Cronobacter virus Esp2949-1
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G1CSQ4|G1CSQ4_9CAUD Uncharacterized protein OS=Cronobacter virus Esp2949-1 OX=1073767 PE=4 SV=1
MM1 pKa = 7.58 HH2 pKa = 7.61 LPNGSQIFIGSEE14 pKa = 3.37 MGAEE18 pKa = 3.89 INATQVSKK26 pKa = 11.4 AEE28 pKa = 4.1 KK29 pKa = 9.28 PVLSVASTDD38 pKa = 3.35 GVAAGNYY45 pKa = 9.72 VIITSATWGRR55 pKa = 11.84 MVDD58 pKa = 3.04 RR59 pKa = 11.84 VLRR62 pKa = 11.84 VDD64 pKa = 4.93 SIVEE68 pKa = 4.11 DD69 pKa = 3.58 TSVTLEE75 pKa = 5.01 GIDD78 pKa = 3.46 TTDD81 pKa = 3.5 PNVFPAGTNTATFRR95 pKa = 11.84 VITSWTEE102 pKa = 3.67 IPCVQDD108 pKa = 3.73 LAQDD112 pKa = 3.49 GGEE115 pKa = 4.08 QQFYY119 pKa = 9.79 TYY121 pKa = 10.65 QCLSDD126 pKa = 4.65 DD127 pKa = 4.81 QEE129 pKa = 4.33 QQLPTFKK136 pKa = 10.68 SAVSLTYY143 pKa = 10.42 TFAHH147 pKa = 7.37 EE148 pKa = 4.62 YY149 pKa = 10.38 NNPIYY154 pKa = 10.55 PMLRR158 pKa = 11.84 KK159 pKa = 9.38 ADD161 pKa = 3.68 EE162 pKa = 4.55 SGEE165 pKa = 4.14 VTPMRR170 pKa = 11.84 MFVPKK175 pKa = 10.2 AKK177 pKa = 10.2 EE178 pKa = 3.55 MRR180 pKa = 11.84 LWAGVLSFNEE190 pKa = 4.01 IPQTSVNEE198 pKa = 4.1 MEE200 pKa = 4.49 TVSLSVSLKK209 pKa = 10.77 GKK211 pKa = 8.56 FTFLPSEE218 pKa = 4.13
Molecular weight: 24.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.55
IPC2_protein 4.533
IPC_protein 4.444
Toseland 4.279
ProMoST 4.495
Dawson 4.38
Bjellqvist 4.571
Wikipedia 4.24
Rodwell 4.279
Grimsley 4.19
Solomon 4.38
Lehninger 4.329
Nozaki 4.495
DTASelect 4.635
Thurlkill 4.291
EMBOSS 4.266
Sillero 4.546
Patrickios 3.808
IPC_peptide 4.38
IPC2_peptide 4.546
IPC2.peptide.svr19 4.475
Protein with the highest isoelectric point:
>tr|G1CSS5|G1CSS5_9CAUD Uncharacterized protein OS=Cronobacter virus Esp2949-1 OX=1073767 PE=4 SV=1
MM1 pKa = 7.42 EE2 pKa = 5.42 KK3 pKa = 10.26 SRR5 pKa = 11.84 RR6 pKa = 11.84 QVGKK10 pKa = 10.61 DD11 pKa = 2.84 KK12 pKa = 11.06 ADD14 pKa = 3.82 PLAYY18 pKa = 9.35 TSRR21 pKa = 11.84 LRR23 pKa = 11.84 SRR25 pKa = 11.84 RR26 pKa = 11.84 CAGKK30 pKa = 8.6 LTIAFFVKK38 pKa = 7.72 TTRR41 pKa = 11.84 PRR43 pKa = 11.84 SGIIFFRR50 pKa = 11.84 GGAVPRR56 pKa = 11.84 MNEE59 pKa = 3.74 GKK61 pKa = 8.88 TMNTYY66 pKa = 11.27 DD67 pKa = 4.16 LTTKK71 pKa = 10.3 NVPAFEE77 pKa = 4.27 VVEE80 pKa = 4.24 NGPDD84 pKa = 3.27 STDD87 pKa = 3.42 YY88 pKa = 11.34 VVATVKK94 pKa = 11.08 GEE96 pKa = 3.78 TAARR100 pKa = 11.84 LRR102 pKa = 11.84 ASSFQDD108 pKa = 2.87 RR109 pKa = 11.84 NGYY112 pKa = 9.47 LGYY115 pKa = 10.53 GYY117 pKa = 10.22 VIRR120 pKa = 11.84 QVAEE124 pKa = 3.77
Molecular weight: 13.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.267
IPC2_protein 9.575
IPC_protein 9.999
Toseland 10.218
ProMoST 9.94
Dawson 10.409
Bjellqvist 10.116
Wikipedia 10.613
Rodwell 10.716
Grimsley 10.496
Solomon 10.467
Lehninger 10.423
Nozaki 10.204
DTASelect 10.101
Thurlkill 10.262
EMBOSS 10.628
Sillero 10.335
Patrickios 10.379
IPC_peptide 10.452
IPC2_peptide 8.887
IPC2.peptide.svr19 8.641
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
45
0
45
12533
106
1156
278.5
30.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.399 ± 0.661
1.197 ± 0.176
6.016 ± 0.252
6.224 ± 0.327
3.654 ± 0.189
7.269 ± 0.321
1.7 ± 0.25
5.96 ± 0.18
6.271 ± 0.318
7.053 ± 0.303
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.721 ± 0.201
4.835 ± 0.299
3.67 ± 0.282
4.181 ± 0.277
5.617 ± 0.32
6.958 ± 0.362
5.37 ± 0.381
7.293 ± 0.328
1.34 ± 0.117
3.271 ± 0.213
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here