Simplicispira suum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Simplicispira

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3677 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S0N5I0|A0A2S0N5I0_9BURK Toxin YhaV OS=Simplicispira suum OX=2109915 GN=C6571_18395 PE=4 SV=1
MM1 pKa = 7.64CSSDD5 pKa = 4.5SEE7 pKa = 4.63VFTYY11 pKa = 11.0NSGPLEE17 pKa = 4.37DD18 pKa = 3.61VCAASGLAFAEE29 pKa = 4.18LDD31 pKa = 3.58ADD33 pKa = 3.91PEE35 pKa = 4.03EE36 pKa = 4.41SLRR39 pKa = 11.84VIAAWYY45 pKa = 7.83EE46 pKa = 3.67LHH48 pKa = 6.66LANGGARR55 pKa = 11.84HH56 pKa = 6.56DD57 pKa = 4.78AGDD60 pKa = 3.57TAINAMSRR68 pKa = 11.84CSLSTEE74 pKa = 3.9

Molecular weight: 7.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S0N4S7|A0A2S0N4S7_9BURK Patatin OS=Simplicispira suum OX=2109915 GN=C6571_02710 PE=4 SV=1
MM1 pKa = 7.68LARR4 pKa = 11.84LGRR7 pKa = 11.84WPGLRR12 pKa = 11.84WVEE15 pKa = 3.87GRR17 pKa = 11.84VRR19 pKa = 11.84ALPPWAALAAFGVPTLLLLPIKK41 pKa = 10.47LLALWAIGNGQVLLGAAVVIAAKK64 pKa = 10.17IVGTAVVARR73 pKa = 11.84LFTLTQPALMRR84 pKa = 11.84LAWFAIFYY92 pKa = 9.04VRR94 pKa = 11.84WIALKK99 pKa = 9.1TALLARR105 pKa = 11.84ARR107 pKa = 11.84ASWPWRR113 pKa = 11.84AARR116 pKa = 11.84VLRR119 pKa = 11.84RR120 pKa = 11.84RR121 pKa = 11.84ARR123 pKa = 11.84RR124 pKa = 11.84WWRR127 pKa = 11.84RR128 pKa = 11.84TGLRR132 pKa = 3.62

Molecular weight: 14.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3677

0

3677

1198209

37

2687

325.9

35.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.215 ± 0.063

0.974 ± 0.014

5.011 ± 0.03

5.351 ± 0.036

3.488 ± 0.025

8.237 ± 0.036

2.294 ± 0.016

4.205 ± 0.03

3.187 ± 0.042

11.025 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.518 ± 0.02

2.458 ± 0.021

5.18 ± 0.029

4.282 ± 0.026

6.853 ± 0.037

5.474 ± 0.028

4.971 ± 0.024

7.627 ± 0.03

1.496 ± 0.021

2.152 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski