Amino acid dipepetide frequency for Gordonia phage Kenosha

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.204AlaAla: 9.204 ± 1.323
0.261AlaCys: 0.261 ± 0.12
5.125AlaAsp: 5.125 ± 0.47
6.327AlaGlu: 6.327 ± 0.581
3.504AlaPhe: 3.504 ± 0.462
6.589AlaGly: 6.589 ± 0.953
1.621AlaHis: 1.621 ± 0.348
5.805AlaIle: 5.805 ± 0.74
5.7AlaLys: 5.7 ± 0.536
6.589AlaLeu: 6.589 ± 1.094
2.824AlaMet: 2.824 ± 0.438
3.922AlaAsn: 3.922 ± 0.589
1.464AlaPro: 1.464 ± 0.307
2.719AlaGln: 2.719 ± 0.453
4.706AlaArg: 4.706 ± 0.427
4.916AlaSer: 4.916 ± 0.524
5.648AlaThr: 5.648 ± 0.771
7.06AlaVal: 7.06 ± 1.024
0.784AlaTrp: 0.784 ± 0.223
2.51AlaTyr: 2.51 ± 0.438
0.0AlaXaa: 0.0 ± 0.0
Cys
0.261CysAla: 0.261 ± 0.14
0.052CysCys: 0.052 ± 0.061
0.314CysAsp: 0.314 ± 0.123
0.366CysGlu: 0.366 ± 0.144
0.052CysPhe: 0.052 ± 0.052
0.732CysGly: 0.732 ± 0.235
0.105CysHis: 0.105 ± 0.077
0.418CysIle: 0.418 ± 0.163
0.261CysLys: 0.261 ± 0.126
0.732CysLeu: 0.732 ± 0.226
0.052CysMet: 0.052 ± 0.066
0.261CysAsn: 0.261 ± 0.106
0.261CysPro: 0.261 ± 0.128
0.261CysGln: 0.261 ± 0.131
0.418CysArg: 0.418 ± 0.196
0.575CysSer: 0.575 ± 0.199
0.314CysThr: 0.314 ± 0.151
0.471CysVal: 0.471 ± 0.163
0.314CysTrp: 0.314 ± 0.119
0.209CysTyr: 0.209 ± 0.103
0.0CysXaa: 0.0 ± 0.0
Asp
4.706AspAla: 4.706 ± 0.615
0.261AspCys: 0.261 ± 0.123
3.713AspAsp: 3.713 ± 0.584
5.543AspGlu: 5.543 ± 0.674
2.876AspPhe: 2.876 ± 0.402
4.759AspGly: 4.759 ± 0.47
1.255AspHis: 1.255 ± 0.276
3.765AspIle: 3.765 ± 0.465
3.242AspLys: 3.242 ± 0.557
4.654AspLeu: 4.654 ± 0.719
1.987AspMet: 1.987 ± 0.299
2.719AspAsn: 2.719 ± 0.369
3.138AspPro: 3.138 ± 0.424
2.615AspGln: 2.615 ± 0.352
3.451AspArg: 3.451 ± 0.446
3.294AspSer: 3.294 ± 0.414
2.876AspThr: 2.876 ± 0.368
3.87AspVal: 3.87 ± 0.436
0.837AspTrp: 0.837 ± 0.215
1.621AspTyr: 1.621 ± 0.377
0.0AspXaa: 0.0 ± 0.0
Glu
5.961GluAla: 5.961 ± 0.582
0.523GluCys: 0.523 ± 0.18
4.497GluAsp: 4.497 ± 0.56
4.968GluGlu: 4.968 ± 0.585
2.092GluPhe: 2.092 ± 0.375
5.072GluGly: 5.072 ± 0.55
1.36GluHis: 1.36 ± 0.24
4.079GluIle: 4.079 ± 0.425
5.125GluLys: 5.125 ± 0.652
7.112GluLeu: 7.112 ± 0.643
2.51GluMet: 2.51 ± 0.295
2.667GluAsn: 2.667 ± 0.352
2.301GluPro: 2.301 ± 0.453
2.928GluGln: 2.928 ± 0.426
3.085GluArg: 3.085 ± 0.553
3.765GluSer: 3.765 ± 0.36
2.981GluThr: 2.981 ± 0.407
4.863GluVal: 4.863 ± 0.425
1.464GluTrp: 1.464 ± 0.236
2.353GluTyr: 2.353 ± 0.363
0.0GluXaa: 0.0 ± 0.0
Phe
2.353PheAla: 2.353 ± 0.37
0.418PheCys: 0.418 ± 0.15
3.504PheAsp: 3.504 ± 0.436
3.033PheGlu: 3.033 ± 0.45
1.36PhePhe: 1.36 ± 0.28
2.562PheGly: 2.562 ± 0.413
0.418PheHis: 0.418 ± 0.126
1.621PheIle: 1.621 ± 0.259
1.935PheLys: 1.935 ± 0.362
2.981PheLeu: 2.981 ± 0.403
1.15PheMet: 1.15 ± 0.226
1.726PheAsn: 1.726 ± 0.289
1.516PhePro: 1.516 ± 0.319
0.784PheGln: 0.784 ± 0.224
1.883PheArg: 1.883 ± 0.297
2.092PheSer: 2.092 ± 0.328
1.987PheThr: 1.987 ± 0.35
2.092PheVal: 2.092 ± 0.39
0.366PheTrp: 0.366 ± 0.126
0.941PheTyr: 0.941 ± 0.234
0.0PheXaa: 0.0 ± 0.0
Gly
6.694GlyAla: 6.694 ± 0.88
0.418GlyCys: 0.418 ± 0.158
4.759GlyAsp: 4.759 ± 0.4
4.654GlyGlu: 4.654 ± 0.469
2.405GlyPhe: 2.405 ± 0.373
6.118GlyGly: 6.118 ± 0.804
1.36GlyHis: 1.36 ± 0.328
4.549GlyIle: 4.549 ± 0.846
4.131GlyLys: 4.131 ± 0.506
5.648GlyLeu: 5.648 ± 0.958
2.824GlyMet: 2.824 ± 0.402
3.033GlyAsn: 3.033 ± 0.513
2.876GlyPro: 2.876 ± 0.409
2.719GlyGln: 2.719 ± 0.505
3.19GlyArg: 3.19 ± 0.35
5.386GlySer: 5.386 ± 0.652
5.125GlyThr: 5.125 ± 0.428
6.275GlyVal: 6.275 ± 0.709
0.941GlyTrp: 0.941 ± 0.213
2.824GlyTyr: 2.824 ± 0.412
0.0GlyXaa: 0.0 ± 0.0
His
1.36HisAla: 1.36 ± 0.345
0.209HisCys: 0.209 ± 0.094
1.098HisAsp: 1.098 ± 0.275
1.621HisGlu: 1.621 ± 0.375
0.628HisPhe: 0.628 ± 0.194
1.569HisGly: 1.569 ± 0.294
0.523HisHis: 0.523 ± 0.144
1.883HisIle: 1.883 ± 0.41
0.837HisLys: 0.837 ± 0.207
1.464HisLeu: 1.464 ± 0.317
0.314HisMet: 0.314 ± 0.134
0.889HisAsn: 0.889 ± 0.228
0.994HisPro: 0.994 ± 0.252
0.68HisGln: 0.68 ± 0.161
1.412HisArg: 1.412 ± 0.291
1.15HisSer: 1.15 ± 0.258
0.68HisThr: 0.68 ± 0.185
1.255HisVal: 1.255 ± 0.259
0.157HisTrp: 0.157 ± 0.088
1.203HisTyr: 1.203 ± 0.256
0.0HisXaa: 0.0 ± 0.0
Ile
5.543IleAla: 5.543 ± 0.598
0.471IleCys: 0.471 ± 0.149
4.131IleAsp: 4.131 ± 0.438
4.027IleGlu: 4.027 ± 0.59
1.778IlePhe: 1.778 ± 0.284
4.654IleGly: 4.654 ± 0.684
1.36IleHis: 1.36 ± 0.291
2.458IleIle: 2.458 ± 0.437
3.085IleLys: 3.085 ± 0.33
4.916IleLeu: 4.916 ± 0.55
1.046IleMet: 1.046 ± 0.268
1.935IleAsn: 1.935 ± 0.31
2.196IlePro: 2.196 ± 0.299
1.883IleGln: 1.883 ± 0.344
2.824IleArg: 2.824 ± 0.372
3.242IleSer: 3.242 ± 0.47
3.817IleThr: 3.817 ± 0.474
4.706IleVal: 4.706 ± 0.504
0.575IleTrp: 0.575 ± 0.164
1.83IleTyr: 1.83 ± 0.337
0.0IleXaa: 0.0 ± 0.0
Lys
5.491LysAla: 5.491 ± 0.576
0.261LysCys: 0.261 ± 0.147
3.347LysAsp: 3.347 ± 0.525
4.445LysGlu: 4.445 ± 0.549
1.935LysPhe: 1.935 ± 0.396
4.288LysGly: 4.288 ± 0.583
1.621LysHis: 1.621 ± 0.341
3.085LysIle: 3.085 ± 0.416
3.661LysLys: 3.661 ± 0.665
5.072LysLeu: 5.072 ± 0.554
1.516LysMet: 1.516 ± 0.287
2.615LysAsn: 2.615 ± 0.305
2.824LysPro: 2.824 ± 0.503
2.196LysGln: 2.196 ± 0.256
2.667LysArg: 2.667 ± 0.432
3.87LysSer: 3.87 ± 0.381
3.19LysThr: 3.19 ± 0.335
4.811LysVal: 4.811 ± 0.546
1.098LysTrp: 1.098 ± 0.222
1.987LysTyr: 1.987 ± 0.361
0.0LysXaa: 0.0 ± 0.0
Leu
7.478LeuAla: 7.478 ± 1.258
0.575LeuCys: 0.575 ± 0.19
5.282LeuAsp: 5.282 ± 0.512
4.288LeuGlu: 4.288 ± 0.557
2.615LeuPhe: 2.615 ± 0.426
6.223LeuGly: 6.223 ± 0.992
1.15LeuHis: 1.15 ± 0.275
4.236LeuIle: 4.236 ± 0.473
5.648LeuLys: 5.648 ± 0.613
5.334LeuLeu: 5.334 ± 0.54
1.935LeuMet: 1.935 ± 0.324
4.759LeuAsn: 4.759 ± 0.522
2.667LeuPro: 2.667 ± 0.392
2.301LeuGln: 2.301 ± 0.312
3.87LeuArg: 3.87 ± 0.442
5.857LeuSer: 5.857 ± 0.587
6.746LeuThr: 6.746 ± 0.657
6.327LeuVal: 6.327 ± 0.54
1.098LeuTrp: 1.098 ± 0.218
2.824LeuTyr: 2.824 ± 0.531
0.0LeuXaa: 0.0 ± 0.0
Met
2.876MetAla: 2.876 ± 0.551
0.157MetCys: 0.157 ± 0.107
1.307MetAsp: 1.307 ± 0.294
1.516MetGlu: 1.516 ± 0.336
0.837MetPhe: 0.837 ± 0.187
1.987MetGly: 1.987 ± 0.294
0.523MetHis: 0.523 ± 0.141
1.778MetIle: 1.778 ± 0.279
1.673MetLys: 1.673 ± 0.268
2.615MetLeu: 2.615 ± 0.34
0.941MetMet: 0.941 ± 0.252
1.203MetAsn: 1.203 ± 0.235
1.255MetPro: 1.255 ± 0.267
0.889MetGln: 0.889 ± 0.192
1.36MetArg: 1.36 ± 0.267
2.353MetSer: 2.353 ± 0.396
2.405MetThr: 2.405 ± 0.289
1.673MetVal: 1.673 ± 0.278
0.157MetTrp: 0.157 ± 0.094
0.471MetTyr: 0.471 ± 0.136
0.0MetXaa: 0.0 ± 0.0
Asn
3.713AsnAla: 3.713 ± 0.482
0.314AsnCys: 0.314 ± 0.149
3.138AsnAsp: 3.138 ± 0.486
3.138AsnGlu: 3.138 ± 0.362
1.621AsnPhe: 1.621 ± 0.259
3.138AsnGly: 3.138 ± 0.451
1.15AsnHis: 1.15 ± 0.224
1.673AsnIle: 1.673 ± 0.281
2.301AsnLys: 2.301 ± 0.35
3.922AsnLeu: 3.922 ± 0.434
0.994AsnMet: 0.994 ± 0.292
1.778AsnAsn: 1.778 ± 0.336
2.981AsnPro: 2.981 ± 0.392
1.464AsnGln: 1.464 ± 0.211
2.876AsnArg: 2.876 ± 0.445
2.928AsnSer: 2.928 ± 0.371
3.033AsnThr: 3.033 ± 0.437
2.562AsnVal: 2.562 ± 0.393
0.366AsnTrp: 0.366 ± 0.131
1.098AsnTyr: 1.098 ± 0.25
0.0AsnXaa: 0.0 ± 0.0
Pro
3.294ProAla: 3.294 ± 0.423
0.418ProCys: 0.418 ± 0.162
2.772ProAsp: 2.772 ± 0.454
3.974ProGlu: 3.974 ± 0.478
1.778ProPhe: 1.778 ± 0.27
2.981ProGly: 2.981 ± 0.433
0.784ProHis: 0.784 ± 0.202
1.935ProIle: 1.935 ± 0.356
2.353ProLys: 2.353 ± 0.34
2.249ProLeu: 2.249 ± 0.331
1.203ProMet: 1.203 ± 0.204
2.092ProAsn: 2.092 ± 0.377
2.196ProPro: 2.196 ± 0.466
1.36ProGln: 1.36 ± 0.242
1.987ProArg: 1.987 ± 0.339
3.085ProSer: 3.085 ± 0.547
2.772ProThr: 2.772 ± 0.473
3.19ProVal: 3.19 ± 0.479
0.523ProTrp: 0.523 ± 0.157
1.464ProTyr: 1.464 ± 0.318
0.0ProXaa: 0.0 ± 0.0
Gln
3.451GlnAla: 3.451 ± 0.508
0.209GlnCys: 0.209 ± 0.122
1.36GlnAsp: 1.36 ± 0.274
1.726GlnGlu: 1.726 ± 0.3
0.994GlnPhe: 0.994 ± 0.217
2.405GlnGly: 2.405 ± 0.427
1.098GlnHis: 1.098 ± 0.255
1.935GlnIle: 1.935 ± 0.339
1.883GlnLys: 1.883 ± 0.287
3.87GlnLeu: 3.87 ± 0.397
0.837GlnMet: 0.837 ± 0.243
1.464GlnAsn: 1.464 ± 0.248
1.569GlnPro: 1.569 ± 0.409
1.778GlnGln: 1.778 ± 0.328
1.987GlnArg: 1.987 ± 0.303
1.935GlnSer: 1.935 ± 0.348
2.144GlnThr: 2.144 ± 0.326
2.51GlnVal: 2.51 ± 0.384
0.366GlnTrp: 0.366 ± 0.165
1.15GlnTyr: 1.15 ± 0.294
0.0GlnXaa: 0.0 ± 0.0
Arg
3.87ArgAla: 3.87 ± 0.545
0.314ArgCys: 0.314 ± 0.112
2.249ArgAsp: 2.249 ± 0.371
3.347ArgGlu: 3.347 ± 0.454
1.569ArgPhe: 1.569 ± 0.284
2.824ArgGly: 2.824 ± 0.462
1.307ArgHis: 1.307 ± 0.306
2.719ArgIle: 2.719 ± 0.326
3.713ArgLys: 3.713 ± 0.534
4.863ArgLeu: 4.863 ± 0.614
1.464ArgMet: 1.464 ± 0.264
2.458ArgAsn: 2.458 ± 0.328
2.981ArgPro: 2.981 ± 0.473
2.353ArgGln: 2.353 ± 0.402
3.033ArgArg: 3.033 ± 0.535
2.562ArgSer: 2.562 ± 0.417
3.451ArgThr: 3.451 ± 0.363
3.504ArgVal: 3.504 ± 0.457
0.575ArgTrp: 0.575 ± 0.215
1.516ArgTyr: 1.516 ± 0.248
0.0ArgXaa: 0.0 ± 0.0
Ser
4.183SerAla: 4.183 ± 0.501
0.314SerCys: 0.314 ± 0.155
4.079SerAsp: 4.079 ± 0.431
5.282SerGlu: 5.282 ± 0.464
1.883SerPhe: 1.883 ± 0.343
5.648SerGly: 5.648 ± 0.588
1.098SerHis: 1.098 ± 0.25
3.608SerIle: 3.608 ± 0.379
3.922SerLys: 3.922 ± 0.653
5.072SerLeu: 5.072 ± 0.521
1.935SerMet: 1.935 ± 0.284
3.085SerAsn: 3.085 ± 0.517
2.405SerPro: 2.405 ± 0.407
2.092SerGln: 2.092 ± 0.299
2.249SerArg: 2.249 ± 0.326
4.497SerSer: 4.497 ± 0.614
4.602SerThr: 4.602 ± 0.502
4.131SerVal: 4.131 ± 0.48
1.36SerTrp: 1.36 ± 0.285
2.458SerTyr: 2.458 ± 0.397
0.0SerXaa: 0.0 ± 0.0
Thr
6.171ThrAla: 6.171 ± 0.843
0.261ThrCys: 0.261 ± 0.129
3.294ThrAsp: 3.294 ± 0.363
3.817ThrGlu: 3.817 ± 0.394
2.353ThrPhe: 2.353 ± 0.307
5.386ThrGly: 5.386 ± 0.591
0.889ThrHis: 0.889 ± 0.228
4.445ThrIle: 4.445 ± 0.55
3.556ThrLys: 3.556 ± 0.445
5.386ThrLeu: 5.386 ± 0.484
1.726ThrMet: 1.726 ± 0.295
2.667ThrAsn: 2.667 ± 0.314
3.347ThrPro: 3.347 ± 0.447
1.83ThrGln: 1.83 ± 0.281
3.085ThrArg: 3.085 ± 0.426
4.131ThrSer: 4.131 ± 0.403
4.759ThrThr: 4.759 ± 0.552
5.072ThrVal: 5.072 ± 0.596
0.523ThrTrp: 0.523 ± 0.175
1.987ThrTyr: 1.987 ± 0.402
0.0ThrXaa: 0.0 ± 0.0
Val
6.484ValAla: 6.484 ± 0.593
0.628ValCys: 0.628 ± 0.195
4.393ValAsp: 4.393 ± 0.406
4.968ValGlu: 4.968 ± 0.501
2.667ValPhe: 2.667 ± 0.334
4.968ValGly: 4.968 ± 0.568
1.36ValHis: 1.36 ± 0.307
4.131ValIle: 4.131 ± 0.609
4.183ValLys: 4.183 ± 0.425
5.177ValLeu: 5.177 ± 0.516
1.883ValMet: 1.883 ± 0.274
2.562ValAsn: 2.562 ± 0.359
3.504ValPro: 3.504 ± 0.46
2.405ValGln: 2.405 ± 0.359
4.183ValArg: 4.183 ± 0.534
5.229ValSer: 5.229 ± 0.509
5.177ValThr: 5.177 ± 0.563
5.857ValVal: 5.857 ± 0.715
1.098ValTrp: 1.098 ± 0.265
2.458ValTyr: 2.458 ± 0.448
0.0ValXaa: 0.0 ± 0.0
Trp
1.098TrpAla: 1.098 ± 0.228
0.157TrpCys: 0.157 ± 0.104
0.732TrpAsp: 0.732 ± 0.221
1.15TrpGlu: 1.15 ± 0.215
0.471TrpPhe: 0.471 ± 0.184
1.046TrpGly: 1.046 ± 0.197
0.418TrpHis: 0.418 ± 0.145
0.471TrpIle: 0.471 ± 0.154
0.628TrpLys: 0.628 ± 0.169
1.046TrpLeu: 1.046 ± 0.285
0.209TrpMet: 0.209 ± 0.104
1.15TrpAsn: 1.15 ± 0.223
0.68TrpPro: 0.68 ± 0.217
0.209TrpGln: 0.209 ± 0.089
0.784TrpArg: 0.784 ± 0.189
0.732TrpSer: 0.732 ± 0.192
0.941TrpThr: 0.941 ± 0.224
0.784TrpVal: 0.784 ± 0.193
0.157TrpTrp: 0.157 ± 0.092
0.471TrpTyr: 0.471 ± 0.157
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.085TyrAla: 3.085 ± 0.577
0.209TyrCys: 0.209 ± 0.12
2.092TyrAsp: 2.092 ± 0.381
1.516TyrGlu: 1.516 ± 0.292
1.307TyrPhe: 1.307 ± 0.293
2.981TyrGly: 2.981 ± 0.379
0.523TyrHis: 0.523 ± 0.178
1.883TyrIle: 1.883 ± 0.426
2.144TyrLys: 2.144 ± 0.455
2.51TyrLeu: 2.51 ± 0.354
0.575TyrMet: 0.575 ± 0.216
1.203TyrAsn: 1.203 ± 0.232
1.255TyrPro: 1.255 ± 0.308
1.203TyrGln: 1.203 ± 0.235
1.621TyrArg: 1.621 ± 0.289
2.301TyrSer: 2.301 ± 0.387
2.092TyrThr: 2.092 ± 0.404
2.301TyrVal: 2.301 ± 0.281
0.575TyrTrp: 0.575 ± 0.184
0.628TyrTyr: 0.628 ± 0.232
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 90 proteins (19124 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski