Clostridium sp. CAG:1000

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1142 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5A384|R5A384_9CLOT SpoIIIAH OS=Clostridium sp. CAG:1000 OX=1262768 GN=BN451_00726 PE=4 SV=1
MM1 pKa = 7.67IKK3 pKa = 10.39YY4 pKa = 9.84KK5 pKa = 10.48YY6 pKa = 9.57DD7 pKa = 3.39SEE9 pKa = 4.9VIFDD13 pKa = 4.25ISNLDD18 pKa = 3.36KK19 pKa = 11.58SEE21 pKa = 4.68LSLLYY26 pKa = 10.47DD27 pKa = 5.05AIDD30 pKa = 4.22DD31 pKa = 3.88NKK33 pKa = 10.86HH34 pKa = 5.95YY35 pKa = 10.73IDD37 pKa = 4.29IEE39 pKa = 4.21MFFIGDD45 pKa = 3.7SNIPNFGPDD54 pKa = 2.91YY55 pKa = 11.07NYY57 pKa = 10.31IVNDD61 pKa = 3.13NKK63 pKa = 11.07LILKK67 pKa = 9.4YY68 pKa = 10.56IFF70 pKa = 4.11

Molecular weight: 8.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4ZZF3|R4ZZF3_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:1000 OX=1262768 GN=BN451_00552 PE=3 SV=1
MM1 pKa = 7.81ANARR5 pKa = 11.84NNARR9 pKa = 11.84SNARR13 pKa = 11.84KK14 pKa = 9.57NSCKK18 pKa = 10.31AKK20 pKa = 10.62SNARR24 pKa = 11.84SNSRR28 pKa = 11.84SRR30 pKa = 11.84STSNSRR36 pKa = 11.84SRR38 pKa = 3.44

Molecular weight: 4.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1142

0

1142

342649

30

2442

300.0

34.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.09 ± 0.06

1.195 ± 0.03

6.146 ± 0.058

6.982 ± 0.079

4.353 ± 0.057

5.211 ± 0.063

1.216 ± 0.023

10.165 ± 0.095

10.344 ± 0.083

8.889 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.391 ± 0.032

7.757 ± 0.089

2.343 ± 0.038

1.842 ± 0.03

2.989 ± 0.044

6.838 ± 0.061

5.355 ± 0.062

6.009 ± 0.058

0.522 ± 0.02

5.363 ± 0.079
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski