Sonchus yellow net virus (SYNV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Nucleorhabdovirus

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P31332|L_SYNV RNA-directed RNA polymerase L OS=Sonchus yellow net virus OX=11307 GN=L PE=2 SV=1
MM1 pKa = 7.07SHH3 pKa = 6.53IMNLLVISFVLAGSSWSLLGYY24 pKa = 10.25QDD26 pKa = 4.9DD27 pKa = 4.41FSSKK31 pKa = 10.19RR32 pKa = 11.84SGALASNPTYY42 pKa = 10.73NLPQDD47 pKa = 3.27KK48 pKa = 10.87GYY50 pKa = 10.71GRR52 pKa = 11.84DD53 pKa = 3.31MYY55 pKa = 10.5QPYY58 pKa = 9.85YY59 pKa = 10.01ICEE62 pKa = 4.26PDD64 pKa = 3.39NDD66 pKa = 4.11GSALTLPSWHH76 pKa = 6.37YY77 pKa = 10.57SCKK80 pKa = 9.35EE81 pKa = 3.76SCMGNHH87 pKa = 7.5LKK89 pKa = 10.45RR90 pKa = 11.84VVNITGARR98 pKa = 11.84WNYY101 pKa = 9.61VGISIPVFKK110 pKa = 10.57IVTNEE115 pKa = 3.44VCYY118 pKa = 10.13TSHH121 pKa = 6.31EE122 pKa = 4.47NVWGYY127 pKa = 10.68CSQYY131 pKa = 10.47QISRR135 pKa = 11.84PVATQKK141 pKa = 11.16SDD143 pKa = 3.37VSCITSSMWDD153 pKa = 3.41NDD155 pKa = 3.07KK156 pKa = 11.61SPIGSLYY163 pKa = 10.87NIVNSNEE170 pKa = 3.81AEE172 pKa = 3.8CDD174 pKa = 3.73YY175 pKa = 11.12FSDD178 pKa = 3.76ITDD181 pKa = 3.63CNRR184 pKa = 11.84DD185 pKa = 3.33YY186 pKa = 11.32QIFKK190 pKa = 10.84RR191 pKa = 11.84EE192 pKa = 3.58GKK194 pKa = 9.96LIKK197 pKa = 10.51RR198 pKa = 11.84SDD200 pKa = 3.86DD201 pKa = 3.64SPLEE205 pKa = 3.93LSIVTDD211 pKa = 5.22GIRR214 pKa = 11.84TDD216 pKa = 3.53PASEE220 pKa = 4.16YY221 pKa = 11.06LSLDD225 pKa = 3.46DD226 pKa = 6.25VSWFWKK232 pKa = 10.42LPNNDD237 pKa = 3.49MSPPCGWEE245 pKa = 3.82KK246 pKa = 9.02TQKK249 pKa = 10.45LSCSYY254 pKa = 11.08TDD256 pKa = 3.14TTDD259 pKa = 3.45VIKK262 pKa = 10.79CNSIGYY268 pKa = 7.22TYY270 pKa = 10.32NIQGISKK277 pKa = 10.5KK278 pKa = 7.87STCAGNIYY286 pKa = 9.28DD287 pKa = 4.27TDD289 pKa = 4.02GPFPFFYY296 pKa = 10.62DD297 pKa = 3.34AEE299 pKa = 4.27EE300 pKa = 5.11ALMSTDD306 pKa = 4.04DD307 pKa = 3.6ACGKK311 pKa = 10.14AKK313 pKa = 10.02QGKK316 pKa = 8.29PDD318 pKa = 3.65ADD320 pKa = 3.12IAFIEE325 pKa = 4.67GVNRR329 pKa = 11.84AFEE332 pKa = 4.64DD333 pKa = 4.64LEE335 pKa = 4.26LTYY338 pKa = 10.97CSATCDD344 pKa = 3.17LFARR348 pKa = 11.84QGTPNEE354 pKa = 4.09DD355 pKa = 3.21HH356 pKa = 7.34VLDD359 pKa = 4.27TPIGTWRR366 pKa = 11.84YY367 pKa = 8.61VMRR370 pKa = 11.84DD371 pKa = 3.4NLDD374 pKa = 3.81PALVPCLPTSNWTISDD390 pKa = 3.67PTTICHH396 pKa = 6.15GKK398 pKa = 9.54DD399 pKa = 3.11HH400 pKa = 6.74ILVVDD405 pKa = 4.28TATGHH410 pKa = 5.81SGSWDD415 pKa = 3.45TKK417 pKa = 9.56KK418 pKa = 10.91DD419 pKa = 3.73YY420 pKa = 10.83IITGEE425 pKa = 4.15VCNTNNDD432 pKa = 3.72EE433 pKa = 4.72MGDD436 pKa = 4.08DD437 pKa = 3.97YY438 pKa = 11.86DD439 pKa = 4.55GMRR442 pKa = 11.84DD443 pKa = 3.3KK444 pKa = 10.72ILRR447 pKa = 11.84GEE449 pKa = 4.18TIEE452 pKa = 4.07IKK454 pKa = 10.32FWTGDD459 pKa = 4.02IIRR462 pKa = 11.84MAPPYY467 pKa = 10.44DD468 pKa = 3.47NPEE471 pKa = 4.17WIKK474 pKa = 11.11GSVLFRR480 pKa = 11.84QNPGWFSSVEE490 pKa = 3.89LNKK493 pKa = 11.05DD494 pKa = 3.62MIHH497 pKa = 5.89TRR499 pKa = 11.84DD500 pKa = 4.11NITDD504 pKa = 4.52LLTVMVQNATAEE516 pKa = 4.21VMYY519 pKa = 10.73KK520 pKa = 10.44RR521 pKa = 11.84LDD523 pKa = 3.78PKK525 pKa = 9.91TMKK528 pKa = 10.29HH529 pKa = 5.54ILFAEE534 pKa = 4.28IVDD537 pKa = 4.07GVGNVSGKK545 pKa = 10.24ISGFLTGLFGGFTKK559 pKa = 10.66AVIIVASLAICYY571 pKa = 9.43IVLSVLWKK579 pKa = 9.37VRR581 pKa = 11.84LVASIFNSAKK591 pKa = 9.73KK592 pKa = 10.11KK593 pKa = 9.69RR594 pKa = 11.84VRR596 pKa = 11.84ISDD599 pKa = 3.47ILDD602 pKa = 3.8EE603 pKa = 4.55EE604 pKa = 4.4PHH606 pKa = 7.09RR607 pKa = 11.84IQQSRR612 pKa = 11.84PTLSRR617 pKa = 11.84KK618 pKa = 9.62KK619 pKa = 9.2KK620 pKa = 7.5TRR622 pKa = 11.84EE623 pKa = 4.0SIQMLLNDD631 pKa = 3.71II632 pKa = 4.81

Molecular weight: 71.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q89914|MVP_SYNV Movement protein OS=Sonchus yellow net virus OX=11307 GN=sc4 PE=1 SV=1
MM1 pKa = 7.67EE2 pKa = 5.27GLSSKK7 pKa = 10.05AQTMGRR13 pKa = 11.84EE14 pKa = 4.04DD15 pKa = 4.24DD16 pKa = 3.82NRR18 pKa = 11.84SSKK21 pKa = 10.44MKK23 pKa = 10.02VFHH26 pKa = 6.86SEE28 pKa = 3.48LVYY31 pKa = 11.11GDD33 pKa = 3.67NHH35 pKa = 6.7NISIKK40 pKa = 10.57KK41 pKa = 10.05ADD43 pKa = 3.66LTGQHH48 pKa = 6.64KK49 pKa = 10.18MMLLLSSALRR59 pKa = 11.84IGSVHH64 pKa = 6.26MDD66 pKa = 3.06VSRR69 pKa = 11.84ILVKK73 pKa = 9.07WCPYY77 pKa = 8.06ITPNMNTTIGITIKK91 pKa = 10.82NNHH94 pKa = 6.81HH95 pKa = 7.44DD96 pKa = 4.15DD97 pKa = 3.51MSNINDD103 pKa = 3.35MSTYY107 pKa = 10.3ISVKK111 pKa = 10.25GKK113 pKa = 9.67MSEE116 pKa = 3.82ALQITWHH123 pKa = 6.4PASTLVYY130 pKa = 9.32KK131 pKa = 10.72KK132 pKa = 10.77GMSCIFPWVVDD143 pKa = 3.42VDD145 pKa = 3.94TGSTEE150 pKa = 4.06QEE152 pKa = 4.18SGSPALGEE160 pKa = 4.0IKK162 pKa = 9.83IWCYY166 pKa = 11.17FKK168 pKa = 10.32MQYY171 pKa = 9.97HH172 pKa = 6.23KK173 pKa = 10.69PSTRR177 pKa = 11.84HH178 pKa = 4.61IARR181 pKa = 11.84AEE183 pKa = 3.79IAPSIEE189 pKa = 3.69WGNTNFPYY197 pKa = 10.35YY198 pKa = 10.84VPFAMIRR205 pKa = 11.84RR206 pKa = 11.84ARR208 pKa = 11.84GIRR211 pKa = 11.84PLDD214 pKa = 3.42VFSTNQYY221 pKa = 11.65SMFLEE226 pKa = 4.45DD227 pKa = 3.7VIKK230 pKa = 10.74HH231 pKa = 5.65VGTDD235 pKa = 3.64SIKK238 pKa = 10.75EE239 pKa = 3.98SDD241 pKa = 3.74IVPIMSTMSQEE252 pKa = 4.03DD253 pKa = 3.39MMMINEE259 pKa = 4.15KK260 pKa = 10.91NKK262 pKa = 8.88TCLLKK267 pKa = 10.78RR268 pKa = 11.84GGSYY272 pKa = 10.41CSCKK276 pKa = 10.46DD277 pKa = 3.37VIEE280 pKa = 4.33NVVKK284 pKa = 10.18EE285 pKa = 4.05INMNRR290 pKa = 11.84DD291 pKa = 2.95RR292 pKa = 11.84KK293 pKa = 9.87YY294 pKa = 11.23DD295 pKa = 3.49NHH297 pKa = 6.56GLLLSGYY304 pKa = 9.27IAGSTSGRR312 pKa = 11.84FQTVPMLSDD321 pKa = 3.18ISYY324 pKa = 11.01

Molecular weight: 36.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

4178

286

2116

696.3

78.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.05 ± 0.695

2.106 ± 0.243

6.726 ± 0.433

5.05 ± 0.354

3.255 ± 0.316

5.505 ± 0.454

2.8 ± 0.308

8.042 ± 0.403

6.462 ± 0.322

8.664 ± 0.991

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.303 ± 0.429

5.218 ± 0.119

3.71 ± 0.346

2.824 ± 0.12

4.859 ± 0.556

9.023 ± 0.253

6.415 ± 0.261

5.385 ± 0.339

1.316 ± 0.24

4.284 ± 0.118
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski