Dickeya phage vB_DsoM_JA29
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 318 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A384ZXM6|A0A384ZXM6_9CAUD Uncharacterized protein OS=Dickeya phage vB_DsoM_JA29 OX=2283031 GN=JA29_297 PE=4 SV=1
MM1 pKa = 7.79 SDD3 pKa = 3.11 QEE5 pKa = 4.27 QPYY8 pKa = 10.23 SRR10 pKa = 11.84 CTDD13 pKa = 3.29 CGEE16 pKa = 4.75 DD17 pKa = 3.32 IEE19 pKa = 4.51 EE20 pKa = 4.7 GEE22 pKa = 5.63 VYY24 pKa = 10.91 GEE26 pKa = 3.9 WDD28 pKa = 4.35 DD29 pKa = 5.05 LCEE32 pKa = 3.79 SCYY35 pKa = 9.1 YY36 pKa = 9.61 QRR38 pKa = 11.84 EE39 pKa = 4.16 RR40 pKa = 11.84 EE41 pKa = 4.33 GRR43 pKa = 11.84 GSS45 pKa = 3.16
Molecular weight: 5.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.885
IPC2_protein 3.948
IPC_protein 3.808
Toseland 3.643
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.681
Rodwell 3.656
Grimsley 3.567
Solomon 3.745
Lehninger 3.706
Nozaki 3.91
DTASelect 4.024
Thurlkill 3.694
EMBOSS 3.694
Sillero 3.923
Patrickios 0.223
IPC_peptide 3.757
IPC2_peptide 3.897
IPC2.peptide.svr19 3.828
Protein with the highest isoelectric point:
>tr|A0A384ZXI6|A0A384ZXI6_9CAUD Uncharacterized protein OS=Dickeya phage vB_DsoM_JA29 OX=2283031 GN=JA29_225 PE=4 SV=1
MM1 pKa = 7.34 NPLHH5 pKa = 6.96 ASQTAFYY12 pKa = 9.81 VLGSITLLAHH22 pKa = 6.88 LFSLVYY28 pKa = 9.99 RR29 pKa = 11.84 YY30 pKa = 9.26 MKK32 pKa = 9.8 IVEE35 pKa = 3.97 MRR37 pKa = 11.84 QKK39 pKa = 10.51 RR40 pKa = 11.84 RR41 pKa = 11.84 AKK43 pKa = 9.61 IAKK46 pKa = 9.42 QKK48 pKa = 9.83 TPLDD52 pKa = 3.66 KK53 pKa = 10.91 LRR55 pKa = 11.84 SRR57 pKa = 11.84 LKK59 pKa = 8.25 QAKK62 pKa = 9.3 RR63 pKa = 11.84 EE64 pKa = 3.9 AYY66 pKa = 9.64 YY67 pKa = 10.47 RR68 pKa = 11.84 PKK70 pKa = 10.75 KK71 pKa = 10.33 LL72 pKa = 3.44
Molecular weight: 8.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.272
IPC2_protein 10.321
IPC_protein 11.038
Toseland 11.111
ProMoST 10.877
Dawson 11.184
Bjellqvist 10.921
Wikipedia 11.418
Rodwell 11.52
Grimsley 11.228
Solomon 11.345
Lehninger 11.316
Nozaki 11.082
DTASelect 10.921
Thurlkill 11.111
EMBOSS 11.52
Sillero 11.125
Patrickios 11.242
IPC_peptide 11.359
IPC2_peptide 9.794
IPC2.peptide.svr19 7.861
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
318
0
318
79552
33
1944
250.2
28.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.574 ± 0.164
1.096 ± 0.056
6.592 ± 0.16
6.813 ± 0.2
4.328 ± 0.108
5.977 ± 0.166
1.825 ± 0.066
6.097 ± 0.118
6.876 ± 0.241
8.219 ± 0.143
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.491 ± 0.069
4.842 ± 0.097
4.186 ± 0.103
3.43 ± 0.07
5.291 ± 0.097
7.032 ± 0.128
5.829 ± 0.163
6.953 ± 0.11
1.057 ± 0.046
3.492 ± 0.124
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here