Mycoplasma phocirhinis
Average proteome isoelectric point is 7.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 676 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P6MTV0|A0A4P6MTV0_9MOLU HAD family phosphatase OS=Mycoplasma phocirhinis OX=142650 GN=EG856_02740 PE=4 SV=1
MM1 pKa = 7.33 NKK3 pKa = 10.21 DD4 pKa = 3.49 KK5 pKa = 11.31 NILLNTEE12 pKa = 3.81 YY13 pKa = 10.19 LTRR16 pKa = 11.84 LEE18 pKa = 3.87 QNEE21 pKa = 4.26 IYY23 pKa = 10.38 KK24 pKa = 10.78 AILNYY29 pKa = 10.05 DD30 pKa = 3.85 SKK32 pKa = 11.53 LSAIVQATKK41 pKa = 10.5 KK42 pKa = 7.77 QTISQTDD49 pKa = 3.48 YY50 pKa = 11.25 FRR52 pKa = 11.84 IKK54 pKa = 11.02 SNFNQVEE61 pKa = 4.06 DD62 pKa = 5.4 LIDD65 pKa = 4.48 DD66 pKa = 4.96 LLTISRR72 pKa = 11.84 FNYY75 pKa = 9.99 LEE77 pKa = 4.12 EE78 pKa = 4.28 VKK80 pKa = 10.55 SLYY83 pKa = 10.92 ASIVNNFNLLFTKK96 pKa = 10.35 YY97 pKa = 10.58 VIDD100 pKa = 3.84 YY101 pKa = 7.46 TASDD105 pKa = 3.82 SPDD108 pKa = 2.57 TRR110 pKa = 11.84 AYY112 pKa = 10.41 HH113 pKa = 7.12 KK114 pKa = 10.78 LDD116 pKa = 3.43 TEE118 pKa = 4.4 QQFSLTSYY126 pKa = 8.18 LTSFVIVEE134 pKa = 4.16 DD135 pKa = 3.54 KK136 pKa = 10.95 FVIFVAVDD144 pKa = 3.68 KK145 pKa = 10.54 YY146 pKa = 10.86 QYY148 pKa = 10.32 PILTEE153 pKa = 4.49 FVCSEE158 pKa = 4.25 TDD160 pKa = 4.1 FSCPADD166 pKa = 3.91 CEE168 pKa = 4.33 KK169 pKa = 10.88 SCCLVQVQAVEE180 pKa = 4.04 NSDD183 pKa = 3.34 MAEE186 pKa = 4.04 TQNITEE192 pKa = 4.62 EE193 pKa = 5.02 DD194 pKa = 4.33 YY195 pKa = 11.51 SCPTDD200 pKa = 4.27 CEE202 pKa = 4.32 KK203 pKa = 10.41 TCCVTEE209 pKa = 4.16 VQAVEE214 pKa = 4.07 NSDD217 pKa = 3.58 IEE219 pKa = 4.64 TNTNEE224 pKa = 4.12 NEE226 pKa = 4.15 TTPVEE231 pKa = 4.73 FIEE234 pKa = 4.09 EE235 pKa = 4.12 TKK237 pKa = 10.62 INEE240 pKa = 3.8 EE241 pKa = 3.94 NLILDD246 pKa = 3.96 HH247 pKa = 7.37 SDD249 pKa = 2.97 VLTQTEE255 pKa = 4.49 FNQYY259 pKa = 10.56 LDD261 pKa = 3.78 LAHH264 pKa = 5.79 YY265 pKa = 8.38 TSLPEE270 pKa = 5.1 KK271 pKa = 10.57 DD272 pKa = 4.86 LLDD275 pKa = 4.28 ALTKK279 pKa = 10.52 YY280 pKa = 8.59 SHH282 pKa = 7.03 KK283 pKa = 10.66 LEE285 pKa = 5.02 KK286 pKa = 10.53 INTNFKK292 pKa = 10.39 RR293 pKa = 11.84 QTVSQADD300 pKa = 3.75 YY301 pKa = 11.05 FRR303 pKa = 11.84 TKK305 pKa = 10.78 QNLNDD310 pKa = 3.63 VQEE313 pKa = 4.56 LVDD316 pKa = 5.76 DD317 pKa = 5.04 LLTHH321 pKa = 7.37 DD322 pKa = 4.16 SYY324 pKa = 11.98 SYY326 pKa = 10.52 KK327 pKa = 10.42 QEE329 pKa = 3.99 TQLVYY334 pKa = 11.28 DD335 pKa = 3.9 DD336 pKa = 4.03 VNRR339 pKa = 11.84 NFDD342 pKa = 4.13 NILNNLVIDD351 pKa = 3.78 YY352 pKa = 7.56 TAADD356 pKa = 3.84 TPDD359 pKa = 3.59 NNGLALLNINEE370 pKa = 4.32 NDD372 pKa = 3.3 FLNYY376 pKa = 7.14 QTSFILHH383 pKa = 5.92 EE384 pKa = 4.5 NKK386 pKa = 9.78 QVVFNVANEE395 pKa = 3.82 YY396 pKa = 10.55 SYY398 pKa = 10.94 PILSVQNLEE407 pKa = 4.27 TNSIEE412 pKa = 4.41 DD413 pKa = 3.48 SSEE416 pKa = 4.23 HH417 pKa = 5.61 EE418 pKa = 3.79 QHH420 pKa = 6.71 LMNEE424 pKa = 4.26 MLVEE428 pKa = 4.01 NSVVEE433 pKa = 4.4 PIMVDD438 pKa = 3.3 EE439 pKa = 4.68 EE440 pKa = 4.64 VIEE443 pKa = 4.58 HH444 pKa = 6.31 QDD446 pKa = 2.96 KK447 pKa = 10.44 TEE449 pKa = 3.88 NNEE452 pKa = 3.68 FVEE455 pKa = 5.3 FVQSEE460 pKa = 4.41 EE461 pKa = 4.2 VQNSCNKK468 pKa = 9.89 EE469 pKa = 3.83 EE470 pKa = 4.91 CEE472 pKa = 4.52 TNCEE476 pKa = 4.15 CASEE480 pKa = 4.16 CDD482 pKa = 3.38 ITVHH486 pKa = 5.08 EE487 pKa = 4.44 TQTFVVDD494 pKa = 3.84 SNKK497 pKa = 9.93 KK498 pKa = 9.66 LKK500 pKa = 9.85 PSRR503 pKa = 11.84 YY504 pKa = 8.96 LSKK507 pKa = 10.5 PKK509 pKa = 10.38 KK510 pKa = 9.42 FWWILSLVVVSLILVVLLILVGLRR534 pKa = 11.84 AYY536 pKa = 9.85 EE537 pKa = 4.13 IINNIDD543 pKa = 2.97 IFF545 pKa = 4.1
Molecular weight: 63.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.007
IPC2_protein 4.24
IPC_protein 4.215
Toseland 4.037
ProMoST 4.317
Dawson 4.164
Bjellqvist 4.304
Wikipedia 4.024
Rodwell 4.037
Grimsley 3.948
Solomon 4.164
Lehninger 4.113
Nozaki 4.266
DTASelect 4.431
Thurlkill 4.037
EMBOSS 4.05
Sillero 4.317
Patrickios 2.308
IPC_peptide 4.164
IPC2_peptide 4.304
IPC2.peptide.svr19 4.231
Protein with the highest isoelectric point:
>tr|A0A4P6MNY0|A0A4P6MNY0_9MOLU Cation-translocating P-type ATPase OS=Mycoplasma phocirhinis OX=142650 GN=EG856_02490 PE=4 SV=1
MM1 pKa = 7.52 ARR3 pKa = 11.84 ILNIEE8 pKa = 3.76 IPNNKK13 pKa = 8.93 RR14 pKa = 11.84 VVVSLTYY21 pKa = 10.35 IFGIGLTRR29 pKa = 11.84 SKK31 pKa = 10.93 QILAKK36 pKa = 10.84 ANINEE41 pKa = 4.33 NIRR44 pKa = 11.84 VKK46 pKa = 10.72 DD47 pKa = 3.72 LSEE50 pKa = 4.16 EE51 pKa = 3.84 QLSAIRR57 pKa = 11.84 EE58 pKa = 4.16 VARR61 pKa = 11.84 EE62 pKa = 3.89 YY63 pKa = 9.51 QTEE66 pKa = 4.25 GDD68 pKa = 3.62 LHH70 pKa = 8.28 RR71 pKa = 11.84 EE72 pKa = 3.37 VSLNIKK78 pKa = 10.05 RR79 pKa = 11.84 LMEE82 pKa = 4.14 IKK84 pKa = 10.07 CYY86 pKa = 10.37 RR87 pKa = 11.84 GMRR90 pKa = 11.84 HH91 pKa = 6.14 RR92 pKa = 11.84 KK93 pKa = 7.99 GLPVRR98 pKa = 11.84 GQSTKK103 pKa = 10.87 SNARR107 pKa = 11.84 TRR109 pKa = 11.84 KK110 pKa = 9.46 GPRR113 pKa = 11.84 KK114 pKa = 7.66 TVAGKK119 pKa = 10.41 KK120 pKa = 9.46 KK121 pKa = 10.5
Molecular weight: 13.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.308
IPC2_protein 9.853
IPC_protein 10.716
Toseland 11.082
ProMoST 10.891
Dawson 11.125
Bjellqvist 10.847
Wikipedia 11.359
Rodwell 11.389
Grimsley 11.155
Solomon 11.316
Lehninger 11.272
Nozaki 11.052
DTASelect 10.847
Thurlkill 11.052
EMBOSS 11.491
Sillero 11.067
Patrickios 11.111
IPC_peptide 11.33
IPC2_peptide 9.75
IPC2.peptide.svr19 8.621
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
676
0
676
260632
37
3640
385.6
44.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.393 ± 0.126
0.423 ± 0.024
5.418 ± 0.083
6.044 ± 0.076
5.353 ± 0.084
4.358 ± 0.095
1.529 ± 0.042
9.554 ± 0.121
9.683 ± 0.076
9.494 ± 0.089
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.611 ± 0.049
8.962 ± 0.18
2.627 ± 0.045
4.418 ± 0.079
3.007 ± 0.053
6.567 ± 0.067
5.272 ± 0.063
5.217 ± 0.073
0.92 ± 0.027
4.149 ± 0.061
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here