Tobacco mottle leaf curl virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B2ZWE7|B2ZWE7_9GEMI AC4 protein OS=Tobacco mottle leaf curl virus OX=530536 GN=AC4 PE=3 SV=1
MM1 pKa = 7.64PSVKK5 pKa = 10.02KK6 pKa = 10.46FRR8 pKa = 11.84VQSRR12 pKa = 11.84NYY14 pKa = 9.52FLTYY18 pKa = 8.6PQCSLSKK25 pKa = 11.0EE26 pKa = 4.09EE27 pKa = 5.81ALSQLQNLDD36 pKa = 3.35TPVNKK41 pKa = 10.11KK42 pKa = 9.98FIKK45 pKa = 9.67ICRR48 pKa = 11.84EE49 pKa = 3.52LHH51 pKa = 6.26EE52 pKa = 4.93NGEE55 pKa = 4.29PHH57 pKa = 6.86LHH59 pKa = 5.98VLVQFEE65 pKa = 4.35GKK67 pKa = 9.34YY68 pKa = 9.62VCTNYY73 pKa = 10.47RR74 pKa = 11.84FFDD77 pKa = 3.75LVSPSRR83 pKa = 11.84SAHH86 pKa = 5.01FHH88 pKa = 6.64PNVQGAKK95 pKa = 9.76SSSDD99 pKa = 3.11VKK101 pKa = 11.24SYY103 pKa = 10.79IDD105 pKa = 3.7KK106 pKa = 11.33DD107 pKa = 3.49GDD109 pKa = 3.67TLEE112 pKa = 4.1WGEE115 pKa = 3.95FQVDD119 pKa = 3.21GRR121 pKa = 11.84SARR124 pKa = 11.84GGCQSANDD132 pKa = 4.17TYY134 pKa = 11.77AKK136 pKa = 10.57ALNASSAEE144 pKa = 4.0EE145 pKa = 3.51ALQIIKK151 pKa = 10.28EE152 pKa = 4.13EE153 pKa = 4.13QPQHH157 pKa = 6.73FFLQHH162 pKa = 6.07HH163 pKa = 6.14NLVANAQRR171 pKa = 11.84IFQKK175 pKa = 10.74APEE178 pKa = 4.11PWVPPFQLSSLTNVPEE194 pKa = 4.61EE195 pKa = 4.01MQEE198 pKa = 3.79WADD201 pKa = 3.73EE202 pKa = 4.25YY203 pKa = 11.01FGRR206 pKa = 11.84GSAARR211 pKa = 11.84PEE213 pKa = 4.21RR214 pKa = 11.84PMSIIVEE221 pKa = 4.27GDD223 pKa = 3.04SRR225 pKa = 11.84TGKK228 pKa = 8.49TMWACSKK235 pKa = 9.46TMWARR240 pKa = 11.84ALGPHH245 pKa = 6.63NYY247 pKa = 10.14LSGHH251 pKa = 6.96LDD253 pKa = 3.47FNHH256 pKa = 6.23RR257 pKa = 11.84VYY259 pKa = 11.3SNEE262 pKa = 3.44VEE264 pKa = 4.32YY265 pKa = 11.37NVIDD269 pKa = 4.4DD270 pKa = 4.14VAPHH274 pKa = 5.29YY275 pKa = 10.97LKK277 pKa = 10.78LKK279 pKa = 9.14HH280 pKa = 5.88WKK282 pKa = 9.55EE283 pKa = 3.9LLGSQKK289 pKa = 10.72DD290 pKa = 3.48WQSNCKK296 pKa = 9.38YY297 pKa = 10.21GKK299 pKa = 9.18PVQIKK304 pKa = 9.93GGIPAIVLCNPGEE317 pKa = 4.16GASYY321 pKa = 11.29KK322 pKa = 10.87DD323 pKa = 3.72FLDD326 pKa = 3.9KK327 pKa = 11.36EE328 pKa = 4.72EE329 pKa = 4.4NSSLRR334 pKa = 11.84NFGDD338 pKa = 3.51RR339 pKa = 11.84CGSSPPAPLYY349 pKa = 10.65QEE351 pKa = 4.48GTQASQRR358 pKa = 11.84GGPSGGDD365 pKa = 3.25ALII368 pKa = 4.53

Molecular weight: 41.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B2ZWE4|B2ZWE4_9GEMI Replication enhancer OS=Tobacco mottle leaf curl virus OX=530536 GN=AC3 PE=3 SV=1
MM1 pKa = 8.04PKK3 pKa = 9.63RR4 pKa = 11.84DD5 pKa = 4.08LPWRR9 pKa = 11.84PYY11 pKa = 11.06AKK13 pKa = 9.39ISKK16 pKa = 9.56VSRR19 pKa = 11.84NANYY23 pKa = 10.37SPRR26 pKa = 11.84TRR28 pKa = 11.84VGPIVDD34 pKa = 4.98KK35 pKa = 10.94AAEE38 pKa = 4.1WVNRR42 pKa = 11.84PMYY45 pKa = 9.79RR46 pKa = 11.84KK47 pKa = 8.63PRR49 pKa = 11.84IYY51 pKa = 9.74RR52 pKa = 11.84TVRR55 pKa = 11.84TPDD58 pKa = 3.1VPRR61 pKa = 11.84GCEE64 pKa = 4.34GPCKK68 pKa = 9.32VQSYY72 pKa = 6.94EE73 pKa = 3.74QRR75 pKa = 11.84HH76 pKa = 6.38DD77 pKa = 3.16ISHH80 pKa = 6.13VGKK83 pKa = 10.55VMCISDD89 pKa = 3.56VTRR92 pKa = 11.84GNGITHH98 pKa = 6.78RR99 pKa = 11.84VGKK102 pKa = 9.44RR103 pKa = 11.84FCVKK107 pKa = 9.92SVYY110 pKa = 10.25ILGKK114 pKa = 9.79IGKK117 pKa = 8.34IWMDD121 pKa = 3.43EE122 pKa = 4.01NIKK125 pKa = 10.61LKK127 pKa = 10.73NHH129 pKa = 5.95TNSVMFWLVRR139 pKa = 11.84DD140 pKa = 3.7RR141 pKa = 11.84RR142 pKa = 11.84PYY144 pKa = 8.05GTPMDD149 pKa = 4.6FGQVFNMFDD158 pKa = 3.99NEE160 pKa = 4.11PSTATVKK167 pKa = 10.81NDD169 pKa = 2.84LRR171 pKa = 11.84DD172 pKa = 3.6RR173 pKa = 11.84YY174 pKa = 9.91QVMHH178 pKa = 7.14RR179 pKa = 11.84FHH181 pKa = 7.1AKK183 pKa = 8.01VTGGQYY189 pKa = 11.07ASNEE193 pKa = 3.78QAIVRR198 pKa = 11.84RR199 pKa = 11.84FWKK202 pKa = 10.31VNNHH206 pKa = 4.48VVYY209 pKa = 10.67NHH211 pKa = 5.8QEE213 pKa = 3.47AGKK216 pKa = 10.41YY217 pKa = 8.39EE218 pKa = 4.09NHH220 pKa = 6.52TEE222 pKa = 3.99NALLLYY228 pKa = 7.29MACTHH233 pKa = 7.07ASNPVYY239 pKa = 9.86ATLKK243 pKa = 9.47IRR245 pKa = 11.84IYY247 pKa = 10.66FYY249 pKa = 11.18DD250 pKa = 3.06SVMNN254 pKa = 4.52

Molecular weight: 29.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

969

87

368

193.8

22.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.47 ± 0.868

1.858 ± 0.378

4.85 ± 0.699

5.47 ± 0.863

3.715 ± 0.45

6.089 ± 0.793

4.231 ± 0.496

5.366 ± 0.995

5.057 ± 1.18

6.398 ± 0.961

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.167 ± 0.593

5.573 ± 0.929

5.986 ± 0.837

4.231 ± 0.833

8.05 ± 1.657

7.946 ± 1.739

5.366 ± 1.434

6.089 ± 1.193

1.858 ± 0.1

4.231 ± 1.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski