Pelotomaculum schinkii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Pelotomaculum

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4132 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y7RCE5|A0A4Y7RCE5_9FIRM Stage III sporulation protein AE OS=Pelotomaculum schinkii OX=78350 GN=spoIIIAE PE=4 SV=1
MM1 pKa = 7.67RR2 pKa = 11.84SVLRR6 pKa = 11.84KK7 pKa = 8.94ILSMLLVLCLWTALMPFSVLAAEE30 pKa = 4.78GDD32 pKa = 3.44ICEE35 pKa = 4.56VNGTTYY41 pKa = 10.29ATLDD45 pKa = 3.64DD46 pKa = 5.08ALDD49 pKa = 4.05AVHH52 pKa = 6.54PTGGGTIRR60 pKa = 11.84LLEE63 pKa = 4.54NINYY67 pKa = 10.2DD68 pKa = 3.18GGIVIDD74 pKa = 4.04RR75 pKa = 11.84EE76 pKa = 4.65GITFDD81 pKa = 5.14LDD83 pKa = 3.72GFTLNVDD90 pKa = 3.24NSTGPGLKK98 pKa = 10.28VIGGGTVEE106 pKa = 5.83FIDD109 pKa = 4.16EE110 pKa = 4.29GTTGSFNVTGTEE122 pKa = 3.9YY123 pKa = 11.04GVWADD128 pKa = 3.56YY129 pKa = 11.38GSVVWVNNVTASGEE143 pKa = 4.27SSCGVKK149 pKa = 9.26ATEE152 pKa = 3.89YY153 pKa = 11.09ASIIVEE159 pKa = 4.18GNVIATNTNSIGVGVEE175 pKa = 3.73SGGEE179 pKa = 4.1VNVKK183 pKa = 10.66GDD185 pKa = 3.32VTANGIEE192 pKa = 4.53GIGIKK197 pKa = 10.22AYY199 pKa = 10.56DD200 pKa = 3.9VVCDD204 pKa = 3.52EE205 pKa = 5.2AEE207 pKa = 4.29VIVEE211 pKa = 4.14GDD213 pKa = 3.5VTASGGDD220 pKa = 3.62SVGVLAIDD228 pKa = 3.68SYY230 pKa = 10.36VTVGGNVTGDD240 pKa = 3.16KK241 pKa = 10.96GGVDD245 pKa = 3.89AEE247 pKa = 4.49DD248 pKa = 3.43SGIEE252 pKa = 3.76ITGDD256 pKa = 3.43VQSSDD261 pKa = 2.91EE262 pKa = 4.11SGYY265 pKa = 10.77GIRR268 pKa = 11.84ATDD271 pKa = 3.35NCDD274 pKa = 2.6IDD276 pKa = 3.98INGNVRR282 pKa = 11.84SNGTGVWIWAEE293 pKa = 4.05GTADD297 pKa = 3.7FSEE300 pKa = 4.09ITIDD304 pKa = 3.88GVIDD308 pKa = 3.69APEE311 pKa = 4.15YY312 pKa = 10.41IRR314 pKa = 11.84IGIDD318 pKa = 2.89TFDD321 pKa = 4.62FEE323 pKa = 6.57DD324 pKa = 5.53GYY326 pKa = 11.27LDD328 pKa = 4.22PDD330 pKa = 3.95MIDD333 pKa = 3.56GYY335 pKa = 10.59RR336 pKa = 11.84IYY338 pKa = 11.18VDD340 pKa = 3.56EE341 pKa = 4.47SLGAVLVAEE350 pKa = 4.81FAGGSGSSEE359 pKa = 3.66EE360 pKa = 4.42DD361 pKa = 3.62PYY363 pKa = 11.69LVAHH367 pKa = 7.34AAQLYY372 pKa = 8.69NVRR375 pKa = 11.84NHH377 pKa = 6.57LDD379 pKa = 3.06KK380 pKa = 10.97HH381 pKa = 6.02FKK383 pKa = 10.49LIEE386 pKa = 5.06DD387 pKa = 4.15IDD389 pKa = 4.23LSGYY393 pKa = 8.05STGDD397 pKa = 2.63GWEE400 pKa = 4.56PIGDD404 pKa = 4.15NIDD407 pKa = 3.82PFTGTFDD414 pKa = 3.6GGEE417 pKa = 3.93EE418 pKa = 5.33GYY420 pKa = 9.12TISGLVIDD428 pKa = 6.15RR429 pKa = 11.84ITTAYY434 pKa = 10.31VGLFGCTGAEE444 pKa = 3.83AEE446 pKa = 4.48IRR448 pKa = 11.84DD449 pKa = 4.44LRR451 pKa = 11.84LADD454 pKa = 3.7VNVTGSVYY462 pKa = 10.82VGGLVGRR469 pKa = 11.84NYY471 pKa = 10.89GQVTNSYY478 pKa = 8.96ATGDD482 pKa = 3.33VTARR486 pKa = 11.84SDD488 pKa = 3.29GGGLVGQNEE497 pKa = 4.3GSITDD502 pKa = 3.79SYY504 pKa = 11.81AIGTVTDD511 pKa = 3.31VWSGTFGGLVGHH523 pKa = 6.68NNGTITNCHH532 pKa = 6.3AEE534 pKa = 4.27VTVKK538 pKa = 10.79SNEE541 pKa = 4.02SMVGGLVGEE550 pKa = 4.16NDD552 pKa = 3.65GGISEE557 pKa = 4.77SYY559 pKa = 10.94AVGEE563 pKa = 4.28VEE565 pKa = 4.61GKK567 pKa = 10.19SEE569 pKa = 3.98VGGLVGYY576 pKa = 10.69NSGGNIDD583 pKa = 3.76RR584 pKa = 11.84SYY586 pKa = 11.03ATGAVTGTEE595 pKa = 3.59TGIYY599 pKa = 7.24YY600 pKa = 9.34TYY602 pKa = 11.09VGGLVGINAGPISEE616 pKa = 4.4SYY618 pKa = 11.17ARR620 pKa = 11.84GAVSGQEE627 pKa = 4.19DD628 pKa = 3.25IGGLVGYY635 pKa = 10.03NSGSGSVTNSYY646 pKa = 9.1ATGTVNGYY654 pKa = 10.4SYY656 pKa = 10.93IGGLAGLNLGNIMDD670 pKa = 4.79SYY672 pKa = 11.01WDD674 pKa = 3.87TEE676 pKa = 4.15TSLQRR681 pKa = 11.84SSDD684 pKa = 3.32GGTGKK689 pKa = 8.75ATAEE693 pKa = 4.26MKK695 pKa = 10.13QQATFTDD702 pKa = 3.34WDD704 pKa = 4.26FDD706 pKa = 4.76TIWEE710 pKa = 4.51INGSDD715 pKa = 3.44NNGYY719 pKa = 9.31PFLRR723 pKa = 11.84WQGYY727 pKa = 10.21DD728 pKa = 2.9GGQTNEE734 pKa = 4.27PPVLSAVGVSAVTQTTATLNFTSDD758 pKa = 2.94KK759 pKa = 10.88AGNYY763 pKa = 9.17YY764 pKa = 10.48FLVYY768 pKa = 10.41SAGDD772 pKa = 3.89DD773 pKa = 3.81APDD776 pKa = 3.59AATIKK781 pKa = 10.77AQGEE785 pKa = 4.0AAAKK789 pKa = 8.53GTGTAAAAANAAQVTGLTASTAYY812 pKa = 8.98KK813 pKa = 10.28AYY815 pKa = 10.8VIVEE819 pKa = 4.15DD820 pKa = 4.11AEE822 pKa = 4.47EE823 pKa = 4.48NISNVAVIGFTTATAEE839 pKa = 4.12MPIGADD845 pKa = 3.34ISPALCTFDD854 pKa = 5.86LEE856 pKa = 4.85NPDD859 pKa = 3.76NVSTTISWGSATTVTAVVYY878 pKa = 7.7GTTSLTTPADD888 pKa = 3.75YY889 pKa = 10.83NVSGNTLIITAGYY902 pKa = 10.52LEE904 pKa = 4.58GLNLSAGDD912 pKa = 3.65TVGFEE917 pKa = 3.86ISFDD921 pKa = 3.84VGDD924 pKa = 4.16SASLTVNIVNGYY936 pKa = 9.7IPGTDD941 pKa = 3.12ATLSDD946 pKa = 4.14LKK948 pKa = 11.36VGGVTVAGFVYY959 pKa = 10.62DD960 pKa = 3.61NYY962 pKa = 10.98DD963 pKa = 3.19YY964 pKa = 11.07SIQLPYY970 pKa = 9.55GTLPGSLAATVSATPNDD987 pKa = 3.95PKK989 pKa = 11.06AGVAITQAASLPGSATIVVTAEE1011 pKa = 3.9DD1012 pKa = 3.72TMTTQTYY1019 pKa = 7.92TVNFTLEE1026 pKa = 4.07AASNNAPNRR1035 pKa = 11.84KK1036 pKa = 7.47TGVPATTSASVTVNTAYY1053 pKa = 9.69TLDD1056 pKa = 3.75LSTIFEE1062 pKa = 4.91DD1063 pKa = 4.8ADD1065 pKa = 4.12SDD1067 pKa = 4.13PLTYY1071 pKa = 9.84TVSIDD1076 pKa = 3.39GANPVAAAANYY1087 pKa = 10.2VYY1089 pKa = 10.13TPTSAGDD1096 pKa = 3.49TILVFKK1102 pKa = 11.02ANDD1105 pKa = 3.64GTADD1109 pKa = 3.45STDD1112 pKa = 3.55TYY1114 pKa = 11.2TVTLTANAAPPQTYY1128 pKa = 10.75ALTITAGTGGSITTGSSGNYY1148 pKa = 9.17AAGTVISIAAAPSSGYY1164 pKa = 10.71SFSKK1168 pKa = 9.32WSSVGGGTFGSTTSASTTFTMPAGAVTITAGFTHH1202 pKa = 6.57NGSGGGNSGGGGSTTPSTPTYY1223 pKa = 9.96KK1224 pKa = 10.7ADD1226 pKa = 3.3VKK1228 pKa = 10.88AGNGSEE1234 pKa = 4.12TTLPVTVDD1242 pKa = 3.25KK1243 pKa = 11.32DD1244 pKa = 3.29AGTASIDD1251 pKa = 3.71ADD1253 pKa = 4.01SQSLAQGGTVITIPSIPDD1271 pKa = 2.92IDD1273 pKa = 4.2TYY1275 pKa = 11.69SLGIPVPDD1283 pKa = 4.84LSTTDD1288 pKa = 3.38VQGTLSFNTDD1298 pKa = 2.89AVSITVPSNMLTGVADD1314 pKa = 3.86ADD1316 pKa = 3.93GNRR1319 pKa = 11.84AQITIGQGDD1328 pKa = 4.15KK1329 pKa = 11.41SNLSEE1334 pKa = 4.22DD1335 pKa = 3.66VKK1337 pKa = 11.19DD1338 pKa = 4.0AVGDD1342 pKa = 3.78RR1343 pKa = 11.84PLVQLTLSIDD1353 pKa = 3.86GKK1355 pKa = 7.37QTNWNNPDD1363 pKa = 3.27APVTVSIPYY1372 pKa = 9.03TPTAAEE1378 pKa = 3.86LAAPEE1383 pKa = 5.09HH1384 pKa = 5.95IVVWYY1389 pKa = 10.22INGAGNAVSVPNGRR1403 pKa = 11.84YY1404 pKa = 9.77DD1405 pKa = 3.63PATGAVTFTTTHH1417 pKa = 5.99FSYY1420 pKa = 10.95YY1421 pKa = 10.46AIAYY1425 pKa = 6.1VHH1427 pKa = 6.13KK1428 pKa = 9.39TFDD1431 pKa = 4.05DD1432 pKa = 4.23LEE1434 pKa = 4.4SVAWAKK1440 pKa = 10.74EE1441 pKa = 4.21SIEE1444 pKa = 3.97VLASKK1449 pKa = 10.82GILEE1453 pKa = 5.28GISEE1457 pKa = 4.58TEE1459 pKa = 3.81YY1460 pKa = 11.18SPQEE1464 pKa = 4.29NITRR1468 pKa = 11.84ADD1470 pKa = 3.4FLFFLVRR1477 pKa = 11.84TLGVDD1482 pKa = 3.33AEE1484 pKa = 4.74VNGNFDD1490 pKa = 5.65DD1491 pKa = 4.21ISSDD1495 pKa = 2.85AYY1497 pKa = 9.37YY1498 pKa = 10.94NKK1500 pKa = 10.3EE1501 pKa = 3.35IVIAKK1506 pKa = 9.95KK1507 pKa = 10.65LGITGGTGDD1516 pKa = 4.32NKK1518 pKa = 10.21FSPDD1522 pKa = 3.21ACITRR1527 pKa = 11.84QDD1529 pKa = 3.51MMVLTEE1535 pKa = 3.82RR1536 pKa = 11.84ALRR1539 pKa = 11.84MLKK1542 pKa = 10.33KK1543 pKa = 10.68LEE1545 pKa = 4.27AQGSASDD1552 pKa = 3.92LVRR1555 pKa = 11.84FTDD1558 pKa = 3.32KK1559 pKa = 11.29SLVAAYY1565 pKa = 9.54AVNSVASVIKK1575 pKa = 10.12EE1576 pKa = 3.71GLIVGSGDD1584 pKa = 3.98KK1585 pKa = 10.56INPLGNTTRR1594 pKa = 11.84AEE1596 pKa = 4.05ATVFLYY1602 pKa = 9.89RR1603 pKa = 11.84VYY1605 pKa = 11.31NKK1607 pKa = 9.15YY1608 pKa = 9.11TGYY1611 pKa = 11.3

Molecular weight: 166.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y7RD59|A0A4Y7RD59_9FIRM Formate hydrogenlyase subunit 7 OS=Pelotomaculum schinkii OX=78350 GN=hycG_1 PE=4 SV=1
MM1 pKa = 6.95KK2 pKa = 10.11QRR4 pKa = 11.84RR5 pKa = 11.84TSLRR9 pKa = 11.84PMRR12 pKa = 11.84MKK14 pKa = 10.39RR15 pKa = 11.84VLIIAGIMATIARR28 pKa = 11.84IQVNGLQGG36 pKa = 3.14

Molecular weight: 4.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4132

0

4132

1213829

30

2778

293.8

32.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.635 ± 0.047

1.274 ± 0.021

5.027 ± 0.028

6.639 ± 0.044

3.868 ± 0.028

7.908 ± 0.047

1.725 ± 0.017

6.794 ± 0.035

5.794 ± 0.038

10.139 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.56 ± 0.018

4.025 ± 0.033

4.242 ± 0.029

3.393 ± 0.024

5.243 ± 0.039

5.699 ± 0.033

5.257 ± 0.044

7.423 ± 0.035

1.018 ± 0.015

3.339 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski