Oxobacter pfennigii
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4273 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0P8X0T5|A0A0P8X0T5_9CLOT Electron transport complex subunit RsxG OS=Oxobacter pfennigii OX=36849 GN=rsxG_1 PE=4 SV=1
MM1 pKa = 7.46 KK2 pKa = 10.79 SMVKK6 pKa = 10.31 GFRR9 pKa = 11.84 SKK11 pKa = 10.84 LALLMALVVVFSFSLSMTAFAAIDD35 pKa = 3.42 TTVTVKK41 pKa = 10.21 FYY43 pKa = 11.24 NDD45 pKa = 3.28 TVDD48 pKa = 4.4 PDD50 pKa = 3.68 VQLWTTRR57 pKa = 11.84 TVEE60 pKa = 3.88 YY61 pKa = 10.63 DD62 pKa = 3.3 SAVPVSKK69 pKa = 10.16 PYY71 pKa = 10.97 LPGGYY76 pKa = 8.08 TDD78 pKa = 4.42 PLGGAASVYY87 pKa = 10.58 DD88 pKa = 4.71 AIFAAAEE95 pKa = 4.08 QIRR98 pKa = 11.84 ALPDD102 pKa = 3.51 EE103 pKa = 5.03 DD104 pKa = 5.48 PEE106 pKa = 5.51 IEE108 pKa = 4.51 DD109 pKa = 4.16 PPVVGWDD116 pKa = 3.38 ANPAYY121 pKa = 9.64 GDD123 pKa = 3.32 PGGYY127 pKa = 8.93 IEE129 pKa = 6.17 AIGDD133 pKa = 3.76 FVTWNDD139 pKa = 3.07 YY140 pKa = 11.22 DD141 pKa = 4.0 YY142 pKa = 11.68 DD143 pKa = 5.49 PITGHH148 pKa = 6.78 HH149 pKa = 6.12 ISEE152 pKa = 4.62 GEE154 pKa = 4.09 GWVCTVIPDD163 pKa = 4.21 GDD165 pKa = 4.09 DD166 pKa = 3.76 PYY168 pKa = 11.98 DD169 pKa = 4.51 PIQYY173 pKa = 8.22 LTAEE177 pKa = 4.13 ALEE180 pKa = 5.09 DD181 pKa = 3.34 GMEE184 pKa = 4.34 IIFRR188 pKa = 11.84 FQSYY192 pKa = 9.55 RR193 pKa = 11.84 YY194 pKa = 8.81 EE195 pKa = 3.9 WDD197 pKa = 3.45 DD198 pKa = 3.32
Molecular weight: 21.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.745
IPC_protein 3.757
Toseland 3.528
ProMoST 3.884
Dawson 3.757
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.579
Grimsley 3.439
Solomon 3.745
Lehninger 3.694
Nozaki 3.872
DTASelect 4.126
Thurlkill 3.592
EMBOSS 3.706
Sillero 3.872
Patrickios 0.629
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.8
Protein with the highest isoelectric point:
>tr|A0A0N8NTP6|A0A0N8NTP6_9CLOT High-affinity zinc uptake system ATP-binding protein ZnuC OS=Oxobacter pfennigii OX=36849 GN=znuC_1 PE=4 SV=1
MM1 pKa = 7.41 LLVLPVRR8 pKa = 11.84 LALPATQVLLALLVLPARR26 pKa = 11.84 LALPATQVLLALLVLPATQVLLALLVLPATQVLLAPLVLPAQLALPVTQVLLALLVLPARR86 pKa = 11.84 LALPATQVLLALLVLPARR104 pKa = 11.84 LALPVTQVLLALLVLPARR122 pKa = 11.84 LALPVTQVLLALLVLPARR140 pKa = 11.84 LALPATQVLLALLVLPARR158 pKa = 11.84 LALPATQVLLALLALPAQLALPATRR183 pKa = 11.84 VLLALLVLPARR194 pKa = 11.84 LALPATQVLLALLVLPARR212 pKa = 11.84 LALPATRR219 pKa = 11.84 VLLALLALPARR230 pKa = 11.84 LALPVTRR237 pKa = 11.84 VLLALLALPARR248 pKa = 11.84 LALPVLLLLVLSFPLQVDD266 pKa = 3.81 LSPRR270 pKa = 3.43
Molecular weight: 28.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.472
IPC2_protein 11.038
IPC_protein 12.647
Toseland 12.808
ProMoST 13.32
Dawson 12.808
Bjellqvist 12.808
Wikipedia 13.29
Rodwell 12.31
Grimsley 12.852
Solomon 13.32
Lehninger 13.217
Nozaki 12.808
DTASelect 12.808
Thurlkill 12.808
EMBOSS 13.32
Sillero 12.808
Patrickios 12.047
IPC_peptide 13.32
IPC2_peptide 12.31
IPC2.peptide.svr19 9.169
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4273
0
4273
1282805
30
3275
300.2
33.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.883 ± 0.038
1.174 ± 0.019
5.619 ± 0.028
6.931 ± 0.044
4.302 ± 0.028
6.954 ± 0.038
1.513 ± 0.016
9.098 ± 0.048
7.687 ± 0.041
9.127 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.917 ± 0.019
5.106 ± 0.033
3.37 ± 0.024
2.623 ± 0.022
3.901 ± 0.027
6.362 ± 0.032
5.039 ± 0.038
6.496 ± 0.033
0.818 ± 0.013
4.08 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here