Nodularia spumigena CCY9414

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Aphanizomenonaceae; Nodularia; Nodularia spumigena

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5211 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0ZCV3|A0ZCV3_NODSP Primosomal protein N' OS=Nodularia spumigena CCY9414 OX=313624 GN=priA PE=3 SV=1
MM1 pKa = 7.61LGNTTSPLIGNLTATNTFNQIPGAVEE27 pKa = 3.39PVNYY31 pKa = 10.59YY32 pKa = 10.07EE33 pKa = 5.23FSVFDD38 pKa = 3.28TSRR41 pKa = 11.84ISLVLSGITQNSAQASIIYY60 pKa = 9.7DD61 pKa = 3.5RR62 pKa = 11.84NNNRR66 pKa = 11.84LLDD69 pKa = 4.02SGEE72 pKa = 3.96RR73 pKa = 11.84LYY75 pKa = 11.35SQTAFVDD82 pKa = 3.26INGTINPTLGAGNYY96 pKa = 9.19LVEE99 pKa = 4.23IQRR102 pKa = 11.84FNSPNNNNYY111 pKa = 10.22SGYY114 pKa = 10.13SVQLSGTPTPASILSDD130 pKa = 3.42PGNTLNSAYY139 pKa = 10.8NIGNFNGTRR148 pKa = 11.84TFKK151 pKa = 10.86EE152 pKa = 4.01FVGVVDD158 pKa = 3.77PVDD161 pKa = 3.51YY162 pKa = 11.43YY163 pKa = 11.42KK164 pKa = 11.01FSLADD169 pKa = 3.3TSEE172 pKa = 3.85ISLLLSEE179 pKa = 4.48LTEE182 pKa = 4.31NALKK186 pKa = 10.19TSIISDD192 pKa = 3.64ANNNGLIDD200 pKa = 4.0SGEE203 pKa = 3.94SRR205 pKa = 11.84YY206 pKa = 8.67TDD208 pKa = 3.17TAQVNSNGTINANLAAGNYY227 pKa = 9.35LIAVEE232 pKa = 3.95QDD234 pKa = 3.36SANTNSNYY242 pKa = 10.78SLTLSNLTISTPKK255 pKa = 9.78AAKK258 pKa = 10.2DD259 pKa = 3.69FNQDD263 pKa = 2.89NQTDD267 pKa = 3.57ILLTKK272 pKa = 10.26PSEE275 pKa = 4.37GLNKK279 pKa = 9.84AWLMDD284 pKa = 3.17GTNYY288 pKa = 9.69VGEE291 pKa = 4.28MNLPGLAAYY300 pKa = 9.99RR301 pKa = 11.84PVATPDD307 pKa = 3.3FNKK310 pKa = 10.79DD311 pKa = 3.02GNTDD315 pKa = 4.07LLVSNPNNGWNLVWFLDD332 pKa = 3.47GMNYY336 pKa = 9.94VGGVGLPIAAGWEE349 pKa = 4.07IKK351 pKa = 10.32GAADD355 pKa = 3.9FNGDD359 pKa = 3.6GNVDD363 pKa = 3.01ILLNNTANNWNTVWFLGGDD382 pKa = 3.25NGATYY387 pKa = 9.63TGYY390 pKa = 11.51GNLPVAEE397 pKa = 4.69GWDD400 pKa = 3.41ITGVADD406 pKa = 4.45FNGDD410 pKa = 3.24GKK412 pKa = 11.14ADD414 pKa = 3.92LLLNNPTEE422 pKa = 4.32GWNSVWFLDD431 pKa = 3.55GTDD434 pKa = 3.61YY435 pKa = 10.87IGYY438 pKa = 9.16EE439 pKa = 4.26NLPSSPGWQSLGTGDD454 pKa = 4.36FNSDD458 pKa = 3.56GKK460 pKa = 10.19PDD462 pKa = 3.85IIMNNPTQGWNSIWLMDD479 pKa = 3.58GTNYY483 pKa = 10.33TGFASLPTTPDD494 pKa = 2.48GWEE497 pKa = 3.86IAGMAA502 pKa = 4.09

Molecular weight: 53.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W6FP36|W6FP36_NODSP Uncharacterized protein OS=Nodularia spumigena CCY9414 OX=313624 GN=NSP_24780 PE=4 SV=1
MM1 pKa = 7.43TKK3 pKa = 10.56LGTSKK8 pKa = 10.45QKK10 pKa = 10.64NPQFVVGVRR19 pKa = 11.84TVVRR23 pKa = 11.84NPPLSFNLRR32 pKa = 11.84WVKK35 pKa = 10.61RR36 pKa = 11.84SLSPTTIFGG45 pKa = 3.52

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5211

0

5211

1456987

17

4171

279.6

31.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.902 ± 0.045

0.994 ± 0.013

4.773 ± 0.025

6.344 ± 0.036

3.976 ± 0.024

6.585 ± 0.039

1.91 ± 0.018

6.919 ± 0.031

4.846 ± 0.033

10.981 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.933 ± 0.016

4.473 ± 0.027

4.81 ± 0.028

5.553 ± 0.033

4.99 ± 0.026

6.321 ± 0.028

5.663 ± 0.032

6.559 ± 0.032

1.416 ± 0.017

3.052 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski