Paraglaciecola arctica BSs20135

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Paraglaciecola; Paraglaciecola arctica

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5330 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K6ZE90|K6ZE90_9ALTE Uroporphyrinogen-III synthase OS=Paraglaciecola arctica BSs20135 OX=493475 GN=hemD PE=3 SV=1
MM1 pKa = 7.27LTLFKK6 pKa = 10.72KK7 pKa = 10.52SALTLSLITTLSILGGCGSDD27 pKa = 4.34SNDD30 pKa = 3.18APEE33 pKa = 4.29LSGNVLVTVAEE44 pKa = 4.22NTTEE48 pKa = 4.0VSQYY52 pKa = 10.02TATDD56 pKa = 3.61DD57 pKa = 4.04GTEE60 pKa = 3.96LTYY63 pKa = 10.8SITGSDD69 pKa = 3.65LQLFAIDD76 pKa = 3.38NTGTVEE82 pKa = 5.49FINAPDD88 pKa = 4.1FDD90 pKa = 4.82SGDD93 pKa = 3.42TGPYY97 pKa = 9.79NFTVTVTDD105 pKa = 4.16AEE107 pKa = 4.49GLTDD111 pKa = 4.95SIDD114 pKa = 3.4VTVNVGDD121 pKa = 4.13VLDD124 pKa = 4.39TPSLALVQTVAADD137 pKa = 3.7YY138 pKa = 10.99SSSQVAYY145 pKa = 10.75LNYY148 pKa = 10.45ASQTVEE154 pKa = 3.82DD155 pKa = 4.1GFYY158 pKa = 11.12AKK160 pKa = 9.04TQSDD164 pKa = 4.57YY165 pKa = 11.35SISIYY170 pKa = 10.56QSDD173 pKa = 4.55VYY175 pKa = 11.03HH176 pKa = 6.91IGRR179 pKa = 11.84YY180 pKa = 9.27FIDD183 pKa = 4.41TISKK187 pKa = 10.64YY188 pKa = 9.49NTDD191 pKa = 3.86DD192 pKa = 3.53ASTEE196 pKa = 3.37IWSYY200 pKa = 9.72STQDD204 pKa = 4.25DD205 pKa = 4.16LDD207 pKa = 3.79STTRR211 pKa = 11.84NPYY214 pKa = 9.23TLVSLSAEE222 pKa = 3.75KK223 pKa = 10.4AYY225 pKa = 10.78LLRR228 pKa = 11.84YY229 pKa = 9.88GSDD232 pKa = 3.66KK233 pKa = 10.62IWIVNPQATDD243 pKa = 3.73FEE245 pKa = 4.83SFKK248 pKa = 10.85IGEE251 pKa = 4.79LDD253 pKa = 3.52LASYY257 pKa = 11.04VPDD260 pKa = 3.76NNTSGTPRR268 pKa = 11.84PSAASINDD276 pKa = 3.1GKK278 pKa = 11.04LFVVMQRR285 pKa = 11.84SDD287 pKa = 3.44DD288 pKa = 4.15SYY290 pKa = 11.54GSTNSTYY297 pKa = 10.96LAVFDD302 pKa = 4.19TTTDD306 pKa = 3.63SEE308 pKa = 4.65IEE310 pKa = 4.27TNADD314 pKa = 3.07ATDD317 pKa = 3.71SVMGIPLLGLNPLEE331 pKa = 4.37HH332 pKa = 6.71SVIAFEE338 pKa = 4.52DD339 pKa = 3.29KK340 pKa = 11.17VYY342 pKa = 8.98ITSRR346 pKa = 11.84NSYY349 pKa = 8.27TAASVDD355 pKa = 3.26QSMIEE360 pKa = 3.9AVNTNDD366 pKa = 3.38YY367 pKa = 11.46SLTTVLSAADD377 pKa = 3.25ITDD380 pKa = 3.28NSTKK384 pKa = 10.38FIQSAAIVSAEE395 pKa = 3.68KK396 pKa = 10.79GYY398 pKa = 10.82FFANEE403 pKa = 4.14TFFTPYY409 pKa = 10.15HH410 pKa = 6.38EE411 pKa = 4.97EE412 pKa = 3.71STLYY416 pKa = 10.34QFNPTTGEE424 pKa = 3.73ISNEE428 pKa = 3.9TVLDD432 pKa = 3.47IDD434 pKa = 4.31SNSISFIDD442 pKa = 3.27IDD444 pKa = 3.58AAGFLWVSVKK454 pKa = 10.38EE455 pKa = 4.11DD456 pKa = 3.36AAPGVEE462 pKa = 5.2IIDD465 pKa = 3.97TKK467 pKa = 11.01TNTAVVTRR475 pKa = 11.84LEE477 pKa = 4.31TQLNPSVIRR486 pKa = 11.84FIEE489 pKa = 4.17EE490 pKa = 3.52

Molecular weight: 53.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K6X8Y9|K6X8Y9_9ALTE Uncharacterized protein OS=Paraglaciecola arctica BSs20135 OX=493475 GN=GARC_0117 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.14RR12 pKa = 11.84KK13 pKa = 9.13RR14 pKa = 11.84SHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.38NGRR28 pKa = 11.84KK29 pKa = 9.48VIANRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.35GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5330

0

5330

1724099

37

2785

323.5

35.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.212 ± 0.037

0.977 ± 0.011

5.621 ± 0.037

5.837 ± 0.033

4.394 ± 0.023

6.619 ± 0.035

2.138 ± 0.018

6.821 ± 0.026

5.621 ± 0.03

10.404 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.46 ± 0.018

4.852 ± 0.029

3.746 ± 0.017

4.842 ± 0.035

3.965 ± 0.023

6.969 ± 0.031

5.444 ± 0.029

6.708 ± 0.028

1.245 ± 0.012

3.126 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski