Prevotella veroralis F0319
Average proteome isoelectric point is 7.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3037 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C9MLU8|C9MLU8_9BACT Uncharacterized protein OS=Prevotella veroralis F0319 OX=649761 GN=HMPREF0973_00574 PE=4 SV=1
MM1 pKa = 7.95 DD2 pKa = 5.77 NISIFSIEE10 pKa = 4.03 GVDD13 pKa = 4.41 ARR15 pKa = 11.84 EE16 pKa = 3.8 ICNAQLGDD24 pKa = 3.89 VLFAPCSVCDD34 pKa = 3.68 KK35 pKa = 10.57 WKK37 pKa = 10.79 GDD39 pKa = 3.65 YY40 pKa = 10.63 PSDD43 pKa = 3.51 EE44 pKa = 4.75 FEE46 pKa = 4.19 THH48 pKa = 4.77 VNAMFDD54 pKa = 3.22
Molecular weight: 6.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.764
IPC2_protein 3.923
IPC_protein 3.821
Toseland 3.617
ProMoST 3.897
Dawson 3.821
Bjellqvist 4.062
Wikipedia 3.783
Rodwell 3.656
Grimsley 3.541
Solomon 3.795
Lehninger 3.757
Nozaki 3.961
DTASelect 4.164
Thurlkill 3.694
EMBOSS 3.795
Sillero 3.948
Patrickios 1.914
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.9
Protein with the highest isoelectric point:
>tr|C9MPC1|C9MPC1_9BACT Uncharacterized protein OS=Prevotella veroralis F0319 OX=649761 GN=HMPREF0973_01459 PE=4 SV=1
MM1 pKa = 7.49 KK2 pKa = 10.43 KK3 pKa = 10.6 AFLYY7 pKa = 9.76 LKK9 pKa = 10.65 NMPTCRR15 pKa = 11.84 DD16 pKa = 3.09 RR17 pKa = 11.84 RR18 pKa = 11.84 PRR20 pKa = 11.84 LSARR24 pKa = 11.84 ARR26 pKa = 11.84 QICNQLPRR34 pKa = 11.84 FMRR37 pKa = 11.84 TDD39 pKa = 3.39 EE40 pKa = 4.36 GVCPYY45 pKa = 9.99 FVVSFCIEE53 pKa = 3.72 RR54 pKa = 11.84 TIPISFRR61 pKa = 3.41
Molecular weight: 7.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.604
IPC_protein 10.54
Toseland 10.613
ProMoST 10.862
Dawson 10.73
Bjellqvist 10.511
Wikipedia 10.965
Rodwell 10.833
Grimsley 10.789
Solomon 10.877
Lehninger 10.847
Nozaki 10.672
DTASelect 10.482
Thurlkill 10.643
EMBOSS 11.038
Sillero 10.687
Patrickios 10.643
IPC_peptide 10.877
IPC2_peptide 9.955
IPC2.peptide.svr19 8.712
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3037
0
3037
859322
32
3063
283.0
32.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.895 ± 0.047
1.261 ± 0.021
5.625 ± 0.034
6.082 ± 0.051
4.431 ± 0.032
6.514 ± 0.044
2.188 ± 0.022
6.845 ± 0.05
7.025 ± 0.048
9.09 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.81 ± 0.023
5.155 ± 0.044
3.67 ± 0.025
3.751 ± 0.028
4.854 ± 0.039
6.189 ± 0.04
5.721 ± 0.042
6.312 ± 0.037
1.151 ± 0.019
4.43 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here