Streptococcus satellite phage Javan260
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZJI8|A0A4D5ZJI8_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan260 OX=2558596 GN=JavanS260_0011 PE=4 SV=1
MM1 pKa = 7.39 LVTYY5 pKa = 8.27 PALFYY10 pKa = 11.35 YY11 pKa = 10.68 DD12 pKa = 4.04 DD13 pKa = 4.01 TDD15 pKa = 3.86 GANAPYY21 pKa = 10.03 FVTFPDD27 pKa = 4.61 FEE29 pKa = 5.3 HH30 pKa = 7.07 SATQGEE36 pKa = 4.54 DD37 pKa = 3.02 MANAMAMASDD47 pKa = 3.31 WLGIHH52 pKa = 6.56 LADD55 pKa = 4.36 YY56 pKa = 10.61 IEE58 pKa = 4.46 NGRR61 pKa = 11.84 DD62 pKa = 3.13 IPTPAPINTLSLADD76 pKa = 3.94 NNPFRR81 pKa = 11.84 DD82 pKa = 3.99 DD83 pKa = 3.55 EE84 pKa = 5.07 DD85 pKa = 4.4 IEE87 pKa = 5.57 LIYY90 pKa = 10.9 DD91 pKa = 3.72 PSKK94 pKa = 11.33 SFISMVMVDD103 pKa = 3.25 VAEE106 pKa = 4.14 YY107 pKa = 10.49 LGSQEE112 pKa = 4.15 PVKK115 pKa = 10.18 KK116 pKa = 9.12 TLTIPRR122 pKa = 11.84 WADD125 pKa = 3.11 TLGRR129 pKa = 11.84 DD130 pKa = 3.39 LGLNFSQTLTDD141 pKa = 5.45 AIADD145 pKa = 4.03 KK146 pKa = 10.56 KK147 pKa = 10.31 IHH149 pKa = 5.92 AA150 pKa = 5.0
Molecular weight: 16.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.101
IPC2_protein 3.986
IPC_protein 3.986
Toseland 3.757
ProMoST 4.151
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.935
Rodwell 3.808
Grimsley 3.668
Solomon 3.973
Lehninger 3.935
Nozaki 4.101
DTASelect 4.368
Thurlkill 3.821
EMBOSS 3.948
Sillero 4.101
Patrickios 3.312
IPC_peptide 3.973
IPC2_peptide 4.075
IPC2.peptide.svr19 4.022
Protein with the highest isoelectric point:
>tr|A0A4D5ZM32|A0A4D5ZM32_9VIRU DNA primase OS=Streptococcus satellite phage Javan260 OX=2558596 GN=JavanS260_0006 PE=4 SV=1
MM1 pKa = 7.63 KK2 pKa = 10.09 IKK4 pKa = 10.66 PITKK8 pKa = 9.17 KK9 pKa = 10.51 NGAIVYY15 pKa = 8.3 RR16 pKa = 11.84 ANVYY20 pKa = 10.65 LGTDD24 pKa = 2.95 QVTGKK29 pKa = 9.97 KK30 pKa = 10.33 AKK32 pKa = 9.51 TSVTGRR38 pKa = 11.84 TRR40 pKa = 11.84 KK41 pKa = 8.76 EE42 pKa = 3.84 VKK44 pKa = 10.02 QKK46 pKa = 10.96 AKK48 pKa = 10.04 HH49 pKa = 5.68 AQDD52 pKa = 3.62 EE53 pKa = 5.21 FISNGYY59 pKa = 7.35 TVTKK63 pKa = 9.88 VVPIKK68 pKa = 10.67 NYY70 pKa = 10.29 QEE72 pKa = 4.68 LAEE75 pKa = 4.74 LWLEE79 pKa = 4.32 SYY81 pKa = 10.85 QLTVKK86 pKa = 9.85 PQTFIATKK94 pKa = 10.24 RR95 pKa = 11.84 MLYY98 pKa = 10.01 NHH100 pKa = 7.89 LIPVFGALKK109 pKa = 10.32 LNKK112 pKa = 10.01 LSVSYY117 pKa = 10.64 IQGFINDD124 pKa = 4.13 LSAEE128 pKa = 4.15 LVHH131 pKa = 6.38 YY132 pKa = 10.48 SVVHH136 pKa = 5.68 SINRR140 pKa = 11.84 RR141 pKa = 11.84 VLQYY145 pKa = 10.88 GVSLQLLPFNPARR158 pKa = 11.84 DD159 pKa = 3.54 VMLPKK164 pKa = 10.25 VPKK167 pKa = 10.2 KK168 pKa = 9.64 EE169 pKa = 3.73 NKK171 pKa = 9.48 AIKK174 pKa = 10.16 FIAPEE179 pKa = 3.97 DD180 pKa = 3.88 LKK182 pKa = 11.69 ALMAYY187 pKa = 8.24 MEE189 pKa = 4.47 KK190 pKa = 10.52 LANKK194 pKa = 9.07 KK195 pKa = 9.8 FSYY198 pKa = 10.51 FFDD201 pKa = 3.67 YY202 pKa = 11.08 VLYY205 pKa = 10.68 SVLLATGCRR214 pKa = 11.84 FGEE217 pKa = 4.58 VVALEE222 pKa = 4.12 WSDD225 pKa = 3.32 IDD227 pKa = 5.09 LEE229 pKa = 4.52 NGTISITKK237 pKa = 9.93 NYY239 pKa = 10.09 SRR241 pKa = 11.84 LLKK244 pKa = 10.89 LIGTPKK250 pKa = 9.91 SKK252 pKa = 10.87 AGVRR256 pKa = 11.84 VISIDD261 pKa = 3.23 KK262 pKa = 9.23 KK263 pKa = 9.18 TINLLRR269 pKa = 11.84 LYY271 pKa = 10.58 KK272 pKa = 10.31 NRR274 pKa = 11.84 QRR276 pKa = 11.84 QIFIEE281 pKa = 4.08 TGARR285 pKa = 11.84 VSAVVFATPTKK296 pKa = 9.74 EE297 pKa = 4.11 YY298 pKa = 10.79 QNMATRR304 pKa = 11.84 QEE306 pKa = 4.21 SLDD309 pKa = 3.62 RR310 pKa = 11.84 RR311 pKa = 11.84 ITEE314 pKa = 3.41 IGIPRR319 pKa = 11.84 FTFHH323 pKa = 7.74 AFRR326 pKa = 11.84 HH327 pKa = 4.69 THH329 pKa = 6.92 ASLLLNAGISYY340 pKa = 10.68 KK341 pKa = 10.23 EE342 pKa = 3.61 LQYY345 pKa = 11.49 RR346 pKa = 11.84 LGHH349 pKa = 6.19 ATLAMTMDD357 pKa = 4.28 IYY359 pKa = 11.69 SHH361 pKa = 7.7 LSMDD365 pKa = 3.69 KK366 pKa = 10.11 EE367 pKa = 4.32 KK368 pKa = 10.95 EE369 pKa = 3.93 AVSYY373 pKa = 9.5 YY374 pKa = 10.62 EE375 pKa = 4.12 KK376 pKa = 10.7 AINSLL381 pKa = 3.57
Molecular weight: 43.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.103
IPC2_protein 9.355
IPC_protein 9.282
Toseland 9.97
ProMoST 9.648
Dawson 10.175
Bjellqvist 9.838
Wikipedia 10.35
Rodwell 10.657
Grimsley 10.248
Solomon 10.189
Lehninger 10.16
Nozaki 9.94
DTASelect 9.838
Thurlkill 10.014
EMBOSS 10.365
Sillero 10.087
Patrickios 10.058
IPC_peptide 10.189
IPC2_peptide 8.331
IPC2.peptide.svr19 8.389
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
21
0
21
3076
37
507
146.5
16.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.722 ± 0.649
0.65 ± 0.202
6.404 ± 0.547
7.38 ± 0.497
3.771 ± 0.289
4.714 ± 0.376
1.658 ± 0.231
7.412 ± 0.373
8.713 ± 0.568
10.468 ± 0.49
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.568 ± 0.286
5.592 ± 0.346
3.771 ± 0.475
4.551 ± 0.398
4.876 ± 0.394
5.234 ± 0.372
6.014 ± 0.304
5.169 ± 0.481
0.943 ± 0.15
4.389 ± 0.36
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here