Vibrio phage VspSw_1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Pogseptimavirus; Vibrio virus VspSw1

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 151 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411BKP5|A0A411BKP5_9CAUD Deoxynucleoside-5'-monophosphatase OS=Vibrio phage VspSw_1 OX=2484249 GN=VspSw1_142 PE=4 SV=1
MM1 pKa = 7.55FNQKK5 pKa = 10.3DD6 pKa = 3.5KK7 pKa = 11.76ANLVTLDD14 pKa = 3.99YY15 pKa = 10.95ILAEE19 pKa = 4.19LSMLSATDD27 pKa = 4.2DD28 pKa = 4.03PSDD31 pKa = 3.8TVWYY35 pKa = 9.56IDD37 pKa = 3.43SDD39 pKa = 3.71EE40 pKa = 4.45SLLVTVHH47 pKa = 6.59PKK49 pKa = 10.38YY50 pKa = 11.13GLVFYY55 pKa = 10.38NDD57 pKa = 3.83DD58 pKa = 3.18MHH60 pKa = 7.46TIGIYY65 pKa = 10.42DD66 pKa = 3.95KK67 pKa = 9.91EE68 pKa = 4.29QEE70 pKa = 4.38SEE72 pKa = 4.34ILDD75 pKa = 4.78LLDD78 pKa = 3.93DD79 pKa = 4.08WSIYY83 pKa = 10.42SDD85 pKa = 3.34SKK87 pKa = 9.17VWRR90 pKa = 11.84EE91 pKa = 3.68IGIDD95 pKa = 3.4QIDD98 pKa = 3.93YY99 pKa = 10.91VVV101 pKa = 3.02

Molecular weight: 11.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411BKT3|A0A411BKT3_9CAUD Uncharacterized protein OS=Vibrio phage VspSw_1 OX=2484249 GN=VspSw1_92 PE=4 SV=1
MM1 pKa = 7.56RR2 pKa = 11.84HH3 pKa = 5.68KK4 pKa = 10.73LYY6 pKa = 10.92YY7 pKa = 10.23KK8 pKa = 10.16YY9 pKa = 10.53IDD11 pKa = 3.76MLSDD15 pKa = 2.99EE16 pKa = 4.48QARR19 pKa = 11.84LRR21 pKa = 11.84GYY23 pKa = 10.14IRR25 pKa = 11.84VAKK28 pKa = 10.13NEE30 pKa = 3.94RR31 pKa = 11.84AADD34 pKa = 3.54LALRR38 pKa = 11.84RR39 pKa = 11.84FSSSRR44 pKa = 11.84ARR46 pKa = 11.84LVIATKK52 pKa = 10.77LLIARR57 pKa = 11.84YY58 pKa = 6.73TMRR61 pKa = 11.84AAAFDD66 pKa = 3.36QTINYY71 pKa = 7.91FKK73 pKa = 11.13

Molecular weight: 8.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

151

0

151

28361

40

1403

187.8

21.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.718 ± 0.331

1.185 ± 0.133

6.375 ± 0.166

7.577 ± 0.248

3.713 ± 0.123

6.403 ± 0.215

1.918 ± 0.14

5.719 ± 0.125

6.505 ± 0.273

9.062 ± 0.266

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.507 ± 0.135

4.552 ± 0.138

3.596 ± 0.132

3.512 ± 0.124

4.841 ± 0.15

6.47 ± 0.281

6.139 ± 0.28

6.985 ± 0.16

1.245 ± 0.074

3.977 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski