Amino acid dipepetide frequency for Pseudomonas phage Pf-10

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.636AlaAla: 10.636 ± 1.085
0.495AlaCys: 0.495 ± 0.192
7.173AlaAsp: 7.173 ± 1.079
6.019AlaGlu: 6.019 ± 0.762
3.463AlaPhe: 3.463 ± 0.476
8.492AlaGly: 8.492 ± 0.914
2.309AlaHis: 2.309 ± 0.537
4.37AlaIle: 4.37 ± 0.615
6.596AlaLys: 6.596 ± 0.672
8.739AlaLeu: 8.739 ± 0.934
3.463AlaMet: 3.463 ± 0.602
2.803AlaAsn: 2.803 ± 0.401
3.215AlaPro: 3.215 ± 0.565
5.112AlaGln: 5.112 ± 0.901
6.678AlaArg: 6.678 ± 0.679
6.019AlaSer: 6.019 ± 1.004
5.689AlaThr: 5.689 ± 1.27
6.596AlaVal: 6.596 ± 0.645
1.319AlaTrp: 1.319 ± 0.344
2.886AlaTyr: 2.886 ± 0.505
0.0AlaXaa: 0.0 ± 0.0
Cys
1.072CysAla: 1.072 ± 0.29
0.0CysCys: 0.0 ± 0.0
0.742CysAsp: 0.742 ± 0.262
0.412CysGlu: 0.412 ± 0.185
0.412CysPhe: 0.412 ± 0.192
0.412CysGly: 0.412 ± 0.189
0.247CysHis: 0.247 ± 0.148
0.33CysIle: 0.33 ± 0.162
0.824CysLys: 0.824 ± 0.301
0.989CysLeu: 0.989 ± 0.331
0.247CysMet: 0.247 ± 0.149
0.165CysAsn: 0.165 ± 0.117
0.247CysPro: 0.247 ± 0.129
0.247CysGln: 0.247 ± 0.135
0.495CysArg: 0.495 ± 0.211
0.247CysSer: 0.247 ± 0.135
0.082CysThr: 0.082 ± 0.079
0.33CysVal: 0.33 ± 0.144
0.082CysTrp: 0.082 ± 0.083
0.33CysTyr: 0.33 ± 0.179
0.0CysXaa: 0.0 ± 0.0
Asp
8.327AspAla: 8.327 ± 0.966
0.412AspCys: 0.412 ± 0.249
3.875AspAsp: 3.875 ± 0.638
3.875AspGlu: 3.875 ± 0.565
2.886AspPhe: 2.886 ± 0.422
6.761AspGly: 6.761 ± 0.907
1.319AspHis: 1.319 ± 0.314
3.875AspIle: 3.875 ± 0.481
2.721AspLys: 2.721 ± 0.472
4.782AspLeu: 4.782 ± 0.595
1.649AspMet: 1.649 ± 0.404
2.061AspAsn: 2.061 ± 0.216
2.803AspPro: 2.803 ± 0.554
2.968AspGln: 2.968 ± 0.516
3.793AspArg: 3.793 ± 0.671
3.051AspSer: 3.051 ± 0.637
2.968AspThr: 2.968 ± 0.548
3.051AspVal: 3.051 ± 0.517
1.072AspTrp: 1.072 ± 0.252
1.731AspTyr: 1.731 ± 0.406
0.0AspXaa: 0.0 ± 0.0
Glu
8.904GluAla: 8.904 ± 0.813
0.989GluCys: 0.989 ± 0.339
3.957GluAsp: 3.957 ± 0.532
5.606GluGlu: 5.606 ± 0.763
2.721GluPhe: 2.721 ± 0.405
5.277GluGly: 5.277 ± 0.726
1.649GluHis: 1.649 ± 0.419
3.298GluIle: 3.298 ± 0.436
2.968GluLys: 2.968 ± 0.513
6.101GluLeu: 6.101 ± 0.827
1.484GluMet: 1.484 ± 0.315
2.721GluAsn: 2.721 ± 0.422
2.391GluPro: 2.391 ± 0.531
2.721GluGln: 2.721 ± 0.61
4.205GluArg: 4.205 ± 0.431
3.133GluSer: 3.133 ± 0.577
4.205GluThr: 4.205 ± 0.53
4.122GluVal: 4.122 ± 0.587
1.402GluTrp: 1.402 ± 0.401
2.061GluTyr: 2.061 ± 0.38
0.0GluXaa: 0.0 ± 0.0
Phe
2.226PheAla: 2.226 ± 0.41
0.33PheCys: 0.33 ± 0.211
2.721PheAsp: 2.721 ± 0.475
2.309PheGlu: 2.309 ± 0.374
1.566PhePhe: 1.566 ± 0.363
2.556PheGly: 2.556 ± 0.377
0.824PheHis: 0.824 ± 0.213
1.484PheIle: 1.484 ± 0.321
1.731PheLys: 1.731 ± 0.394
4.04PheLeu: 4.04 ± 0.539
1.484PheMet: 1.484 ± 0.27
1.814PheAsn: 1.814 ± 0.392
1.402PhePro: 1.402 ± 0.358
1.319PheGln: 1.319 ± 0.281
2.309PheArg: 2.309 ± 0.353
2.061PheSer: 2.061 ± 0.364
2.226PheThr: 2.226 ± 0.303
2.638PheVal: 2.638 ± 0.444
0.412PheTrp: 0.412 ± 0.17
0.66PheTyr: 0.66 ± 0.278
0.0PheXaa: 0.0 ± 0.0
Gly
8.245GlyAla: 8.245 ± 1.132
0.66GlyCys: 0.66 ± 0.207
4.947GlyAsp: 4.947 ± 0.621
5.277GlyGlu: 5.277 ± 0.824
3.215GlyPhe: 3.215 ± 0.549
5.854GlyGly: 5.854 ± 0.746
2.226GlyHis: 2.226 ± 0.423
4.535GlyIle: 4.535 ± 0.773
5.194GlyLys: 5.194 ± 0.872
6.596GlyLeu: 6.596 ± 0.646
1.731GlyMet: 1.731 ± 0.347
3.051GlyAsn: 3.051 ± 0.57
2.061GlyPro: 2.061 ± 0.378
3.133GlyGln: 3.133 ± 0.414
5.277GlyArg: 5.277 ± 0.778
5.606GlySer: 5.606 ± 0.865
4.864GlyThr: 4.864 ± 0.593
4.947GlyVal: 4.947 ± 0.764
1.237GlyTrp: 1.237 ± 0.229
2.968GlyTyr: 2.968 ± 0.554
0.0GlyXaa: 0.0 ± 0.0
His
1.896HisAla: 1.896 ± 0.582
0.412HisCys: 0.412 ± 0.204
0.989HisAsp: 0.989 ± 0.312
1.896HisGlu: 1.896 ± 0.405
0.824HisPhe: 0.824 ± 0.239
1.896HisGly: 1.896 ± 0.386
0.33HisHis: 0.33 ± 0.175
1.154HisIle: 1.154 ± 0.306
1.402HisLys: 1.402 ± 0.314
2.144HisLeu: 2.144 ± 0.319
0.824HisMet: 0.824 ± 0.208
0.989HisAsn: 0.989 ± 0.222
0.577HisPro: 0.577 ± 0.239
0.577HisGln: 0.577 ± 0.189
1.484HisArg: 1.484 ± 0.429
0.989HisSer: 0.989 ± 0.27
1.402HisThr: 1.402 ± 0.41
1.154HisVal: 1.154 ± 0.291
0.495HisTrp: 0.495 ± 0.181
0.907HisTyr: 0.907 ± 0.275
0.0HisXaa: 0.0 ± 0.0
Ile
2.886IleAla: 2.886 ± 0.56
0.742IleCys: 0.742 ± 0.217
3.545IleAsp: 3.545 ± 0.701
2.886IleGlu: 2.886 ± 0.466
0.989IlePhe: 0.989 ± 0.3
3.545IleGly: 3.545 ± 0.463
0.742IleHis: 0.742 ± 0.272
1.896IleIle: 1.896 ± 0.336
3.71IleLys: 3.71 ± 0.72
3.793IleLeu: 3.793 ± 0.507
1.566IleMet: 1.566 ± 0.311
1.484IleAsn: 1.484 ± 0.35
2.556IlePro: 2.556 ± 0.508
2.391IleGln: 2.391 ± 0.381
3.298IleArg: 3.298 ± 0.379
1.979IleSer: 1.979 ± 0.339
3.133IleThr: 3.133 ± 0.498
3.463IleVal: 3.463 ± 0.598
0.412IleTrp: 0.412 ± 0.157
1.319IleTyr: 1.319 ± 0.358
0.0IleXaa: 0.0 ± 0.0
Lys
7.255LysAla: 7.255 ± 0.902
0.412LysCys: 0.412 ± 0.199
4.37LysAsp: 4.37 ± 0.634
3.545LysGlu: 3.545 ± 0.608
2.144LysPhe: 2.144 ± 0.305
5.689LysGly: 5.689 ± 0.609
1.814LysHis: 1.814 ± 0.5
2.391LysIle: 2.391 ± 0.299
2.968LysLys: 2.968 ± 0.745
4.699LysLeu: 4.699 ± 0.609
2.309LysMet: 2.309 ± 0.458
1.731LysAsn: 1.731 ± 0.358
2.968LysPro: 2.968 ± 0.466
1.979LysGln: 1.979 ± 0.472
3.38LysArg: 3.38 ± 0.757
2.391LysSer: 2.391 ± 0.486
2.886LysThr: 2.886 ± 0.472
5.112LysVal: 5.112 ± 0.778
0.412LysTrp: 0.412 ± 0.171
1.896LysTyr: 1.896 ± 0.376
0.0LysXaa: 0.0 ± 0.0
Leu
8.327LeuAla: 8.327 ± 0.991
0.247LeuCys: 0.247 ± 0.17
4.947LeuAsp: 4.947 ± 0.693
6.926LeuGlu: 6.926 ± 0.889
2.638LeuPhe: 2.638 ± 0.508
5.194LeuGly: 5.194 ± 0.661
2.061LeuHis: 2.061 ± 0.318
4.287LeuIle: 4.287 ± 0.501
6.101LeuLys: 6.101 ± 0.776
5.359LeuLeu: 5.359 ± 0.666
2.556LeuMet: 2.556 ± 0.415
4.287LeuAsn: 4.287 ± 0.556
3.133LeuPro: 3.133 ± 0.397
3.628LeuGln: 3.628 ± 0.613
4.452LeuArg: 4.452 ± 0.501
3.71LeuSer: 3.71 ± 0.525
4.535LeuThr: 4.535 ± 0.567
6.596LeuVal: 6.596 ± 0.608
0.907LeuTrp: 0.907 ± 0.284
2.226LeuTyr: 2.226 ± 0.436
0.0LeuXaa: 0.0 ± 0.0
Met
3.875MetAla: 3.875 ± 0.599
0.247MetCys: 0.247 ± 0.175
1.566MetAsp: 1.566 ± 0.318
2.473MetGlu: 2.473 ± 0.427
0.824MetPhe: 0.824 ± 0.211
2.638MetGly: 2.638 ± 0.438
0.495MetHis: 0.495 ± 0.219
1.566MetIle: 1.566 ± 0.305
1.649MetLys: 1.649 ± 0.417
2.061MetLeu: 2.061 ± 0.412
0.577MetMet: 0.577 ± 0.2
0.495MetAsn: 0.495 ± 0.18
1.402MetPro: 1.402 ± 0.24
1.237MetGln: 1.237 ± 0.27
0.989MetArg: 0.989 ± 0.262
2.226MetSer: 2.226 ± 0.379
2.968MetThr: 2.968 ± 0.561
1.814MetVal: 1.814 ± 0.279
0.495MetTrp: 0.495 ± 0.182
0.33MetTyr: 0.33 ± 0.141
0.0MetXaa: 0.0 ± 0.0
Asn
3.463AsnAla: 3.463 ± 0.566
0.247AsnCys: 0.247 ± 0.132
2.226AsnAsp: 2.226 ± 0.381
2.391AsnGlu: 2.391 ± 0.42
1.896AsnPhe: 1.896 ± 0.375
3.38AsnGly: 3.38 ± 0.491
0.989AsnHis: 0.989 ± 0.336
1.649AsnIle: 1.649 ± 0.401
1.814AsnLys: 1.814 ± 0.352
3.463AsnLeu: 3.463 ± 0.379
1.237AsnMet: 1.237 ± 0.386
0.989AsnAsn: 0.989 ± 0.34
3.051AsnPro: 3.051 ± 0.503
1.237AsnGln: 1.237 ± 0.232
2.556AsnArg: 2.556 ± 0.572
1.484AsnSer: 1.484 ± 0.349
1.731AsnThr: 1.731 ± 0.375
2.803AsnVal: 2.803 ± 0.469
0.412AsnTrp: 0.412 ± 0.153
1.154AsnTyr: 1.154 ± 0.38
0.0AsnXaa: 0.0 ± 0.0
Pro
2.309ProAla: 2.309 ± 0.36
0.247ProCys: 0.247 ± 0.146
3.38ProAsp: 3.38 ± 0.586
3.628ProGlu: 3.628 ± 0.816
1.402ProPhe: 1.402 ± 0.261
3.38ProGly: 3.38 ± 0.614
1.072ProHis: 1.072 ± 0.272
1.154ProIle: 1.154 ± 0.3
2.226ProLys: 2.226 ± 0.472
2.886ProLeu: 2.886 ± 0.511
0.742ProMet: 0.742 ± 0.201
2.061ProAsn: 2.061 ± 0.255
1.237ProPro: 1.237 ± 0.382
1.649ProGln: 1.649 ± 0.374
2.226ProArg: 2.226 ± 0.353
1.814ProSer: 1.814 ± 0.308
2.144ProThr: 2.144 ± 0.458
3.298ProVal: 3.298 ± 0.649
0.495ProTrp: 0.495 ± 0.207
0.989ProTyr: 0.989 ± 0.316
0.0ProXaa: 0.0 ± 0.0
Gln
6.101GlnAla: 6.101 ± 1.022
0.247GlnCys: 0.247 ± 0.133
2.721GlnAsp: 2.721 ± 0.617
1.896GlnGlu: 1.896 ± 0.35
1.896GlnPhe: 1.896 ± 0.357
3.875GlnGly: 3.875 ± 0.473
0.742GlnHis: 0.742 ± 0.287
1.896GlnIle: 1.896 ± 0.349
2.309GlnLys: 2.309 ± 0.415
3.793GlnLeu: 3.793 ± 0.471
1.319GlnMet: 1.319 ± 0.339
1.402GlnAsn: 1.402 ± 0.405
0.742GlnPro: 0.742 ± 0.257
2.309GlnGln: 2.309 ± 0.37
2.721GlnArg: 2.721 ± 0.738
2.391GlnSer: 2.391 ± 0.615
1.731GlnThr: 1.731 ± 0.413
2.556GlnVal: 2.556 ± 0.548
0.66GlnTrp: 0.66 ± 0.224
1.237GlnTyr: 1.237 ± 0.37
0.0GlnXaa: 0.0 ± 0.0
Arg
5.029ArgAla: 5.029 ± 0.552
0.33ArgCys: 0.33 ± 0.165
3.957ArgAsp: 3.957 ± 0.676
4.617ArgGlu: 4.617 ± 0.706
2.061ArgPhe: 2.061 ± 0.438
4.699ArgGly: 4.699 ± 0.561
1.154ArgHis: 1.154 ± 0.288
2.473ArgIle: 2.473 ± 0.434
3.463ArgLys: 3.463 ± 0.568
4.864ArgLeu: 4.864 ± 0.685
1.484ArgMet: 1.484 ± 0.263
3.051ArgAsn: 3.051 ± 0.529
1.896ArgPro: 1.896 ± 0.295
2.968ArgGln: 2.968 ± 0.573
3.133ArgArg: 3.133 ± 0.369
3.71ArgSer: 3.71 ± 0.596
2.556ArgThr: 2.556 ± 0.476
4.122ArgVal: 4.122 ± 0.584
0.824ArgTrp: 0.824 ± 0.244
1.896ArgTyr: 1.896 ± 0.304
0.0ArgXaa: 0.0 ± 0.0
Ser
5.277SerAla: 5.277 ± 0.96
0.412SerCys: 0.412 ± 0.178
3.875SerAsp: 3.875 ± 0.505
3.875SerGlu: 3.875 ± 0.593
2.803SerPhe: 2.803 ± 0.515
4.699SerGly: 4.699 ± 0.612
1.154SerHis: 1.154 ± 0.287
1.896SerIle: 1.896 ± 0.302
3.793SerLys: 3.793 ± 0.656
4.37SerLeu: 4.37 ± 0.537
1.979SerMet: 1.979 ± 0.546
1.814SerAsn: 1.814 ± 0.428
1.979SerPro: 1.979 ± 0.387
1.484SerGln: 1.484 ± 0.374
2.226SerArg: 2.226 ± 0.471
3.133SerSer: 3.133 ± 0.545
2.144SerThr: 2.144 ± 0.409
3.957SerVal: 3.957 ± 0.588
0.742SerTrp: 0.742 ± 0.182
1.649SerTyr: 1.649 ± 0.427
0.0SerXaa: 0.0 ± 0.0
Thr
4.535ThrAla: 4.535 ± 0.63
0.33ThrCys: 0.33 ± 0.185
3.298ThrAsp: 3.298 ± 0.527
4.04ThrGlu: 4.04 ± 0.494
1.731ThrPhe: 1.731 ± 0.482
4.947ThrGly: 4.947 ± 0.677
1.072ThrHis: 1.072 ± 0.293
3.38ThrIle: 3.38 ± 0.558
4.205ThrLys: 4.205 ± 0.46
4.782ThrLeu: 4.782 ± 0.788
1.566ThrMet: 1.566 ± 0.395
2.061ThrAsn: 2.061 ± 0.392
2.638ThrPro: 2.638 ± 0.446
2.144ThrGln: 2.144 ± 0.552
2.309ThrArg: 2.309 ± 0.532
3.051ThrSer: 3.051 ± 0.542
4.287ThrThr: 4.287 ± 0.75
4.04ThrVal: 4.04 ± 0.793
0.495ThrTrp: 0.495 ± 0.198
1.731ThrTyr: 1.731 ± 0.373
0.0ThrXaa: 0.0 ± 0.0
Val
7.338ValAla: 7.338 ± 0.77
0.577ValCys: 0.577 ± 0.26
3.133ValAsp: 3.133 ± 0.435
5.854ValGlu: 5.854 ± 0.641
1.566ValPhe: 1.566 ± 0.308
4.782ValGly: 4.782 ± 0.605
1.237ValHis: 1.237 ± 0.269
3.215ValIle: 3.215 ± 0.559
3.298ValLys: 3.298 ± 0.527
5.029ValLeu: 5.029 ± 0.582
1.814ValMet: 1.814 ± 0.451
3.215ValAsn: 3.215 ± 0.569
2.886ValPro: 2.886 ± 0.424
3.545ValGln: 3.545 ± 0.541
3.957ValArg: 3.957 ± 0.604
3.875ValSer: 3.875 ± 0.616
4.617ValThr: 4.617 ± 0.555
4.617ValVal: 4.617 ± 0.69
1.072ValTrp: 1.072 ± 0.297
2.144ValTyr: 2.144 ± 0.388
0.0ValXaa: 0.0 ± 0.0
Trp
1.319TrpAla: 1.319 ± 0.289
0.082TrpCys: 0.082 ± 0.095
0.412TrpAsp: 0.412 ± 0.179
0.412TrpGlu: 0.412 ± 0.154
0.33TrpPhe: 0.33 ± 0.204
1.072TrpGly: 1.072 ± 0.162
0.495TrpHis: 0.495 ± 0.216
0.33TrpIle: 0.33 ± 0.153
1.566TrpLys: 1.566 ± 0.329
1.649TrpLeu: 1.649 ± 0.419
0.33TrpMet: 0.33 ± 0.153
0.577TrpAsn: 0.577 ± 0.201
0.412TrpPro: 0.412 ± 0.169
0.742TrpGln: 0.742 ± 0.216
0.742TrpArg: 0.742 ± 0.188
0.907TrpSer: 0.907 ± 0.32
0.989TrpThr: 0.989 ± 0.245
0.989TrpVal: 0.989 ± 0.26
0.082TrpTrp: 0.082 ± 0.084
0.165TrpTyr: 0.165 ± 0.105
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.051TyrAla: 3.051 ± 0.691
0.495TyrCys: 0.495 ± 0.194
2.144TyrAsp: 2.144 ± 0.293
1.979TyrGlu: 1.979 ± 0.32
0.742TyrPhe: 0.742 ± 0.249
2.556TyrGly: 2.556 ± 0.426
0.412TyrHis: 0.412 ± 0.172
1.154TyrIle: 1.154 ± 0.268
1.814TyrLys: 1.814 ± 0.445
1.979TyrLeu: 1.979 ± 0.384
1.484TyrMet: 1.484 ± 0.45
1.402TyrAsn: 1.402 ± 0.429
0.742TyrPro: 0.742 ± 0.221
1.154TyrGln: 1.154 ± 0.296
2.061TyrArg: 2.061 ± 0.293
1.484TyrSer: 1.484 ± 0.336
1.484TyrThr: 1.484 ± 0.351
1.566TyrVal: 1.566 ± 0.437
0.577TyrTrp: 0.577 ± 0.208
0.412TyrTyr: 0.412 ± 0.189
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 46 proteins (12130 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski