Streptomyces showdoensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6933 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P2GW93|A0A2P2GW93_9ACTN Glutamate--tRNA ligase OS=Streptomyces showdoensis OX=68268 GN=gltX PE=3 SV=1
MM1 pKa = 7.15ITTDD5 pKa = 4.28FSPGSPCWLDD15 pKa = 3.92LGAPDD20 pKa = 4.22VPAAAAFYY28 pKa = 10.05GAVFGWEE35 pKa = 4.16YY36 pKa = 11.23EE37 pKa = 4.32SMDD40 pKa = 3.55GDD42 pKa = 3.82EE43 pKa = 4.96GGGGAEE49 pKa = 4.29GTDD52 pKa = 3.46AGSGADD58 pKa = 3.62SGAEE62 pKa = 3.89NTGTTEE68 pKa = 3.95DD69 pKa = 4.11FEE71 pKa = 6.91GGMFRR76 pKa = 11.84KK77 pKa = 10.06DD78 pKa = 3.17GRR80 pKa = 11.84IVAGLGSLTEE90 pKa = 4.07EE91 pKa = 4.92GARR94 pKa = 11.84SAWMIYY100 pKa = 10.45YY101 pKa = 10.44SVDD104 pKa = 3.33DD105 pKa = 4.6ADD107 pKa = 3.85ATTEE111 pKa = 3.92AVVQGGGTVRR121 pKa = 11.84VPPRR125 pKa = 11.84DD126 pKa = 3.29LDD128 pKa = 3.26EE129 pKa = 4.5WGRR132 pKa = 11.84MAQYY136 pKa = 10.81SDD138 pKa = 3.64PLGGQFAVWQPGSNKK153 pKa = 9.73GAEE156 pKa = 4.32LVDD159 pKa = 4.07EE160 pKa = 5.15PGSLSWTEE168 pKa = 5.02LYY170 pKa = 10.58TSDD173 pKa = 3.72AAAARR178 pKa = 11.84EE179 pKa = 4.55FYY181 pKa = 11.11ASVLGWWYY189 pKa = 11.39SDD191 pKa = 2.97MEE193 pKa = 4.37MPGGEE198 pKa = 4.25GTYY201 pKa = 10.11TLITPSGLPAEE212 pKa = 4.44RR213 pKa = 11.84MHH215 pKa = 7.16GGLLEE220 pKa = 4.69LSPDD224 pKa = 3.9DD225 pKa = 4.0LALAGGRR232 pKa = 11.84PYY234 pKa = 9.51WHH236 pKa = 7.23PVFAVTDD243 pKa = 4.04CDD245 pKa = 3.29ATAAAVTANGGSVQMGPADD264 pKa = 3.53VPGVGRR270 pKa = 11.84LAVCLDD276 pKa = 3.61PANADD281 pKa = 3.71FVVLAPEE288 pKa = 4.28ANGG291 pKa = 3.42

Molecular weight: 30.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P2GK66|A0A2P2GK66_9ACTN Alpha/beta hydrolase OS=Streptomyces showdoensis OX=68268 GN=VO63_20915 PE=4 SV=1
MM1 pKa = 6.5VAAVAVATVAVTSVRR16 pKa = 11.84RR17 pKa = 11.84PRR19 pKa = 11.84TSVTTAGTTTVAVAVATVAATTVPPSAVTTTAVVAAAAVSVGMTTVAAAAVAGSVATTGVTTTVVAAASAVTTVRR94 pKa = 11.84RR95 pKa = 11.84PRR97 pKa = 11.84TSVTTAGTTTAVAAVATVAATTVPPSVATTTAVVAAVAVATVAVTSVRR145 pKa = 11.84RR146 pKa = 11.84PRR148 pKa = 11.84TSVTTAGTTTVAVAVATVAATTVPPSAVTTTAVVAAVATVAVTSVRR194 pKa = 11.84RR195 pKa = 11.84PRR197 pKa = 11.84TSVTTAGTTTVVAAGSVATTGVTTTGAAAASAVTTAGTTTVVAASVATTVRR248 pKa = 11.84LSVATSAVTSVRR260 pKa = 11.84PPRR263 pKa = 11.84TSVTTAATSAPPRR276 pKa = 11.84TSVTTAATSAPPRR289 pKa = 11.84TSVTTAATTTAVAASVATTGVTSGPVVRR317 pKa = 11.84VVTTTGVVAAVSVAATTVGTTGAVSAAGTTVAGTGAGTTGTVRR360 pKa = 11.84TARR363 pKa = 11.84RR364 pKa = 11.84SSGCRR369 pKa = 11.84SRR371 pKa = 11.84RR372 pKa = 11.84RR373 pKa = 11.84SPVRR377 pKa = 11.84RR378 pKa = 11.84STRR381 pKa = 11.84MCARR385 pKa = 11.84SS386 pKa = 3.1

Molecular weight: 36.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6933

0

6933

2226963

29

3201

321.2

34.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.1 ± 0.047

0.761 ± 0.009

5.823 ± 0.024

5.795 ± 0.035

2.738 ± 0.017

9.733 ± 0.029

2.198 ± 0.012

2.991 ± 0.02

2.145 ± 0.023

10.462 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.679 ± 0.011

1.703 ± 0.016

6.219 ± 0.026

2.564 ± 0.02

8.035 ± 0.032

4.798 ± 0.02

6.132 ± 0.029

8.487 ± 0.03

1.527 ± 0.012

2.109 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski