Proteus phage PM 93
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0U2ZR64|A0A0U2ZR64_9CAUD Membrane protein OS=Proteus phage PM 93 OX=1560284 GN=PM93_0044 PE=4 SV=1
MM1 pKa = 7.69 LNRR4 pKa = 11.84 INIHH8 pKa = 6.43 LKK10 pKa = 10.48 LLSSKK15 pKa = 10.74 KK16 pKa = 9.84 DD17 pKa = 3.32 VYY19 pKa = 10.91 FNKK22 pKa = 9.5 VWSTKK27 pKa = 10.0 QGDD30 pKa = 3.73 YY31 pKa = 11.1 PFIIGIDD38 pKa = 3.37 IDD40 pKa = 3.65 GTMYY44 pKa = 11.04 YY45 pKa = 9.83 PDD47 pKa = 4.89 GDD49 pKa = 4.21 TMVSALNQIEE59 pKa = 4.16 LAYY62 pKa = 10.65 SKK64 pKa = 9.05 WHH66 pKa = 6.3 YY67 pKa = 9.25 IQEE70 pKa = 4.22 VLKK73 pKa = 10.1 DD74 pKa = 3.19 TFKK77 pKa = 10.86 RR78 pKa = 11.84 DD79 pKa = 3.47 KK80 pKa = 10.33 EE81 pKa = 4.19 CLYY84 pKa = 10.26 LTFQPIIEE92 pKa = 4.16 LHH94 pKa = 6.09 YY95 pKa = 11.2 DD96 pKa = 3.58 DD97 pKa = 6.29 GSVKK101 pKa = 10.49 EE102 pKa = 5.39 LDD104 pKa = 4.08 TKK106 pKa = 11.44 DD107 pKa = 3.45 DD108 pKa = 3.43 WFSFDD113 pKa = 2.67 WHH115 pKa = 6.67 EE116 pKa = 4.6 GYY118 pKa = 10.33 DD119 pKa = 3.29 WSLEE123 pKa = 3.84 EE124 pKa = 5.27 RR125 pKa = 11.84 LNQLFFEE132 pKa = 4.62 TEE134 pKa = 3.88 QAGEE138 pKa = 3.91 ALGDD142 pKa = 3.61 NVEE145 pKa = 5.47 DD146 pKa = 3.9 LEE148 pKa = 4.47 EE149 pKa = 4.09 WLEE152 pKa = 4.2 SNPLIWSWDD161 pKa = 3.74 NVSMSS166 pKa = 3.28
Molecular weight: 19.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.257
IPC2_protein 4.24
IPC_protein 4.202
Toseland 4.012
ProMoST 4.329
Dawson 4.164
Bjellqvist 4.317
Wikipedia 4.075
Rodwell 4.024
Grimsley 3.923
Solomon 4.164
Lehninger 4.113
Nozaki 4.279
DTASelect 4.482
Thurlkill 4.037
EMBOSS 4.088
Sillero 4.317
Patrickios 3.439
IPC_peptide 4.164
IPC2_peptide 4.304
IPC2.peptide.svr19 4.227
Protein with the highest isoelectric point:
>tr|A0A0U2ZJL6|A0A0U2ZJL6_9CAUD Uncharacterized protein OS=Proteus phage PM 93 OX=1560284 GN=PM93_0041 PE=4 SV=1
MM1 pKa = 7.81 RR2 pKa = 11.84 YY3 pKa = 10.52 DD4 pKa = 3.26 MKK6 pKa = 10.76 LNKK9 pKa = 8.86 MRR11 pKa = 11.84 KK12 pKa = 4.62 TAKK15 pKa = 10.36 YY16 pKa = 8.45 VMCYY20 pKa = 8.11 GARR23 pKa = 11.84 YY24 pKa = 6.8 EE25 pKa = 4.17 TFEE28 pKa = 6.1 AILLAKK34 pKa = 9.63 KK35 pKa = 10.09 AKK37 pKa = 9.55 KK38 pKa = 10.14 RR39 pKa = 11.84 YY40 pKa = 8.82 IKK42 pKa = 10.41 KK43 pKa = 9.87 AWQQAKK49 pKa = 10.64 ADD51 pKa = 4.12 LANMGSYY58 pKa = 7.64 GTPKK62 pKa = 10.44 AVLRR66 pKa = 11.84 GADD69 pKa = 3.31 RR70 pKa = 11.84 IMSYY74 pKa = 10.63 RR75 pKa = 11.84 KK76 pKa = 9.97 VYY78 pKa = 10.82 SGDD81 pKa = 3.02 IANFIIFDD89 pKa = 4.08 EE90 pKa = 4.46 VQHH93 pKa = 6.88 AINN96 pKa = 3.99
Molecular weight: 11.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.166
IPC2_protein 9.545
IPC_protein 9.545
Toseland 10.087
ProMoST 9.78
Dawson 10.292
Bjellqvist 9.955
Wikipedia 10.467
Rodwell 10.789
Grimsley 10.365
Solomon 10.321
Lehninger 10.277
Nozaki 10.072
DTASelect 9.955
Thurlkill 10.145
EMBOSS 10.496
Sillero 10.204
Patrickios 10.423
IPC_peptide 10.321
IPC2_peptide 8.58
IPC2.peptide.svr19 8.499
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
47
0
47
13765
54
1273
292.9
32.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.679 ± 0.499
1.075 ± 0.164
6.284 ± 0.248
7.098 ± 0.287
3.908 ± 0.231
7.388 ± 0.263
1.983 ± 0.199
5.957 ± 0.223
6.923 ± 0.267
8.231 ± 0.229
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.146 ± 0.184
4.795 ± 0.262
3.226 ± 0.154
4.221 ± 0.281
5.231 ± 0.275
6.059 ± 0.316
5.311 ± 0.304
6.698 ± 0.27
1.366 ± 0.122
3.422 ± 0.22
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here