Amino acid dipepetide frequency for Sphingomonas sp. NFR15

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.836AlaAla: 21.836 ± 0.22
1.083AlaCys: 1.083 ± 0.028
8.171AlaAsp: 8.171 ± 0.092
7.512AlaGlu: 7.512 ± 0.089
4.611AlaPhe: 4.611 ± 0.067
12.257AlaGly: 12.257 ± 0.126
2.555AlaHis: 2.555 ± 0.046
7.148AlaIle: 7.148 ± 0.071
4.05AlaLys: 4.05 ± 0.065
15.247AlaLeu: 15.247 ± 0.142
3.732AlaMet: 3.732 ± 0.061
3.186AlaAsn: 3.186 ± 0.056
7.394AlaPro: 7.394 ± 0.098
4.369AlaGln: 4.369 ± 0.065
11.143AlaArg: 11.143 ± 0.13
6.593AlaSer: 6.593 ± 0.063
7.615AlaThr: 7.615 ± 0.079
9.295AlaVal: 9.295 ± 0.104
1.823AlaTrp: 1.823 ± 0.041
2.674AlaTyr: 2.674 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
1.031CysAla: 1.031 ± 0.027
0.083CysCys: 0.083 ± 0.008
0.474CysAsp: 0.474 ± 0.02
0.345CysGlu: 0.345 ± 0.015
0.27CysPhe: 0.27 ± 0.015
0.813CysGly: 0.813 ± 0.024
0.181CysHis: 0.181 ± 0.011
0.3CysIle: 0.3 ± 0.015
0.13CysLys: 0.13 ± 0.01
0.612CysLeu: 0.612 ± 0.022
0.12CysMet: 0.12 ± 0.008
0.148CysAsn: 0.148 ± 0.011
0.392CysPro: 0.392 ± 0.018
0.166CysGln: 0.166 ± 0.012
0.514CysArg: 0.514 ± 0.021
0.362CysSer: 0.362 ± 0.019
0.364CysThr: 0.364 ± 0.017
0.518CysVal: 0.518 ± 0.019
0.102CysTrp: 0.102 ± 0.01
0.169CysTyr: 0.169 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
8.713AspAla: 8.713 ± 0.096
0.43AspCys: 0.43 ± 0.019
3.302AspAsp: 3.302 ± 0.06
2.93AspGlu: 2.93 ± 0.062
2.191AspPhe: 2.191 ± 0.044
5.635AspGly: 5.635 ± 0.061
1.353AspHis: 1.353 ± 0.039
2.836AspIle: 2.836 ± 0.046
1.475AspLys: 1.475 ± 0.039
6.164AspLeu: 6.164 ± 0.076
1.228AspMet: 1.228 ± 0.031
1.206AspAsn: 1.206 ± 0.033
3.872AspPro: 3.872 ± 0.051
1.705AspGln: 1.705 ± 0.037
4.906AspArg: 4.906 ± 0.058
2.26AspSer: 2.26 ± 0.046
3.051AspThr: 3.051 ± 0.051
4.323AspVal: 4.323 ± 0.064
1.098AspTrp: 1.098 ± 0.029
1.573AspTyr: 1.573 ± 0.038
0.0AspXaa: 0.0 ± 0.0
Glu
7.251GluAla: 7.251 ± 0.093
0.266GluCys: 0.266 ± 0.014
2.589GluAsp: 2.589 ± 0.051
2.248GluGlu: 2.248 ± 0.054
1.311GluPhe: 1.311 ± 0.03
3.842GluGly: 3.842 ± 0.059
1.1GluHis: 1.1 ± 0.032
2.762GluIle: 2.762 ± 0.046
1.453GluLys: 1.453 ± 0.036
4.251GluLeu: 4.251 ± 0.062
1.225GluMet: 1.225 ± 0.035
1.099GluAsn: 1.099 ± 0.035
2.463GluPro: 2.463 ± 0.051
1.82GluGln: 1.82 ± 0.036
4.839GluArg: 4.839 ± 0.066
2.05GluSer: 2.05 ± 0.037
3.232GluThr: 3.232 ± 0.048
3.189GluVal: 3.189 ± 0.053
0.661GluTrp: 0.661 ± 0.022
0.896GluTyr: 0.896 ± 0.032
0.0GluXaa: 0.0 ± 0.0
Phe
5.111PheAla: 5.111 ± 0.067
0.291PheCys: 0.291 ± 0.013
2.762PheAsp: 2.762 ± 0.046
1.953PheGlu: 1.953 ± 0.038
1.234PhePhe: 1.234 ± 0.031
3.676PheGly: 3.676 ± 0.06
0.731PheHis: 0.731 ± 0.021
1.31PheIle: 1.31 ± 0.032
0.817PheLys: 0.817 ± 0.03
2.994PheLeu: 2.994 ± 0.048
0.648PheMet: 0.648 ± 0.019
0.972PheAsn: 0.972 ± 0.03
1.49PhePro: 1.49 ± 0.032
0.882PheGln: 0.882 ± 0.025
2.282PheArg: 2.282 ± 0.041
1.889PheSer: 1.889 ± 0.038
2.089PheThr: 2.089 ± 0.043
2.669PheVal: 2.669 ± 0.049
0.503PheTrp: 0.503 ± 0.02
0.937PheTyr: 0.937 ± 0.025
0.0PheXaa: 0.0 ± 0.0
Gly
11.239GlyAla: 11.239 ± 0.116
0.752GlyCys: 0.752 ± 0.025
5.053GlyAsp: 5.053 ± 0.058
4.342GlyGlu: 4.342 ± 0.07
3.774GlyPhe: 3.774 ± 0.057
8.473GlyGly: 8.473 ± 0.108
1.902GlyHis: 1.902 ± 0.038
4.371GlyIle: 4.371 ± 0.061
3.002GlyLys: 3.002 ± 0.055
8.509GlyLeu: 8.509 ± 0.083
2.082GlyMet: 2.082 ± 0.041
2.167GlyAsn: 2.167 ± 0.05
3.591GlyPro: 3.591 ± 0.054
2.704GlyGln: 2.704 ± 0.047
6.528GlyArg: 6.528 ± 0.079
4.593GlySer: 4.593 ± 0.067
5.233GlyThr: 5.233 ± 0.067
6.843GlyVal: 6.843 ± 0.074
1.749GlyTrp: 1.749 ± 0.044
2.437GlyTyr: 2.437 ± 0.046
0.0GlyXaa: 0.0 ± 0.0
His
2.745HisAla: 2.745 ± 0.049
0.184HisCys: 0.184 ± 0.011
1.328HisAsp: 1.328 ± 0.034
0.895HisGlu: 0.895 ± 0.026
0.805HisPhe: 0.805 ± 0.027
2.058HisGly: 2.058 ± 0.038
0.562HisHis: 0.562 ± 0.023
0.906HisIle: 0.906 ± 0.027
0.389HisLys: 0.389 ± 0.017
1.986HisLeu: 1.986 ± 0.039
0.399HisMet: 0.399 ± 0.018
0.431HisAsn: 0.431 ± 0.017
1.334HisPro: 1.334 ± 0.036
0.49HisGln: 0.49 ± 0.019
1.524HisArg: 1.524 ± 0.037
0.878HisSer: 0.878 ± 0.023
0.821HisThr: 0.821 ± 0.024
1.551HisVal: 1.551 ± 0.036
0.376HisTrp: 0.376 ± 0.017
0.579HisTyr: 0.579 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
8.272IleAla: 8.272 ± 0.09
0.327IleCys: 0.327 ± 0.014
4.083IleAsp: 4.083 ± 0.049
3.293IleGlu: 3.293 ± 0.059
1.378IlePhe: 1.378 ± 0.032
5.229IleGly: 5.229 ± 0.066
0.86IleHis: 0.86 ± 0.025
1.701IleIle: 1.701 ± 0.039
1.131IleLys: 1.131 ± 0.034
3.892IleLeu: 3.892 ± 0.057
0.69IleMet: 0.69 ± 0.024
1.226IleAsn: 1.226 ± 0.035
2.094IlePro: 2.094 ± 0.04
1.038IleGln: 1.038 ± 0.032
3.03IleArg: 3.03 ± 0.049
2.331IleSer: 2.331 ± 0.043
2.649IleThr: 2.649 ± 0.037
4.437IleVal: 4.437 ± 0.061
0.535IleTrp: 0.535 ± 0.022
0.93IleTyr: 0.93 ± 0.027
0.0IleXaa: 0.0 ± 0.0
Lys
3.766LysAla: 3.766 ± 0.065
0.125LysCys: 0.125 ± 0.01
1.314LysAsp: 1.314 ± 0.035
0.898LysGlu: 0.898 ± 0.028
0.775LysPhe: 0.775 ± 0.025
2.299LysGly: 2.299 ± 0.048
0.505LysHis: 0.505 ± 0.02
1.359LysIle: 1.359 ± 0.036
0.822LysLys: 0.822 ± 0.036
2.894LysLeu: 2.894 ± 0.049
0.63LysMet: 0.63 ± 0.021
0.616LysAsn: 0.616 ± 0.021
2.055LysPro: 2.055 ± 0.042
0.811LysGln: 0.811 ± 0.026
2.133LysArg: 2.133 ± 0.044
1.31LysSer: 1.31 ± 0.031
1.677LysThr: 1.677 ± 0.041
1.938LysVal: 1.938 ± 0.043
0.326LysTrp: 0.326 ± 0.014
0.56LysTyr: 0.56 ± 0.019
0.0LysXaa: 0.0 ± 0.0
Leu
15.034LeuAla: 15.034 ± 0.126
0.733LeuCys: 0.733 ± 0.026
6.483LeuAsp: 6.483 ± 0.073
4.217LeuGlu: 4.217 ± 0.063
3.531LeuPhe: 3.531 ± 0.062
8.535LeuGly: 8.535 ± 0.078
1.864LeuHis: 1.864 ± 0.037
4.635LeuIle: 4.635 ± 0.059
2.719LeuLys: 2.719 ± 0.046
9.308LeuLeu: 9.308 ± 0.111
1.945LeuMet: 1.945 ± 0.039
2.245LeuAsn: 2.245 ± 0.045
5.801LeuPro: 5.801 ± 0.076
2.356LeuGln: 2.356 ± 0.047
7.28LeuArg: 7.28 ± 0.084
5.798LeuSer: 5.798 ± 0.061
5.596LeuThr: 5.596 ± 0.064
7.332LeuVal: 7.332 ± 0.084
1.309LeuTrp: 1.309 ± 0.037
2.057LeuTyr: 2.057 ± 0.044
0.0LeuXaa: 0.0 ± 0.0
Met
2.873MetAla: 2.873 ± 0.054
0.125MetCys: 0.125 ± 0.011
0.887MetAsp: 0.887 ± 0.025
0.801MetGlu: 0.801 ± 0.029
0.689MetPhe: 0.689 ± 0.022
1.464MetGly: 1.464 ± 0.033
0.41MetHis: 0.41 ± 0.016
1.252MetIle: 1.252 ± 0.032
0.773MetLys: 0.773 ± 0.026
2.524MetLeu: 2.524 ± 0.046
0.557MetMet: 0.557 ± 0.024
0.566MetAsn: 0.566 ± 0.017
1.463MetPro: 1.463 ± 0.032
0.567MetGln: 0.567 ± 0.023
1.801MetArg: 1.801 ± 0.045
1.349MetSer: 1.349 ± 0.034
1.662MetThr: 1.662 ± 0.036
1.567MetVal: 1.567 ± 0.039
0.19MetTrp: 0.19 ± 0.011
0.271MetTyr: 0.271 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
3.298AsnAla: 3.298 ± 0.053
0.188AsnCys: 0.188 ± 0.011
1.301AsnAsp: 1.301 ± 0.036
0.929AsnGlu: 0.929 ± 0.025
0.851AsnPhe: 0.851 ± 0.03
2.354AsnGly: 2.354 ± 0.051
0.466AsnHis: 0.466 ± 0.019
1.173AsnIle: 1.173 ± 0.035
0.555AsnLys: 0.555 ± 0.021
2.331AsnLeu: 2.331 ± 0.043
0.449AsnMet: 0.449 ± 0.017
0.608AsnAsn: 0.608 ± 0.026
1.736AsnPro: 1.736 ± 0.039
0.709AsnGln: 0.709 ± 0.025
1.697AsnArg: 1.697 ± 0.04
1.071AsnSer: 1.071 ± 0.034
1.273AsnThr: 1.273 ± 0.034
1.835AsnVal: 1.835 ± 0.041
0.376AsnTrp: 0.376 ± 0.017
0.676AsnTyr: 0.676 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
7.968ProAla: 7.968 ± 0.108
0.297ProCys: 0.297 ± 0.015
3.855ProAsp: 3.855 ± 0.052
3.263ProGlu: 3.263 ± 0.048
1.966ProPhe: 1.966 ± 0.04
5.216ProGly: 5.216 ± 0.07
1.112ProHis: 1.112 ± 0.03
2.584ProIle: 2.584 ± 0.042
1.492ProLys: 1.492 ± 0.041
5.13ProLeu: 5.13 ± 0.062
1.097ProMet: 1.097 ± 0.034
1.336ProAsn: 1.336 ± 0.035
3.046ProPro: 3.046 ± 0.065
1.537ProGln: 1.537 ± 0.034
3.354ProArg: 3.354 ± 0.052
2.707ProSer: 2.707 ± 0.045
2.743ProThr: 2.743 ± 0.043
4.538ProVal: 4.538 ± 0.063
0.722ProTrp: 0.722 ± 0.025
1.136ProTyr: 1.136 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
4.118GlnAla: 4.118 ± 0.069
0.169GlnCys: 0.169 ± 0.011
1.318GlnAsp: 1.318 ± 0.031
1.012GlnGlu: 1.012 ± 0.027
0.99GlnPhe: 0.99 ± 0.028
2.269GlnGly: 2.269 ± 0.044
0.65GlnHis: 0.65 ± 0.021
1.688GlnIle: 1.688 ± 0.033
0.723GlnLys: 0.723 ± 0.024
2.658GlnLeu: 2.658 ± 0.047
0.712GlnMet: 0.712 ± 0.021
0.712GlnAsn: 0.712 ± 0.027
1.775GlnPro: 1.775 ± 0.039
1.08GlnGln: 1.08 ± 0.043
2.375GlnArg: 2.375 ± 0.048
1.615GlnSer: 1.615 ± 0.033
1.677GlnThr: 1.677 ± 0.045
2.13GlnVal: 2.13 ± 0.041
0.38GlnTrp: 0.38 ± 0.016
0.653GlnTyr: 0.653 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
10.171ArgAla: 10.171 ± 0.115
0.489ArgCys: 0.489 ± 0.021
4.687ArgAsp: 4.687 ± 0.063
3.797ArgGlu: 3.797 ± 0.064
3.188ArgPhe: 3.188 ± 0.047
5.672ArgGly: 5.672 ± 0.073
1.72ArgHis: 1.72 ± 0.042
4.248ArgIle: 4.248 ± 0.052
1.877ArgLys: 1.877 ± 0.04
8.3ArgLeu: 8.3 ± 0.097
1.846ArgMet: 1.846 ± 0.035
1.694ArgAsn: 1.694 ± 0.039
3.856ArgPro: 3.856 ± 0.055
2.289ArgGln: 2.289 ± 0.045
6.059ArgArg: 6.059 ± 0.082
3.595ArgSer: 3.595 ± 0.056
3.802ArgThr: 3.802 ± 0.053
5.276ArgVal: 5.276 ± 0.062
1.282ArgTrp: 1.282 ± 0.027
2.037ArgTyr: 2.037 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
6.641SerAla: 6.641 ± 0.071
0.368SerCys: 0.368 ± 0.017
2.994SerAsp: 2.994 ± 0.045
2.25SerGlu: 2.25 ± 0.042
2.043SerPhe: 2.043 ± 0.04
5.327SerGly: 5.327 ± 0.076
0.979SerHis: 0.979 ± 0.029
2.453SerIle: 2.453 ± 0.042
1.244SerLys: 1.244 ± 0.038
4.76SerLeu: 4.76 ± 0.064
0.978SerMet: 0.978 ± 0.027
1.295SerAsn: 1.295 ± 0.04
2.805SerPro: 2.805 ± 0.045
1.368SerGln: 1.368 ± 0.031
3.408SerArg: 3.408 ± 0.054
2.476SerSer: 2.476 ± 0.052
2.599SerThr: 2.599 ± 0.046
3.866SerVal: 3.866 ± 0.054
0.736SerTrp: 0.736 ± 0.023
1.386SerTyr: 1.386 ± 0.037
0.0SerXaa: 0.0 ± 0.0
Thr
7.069ThrAla: 7.069 ± 0.077
0.359ThrCys: 0.359 ± 0.018
3.025ThrAsp: 3.025 ± 0.049
2.261ThrGlu: 2.261 ± 0.043
1.898ThrPhe: 1.898 ± 0.036
5.475ThrGly: 5.475 ± 0.065
1.063ThrHis: 1.063 ± 0.03
3.302ThrIle: 3.302 ± 0.051
1.298ThrLys: 1.298 ± 0.034
6.3ThrLeu: 6.3 ± 0.082
1.178ThrMet: 1.178 ± 0.028
1.396ThrAsn: 1.396 ± 0.041
3.864ThrPro: 3.864 ± 0.061
1.499ThrGln: 1.499 ± 0.033
3.84ThrArg: 3.84 ± 0.05
2.772ThrSer: 2.772 ± 0.049
3.075ThrThr: 3.075 ± 0.056
4.446ThrVal: 4.446 ± 0.063
0.718ThrTrp: 0.718 ± 0.024
1.25ThrTyr: 1.25 ± 0.039
0.0ThrXaa: 0.0 ± 0.0
Val
10.803ValAla: 10.803 ± 0.105
0.515ValCys: 0.515 ± 0.022
4.284ValAsp: 4.284 ± 0.057
3.991ValGlu: 3.991 ± 0.062
2.317ValPhe: 2.317 ± 0.046
5.595ValGly: 5.595 ± 0.077
1.302ValHis: 1.302 ± 0.03
3.659ValIle: 3.659 ± 0.054
1.899ValLys: 1.899 ± 0.041
7.008ValLeu: 7.008 ± 0.077
1.591ValMet: 1.591 ± 0.039
1.925ValAsn: 1.925 ± 0.043
4.214ValPro: 4.214 ± 0.059
1.98ValGln: 1.98 ± 0.041
5.655ValArg: 5.655 ± 0.068
4.168ValSer: 4.168 ± 0.057
4.958ValThr: 4.958 ± 0.065
5.546ValVal: 5.546 ± 0.085
0.959ValTrp: 0.959 ± 0.026
1.399ValTyr: 1.399 ± 0.033
0.0ValXaa: 0.0 ± 0.0
Trp
1.474TrpAla: 1.474 ± 0.039
0.128TrpCys: 0.128 ± 0.01
0.728TrpAsp: 0.728 ± 0.026
0.531TrpGlu: 0.531 ± 0.019
0.583TrpPhe: 0.583 ± 0.022
0.954TrpGly: 0.954 ± 0.027
0.381TrpHis: 0.381 ± 0.016
0.676TrpIle: 0.676 ± 0.027
0.41TrpLys: 0.41 ± 0.018
1.788TrpLeu: 1.788 ± 0.039
0.328TrpMet: 0.328 ± 0.016
0.43TrpAsn: 0.43 ± 0.021
0.742TrpPro: 0.742 ± 0.026
0.594TrpGln: 0.594 ± 0.024
1.504TrpArg: 1.504 ± 0.038
0.978TrpSer: 0.978 ± 0.032
0.837TrpThr: 0.837 ± 0.025
0.838TrpVal: 0.838 ± 0.03
0.275TrpTrp: 0.275 ± 0.014
0.304TrpTyr: 0.304 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.88TyrAla: 2.88 ± 0.043
0.185TyrCys: 0.185 ± 0.012
1.615TyrAsp: 1.615 ± 0.039
1.081TyrGlu: 1.081 ± 0.031
0.834TyrPhe: 0.834 ± 0.028
2.201TyrGly: 2.201 ± 0.047
0.542TyrHis: 0.542 ± 0.02
0.764TyrIle: 0.764 ± 0.023
0.554TyrLys: 0.554 ± 0.026
2.122TyrLeu: 2.122 ± 0.041
0.358TyrMet: 0.358 ± 0.017
0.616TyrAsn: 0.616 ± 0.027
1.112TyrPro: 1.112 ± 0.026
0.743TyrGln: 0.743 ± 0.025
2.012TyrArg: 2.012 ± 0.035
1.181TyrSer: 1.181 ± 0.038
1.136TyrThr: 1.136 ± 0.033
1.63TyrVal: 1.63 ± 0.036
0.362TyrTrp: 0.362 ± 0.017
0.669TyrTyr: 0.669 ± 0.025
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4025 proteins (1327836 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski