Erythrobacter sp. HI0063

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Erythrobacteraceae; Erythrobacter/Porphyrobacter group; Erythrobacter; unclassified Erythrobacter

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2915 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A167IAQ3|A0A167IAQ3_9SPHN NADH-quinone oxidoreductase subunit B OS=Erythrobacter sp. HI0063 OX=1822240 GN=nuoB PE=3 SV=1
MM1 pKa = 7.79KK2 pKa = 10.02IHH4 pKa = 6.68SALTACLLLAACGSNEE20 pKa = 4.75AEE22 pKa = 4.4DD23 pKa = 4.27TVPAPADD30 pKa = 3.31VAAPSTTTDD39 pKa = 3.2TVTPAAMAGTYY50 pKa = 9.24EE51 pKa = 4.1VTLPDD56 pKa = 3.67GTVTMQTLSADD67 pKa = 3.31GTYY70 pKa = 10.34VDD72 pKa = 5.05RR73 pKa = 11.84MDD75 pKa = 5.04GEE77 pKa = 4.65VTEE80 pKa = 4.5SGTWRR85 pKa = 11.84QQGDD89 pKa = 3.81QLCYY93 pKa = 10.56APEE96 pKa = 5.02GDD98 pKa = 4.1TATEE102 pKa = 3.76QCFAGGEE109 pKa = 4.1PGPDD113 pKa = 3.04GAFEE117 pKa = 4.06MRR119 pKa = 11.84DD120 pKa = 3.49DD121 pKa = 4.74AGDD124 pKa = 3.36VTATVRR130 pKa = 11.84KK131 pKa = 9.99VDD133 pKa = 3.79AQPTAA138 pKa = 3.41

Molecular weight: 14.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A165R8D3|A0A165R8D3_9SPHN Phosphoribosylglycinamide formyltransferase OS=Erythrobacter sp. HI0063 OX=1822240 GN=purN PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.89GFFARR21 pKa = 11.84KK22 pKa = 7.42ATPGGRR28 pKa = 11.84KK29 pKa = 8.06VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 8.8RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.46NLCAA44 pKa = 4.54

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2915

0

2915

915420

41

1687

314.0

34.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.698 ± 0.065

0.804 ± 0.016

6.404 ± 0.043

6.444 ± 0.047

3.637 ± 0.031

8.718 ± 0.048

1.887 ± 0.024

4.995 ± 0.028

3.061 ± 0.036

9.812 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.459 ± 0.021

2.52 ± 0.03

5.097 ± 0.036

3.09 ± 0.024

7.37 ± 0.042

5.351 ± 0.033

5.155 ± 0.033

6.927 ± 0.032

1.401 ± 0.021

2.169 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski