Marinobacter confluentis
Average proteome isoelectric point is 5.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3435 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Z1C6B1|A0A4Z1C6B1_9ALTE SDR family oxidoreductase OS=Marinobacter confluentis OX=1697557 GN=E5Q11_00765 PE=3 SV=1
MM1 pKa = 7.63 KK2 pKa = 10.5 KK3 pKa = 10.41 LLLAVAMSSVAAGSVNAATIYY24 pKa = 10.75 EE25 pKa = 4.31 KK26 pKa = 11.0 DD27 pKa = 3.14 GLTYY31 pKa = 11.0 KK32 pKa = 10.88 LNGDD36 pKa = 3.67 FQVQLRR42 pKa = 11.84 QNIGDD47 pKa = 4.37 DD48 pKa = 3.29 EE49 pKa = 4.59 DD50 pKa = 6.31 LYY52 pKa = 11.88 VDD54 pKa = 4.56 YY55 pKa = 11.38 DD56 pKa = 3.92 DD57 pKa = 6.63 LEE59 pKa = 4.48 LKK61 pKa = 10.81 NYY63 pKa = 9.93 ISYY66 pKa = 11.26 DD67 pKa = 3.07 LGNNMKK73 pKa = 10.73 AFGRR77 pKa = 11.84 VDD79 pKa = 5.01 LDD81 pKa = 4.47 FKK83 pKa = 11.54 DD84 pKa = 3.61 HH85 pKa = 6.88 ANDD88 pKa = 3.88 GGTEE92 pKa = 3.96 EE93 pKa = 4.53 PMEE96 pKa = 4.11 EE97 pKa = 4.53 AYY99 pKa = 10.96 VGLQYY104 pKa = 11.25 GAVSGSLGKK113 pKa = 10.46 QNFASDD119 pKa = 3.3 EE120 pKa = 4.28 FGIEE124 pKa = 3.87 EE125 pKa = 4.58 AYY127 pKa = 7.74 EE128 pKa = 4.08 TPLAEE133 pKa = 5.71 DD134 pKa = 4.52 RR135 pKa = 11.84 YY136 pKa = 11.57 DD137 pKa = 5.81 DD138 pKa = 4.16 IATDD142 pKa = 3.8 GDD144 pKa = 3.58 DD145 pKa = 3.85 TIRR148 pKa = 11.84 FDD150 pKa = 3.55 VDD152 pKa = 3.61 LDD154 pKa = 3.8 TVYY157 pKa = 11.12 LVLSHH162 pKa = 6.54 EE163 pKa = 4.61 LEE165 pKa = 4.7 AEE167 pKa = 4.12 NKK169 pKa = 9.71 QGITGEE175 pKa = 4.2 YY176 pKa = 8.71 TDD178 pKa = 5.47 LFAATEE184 pKa = 4.11 IAGLALAAAYY194 pKa = 7.56 QTYY197 pKa = 8.45 TPLAQDD203 pKa = 3.31 SLDD206 pKa = 3.76 TYY208 pKa = 10.97 GVSASYY214 pKa = 11.34 DD215 pKa = 3.37 FGIASLGADD224 pKa = 3.65 YY225 pKa = 11.29 SSTDD229 pKa = 3.36 DD230 pKa = 3.53 TNTDD234 pKa = 3.42 VEE236 pKa = 4.3 ISLLNVAATFDD247 pKa = 3.58 ITNTTGVALGMQNQEE262 pKa = 4.09 EE263 pKa = 4.79 DD264 pKa = 3.72 NVDD267 pKa = 5.88 DD268 pKa = 3.59 ITGWYY273 pKa = 10.48 ANVTYY278 pKa = 10.68 KK279 pKa = 10.52 FPQHH283 pKa = 6.42 KK284 pKa = 9.68 NVSAFAEE291 pKa = 4.38 VADD294 pKa = 4.04 TDD296 pKa = 4.42 EE297 pKa = 5.78 DD298 pKa = 3.94 NTDD301 pKa = 3.43 LGFLAGMRR309 pKa = 11.84 VKK311 pKa = 10.64 FF312 pKa = 3.9
Molecular weight: 34.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.683
IPC2_protein 3.694
IPC_protein 3.732
Toseland 3.503
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.668
Rodwell 3.554
Grimsley 3.401
Solomon 3.719
Lehninger 3.681
Nozaki 3.834
DTASelect 4.088
Thurlkill 3.554
EMBOSS 3.668
Sillero 3.846
Patrickios 1.291
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.769
Protein with the highest isoelectric point:
>tr|A0A4Z1CGW9|A0A4Z1CGW9_9ALTE Uncharacterized protein OS=Marinobacter confluentis OX=1697557 GN=E5Q11_11895 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.34 RR12 pKa = 11.84 KK13 pKa = 9.1 RR14 pKa = 11.84 VHH16 pKa = 6.26 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATANGRR28 pKa = 11.84 KK29 pKa = 9.34 VISRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.41 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3435
0
3435
1138417
23
2508
331.4
36.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.744 ± 0.039
0.9 ± 0.014
6.016 ± 0.037
6.578 ± 0.037
3.863 ± 0.026
7.842 ± 0.039
2.102 ± 0.021
5.276 ± 0.033
3.601 ± 0.033
10.536 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.58 ± 0.02
3.332 ± 0.024
4.689 ± 0.027
4.105 ± 0.03
6.445 ± 0.036
6.168 ± 0.031
5.191 ± 0.025
7.163 ± 0.031
1.346 ± 0.017
2.525 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here