5.81 FEE762 pKa = 4.13 AEE764 pKa = 4.36 SEE766 pKa = 4.27 FEE768 pKa = 4.23 SWDD771 pKa = 3.53 SYY773 pKa = 11.83 DD774 pKa = 4.27 PDD776 pKa = 4.58 WISTAYY782 pKa = 10.46 GGIDD786 pKa = 3.34 PFSNTMWWQQSPVPSHH802 pKa = 7.44 FLLQWYY808 pKa = 8.78 KK809 pKa = 10.58 KK810 pKa = 9.68 LKK812 pKa = 9.43 TVSCPEE818 pKa = 3.9 TRR820 pKa = 11.84 PGQNPLLYY828 pKa = 10.98 VNGRR832 pKa = 11.84 KK833 pKa = 9.38 KK834 pKa = 10.45 FAVTMTISNKK844 pKa = 9.77 PSWEE848 pKa = 4.03 WIIASIDD855 pKa = 3.2 THH857 pKa = 6.16 HH858 pKa = 6.95 RR859 pKa = 11.84 HH860 pKa = 6.51 PIVVDD865 pKa = 3.05 TSYY868 pKa = 11.1 RR869 pKa = 11.84 QNRR872 pKa = 11.84 ANAVLGSWIEE882 pKa = 3.86 QTQYY886 pKa = 11.37 QSLSASNARR895 pKa = 11.84 GANQSFFEE903 pKa = 4.51 TMAHH907 pKa = 5.36 TEE909 pKa = 4.08 SLKK912 pKa = 9.59 VTFSGYY918 pKa = 9.75 VGNRR922 pKa = 11.84 QLSVVDD928 pKa = 3.85 SNYY931 pKa = 10.68 GSGNWTASKK940 pKa = 10.32 PRR942 pKa = 11.84 VSEE945 pKa = 4.17 LQWPDD950 pKa = 3.15 PPSAVEE956 pKa = 3.76 QLSVSKK962 pKa = 10.72 NLRR965 pKa = 11.84 SKK967 pKa = 10.2 PAEE970 pKa = 3.89 PTAGKK975 pKa = 10.05 SVLADD980 pKa = 3.52 SPPTPTAAPPEE991 pKa = 4.45 KK992 pKa = 10.04 KK993 pKa = 9.74 PSPEE997 pKa = 4.26 APTKK1001 pKa = 9.43 PQAVDD1006 pKa = 3.36 TPVKK1010 pKa = 10.29 NKK1012 pKa = 10.45 ASTPPPPKK1020 pKa = 9.86 TATTNPAPPPAPDD1033 pKa = 3.42 VAEE1036 pKa = 4.41 SASDD1040 pKa = 3.22
Molecular weight: 112.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.917
IPC2_protein 5.893
IPC_protein 5.982
Toseland 6.415
ProMoST 6.3
Dawson 6.287
Bjellqvist 6.287
Wikipedia 6.313
Rodwell 6.275
Grimsley 6.576
Solomon 6.287
Lehninger 6.287
Nozaki 6.561
DTASelect 6.751
Thurlkill 6.781
EMBOSS 6.737
Sillero 6.664
Patrickios 3.986
IPC_peptide 6.3
IPC2_peptide 6.605
IPC2.peptide.svr19 6.627
Protein with the highest isoelectric point:
>tr|A0A1L3KFF5|A0A1L3KFF5_9VIRU Uncharacterized protein OS=Hubei toti-like virus 15 OX=1923303 PE=4 SV=1
MM1 pKa = 7.61 GSPVCSYY8 pKa = 10.82 KK9 pKa = 10.56 YY10 pKa = 9.95 RR11 pKa = 11.84 LPLVRR16 pKa = 11.84 QALPGPINSEE26 pKa = 4.24 PYY28 pKa = 9.97 HH29 pKa = 6.12 IHH31 pKa = 6.8 LPDD34 pKa = 3.38 SFYY37 pKa = 11.47 SALKK41 pKa = 9.61 EE42 pKa = 4.08 PKK44 pKa = 9.22 EE45 pKa = 3.91 LRR47 pKa = 11.84 SRR49 pKa = 11.84 PRR51 pKa = 11.84 NVEE54 pKa = 3.45 SLRR57 pKa = 11.84 RR58 pKa = 11.84 YY59 pKa = 8.89 WGTTPKK65 pKa = 9.03 WWPVPDD71 pKa = 3.99 EE72 pKa = 4.61 TDD74 pKa = 3.98 CAWPLYY80 pKa = 10.46 LYY82 pKa = 10.12 YY83 pKa = 10.22 QALKK87 pKa = 10.53 EE88 pKa = 4.04 CGQMNVAQEE97 pKa = 3.94 IYY99 pKa = 10.02 YY100 pKa = 10.22 FLSKK104 pKa = 10.95 VMSLWNRR111 pKa = 11.84 RR112 pKa = 11.84 CRR114 pKa = 11.84 RR115 pKa = 11.84 TGMSEE120 pKa = 3.44 PSLYY124 pKa = 10.53 VEE126 pKa = 4.98 DD127 pKa = 5.09 YY128 pKa = 9.74 IRR130 pKa = 11.84 HH131 pKa = 5.07 MLKK134 pKa = 9.98 QLSAYY139 pKa = 9.02 SFPRR143 pKa = 11.84 IFSYY147 pKa = 11.28 GPAFMLLCHH156 pKa = 6.78 LHH158 pKa = 6.55 ALNAKK163 pKa = 9.71 APYY166 pKa = 9.28 EE167 pKa = 4.01 RR168 pKa = 11.84 EE169 pKa = 4.15 TIISEE174 pKa = 3.67 ITDD177 pKa = 3.39 WVSASLNGRR186 pKa = 11.84 KK187 pKa = 9.12 KK188 pKa = 9.78 EE189 pKa = 3.68 IHH191 pKa = 6.01 MPTFKK196 pKa = 10.43 RR197 pKa = 11.84 VLKK200 pKa = 8.88 EE201 pKa = 4.23 TVSKK205 pKa = 7.91 WQKK208 pKa = 8.16 YY209 pKa = 5.6 THH211 pKa = 7.36 DD212 pKa = 3.72 SLSYY216 pKa = 11.12 EE217 pKa = 4.23 EE218 pKa = 4.72 FANDD222 pKa = 3.4 PFRR225 pKa = 11.84 WGTSGGAPAVNMHH238 pKa = 5.18 GTKK241 pKa = 9.53 IRR243 pKa = 11.84 SKK245 pKa = 8.43 WAWAISQKK253 pKa = 9.94 IDD255 pKa = 3.4 EE256 pKa = 4.98 KK257 pKa = 10.67 KK258 pKa = 10.89 GYY260 pKa = 8.93 MDD262 pKa = 5.2 GVDD265 pKa = 3.7 LNKK268 pKa = 10.12 RR269 pKa = 11.84 ACSAQTMARR278 pKa = 11.84 IALKK282 pKa = 10.6 EE283 pKa = 3.9 EE284 pKa = 4.15 SQKK287 pKa = 9.35 TRR289 pKa = 11.84 EE290 pKa = 4.41 IITTPIASYY299 pKa = 10.76 LRR301 pKa = 11.84 QTYY304 pKa = 10.72 LMYY307 pKa = 11.17 LLGKK311 pKa = 10.31 APIPSPISSPAWLPSFEE328 pKa = 4.22 EE329 pKa = 5.24 GNYY332 pKa = 9.75 SWYY335 pKa = 10.9 GCIDD339 pKa = 3.52 GEE341 pKa = 4.59 RR342 pKa = 11.84 FDD344 pKa = 3.7 HH345 pKa = 5.83 TVPKK349 pKa = 10.22 EE350 pKa = 4.01 AIICLLDD357 pKa = 3.49 EE358 pKa = 5.63 LGTISPEE365 pKa = 3.65 ARR367 pKa = 11.84 KK368 pKa = 9.8 VADD371 pKa = 3.97 EE372 pKa = 4.49 EE373 pKa = 4.27 IKK375 pKa = 10.6 HH376 pKa = 5.36 IQNLKK381 pKa = 9.74 IEE383 pKa = 3.98 WSGRR387 pKa = 11.84 VWKK390 pKa = 11.15 YY391 pKa = 10.9 EE392 pKa = 3.66 GGLLSGWRR400 pKa = 11.84 FTSVLGSIISLSAAEE415 pKa = 4.03 YY416 pKa = 10.84 VVTKK420 pKa = 10.07 TNTKK424 pKa = 9.98 GAVQYY429 pKa = 11.27 GVMGDD434 pKa = 3.66 DD435 pKa = 3.42 VVFYY439 pKa = 11.07 SNVASIPPEE448 pKa = 4.42 DD449 pKa = 3.27 MVKK452 pKa = 10.49 YY453 pKa = 10.15 YY454 pKa = 11.34 NEE456 pKa = 3.9 FGLRR460 pKa = 11.84 ANIHH464 pKa = 5.43 KK465 pKa = 9.35 TSSGEE470 pKa = 3.63 QGEE473 pKa = 4.43 FLRR476 pKa = 11.84 KK477 pKa = 9.25 VRR479 pKa = 11.84 SKK481 pKa = 10.59 GGSWAFPALDD491 pKa = 4.33 LKK493 pKa = 10.19 TITHH497 pKa = 6.94 AAPWITSFQFVQEE510 pKa = 4.51 EE511 pKa = 4.38 EE512 pKa = 4.89 CATAWHH518 pKa = 6.56 TLTSRR523 pKa = 11.84 LLPHH527 pKa = 6.55 ATDD530 pKa = 4.28 PNRR533 pKa = 11.84 IANWSQKK540 pKa = 10.81 HH541 pKa = 5.91 NISDD545 pKa = 4.13 LNRR548 pKa = 11.84 SFGRR552 pKa = 11.84 SYY554 pKa = 11.42 DD555 pKa = 3.14 WQAWLKK561 pKa = 9.82 TPMTAGGGGYY571 pKa = 9.4 VEE573 pKa = 4.6 NSSPSVWTTISKK585 pKa = 10.22 QKK587 pKa = 10.15 SAPHH591 pKa = 5.44 LTPYY595 pKa = 10.42 EE596 pKa = 4.1 KK597 pKa = 10.52 LGQLVGTLPSKK608 pKa = 10.14 RR609 pKa = 11.84 TIKK612 pKa = 10.56 KK613 pKa = 9.44 IVLEE617 pKa = 3.98 EE618 pKa = 3.92 HH619 pKa = 5.64 QLAKK623 pKa = 10.19 IRR625 pKa = 11.84 LLEE628 pKa = 3.94 EE629 pKa = 3.81 RR630 pKa = 11.84 TNVTEE635 pKa = 3.95 HH636 pKa = 7.19 LEE638 pKa = 4.27 FIPRR642 pKa = 11.84 FKK644 pKa = 10.18 RR645 pKa = 11.84 TANITKK651 pKa = 9.12 TMWRR655 pKa = 11.84 ILNNDD660 pKa = 3.09 MSVSEE665 pKa = 5.38 LNTQLVTHH673 pKa = 7.09 LPHH676 pKa = 7.3 RR677 pKa = 11.84 MRR679 pKa = 11.84 MYY681 pKa = 10.49 SGARR685 pKa = 11.84 LASLLSTASKK695 pKa = 9.32 TISSIPSIVHH705 pKa = 5.44 TKK707 pKa = 9.7 EE708 pKa = 3.63 AMPVASRR715 pKa = 11.84 TLTMLTKK722 pKa = 10.42 QLMARR727 pKa = 11.84 RR728 pKa = 11.84 SGIPSTMAKK737 pKa = 10.1 PLATLFAINHH747 pKa = 5.66 YY748 pKa = 9.24 KK749 pKa = 9.66 DD750 pKa = 3.41 TVISYY755 pKa = 7.47 GTWW758 pKa = 2.87
Molecular weight: 86.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.792
IPC2_protein 8.829
IPC_protein 8.712
Toseland 9.311
ProMoST 9.18
Dawson 9.648
Bjellqvist 9.399
Wikipedia 9.838
Rodwell 9.853
Grimsley 9.736
Solomon 9.663
Lehninger 9.604
Nozaki 9.443
DTASelect 9.355
Thurlkill 9.472
EMBOSS 9.765
Sillero 9.589
Patrickios 4.647
IPC_peptide 9.648
IPC2_peptide 8.053
IPC2.peptide.svr19 7.778
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1798
758
1040
899.0
99.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.398 ± 0.899
1.001 ± 0.119
3.782 ± 0.478
4.95 ± 0.883
2.392 ± 0.242
4.95 ± 0.293
2.447 ± 0.291
5.951 ± 0.075
5.117 ± 1.03
7.786 ± 0.673
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.336 ± 0.362
4.449 ± 0.651
7.341 ± 0.982
3.393 ± 0.313
4.561 ± 0.879
10.79 ± 1.078
8.621 ± 0.956
5.339 ± 0.377
2.392 ± 0.495
4.004 ± 0.392
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here