Amino acid dipepetide frequency for Clostridium sp. Maddingley MBC34-26

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.201AlaAla: 4.201 ± 0.065	0.692AlaCys: 0.692 ± 0.023	2.847AlaAsp: 2.847 ± 0.05	3.617AlaGlu: 3.617 ± 0.05	2.573AlaPhe: 2.573 ± 0.046	3.884AlaGly: 3.884 ± 0.058	0.822AlaHis: 0.822 ± 0.024	5.748AlaIle: 5.748 ± 0.066	4.637AlaLys: 4.637 ± 0.062	5.782AlaLeu: 5.782 ± 0.061	1.657AlaMet: 1.657 ± 0.032	2.856AlaAsn: 2.856 ± 0.043	1.468AlaPro: 1.468 ± 0.031	1.59AlaGln: 1.59 ± 0.034	1.862AlaArg: 1.862 ± 0.034	3.576AlaSer: 3.576 ± 0.053	2.854AlaThr: 2.854 ± 0.049	3.954AlaVal: 3.954 ± 0.06	0.437AlaTrp: 0.437 ± 0.015	2.223AlaTyr: 2.223 ± 0.034	0.0AlaXaa: 0.0 ± 0.0
Cys	0.654CysAla: 0.654 ± 0.021	0.199CysCys: 0.199 ± 0.013	0.691CysAsp: 0.691 ± 0.022	0.795CysGlu: 0.795 ± 0.023	0.489CysPhe: 0.489 ± 0.021	1.101CysGly: 1.101 ± 0.027	0.217CysHis: 0.217 ± 0.012	1.176CysIle: 1.176 ± 0.029	0.941CysLys: 0.941 ± 0.023	0.894CysLeu: 0.894 ± 0.022	0.273CysMet: 0.273 ± 0.014	0.794CysAsn: 0.794 ± 0.023	0.47CysPro: 0.47 ± 0.018	0.222CysGln: 0.222 ± 0.012	0.424CysArg: 0.424 ± 0.015	0.859CysSer: 0.859 ± 0.023	0.618CysThr: 0.618 ± 0.017	0.645CysVal: 0.645 ± 0.019	0.087CysTrp: 0.087 ± 0.007	0.46CysTyr: 0.46 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	2.876AspAla: 2.876 ± 0.048	0.625AspCys: 0.625 ± 0.021	2.98AspAsp: 2.98 ± 0.046	4.681AspGlu: 4.681 ± 0.055	2.759AspPhe: 2.759 ± 0.039	3.652AspGly: 3.652 ± 0.059	0.549AspHis: 0.549 ± 0.02	5.934AspIle: 5.934 ± 0.071	5.318AspLys: 5.318 ± 0.057	4.869AspLeu: 4.869 ± 0.055	1.416AspMet: 1.416 ± 0.03	3.662AspAsn: 3.662 ± 0.049	1.361AspPro: 1.361 ± 0.034	0.941AspGln: 0.941 ± 0.024	1.699AspArg: 1.699 ± 0.031	3.368AspSer: 3.368 ± 0.047	2.564AspThr: 2.564 ± 0.041	3.4AspVal: 3.4 ± 0.042	0.447AspTrp: 0.447 ± 0.018	2.697AspTyr: 2.697 ± 0.043	0.0AspXaa: 0.0 ± 0.0
Glu	4.237GluAla: 4.237 ± 0.059	0.793GluCys: 0.793 ± 0.025	4.089GluAsp: 4.089 ± 0.046	6.731GluGlu: 6.731 ± 0.09	3.184GluPhe: 3.184 ± 0.042	3.908GluGly: 3.908 ± 0.051	1.003GluHis: 1.003 ± 0.023	7.421GluIle: 7.421 ± 0.085	7.409GluLys: 7.409 ± 0.08	6.904GluLeu: 6.904 ± 0.091	1.959GluMet: 1.959 ± 0.033	5.435GluAsn: 5.435 ± 0.065	1.378GluPro: 1.378 ± 0.032	1.942GluGln: 1.942 ± 0.039	2.592GluArg: 2.592 ± 0.036	3.612GluSer: 3.612 ± 0.054	2.986GluThr: 2.986 ± 0.044	4.703GluVal: 4.703 ± 0.057	0.545GluTrp: 0.545 ± 0.018	3.152GluTyr: 3.152 ± 0.046	0.0GluXaa: 0.0 ± 0.0
Phe	2.316PheAla: 2.316 ± 0.042	0.555PheCys: 0.555 ± 0.02	2.634PheAsp: 2.634 ± 0.039	2.925PheGlu: 2.925 ± 0.041	1.987PhePhe: 1.987 ± 0.037	2.931PheGly: 2.931 ± 0.046	0.586PheHis: 0.586 ± 0.02	4.453PheIle: 4.453 ± 0.055	3.647PheLys: 3.647 ± 0.05	3.882PheLeu: 3.882 ± 0.057	1.119PheMet: 1.119 ± 0.026	3.033PheAsn: 3.033 ± 0.04	1.178PhePro: 1.178 ± 0.028	0.974PheGln: 0.974 ± 0.023	1.333PheArg: 1.333 ± 0.031	3.282PheSer: 3.282 ± 0.045	2.315PheThr: 2.315 ± 0.035	2.522PheVal: 2.522 ± 0.044	0.344PheTrp: 0.344 ± 0.014	1.883PheTyr: 1.883 ± 0.038	0.0PheXaa: 0.0 ± 0.0
Gly	4.017GlyAla: 4.017 ± 0.059	0.955GlyCys: 0.955 ± 0.028	3.219GlyAsp: 3.219 ± 0.048	4.149GlyGlu: 4.149 ± 0.05	2.991GlyPhe: 2.991 ± 0.039	4.091GlyGly: 4.091 ± 0.068	0.977GlyHis: 0.977 ± 0.025	6.808GlyIle: 6.808 ± 0.068	5.281GlyLys: 5.281 ± 0.053	5.163GlyLeu: 5.163 ± 0.058	1.721GlyMet: 1.721 ± 0.034	3.448GlyAsn: 3.448 ± 0.065	1.164GlyPro: 1.164 ± 0.028	1.539GlyGln: 1.539 ± 0.034	2.004GlyArg: 2.004 ± 0.038	3.779GlySer: 3.779 ± 0.056	3.555GlyThr: 3.555 ± 0.054	4.322GlyVal: 4.322 ± 0.048	0.715GlyTrp: 0.715 ± 0.03	3.036GlyTyr: 3.036 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	0.705HisAla: 0.705 ± 0.024	0.232HisCys: 0.232 ± 0.011	0.752HisAsp: 0.752 ± 0.021	0.931HisGlu: 0.931 ± 0.023	0.629HisPhe: 0.629 ± 0.017	0.991HisGly: 0.991 ± 0.027	0.283HisHis: 0.283 ± 0.015	1.304HisIle: 1.304 ± 0.027	1.129HisLys: 1.129 ± 0.025	1.224HisLeu: 1.224 ± 0.025	0.344HisMet: 0.344 ± 0.016	0.805HisAsn: 0.805 ± 0.021	0.578HisPro: 0.578 ± 0.02	0.331HisGln: 0.331 ± 0.014	0.507HisArg: 0.507 ± 0.018	0.85HisSer: 0.85 ± 0.021	0.655HisThr: 0.655 ± 0.02	0.709HisVal: 0.709 ± 0.024	0.131HisTrp: 0.131 ± 0.008	0.567HisTyr: 0.567 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	5.784IleAla: 5.784 ± 0.067	1.274IleCys: 1.274 ± 0.026	5.765IleAsp: 5.765 ± 0.063	7.204IleGlu: 7.204 ± 0.074	4.184IlePhe: 4.184 ± 0.062	6.059IleGly: 6.059 ± 0.069	1.244IleHis: 1.244 ± 0.024	9.59IleIle: 9.59 ± 0.102	8.729IleLys: 8.729 ± 0.079	9.065IleLeu: 9.065 ± 0.09	2.284IleMet: 2.284 ± 0.039	6.771IleAsn: 6.771 ± 0.082	3.316IlePro: 3.316 ± 0.049	2.401IleGln: 2.401 ± 0.041	3.013IleArg: 3.013 ± 0.046	7.273IleSer: 7.273 ± 0.08	5.099IleThr: 5.099 ± 0.059	5.918IleVal: 5.918 ± 0.065	0.596IleTrp: 0.596 ± 0.019	3.594IleTyr: 3.594 ± 0.049	0.0IleXaa: 0.0 ± 0.0
Lys	4.867LysAla: 4.867 ± 0.061	0.886LysCys: 0.886 ± 0.023	5.682LysAsp: 5.682 ± 0.07	8.36LysGlu: 8.36 ± 0.084	3.204LysPhe: 3.204 ± 0.043	4.731LysGly: 4.731 ± 0.061	1.139LysHis: 1.139 ± 0.024	8.258LysIle: 8.258 ± 0.077	7.525LysLys: 7.525 ± 0.075	7.64LysLeu: 7.64 ± 0.074	2.311LysMet: 2.311 ± 0.035	6.243LysAsn: 6.243 ± 0.072	2.057LysPro: 2.057 ± 0.04	2.349LysGln: 2.349 ± 0.038	2.891LysArg: 2.891 ± 0.04	5.162LysSer: 5.162 ± 0.058	3.97LysThr: 3.97 ± 0.048	5.741LysVal: 5.741 ± 0.06	0.696LysTrp: 0.696 ± 0.021	4.097LysTyr: 4.097 ± 0.057	0.0LysXaa: 0.0 ± 0.0
Leu	5.219LeuAla: 5.219 ± 0.066	1.089LeuCys: 1.089 ± 0.023	5.017LeuAsp: 5.017 ± 0.057	6.416LeuGlu: 6.416 ± 0.072	3.738LeuPhe: 3.738 ± 0.059	5.806LeuGly: 5.806 ± 0.073	1.156LeuHis: 1.156 ± 0.026	8.469LeuIle: 8.469 ± 0.085	8.36LeuLys: 8.36 ± 0.077	7.887LeuLeu: 7.887 ± 0.085	2.264LeuMet: 2.264 ± 0.036	6.14LeuAsn: 6.14 ± 0.059	2.692LeuPro: 2.692 ± 0.04	2.225LeuGln: 2.225 ± 0.039	3.071LeuArg: 3.071 ± 0.043	6.675LeuSer: 6.675 ± 0.068	4.45LeuThr: 4.45 ± 0.048	5.194LeuVal: 5.194 ± 0.056	0.63LeuTrp: 0.63 ± 0.019	3.278LeuTyr: 3.278 ± 0.045	0.0LeuXaa: 0.0 ± 0.0
Met	1.707MetAla: 1.707 ± 0.038	0.274MetCys: 0.274 ± 0.014	1.447MetAsp: 1.447 ± 0.031	1.82MetGlu: 1.82 ± 0.034	0.962MetPhe: 0.962 ± 0.023	1.609MetGly: 1.609 ± 0.035	0.353MetHis: 0.353 ± 0.018	2.207MetIle: 2.207 ± 0.039	2.455MetLys: 2.455 ± 0.04	2.226MetLeu: 2.226 ± 0.034	0.696MetMet: 0.696 ± 0.024	1.757MetAsn: 1.757 ± 0.035	0.928MetPro: 0.928 ± 0.018	0.722MetGln: 0.722 ± 0.02	0.852MetArg: 0.852 ± 0.024	1.758MetSer: 1.758 ± 0.032	1.192MetThr: 1.192 ± 0.027	1.508MetVal: 1.508 ± 0.032	0.171MetTrp: 0.171 ± 0.011	0.855MetTyr: 0.855 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	3.244AsnAla: 3.244 ± 0.049	0.782AsnCys: 0.782 ± 0.024	3.437AsnAsp: 3.437 ± 0.048	4.744AsnGlu: 4.744 ± 0.069	2.718AsnPhe: 2.718 ± 0.043	4.003AsnGly: 4.003 ± 0.063	0.849AsnHis: 0.849 ± 0.022	7.159AsnIle: 7.159 ± 0.072	6.089AsnLys: 6.089 ± 0.061	5.834AsnLeu: 5.834 ± 0.063	1.7AsnMet: 1.7 ± 0.031	4.925AsnAsn: 4.925 ± 0.073	2.135AsnPro: 2.135 ± 0.037	1.561AsnGln: 1.561 ± 0.035	2.01AsnArg: 2.01 ± 0.038	4.585AsnSer: 4.585 ± 0.065	3.146AsnThr: 3.146 ± 0.048	3.76AsnVal: 3.76 ± 0.047	0.557AsnTrp: 0.557 ± 0.022	2.922AsnTyr: 2.922 ± 0.048	0.0AsnXaa: 0.0 ± 0.0
Pro	1.476ProAla: 1.476 ± 0.035	0.306ProCys: 0.306 ± 0.015	1.507ProAsp: 1.507 ± 0.029	2.17ProGlu: 2.17 ± 0.04	1.395ProPhe: 1.395 ± 0.028	1.626ProGly: 1.626 ± 0.032	0.453ProHis: 0.453 ± 0.016	2.705ProIle: 2.705 ± 0.046	2.191ProLys: 2.191 ± 0.035	2.519ProLeu: 2.519 ± 0.035	0.694ProMet: 0.694 ± 0.021	1.711ProAsn: 1.711 ± 0.034	0.624ProPro: 0.624 ± 0.022	0.828ProGln: 0.828 ± 0.023	0.791ProArg: 0.791 ± 0.024	1.785ProSer: 1.785 ± 0.036	1.414ProThr: 1.414 ± 0.031	1.923ProVal: 1.923 ± 0.033	0.274ProTrp: 0.274 ± 0.012	1.207ProTyr: 1.207 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	1.509GlnAla: 1.509 ± 0.034	0.276GlnCys: 0.276 ± 0.012	1.364GlnAsp: 1.364 ± 0.029	1.836GlnGlu: 1.836 ± 0.032	1.019GlnPhe: 1.019 ± 0.022	1.64GlnGly: 1.64 ± 0.036	0.321GlnHis: 0.321 ± 0.013	2.276GlnIle: 2.276 ± 0.038	2.136GlnLys: 2.136 ± 0.038	2.201GlnLeu: 2.201 ± 0.037	0.659GlnMet: 0.659 ± 0.021	1.709GlnAsn: 1.709 ± 0.032	0.58GlnPro: 0.58 ± 0.021	0.743GlnGln: 0.743 ± 0.024	0.943GlnArg: 0.943 ± 0.027	1.538GlnSer: 1.538 ± 0.032	1.238GlnThr: 1.238 ± 0.026	1.6GlnVal: 1.6 ± 0.033	0.235GlnTrp: 0.235 ± 0.012	1.117GlnTyr: 1.117 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	1.782ArgAla: 1.782 ± 0.038	0.379ArgCys: 0.379 ± 0.015	1.839ArgAsp: 1.839 ± 0.032	2.716ArgGlu: 2.716 ± 0.048	1.416ArgPhe: 1.416 ± 0.03	1.869ArgGly: 1.869 ± 0.039	0.464ArgHis: 0.464 ± 0.017	3.18ArgIle: 3.18 ± 0.047	2.957ArgLys: 2.957 ± 0.042	2.884ArgLeu: 2.884 ± 0.043	0.884ArgMet: 0.884 ± 0.022	2.133ArgAsn: 2.133 ± 0.032	0.808ArgPro: 0.808 ± 0.022	0.884ArgGln: 0.884 ± 0.026	1.288ArgArg: 1.288 ± 0.029	1.627ArgSer: 1.627 ± 0.027	1.58ArgThr: 1.58 ± 0.036	2.035ArgVal: 2.035 ± 0.04	0.272ArgTrp: 0.272 ± 0.012	1.439ArgTyr: 1.439 ± 0.029	0.0ArgXaa: 0.0 ± 0.0
Ser	3.468SerAla: 3.468 ± 0.045	0.698SerCys: 0.698 ± 0.024	3.464SerAsp: 3.464 ± 0.047	4.392SerGlu: 4.392 ± 0.046	3.098SerPhe: 3.098 ± 0.046	4.416SerGly: 4.416 ± 0.067	0.932SerHis: 0.932 ± 0.021	6.714SerIle: 6.714 ± 0.074	5.848SerLys: 5.848 ± 0.068	5.982SerLeu: 5.982 ± 0.066	1.693SerMet: 1.693 ± 0.031	4.292SerAsn: 4.292 ± 0.06	1.664SerPro: 1.664 ± 0.031	1.71SerGln: 1.71 ± 0.032	2.041SerArg: 2.041 ± 0.035	4.718SerSer: 4.718 ± 0.079	3.368SerThr: 3.368 ± 0.053	3.8SerVal: 3.8 ± 0.045	0.526SerTrp: 0.526 ± 0.019	2.637SerTyr: 2.637 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	3.038ThrAla: 3.038 ± 0.05	0.542ThrCys: 0.542 ± 0.018	2.451ThrAsp: 2.451 ± 0.044	2.91ThrGlu: 2.91 ± 0.043	2.272ThrPhe: 2.272 ± 0.035	3.631ThrGly: 3.631 ± 0.058	0.751ThrHis: 0.751 ± 0.023	4.885ThrIle: 4.885 ± 0.057	3.763ThrLys: 3.763 ± 0.046	4.774ThrLeu: 4.774 ± 0.05	1.109ThrMet: 1.109 ± 0.025	2.971ThrAsn: 2.971 ± 0.043	1.821ThrPro: 1.821 ± 0.034	1.315ThrGln: 1.315 ± 0.033	1.425ThrArg: 1.425 ± 0.03	3.346ThrSer: 3.346 ± 0.055	2.778ThrThr: 2.778 ± 0.049	3.288ThrVal: 3.288 ± 0.053	0.416ThrTrp: 0.416 ± 0.018	1.989ThrTyr: 1.989 ± 0.041	0.0ThrXaa: 0.0 ± 0.0
Val	3.72ValAla: 3.72 ± 0.057	0.789ValCys: 0.789 ± 0.022	3.635ValAsp: 3.635 ± 0.047	4.119ValGlu: 4.119 ± 0.058	2.795ValPhe: 2.795 ± 0.045	3.876ValGly: 3.876 ± 0.051	0.827ValHis: 0.827 ± 0.025	6.11ValIle: 6.11 ± 0.061	5.106ValLys: 5.106 ± 0.058	5.552ValLeu: 5.552 ± 0.06	1.528ValMet: 1.528 ± 0.033	3.929ValAsn: 3.929 ± 0.05	1.927ValPro: 1.927 ± 0.036	1.56ValGln: 1.56 ± 0.03	1.926ValArg: 1.926 ± 0.035	4.317ValSer: 4.317 ± 0.055	3.247ValThr: 3.247 ± 0.048	4.156ValVal: 4.156 ± 0.058	0.448ValTrp: 0.448 ± 0.015	2.423ValTyr: 2.423 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	0.419TrpAla: 0.419 ± 0.016	0.106TrpCys: 0.106 ± 0.008	0.443TrpAsp: 0.443 ± 0.018	0.482TrpGlu: 0.482 ± 0.02	0.363TrpPhe: 0.363 ± 0.015	0.564TrpGly: 0.564 ± 0.019	0.142TrpHis: 0.142 ± 0.01	0.769TrpIle: 0.769 ± 0.022	0.6TrpLys: 0.6 ± 0.019	0.673TrpLeu: 0.673 ± 0.019	0.225TrpMet: 0.225 ± 0.012	0.565TrpAsn: 0.565 ± 0.015	0.187TrpPro: 0.187 ± 0.011	0.237TrpGln: 0.237 ± 0.012	0.28TrpArg: 0.28 ± 0.012	0.47TrpSer: 0.47 ± 0.018	0.39TrpThr: 0.39 ± 0.018	0.467TrpVal: 0.467 ± 0.017	0.12TrpTrp: 0.12 ± 0.009	0.482TrpTyr: 0.482 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.039TyrAla: 2.039 ± 0.039	0.562TyrCys: 0.562 ± 0.017	2.534TyrAsp: 2.534 ± 0.067	2.986TyrGlu: 2.986 ± 0.046	2.124TyrPhe: 2.124 ± 0.04	2.547TyrGly: 2.547 ± 0.041	0.591TyrHis: 0.591 ± 0.021	3.997TyrIle: 3.997 ± 0.047	3.689TyrLys: 3.689 ± 0.058	3.818TyrLeu: 3.818 ± 0.055	0.963TyrMet: 0.963 ± 0.023	2.977TyrAsn: 2.977 ± 0.047	1.263TyrPro: 1.263 ± 0.029	0.865TyrGln: 0.865 ± 0.023	1.484TyrArg: 1.484 ± 0.026	2.881TyrSer: 2.881 ± 0.042	2.057TyrThr: 2.057 ± 0.035	2.345TyrVal: 2.345 ± 0.036	0.337TyrTrp: 0.337 ± 0.016	2.097TyrTyr: 2.097 ± 0.047	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5778 proteins (1727297 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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