Pacific flying fox faeces associated circular DNA virus-4

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140CTT3|A0A140CTT3_9VIRU ATP-dependent helicase Rep OS=Pacific flying fox faeces associated circular DNA virus-4 OX=1796013 PE=3 SV=1
MM1 pKa = 7.18QMTHH5 pKa = 7.13IYY7 pKa = 10.04DD8 pKa = 3.38AEE10 pKa = 3.84IRR12 pKa = 11.84LRR14 pKa = 11.84GDD16 pKa = 3.45GTSDD20 pKa = 3.16AGPTHH25 pKa = 6.79FFFYY29 pKa = 10.52ANSMYY34 pKa = 10.63KK35 pKa = 9.93PWAGVNTNSAEE46 pKa = 4.16GGLSIDD52 pKa = 3.4RR53 pKa = 11.84QPRR56 pKa = 11.84YY57 pKa = 9.29FDD59 pKa = 3.36QMEE62 pKa = 4.75KK63 pKa = 9.96IYY65 pKa = 10.44NHH67 pKa = 6.38HH68 pKa = 5.88VVIGSKK74 pKa = 7.91MTVKK78 pKa = 10.29FSAAGLDD85 pKa = 3.6TAAPTRR91 pKa = 11.84LALRR95 pKa = 11.84QTDD98 pKa = 3.73TNIWDD103 pKa = 3.93SATSNPQMFAEE114 pKa = 4.85FGDD117 pKa = 4.03GKK119 pKa = 10.74DD120 pKa = 3.82RR121 pKa = 11.84ILMATANVPVVMTSKK136 pKa = 10.79YY137 pKa = 8.98SAKK140 pKa = 10.13KK141 pKa = 10.23RR142 pKa = 11.84FGKK145 pKa = 10.75GILANSEE152 pKa = 4.58LKK154 pKa = 10.99CSDD157 pKa = 3.88NGNCSEE163 pKa = 3.63GWYY166 pKa = 10.77YY167 pKa = 11.7NMIVQNMNPNAPNDD181 pKa = 2.79VWAIVRR187 pKa = 11.84IEE189 pKa = 4.33YY190 pKa = 7.9ITVWFEE196 pKa = 4.08IKK198 pKa = 8.83PTNQSTIEE206 pKa = 4.04TT207 pKa = 4.09

Molecular weight: 23.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140CTT3|A0A140CTT3_9VIRU ATP-dependent helicase Rep OS=Pacific flying fox faeces associated circular DNA virus-4 OX=1796013 PE=3 SV=1
MM1 pKa = 7.74GSQLTQRR8 pKa = 11.84SRR10 pKa = 11.84NFCFTLNNYY19 pKa = 7.83TEE21 pKa = 4.62SDD23 pKa = 3.73TEE25 pKa = 3.95AVKK28 pKa = 10.84AIDD31 pKa = 3.86CKK33 pKa = 10.67YY34 pKa = 8.5MVVGFEE40 pKa = 4.19VGEE43 pKa = 4.34KK44 pKa = 9.04GTPHH48 pKa = 5.6HH49 pKa = 6.08QGYY52 pKa = 9.9IRR54 pKa = 11.84FEE56 pKa = 4.05NARR59 pKa = 11.84SLGATIKK66 pKa = 10.14QLPGKK71 pKa = 8.98PHH73 pKa = 6.8VEE75 pKa = 4.03VAKK78 pKa = 11.04GNASQNIAYY87 pKa = 7.25CTKK90 pKa = 10.37SGVFFEE96 pKa = 4.73KK97 pKa = 9.62GTRR100 pKa = 11.84PIDD103 pKa = 3.67SEE105 pKa = 4.57TCGRR109 pKa = 11.84NEE111 pKa = 3.57KK112 pKa = 10.46RR113 pKa = 11.84RR114 pKa = 11.84WTDD117 pKa = 2.42ARR119 pKa = 11.84AAAKK123 pKa = 9.98EE124 pKa = 4.02GRR126 pKa = 11.84FDD128 pKa = 6.69DD129 pKa = 4.42IPDD132 pKa = 5.01DD133 pKa = 3.2IYY135 pKa = 10.76MRR137 pKa = 11.84HH138 pKa = 3.91VHH140 pKa = 6.52AIKK143 pKa = 10.31RR144 pKa = 11.84IRR146 pKa = 11.84MEE148 pKa = 5.27DD149 pKa = 3.46GPKK152 pKa = 9.09PTDD155 pKa = 3.4LEE157 pKa = 4.2PRR159 pKa = 11.84DD160 pKa = 4.08TYY162 pKa = 11.35GLWIYY167 pKa = 9.66GPPGTGKK174 pKa = 9.65SHH176 pKa = 6.67FVRR179 pKa = 11.84TNFRR183 pKa = 11.84DD184 pKa = 3.74HH185 pKa = 6.8YY186 pKa = 11.0IKK188 pKa = 10.68GANKK192 pKa = 8.73WWTGYY197 pKa = 10.12CGQKK201 pKa = 10.41YY202 pKa = 7.87VVIDD206 pKa = 3.96EE207 pKa = 5.05LSPASGPEE215 pKa = 3.44LSQYY219 pKa = 7.38MKK221 pKa = 10.45KK222 pKa = 9.26WADD225 pKa = 3.0RR226 pKa = 11.84WSFEE230 pKa = 4.43AEE232 pKa = 4.14TKK234 pKa = 10.45GGNSIIRR241 pKa = 11.84PYY243 pKa = 9.94MIIVTSNYY251 pKa = 9.8SIEE254 pKa = 4.27EE255 pKa = 3.93VFSRR259 pKa = 11.84VDD261 pKa = 2.94AQAIKK266 pKa = 10.65RR267 pKa = 11.84RR268 pKa = 11.84FTEE271 pKa = 3.99IKK273 pKa = 10.04FQQ275 pKa = 3.8

Molecular weight: 31.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

482

207

275

241.0

27.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.469 ± 0.783

1.452 ± 0.31

5.602 ± 0.125

5.809 ± 0.932

4.564 ± 0.138

7.676 ± 0.583

2.282 ± 0.223

6.846 ± 0.053

6.639 ± 0.845

3.32 ± 0.348

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.32 ± 0.964

5.602 ± 1.358

4.564 ± 0.138

3.32 ± 0.04

6.224 ± 1.197

6.224 ± 0.344

7.054 ± 0.431

5.187 ± 0.081

2.282 ± 0.085

4.564 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski