Erwiniaceae bacterium PD-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; unclassified Erwiniaceae

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4353 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M8UJ62|A0A6M8UJ62_9GAMM Type II/IV secretion system ATPase OS=Erwiniaceae bacterium PD-1 OX=2740817 GN=PMPD1_3530 PE=4 SV=1
MM1 pKa = 7.73AYY3 pKa = 10.03EE4 pKa = 4.21EE5 pKa = 4.33LLEE8 pKa = 4.18EE9 pKa = 4.15QRR11 pKa = 11.84EE12 pKa = 4.19EE13 pKa = 3.64TRR15 pKa = 11.84LIIEE19 pKa = 4.51EE20 pKa = 4.39LLDD23 pKa = 5.06DD24 pKa = 5.45GSDD27 pKa = 3.44PDD29 pKa = 3.61ALYY32 pKa = 9.93TIEE35 pKa = 5.28HH36 pKa = 6.7HH37 pKa = 6.81LSCNNFDD44 pKa = 4.27SLEE47 pKa = 4.09KK48 pKa = 10.57AAVDD52 pKa = 3.66AFKK55 pKa = 10.85LGYY58 pKa = 10.02EE59 pKa = 4.16VTDD62 pKa = 3.91PEE64 pKa = 4.3EE65 pKa = 5.63LEE67 pKa = 5.32LEE69 pKa = 4.74DD70 pKa = 4.51GTTVMCVDD78 pKa = 4.17ILSEE82 pKa = 3.89LALNAEE88 pKa = 4.81LIDD91 pKa = 3.87VQVEE95 pKa = 4.03QLVNMAAKK103 pKa = 10.29YY104 pKa = 10.2HH105 pKa = 5.94VDD107 pKa = 3.22YY108 pKa = 11.07DD109 pKa = 3.35GWGTYY114 pKa = 10.12FEE116 pKa = 5.89DD117 pKa = 4.94PDD119 pKa = 4.22AEE121 pKa = 4.22EE122 pKa = 5.16DD123 pKa = 3.87DD124 pKa = 4.82EE125 pKa = 5.51EE126 pKa = 6.47GGTYY130 pKa = 9.17IDD132 pKa = 4.58EE133 pKa = 5.4DD134 pKa = 4.53DD135 pKa = 4.93DD136 pKa = 4.58GVRR139 pKa = 11.84HH140 pKa = 6.05

Molecular weight: 15.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M8U352|A0A6M8U352_9GAMM ATP-grasp domain-containing protein OS=Erwiniaceae bacterium PD-1 OX=2740817 GN=PMPD1_0025 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LTVSKK46 pKa = 10.99

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4353

0

4353

1371518

44

3511

315.1

34.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.133 ± 0.043

0.992 ± 0.012

5.104 ± 0.03

5.501 ± 0.04

3.808 ± 0.027

7.474 ± 0.036

2.329 ± 0.02

5.651 ± 0.031

3.936 ± 0.034

11.049 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.634 ± 0.018

3.673 ± 0.025

4.52 ± 0.026

4.797 ± 0.034

5.922 ± 0.038

5.968 ± 0.031

5.328 ± 0.031

6.928 ± 0.032

1.528 ± 0.017

2.725 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski