Nocardioides sp. YIM 123512

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4296 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L7F0F4|A0A6L7F0F4_9ACTN Uncharacterized protein OS=Nocardioides sp. YIM 123512 OX=2691959 GN=GRQ65_12095 PE=4 SV=1
MM1 pKa = 7.47SEE3 pKa = 4.11RR4 pKa = 11.84FGRR7 pKa = 11.84LQKK10 pKa = 9.98ATALVPMAVLSAAVTAGLVGGQVPTPVAAPQSDD43 pKa = 3.39TSLPDD48 pKa = 3.27GTSIPAQAIEE58 pKa = 4.58APASVTGDD66 pKa = 3.28TAGTGTGVGDD76 pKa = 4.86LDD78 pKa = 3.85AQQVVATASTSGIPSAALAAYY99 pKa = 9.2QRR101 pKa = 11.84AEE103 pKa = 4.19TVINAADD110 pKa = 4.13PSCNLPWQLVAAIGRR125 pKa = 11.84VEE127 pKa = 4.46SNHH130 pKa = 5.47GRR132 pKa = 11.84ANGNTLSDD140 pKa = 4.59DD141 pKa = 3.84GVATPGIFGVALDD154 pKa = 3.9GTNRR158 pKa = 11.84TQEE161 pKa = 4.12IVDD164 pKa = 3.91TDD166 pKa = 3.18AGQYY170 pKa = 11.18DD171 pKa = 4.94DD172 pKa = 4.4DD173 pKa = 4.13TTYY176 pKa = 11.65DD177 pKa = 3.42RR178 pKa = 11.84AVGPMQFIPSTWAVVGVDD196 pKa = 3.29ADD198 pKa = 3.86GDD200 pKa = 4.35GVRR203 pKa = 11.84NPQDD207 pKa = 3.01IDD209 pKa = 3.59DD210 pKa = 4.73AALGTAVYY218 pKa = 10.01LCSGDD223 pKa = 5.04DD224 pKa = 4.98DD225 pKa = 5.78LGTDD229 pKa = 3.93AGRR232 pKa = 11.84QAAVYY237 pKa = 9.48RR238 pKa = 11.84YY239 pKa = 8.52NHH241 pKa = 5.56SQSYY245 pKa = 9.89VDD247 pKa = 4.67LVLAIMEE254 pKa = 4.91AYY256 pKa = 9.54MDD258 pKa = 4.34GDD260 pKa = 4.26FSSVPNSTVTAGEE273 pKa = 4.53PILPVPGYY281 pKa = 10.08DD282 pKa = 3.65PSPIKK287 pKa = 10.41QPQQYY292 pKa = 10.01QGNQQSQQPTQQPTEE307 pKa = 4.23APSEE311 pKa = 4.21QPTQQQQPTQQPTQQQQPTQQPTQNQNNQNNNQNQNNNQNQPQVPIATPTLPTLPSTQVAPVDD374 pKa = 4.01DD375 pKa = 4.55VLTAAQAITQCTLDD389 pKa = 4.13GVPILNTQAWQACLDD404 pKa = 4.03NYY406 pKa = 10.19TKK408 pKa = 11.1

Molecular weight: 42.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L7EWU7|A0A6L7EWU7_9ACTN Uncharacterized protein OS=Nocardioides sp. YIM 123512 OX=2691959 GN=GRQ65_03045 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.24TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4296

0

4296

1402411

33

2903

326.4

34.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.415 ± 0.07

0.703 ± 0.01

6.477 ± 0.032

5.786 ± 0.044

2.657 ± 0.023

9.36 ± 0.035

2.158 ± 0.021

2.895 ± 0.03

1.713 ± 0.026

10.332 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.648 ± 0.015

1.551 ± 0.023

5.676 ± 0.032

2.791 ± 0.021

7.813 ± 0.048

5.431 ± 0.028

6.292 ± 0.043

9.911 ± 0.046

1.519 ± 0.017

1.871 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski