Prevotella sp. AM42-24

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; unclassified Prevotella

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3181 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A374BZ11|A0A374BZ11_9BACT Uncharacterized protein (Fragment) OS=Prevotella sp. AM42-24 OX=2293125 GN=DW917_12240 PE=4 SV=1
MM1 pKa = 8.19IFMALAALLCLASCSDD17 pKa = 4.49DD18 pKa = 3.95DD19 pKa = 5.72SGIQLTEE26 pKa = 4.23DD27 pKa = 3.89EE28 pKa = 4.61VTGDD32 pKa = 4.05IITGKK37 pKa = 10.4QIVINTLTTYY47 pKa = 10.74TDD49 pKa = 3.87GGCKK53 pKa = 10.39VNVNGANSLL62 pKa = 3.79

Molecular weight: 6.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A374CEA2|A0A374CEA2_9BACT DUF4976 domain-containing protein OS=Prevotella sp. AM42-24 OX=2293125 GN=DW917_05245 PE=3 SV=1
MM1 pKa = 7.45FRR3 pKa = 11.84RR4 pKa = 11.84KK5 pKa = 9.38VQSIQTLLPDD15 pKa = 3.76FLRR18 pKa = 11.84RR19 pKa = 11.84QGLEE23 pKa = 3.53SPLRR27 pKa = 11.84QRR29 pKa = 11.84RR30 pKa = 11.84LLTSWDD36 pKa = 3.76EE37 pKa = 4.28VVGEE41 pKa = 4.3PIARR45 pKa = 11.84YY46 pKa = 7.19TSEE49 pKa = 5.07RR50 pKa = 11.84FIKK53 pKa = 10.63NQTLYY58 pKa = 11.33VKK60 pKa = 9.67VHH62 pKa = 6.03NPALRR67 pKa = 11.84ADD69 pKa = 3.87LSMGRR74 pKa = 11.84SLLVKK79 pKa = 10.23RR80 pKa = 11.84LNEE83 pKa = 3.7RR84 pKa = 11.84AGAQVIVDD92 pKa = 3.36IKK94 pKa = 10.73FYY96 pKa = 11.28

Molecular weight: 11.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3181

0

3181

1115435

31

3234

350.7

39.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.056 ± 0.041

1.316 ± 0.017

5.855 ± 0.033

6.392 ± 0.041

4.489 ± 0.03

6.73 ± 0.046

2.032 ± 0.021

6.8 ± 0.044

7.364 ± 0.036

8.599 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.955 ± 0.021

5.361 ± 0.036

3.429 ± 0.019

3.641 ± 0.027

4.235 ± 0.034

6.161 ± 0.034

5.512 ± 0.038

6.324 ± 0.033

1.259 ± 0.017

4.49 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski