Halomonas sp. 1513

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halomonas; unclassified Halomonas

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3636 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8R8D3|A0A1P8R8D3_9GAMM Transglut_core3 domain-containing protein OS=Halomonas sp. 1513 OX=1883416 GN=BWR19_07295 PE=4 SV=1
MM1 pKa = 7.48SSAEE5 pKa = 4.21SFVPRR10 pKa = 11.84PMFLSDD16 pKa = 3.14SAQARR21 pKa = 11.84LRR23 pKa = 11.84EE24 pKa = 4.4LINEE28 pKa = 4.26EE29 pKa = 4.18GNPTLKK35 pKa = 10.59LRR37 pKa = 11.84VYY39 pKa = 8.88VTGGGCSGFQYY50 pKa = 10.96GFDD53 pKa = 4.42FADD56 pKa = 4.09SMADD60 pKa = 3.66DD61 pKa = 4.85DD62 pKa = 4.85TLIDD66 pKa = 3.93YY67 pKa = 10.73DD68 pKa = 4.39DD69 pKa = 3.57VAMIVDD75 pKa = 4.25PLSYY79 pKa = 10.34QYY81 pKa = 11.59LVGSTVDD88 pKa = 4.17FEE90 pKa = 4.43QGLAGARR97 pKa = 11.84FVIQNPNATTTCGCGASFMVV117 pKa = 4.17

Molecular weight: 12.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8R8F3|A0A1P8R8F3_9GAMM 2-keto-3-deoxy-galactonokinase OS=Halomonas sp. 1513 OX=1883416 GN=BWR19_03590 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.34RR12 pKa = 11.84KK13 pKa = 9.1RR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3636

0

3636

1211598

23

8572

333.2

36.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.872 ± 0.056

0.923 ± 0.014

5.944 ± 0.048

6.421 ± 0.041

3.402 ± 0.027

8.28 ± 0.041

2.428 ± 0.024

4.603 ± 0.03

2.37 ± 0.034

11.748 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.473 ± 0.023

2.371 ± 0.026

4.953 ± 0.038

3.92 ± 0.028

7.293 ± 0.049

5.389 ± 0.031

4.671 ± 0.038

7.162 ± 0.038

1.474 ± 0.022

2.303 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski