Deinococcus yavapaiensis KR-236
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4447 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A318S7L4|A0A318S7L4_9DEIO Tetratricopeptide repeat protein OS=Deinococcus yavapaiensis KR-236 OX=694435 GN=DES52_10569 PE=3 SV=1
MM1 pKa = 7.22 TRR3 pKa = 11.84 TIRR6 pKa = 11.84 TLALAALTLTTGLASASSVLSYY28 pKa = 10.86 VATYY32 pKa = 9.81 DD33 pKa = 3.4 AQGYY37 pKa = 7.74 YY38 pKa = 11.03 SNVATLDD45 pKa = 3.49 ANQNGVTTSLFVPASLLGLDD65 pKa = 4.5 DD66 pKa = 4.36 VSGTDD71 pKa = 3.37 EE72 pKa = 4.42 SGSVQLTAITTSVSGVSFDD91 pKa = 5.14 LNAASIASTALQDD104 pKa = 3.04 GMLRR108 pKa = 11.84 VDD110 pKa = 4.2 VIAYY114 pKa = 10.01 SDD116 pKa = 3.41 GWQGNGVYY124 pKa = 10.09 PVVLTLKK131 pKa = 9.07 NTSTGQSVDD140 pKa = 3.2 VQANVVVTNNAEE152 pKa = 3.74
Molecular weight: 15.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.728
IPC2_protein 3.719
IPC_protein 3.668
Toseland 3.439
ProMoST 3.834
Dawson 3.694
Bjellqvist 3.948
Wikipedia 3.706
Rodwell 3.503
Grimsley 3.363
Solomon 3.668
Lehninger 3.617
Nozaki 3.834
DTASelect 4.113
Thurlkill 3.541
EMBOSS 3.706
Sillero 3.795
Patrickios 0.693
IPC_peptide 3.656
IPC2_peptide 3.757
IPC2.peptide.svr19 3.789
Protein with the highest isoelectric point:
>tr|A0A318S2X6|A0A318S2X6_9DEIO Carbon-monoxide dehydrogenase small subunit OS=Deinococcus yavapaiensis KR-236 OX=694435 GN=DES52_11596 PE=4 SV=1
MM1 pKa = 7.75 LIHH4 pKa = 6.31 VLSAVIGLGPTFFSAALLRR23 pKa = 11.84 GGPSPVALRR32 pKa = 11.84 HH33 pKa = 5.17 NLALFSALSAFPKK46 pKa = 10.27 IGGTLTVSAGWRR58 pKa = 11.84 SSGVVSRR65 pKa = 11.84 GRR67 pKa = 3.21
Molecular weight: 6.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.394
IPC2_protein 10.833
IPC_protein 12.442
Toseland 12.603
ProMoST 13.115
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.193
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.974
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.137
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4447
0
4447
1404029
27
4291
315.7
34.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.041 ± 0.051
0.586 ± 0.01
5.571 ± 0.031
5.8 ± 0.038
3.646 ± 0.023
8.225 ± 0.039
2.214 ± 0.023
3.324 ± 0.032
2.73 ± 0.033
11.382 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.747 ± 0.015
2.619 ± 0.028
5.149 ± 0.027
2.935 ± 0.022
7.971 ± 0.052
5.69 ± 0.028
6.104 ± 0.044
8.612 ± 0.033
1.401 ± 0.015
2.253 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here