White spot syndrome virus (isolate Shrimp/China/Tongan/1996) (WSSV) (White spot bacilliform virus)
Average proteome isoelectric point is 7.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 524 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8VAG7|Q8VAG7_WSSVS Wsv449 OS=White spot syndrome virus (isolate Shrimp/China/Tongan/1996) OX=654913 PE=4 SV=1
MM1 pKa = 6.9 VFSWLMSDD9 pKa = 3.8 RR10 pKa = 11.84 SIFKK14 pKa = 7.94 TTNFTSTLNAGTEE27 pKa = 4.18 FSTAAAAASEE37 pKa = 4.2 VEE39 pKa = 4.35 EE40 pKa = 4.25 EE41 pKa = 4.75 DD42 pKa = 4.34 EE43 pKa = 4.07 EE44 pKa = 4.6 LLLFFLVSLMIEE56 pKa = 3.97 VSTKK60 pKa = 10.36 FEE62 pKa = 3.86 TAFFKK67 pKa = 10.51 TCFLVSVIAPIILSVLEE84 pKa = 4.73 EE85 pKa = 3.93 IASFSCLALEE95 pKa = 4.04 LAMRR99 pKa = 11.84 RR100 pKa = 11.84 SHH102 pKa = 7.23 ISLGCPCSFVINSSIFFLLSFPFEE126 pKa = 4.03 ILVTICMANTIDD138 pKa = 3.62 FSEE141 pKa = 4.42 YY142 pKa = 10.5 LSEE145 pKa = 4.61 LLCLLLMLLFSVV157 pKa = 4.54
Molecular weight: 17.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.97
IPC2_protein 4.279
IPC_protein 4.126
Toseland 3.986
ProMoST 4.215
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.884
Rodwell 3.961
Grimsley 3.91
Solomon 4.037
Lehninger 3.986
Nozaki 4.164
DTASelect 4.215
Thurlkill 3.986
EMBOSS 3.897
Sillero 4.228
Patrickios 1.914
IPC_peptide 4.05
IPC2_peptide 4.215
IPC2.peptide.svr19 4.141
Protein with the highest isoelectric point:
>tr|A0A0A0Y4F0|A0A0A0Y4F0_WSSVS Wsv405 OS=White spot syndrome virus (isolate Shrimp/China/Tongan/1996) OX=654913 PE=4 SV=1
MM1 pKa = 7.18 VARR4 pKa = 11.84 SSKK7 pKa = 8.23 TKK9 pKa = 10.2 SRR11 pKa = 11.84 RR12 pKa = 11.84 GSKK15 pKa = 9.86 KK16 pKa = 9.73 RR17 pKa = 11.84 STTAGRR23 pKa = 11.84 ISKK26 pKa = 9.98 RR27 pKa = 11.84 RR28 pKa = 11.84 SPSMKK33 pKa = 9.48 KK34 pKa = 9.83 RR35 pKa = 11.84 AGKK38 pKa = 10.17 KK39 pKa = 8.75 SSTVRR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 SSKK49 pKa = 10.18 SGKK52 pKa = 9.73 KK53 pKa = 9.68 SGARR57 pKa = 11.84 KK58 pKa = 9.36 SRR60 pKa = 11.84 RR61 pKa = 3.37
Molecular weight: 6.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.389
IPC_protein 12.954
Toseland 13.13
ProMoST 13.612
Dawson 13.13
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.91
Grimsley 13.173
Solomon 13.612
Lehninger 13.525
Nozaki 13.13
DTASelect 13.115
Thurlkill 13.13
EMBOSS 13.627
Sillero 13.13
Patrickios 12.632
IPC_peptide 13.627
IPC2_peptide 12.603
IPC2.peptide.svr19 9.24
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
524
0
524
124231
60
6077
237.1
26.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.777 ± 0.186
1.924 ± 0.09
5.113 ± 0.111
6.523 ± 0.171
4.878 ± 0.119
5.311 ± 0.246
1.996 ± 0.068
5.978 ± 0.095
6.718 ± 0.158
9.0 ± 0.159
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.82 ± 0.061
5.413 ± 0.142
4.665 ± 0.171
2.871 ± 0.133
5.198 ± 0.109
9.95 ± 0.217
6.017 ± 0.118
6.219 ± 0.081
0.933 ± 0.048
2.695 ± 0.063
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here