Vesicular stomatitis Indiana virus (strain San Juan) (VSIV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Vesiculovirus; Indiana vesiculovirus; Vesicular stomatitis Indiana virus

Average proteome isoelectric point is 7.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P03521|NCAP_VSIVA Nucleoprotein OS=Vesicular stomatitis Indiana virus (strain San Juan) OX=11285 GN=N PE=1 SV=1
MM1 pKa = 8.26DD2 pKa = 5.12NLTKK6 pKa = 10.36VRR8 pKa = 11.84EE9 pKa = 4.07YY10 pKa = 10.74LKK12 pKa = 10.75SYY14 pKa = 10.66SRR16 pKa = 11.84LDD18 pKa = 3.47QAVGEE23 pKa = 4.08IDD25 pKa = 3.96EE26 pKa = 5.27IEE28 pKa = 3.97AQRR31 pKa = 11.84AEE33 pKa = 3.95KK34 pKa = 10.72SNYY37 pKa = 9.35EE38 pKa = 4.03LFQEE42 pKa = 4.94DD43 pKa = 3.8GVEE46 pKa = 4.08EE47 pKa = 4.23HH48 pKa = 6.6TKK50 pKa = 10.39PSYY53 pKa = 10.31FQAADD58 pKa = 3.66DD59 pKa = 4.51SDD61 pKa = 4.18TEE63 pKa = 4.31SEE65 pKa = 5.06PEE67 pKa = 3.81IEE69 pKa = 4.88DD70 pKa = 3.57NQGLYY75 pKa = 10.33AQDD78 pKa = 3.9PEE80 pKa = 4.32AEE82 pKa = 4.15QVEE85 pKa = 4.84GFIQGPLDD93 pKa = 4.24DD94 pKa = 4.68YY95 pKa = 11.81ADD97 pKa = 3.85EE98 pKa = 4.35EE99 pKa = 4.7VDD101 pKa = 4.84VVFTSDD107 pKa = 3.02WKK109 pKa = 10.5PPEE112 pKa = 4.5LEE114 pKa = 3.95SDD116 pKa = 3.58EE117 pKa = 4.48HH118 pKa = 6.73GKK120 pKa = 7.3TLRR123 pKa = 11.84LTSPEE128 pKa = 4.05GLSGEE133 pKa = 4.78QKK135 pKa = 10.45SQWLSTIKK143 pKa = 10.75AVVQSAKK150 pKa = 9.7YY151 pKa = 8.96WNLAEE156 pKa = 4.46CTFEE160 pKa = 4.44ASGEE164 pKa = 4.14GVIMKK169 pKa = 9.27EE170 pKa = 3.92RR171 pKa = 11.84QITPDD176 pKa = 3.32VYY178 pKa = 11.16KK179 pKa = 9.69VTPVMNTHH187 pKa = 7.32PSQSEE192 pKa = 3.94AVSDD196 pKa = 3.56VWSLSKK202 pKa = 9.99TSMTFQPKK210 pKa = 9.52KK211 pKa = 10.78ASLQPLTISLDD222 pKa = 3.55EE223 pKa = 4.62LFSSRR228 pKa = 11.84GEE230 pKa = 4.17FISVGGDD237 pKa = 2.49GRR239 pKa = 11.84MSHH242 pKa = 6.7KK243 pKa = 10.31EE244 pKa = 3.81AILLGLRR251 pKa = 11.84YY252 pKa = 9.68KK253 pKa = 10.6KK254 pKa = 10.43LYY256 pKa = 8.53NQARR260 pKa = 11.84VKK262 pKa = 11.01YY263 pKa = 9.84SLL265 pKa = 3.74

Molecular weight: 29.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P0C2X2-2|C-2_VSIVA Isoform of P0C2X2 Isoform C of Protein C' OS=Vesicular stomatitis Indiana virus (strain San Juan) OX=11285 GN=P PE=4 SV=1
MM1 pKa = 7.22SCSKK5 pKa = 10.58RR6 pKa = 11.84MEE8 pKa = 4.21WKK10 pKa = 10.55SILSPLIFRR19 pKa = 11.84QQMILTQNLNQKK31 pKa = 10.15LKK33 pKa = 8.68TIKK36 pKa = 10.41VCMHH40 pKa = 7.56RR41 pKa = 11.84IQKK44 pKa = 10.01LSKK47 pKa = 10.56LKK49 pKa = 10.62ALYY52 pKa = 10.18RR53 pKa = 11.84GLL55 pKa = 3.84

Molecular weight: 6.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

3

9

4034

55

2109

448.2

51.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.181 ± 0.635

1.537 ± 0.305

5.85 ± 0.394

5.478 ± 0.625

4.636 ± 0.386

6.148 ± 0.341

2.9 ± 0.328

6.52 ± 0.64

6.743 ± 0.606

9.395 ± 0.612

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.198 ± 0.486

3.966 ± 0.419

5.082 ± 0.255

3.396 ± 0.424

5.057 ± 0.482

8.379 ± 0.325

5.677 ± 0.214

5.032 ± 0.277

1.958 ± 0.188

3.867 ± 0.38
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski