uncultured phage_MedDCM-OCT-S39-C11

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Krakvirus; Krakvirus S39C11

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 32 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6S4PGY8|A0A6S4PGY8_9CAUD Endonuclease OS=uncultured phage_MedDCM-OCT-S39-C11 OX=2740805 PE=4 SV=1
MM1 pKa = 7.64EE2 pKa = 5.13LQEE5 pKa = 3.89AWRR8 pKa = 11.84DD9 pKa = 3.8VIRR12 pKa = 11.84PTMYY16 pKa = 10.43RR17 pKa = 11.84EE18 pKa = 3.96LKK20 pKa = 9.01TPGNKK25 pKa = 9.42GVEE28 pKa = 4.32VVMVEE33 pKa = 3.98AAGGDD38 pKa = 3.74TPEE41 pKa = 5.2PEE43 pKa = 4.91PEE45 pKa = 3.98RR46 pKa = 11.84QPITVTTGAAWADD59 pKa = 3.67LNDD62 pKa = 3.61YY63 pKa = 10.96SIGSEE68 pKa = 4.09VFADD72 pKa = 3.74VAAYY76 pKa = 8.93TGGNPDD82 pKa = 3.22TTTYY86 pKa = 10.28RR87 pKa = 11.84YY88 pKa = 9.47RR89 pKa = 11.84WQARR93 pKa = 11.84ATADD97 pKa = 3.75DD98 pKa = 3.88GWVNGSWTNYY108 pKa = 8.89NDD110 pKa = 3.37HH111 pKa = 7.36AMEE114 pKa = 4.17VSTTIAEE121 pKa = 4.38PGQLRR126 pKa = 11.84FQCQARR132 pKa = 11.84DD133 pKa = 3.54TSVDD137 pKa = 3.06PVEE140 pKa = 4.32QVNSFAAVKK149 pKa = 9.49TVDD152 pKa = 3.31TPAALVISTPVVTGEE167 pKa = 4.37PIVGYY172 pKa = 7.94TLTCSEE178 pKa = 4.37PTVSGGVGPYY188 pKa = 9.78QLDD191 pKa = 4.01YY192 pKa = 11.23FWVDD196 pKa = 3.02EE197 pKa = 4.48SNAIVWEE204 pKa = 3.98ATYY207 pKa = 9.87MGNTTKK213 pKa = 10.47IIDD216 pKa = 3.75YY217 pKa = 10.98DD218 pKa = 3.6LGKK221 pKa = 9.29TMKK224 pKa = 10.52CLVTVTDD231 pKa = 3.09KK232 pKa = 11.43GYY234 pKa = 11.22ARR236 pKa = 11.84GEE238 pKa = 4.32STTVQSNQLGPINRR252 pKa = 11.84PTLPDD257 pKa = 3.24YY258 pKa = 10.71EE259 pKa = 4.74VYY261 pKa = 11.03VDD263 pKa = 5.05GALHH267 pKa = 7.5DD268 pKa = 5.24DD269 pKa = 4.02PSADD273 pKa = 3.03VGVGINGTVVLEE285 pKa = 4.12VRR287 pKa = 11.84PEE289 pKa = 3.77AVAYY293 pKa = 8.26PPLDD297 pKa = 3.01IGYY300 pKa = 8.76SWQVRR305 pKa = 11.84NGTGRR310 pKa = 11.84LSGGTNGTSVMYY322 pKa = 7.28MAPDD326 pKa = 3.79SAPAGALVTCTATSNDD342 pKa = 3.71ASDD345 pKa = 3.14SAYY348 pKa = 10.38AAEE351 pKa = 4.27VTILVAEE358 pKa = 4.42

Molecular weight: 38.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6S4PA68|A0A6S4PA68_9CAUD Phage single-stranded DNA-binding protein OS=uncultured phage_MedDCM-OCT-S39-C11 OX=2740805 PE=4 SV=1
MM1 pKa = 7.59TGPIPLPKK9 pKa = 9.88QYY11 pKa = 9.59PAHH14 pKa = 6.64LVLTYY19 pKa = 10.34RR20 pKa = 11.84GVRR23 pKa = 11.84YY24 pKa = 9.71RR25 pKa = 11.84PAALQLFVARR35 pKa = 11.84GGWGRR40 pKa = 11.84DD41 pKa = 3.52YY42 pKa = 11.02VTTAPQHH49 pKa = 5.76LSS51 pKa = 3.12

Molecular weight: 5.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

32

0

32

12754

51

1892

398.6

43.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.652 ± 0.509

0.761 ± 0.167

6.398 ± 0.206

5.912 ± 0.32

3.223 ± 0.292

8.272 ± 0.569

0.941 ± 0.214

4.665 ± 0.239

4.893 ± 0.327

8.272 ± 0.456

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.305 ± 0.343

4.869 ± 0.41

4.414 ± 0.29

5.018 ± 0.408

5.332 ± 0.54

7.033 ± 0.578

6.861 ± 0.595

6.5 ± 0.298

1.576 ± 0.226

3.105 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski