Clostridium sp. CAG:273

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1183 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7L6F5|R7L6F5_9CLOT Cytidine/deoxycytidylate deaminase OS=Clostridium sp. CAG:273 OX=1262789 GN=BN581_01034 PE=4 SV=1
MM1 pKa = 7.82AYY3 pKa = 9.75KK4 pKa = 9.23ITDD7 pKa = 3.38RR8 pKa = 11.84CISCGACAAEE18 pKa = 4.71CPVNCISEE26 pKa = 4.39GDD28 pKa = 3.48GKK30 pKa = 11.08YY31 pKa = 10.41EE32 pKa = 4.07IDD34 pKa = 3.4ASQCISCGTCAGVCPVEE51 pKa = 4.66APEE54 pKa = 4.5PDD56 pKa = 3.43EE57 pKa = 4.15EE58 pKa = 4.33

Molecular weight: 6.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7L5T3|R7L5T3_9CLOT Ribonuclease Y OS=Clostridium sp. CAG:273 OX=1262789 GN=rny PE=3 SV=1
MM1 pKa = 7.83AKK3 pKa = 9.58TSLKK7 pKa = 10.47VKK9 pKa = 7.57QQRR12 pKa = 11.84TQKK15 pKa = 10.65YY16 pKa = 6.33KK17 pKa = 7.88TRR19 pKa = 11.84EE20 pKa = 3.74YY21 pKa = 10.71NRR23 pKa = 11.84CKK25 pKa = 10.14ICGRR29 pKa = 11.84PHH31 pKa = 7.33AYY33 pKa = 8.2IRR35 pKa = 11.84KK36 pKa = 8.97YY37 pKa = 10.49GICRR41 pKa = 11.84LCFRR45 pKa = 11.84KK46 pKa = 9.51LAHH49 pKa = 6.45NGEE52 pKa = 4.13IPGVKK57 pKa = 9.32KK58 pKa = 10.94ASWW61 pKa = 3.03

Molecular weight: 7.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1183

0

1183

354036

29

2609

299.3

33.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.316 ± 0.058

1.075 ± 0.027

5.489 ± 0.061

8.164 ± 0.085

3.799 ± 0.045

5.841 ± 0.071

1.191 ± 0.024

10.284 ± 0.106

9.81 ± 0.082

8.31 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.447 ± 0.031

7.269 ± 0.086

2.635 ± 0.044

2.666 ± 0.033

3.306 ± 0.052

5.641 ± 0.061

5.498 ± 0.059

6.247 ± 0.055

0.618 ± 0.021

4.375 ± 0.057
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski