Brucella phage Tb

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Perisivirus; Brucella virus Tb

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H2EI56|H2EI56_9CAUD Uncharacterized protein OS=Brucella phage Tb OX=1133292 GN=Tb_ORF14 PE=4 SV=1
MM1 pKa = 7.44ANSVPFRR8 pKa = 11.84TTPQLGPQLNDD19 pKa = 3.04VFVGLPYY26 pKa = 9.92WDD28 pKa = 3.43LTGIQGAGGATVSGITEE45 pKa = 4.16PSYY48 pKa = 11.53KK49 pKa = 10.29LGNRR53 pKa = 11.84EE54 pKa = 4.15TGDD57 pKa = 3.9DD58 pKa = 4.2GYY60 pKa = 11.51DD61 pKa = 3.75YY62 pKa = 11.12IWVQNGTTALTASARR77 pKa = 11.84VNMDD81 pKa = 2.74SDD83 pKa = 4.19FVTTANASGVLQAPDD98 pKa = 3.29VAVPAGAYY106 pKa = 7.73FHH108 pKa = 6.83ARR110 pKa = 11.84VYY112 pKa = 11.28ALL114 pKa = 3.9

Molecular weight: 12.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H2EI62|H2EI62_9CAUD Putative peptidoglycan hydrolase OS=Brucella phage Tb OX=1133292 GN=Tb_ORF20 PE=4 SV=1
MM1 pKa = 7.6LFNFGFRR8 pKa = 11.84PQGQMPFMQNQQINTFAANNLSGYY32 pKa = 10.14NNLGNRR38 pKa = 11.84MSNNPNAYY46 pKa = 8.53QQIPMAPASGALIDD60 pKa = 4.45SGLVNNQSFMNNISPFASANPSSAMMANMGFNQGNQYY97 pKa = 8.54TLTPQQQQTPIAQNMMQSNIPDD119 pKa = 3.48VKK121 pKa = 10.23KK122 pKa = 10.41QAIGSIMSGGLRR134 pKa = 11.84GGNRR138 pKa = 3.24

Molecular weight: 14.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

13057

30

848

225.1

24.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.504 ± 0.529

1.118 ± 0.187

5.905 ± 0.199

6.472 ± 0.583

3.316 ± 0.183

8.21 ± 0.589

1.616 ± 0.195

5.698 ± 0.259

5.338 ± 0.487

7.237 ± 0.211

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.665 ± 0.174

4.718 ± 0.303

4.695 ± 0.281

4.381 ± 0.482

5.43 ± 0.294

6.954 ± 0.277

5.606 ± 0.329

6.456 ± 0.292

1.555 ± 0.14

3.125 ± 0.204
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski